##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031602_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1054553 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.838194002577396 33.0 31.0 34.0 30.0 34.0 2 32.08779169942146 34.0 31.0 34.0 30.0 34.0 3 32.23920466775971 34.0 31.0 34.0 30.0 34.0 4 35.325600515099765 37.0 35.0 37.0 33.0 37.0 5 35.01250861739524 37.0 35.0 37.0 32.0 37.0 6 35.233145228357415 37.0 35.0 37.0 32.0 37.0 7 35.385608878833025 37.0 35.0 37.0 33.0 37.0 8 35.46406486919102 37.0 35.0 37.0 33.0 37.0 9 37.390812979527816 39.0 38.0 39.0 34.0 39.0 10-11 37.34406521056789 39.0 37.5 39.0 34.5 39.0 12-13 37.365446781717 39.0 38.0 39.0 34.5 39.0 14-15 38.773105761398426 41.0 39.0 41.0 35.0 41.0 16-17 38.72656234442461 41.0 38.5 41.0 34.0 41.0 18-19 38.646908690222304 41.0 38.5 41.0 34.0 41.0 20-21 38.64011529055439 40.5 39.0 41.0 34.0 41.0 22-23 38.5798727043591 40.0 38.0 41.0 34.0 41.0 24-25 38.50938549319001 40.0 38.0 41.0 34.0 41.0 26-27 38.41636740875044 40.0 38.0 41.0 34.0 41.0 28-29 38.3030378748152 40.0 38.0 41.0 34.0 41.0 30-31 38.172161569878426 40.0 38.0 41.0 33.0 41.0 32-33 38.015466742781065 40.0 38.0 41.0 33.0 41.0 34-35 37.88042990726876 40.0 38.0 41.0 33.0 41.0 36-37 37.75480559061517 40.0 38.0 41.0 32.5 41.0 38-39 37.58958724691884 40.0 37.5 41.0 31.5 41.0 40-41 37.44534366693756 40.0 37.0 41.0 31.0 41.0 42-43 37.269811948759326 40.0 37.0 41.0 31.0 41.0 44-45 37.05289871632815 40.0 37.0 41.0 30.0 41.0 46-47 36.864359591220165 40.0 36.5 41.0 30.0 41.0 48-49 37.002414767204684 40.0 37.0 41.0 30.0 41.0 50-51 36.769335443548115 39.5 36.5 40.5 30.0 41.0 52-53 36.89598673561215 39.5 36.5 40.5 30.5 41.0 54-55 37.17351712052405 40.0 37.0 41.0 31.0 41.0 56-57 37.076806950433024 40.0 36.5 41.0 30.5 41.0 58-59 36.88262846912389 40.0 36.0 41.0 30.0 41.0 60-61 36.588257299538284 39.0 35.5 41.0 29.0 41.0 62-63 36.25718811667123 39.0 35.0 41.0 28.5 41.0 64-65 35.985303251709496 39.0 35.0 41.0 28.0 41.0 66-67 35.756537604084386 38.0 35.0 40.5 28.0 41.0 68-69 35.480231434550944 37.0 35.0 40.0 28.0 41.0 70-71 35.15841830614488 37.0 35.0 39.5 28.0 41.0 72-73 34.776254488868744 36.0 34.0 39.0 28.0 41.0 74-75 34.34371245447123 36.0 34.0 39.0 26.5 40.5 76-77 33.937173380569774 35.0 34.0 37.5 26.0 39.5 78-79 33.57759828097782 35.0 34.0 37.0 26.0 39.0 80-81 33.25688040335574 35.0 34.0 37.0 26.0 39.0 82-83 32.92845072746462 35.0 33.0 36.0 26.0 38.0 84-85 32.67453034603287 35.0 33.0 36.0 25.0 37.0 86-87 32.43960284594516 35.0 33.0 35.5 25.0 37.0 88-89 32.20155316992128 35.0 33.0 35.0 24.5 36.0 90-91 32.02314155855609 35.0 33.0 35.0 24.0 36.0 92-93 31.851378261690023 35.0 32.0 35.0 24.0 36.0 94-95 31.698861982280643 35.0 32.0 35.0 23.5 36.0 96-97 31.604697440526934 35.0 32.0 35.0 23.0 35.0 98-99 31.501416239866558 35.0 32.0 35.0 23.0 35.0 100-101 31.038720197088246 34.5 31.0 35.0 20.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.32596987872069505 1101 2 0.3899267368729227 1101 3 0.42788465530506414 1101 4 0.21662476115335494 1101 5 0.1773481362920606 1101 6 0.39214801066464844 1101 7 0.3928192528323038 1101 8 0.3688781587666625 1101 9 0.18476670390272432 1101 10-11 0.44603317891542815 1101 12-13 0.28520565553761656 1101 14-15 0.4104355097950858 1101 16-17 0.37344321400235714 1101 18-19 0.34061133934200427 1101 20-21 0.14660750221197816 1101 22-23 0.3190296153850838 1101 24-25 0.4318525525077632 1101 26-27 0.3537588335295183 1101 28-29 0.2583117228606753 1101 30-31 0.2949997809818754 1101 32-33 0.4840056329999598 1101 34-35 0.37799701840802413 1101 36-37 0.40224946532236316 1101 38-39 0.37605930943824006 1101 40-41 0.3936504780803247 1101 42-43 0.443888530644692 1101 44-45 0.362494507491391 1101 46-47 0.26999005269559007 1101 48-49 0.5173469504803165 1101 50-51 0.36242917924180773 1101 52-53 0.3811906271330656 1101 54-55 0.28795171181025836 1101 56-57 0.3343953227546166 1101 58-59 0.4743391017525269 1101 60-61 0.6061337938592004 1101 62-63 0.22114466842155878 1101 64-65 0.19399490773988504 1101 66-67 0.153294759977733 1101 68-69 0.26604235911250385 1101 70-71 0.40197808241875777 1101 72-73 0.28499601133098196 1101 74-75 0.24825061936054738 1101 76-77 0.22612823223892775 1101 78-79 0.18760681382936184 1101 80-81 0.14909549182058868 1101 82-83 0.20551712879213824 1101 84-85 0.062496723210841765 1101 86-87 0.1440082186639131 1101 88-89 0.1431210517382695 1101 90-91 0.1739855347334469 1101 92-93 0.4256111764308308 1101 94-95 -0.05710929450823343 1101 96-97 -0.06423101720124791 1101 98-99 -0.126034626703607 1101 100-101 -0.034459909145684975 1102 1 0.022668329449203384 1102 2 0.05770702380995374 1102 3 0.2529011797929286 1102 4 0.13294202635991326 1102 5 0.2714986536046382 1102 6 0.20791702250647148 1102 7 0.12496505524035229 1102 8 0.17654304091265516 1102 9 0.3815625033965091 1102 10-11 0.2760882770764681 1102 12-13 0.3029752155087948 1102 14-15 0.2961058955566287 1102 16-17 0.4104853492762146 1102 18-19 0.5655124094343478 1102 20-21 0.48504885547142607 1102 22-23 0.2675451397228912 1102 24-25 0.4514060131013551 1102 26-27 0.5245873149259879 1102 28-29 0.237032646892672 1102 30-31 0.44145386770784256 1102 32-33 0.45028079641566876 1102 34-35 0.14488392890544333 1102 36-37 0.1884380937464698 1102 38-39 -0.02981621559403891 1102 40-41 0.3098238903343713 1102 42-43 0.3431536404751725 1102 44-45 -0.040017214664075595 1102 46-47 -0.503225147601043 1102 48-49 -0.006795543309820573 1102 50-51 0.06958611165200068 1102 52-53 0.15578173131608253 1102 54-55 0.11046073052230554 1102 56-57 0.06997003390160472 1102 58-59 0.2885821561971511 1102 60-61 -0.13965447691670363 1102 62-63 -0.49450888902475754 1102 64-65 -0.3288299674118704 1102 66-67 0.02915028200893488 1102 68-69 0.31413326921805407 1102 70-71 0.38296965089958235 1102 72-73 -0.025207999438144668 1102 74-75 0.15518954014097375 1102 76-77 0.18733877963661882 1102 78-79 0.20233872029022137 1102 80-81 0.23187728549329023 1102 82-83 0.19234714296661792 1102 84-85 0.18921443287342044 1102 86-87 0.11859895081343552 1102 88-89 0.0684223058631872 1102 90-91 0.10196294981990661 1102 92-93 0.12677588930157313 1102 94-95 0.10083566685608858 1102 96-97 -0.07064239448166276 1102 98-99 0.18501638032906698 1102 100-101 0.21685181896231498 1103 1 0.19171928984939868 1103 2 0.0898400955631189 1103 3 -0.05868352826832535 1103 4 0.05554794723668266 1103 5 0.13646743582482657 1103 6 0.1580294351539706 1103 7 0.23058521027910928 1103 8 0.12170667172183158 1103 9 0.27417739819163955 1103 10-11 0.2293188876847836 1103 12-13 0.13828830024549887 1103 14-15 0.25651444750987906 1103 16-17 0.39345578785260926 1103 18-19 0.25068658937095734 1103 20-21 -0.08060812604612266 1103 22-23 -0.0012469851864054249 1103 24-25 0.14041797514358478 1103 26-27 0.018794841561337705 1103 28-29 0.003729266398536879 1103 30-31 0.1351055364747893 1103 32-33 0.07712271702513362 1103 34-35 -0.1791192740196692 1103 36-37 -0.014507905222487238 1103 38-39 -0.012970428562304903 1103 40-41 0.06775184599939621 1103 42-43 -0.10257981028283325 1103 44-45 -0.29767607211242364 1103 46-47 0.10404539694395254 1103 48-49 -0.04278489394181406 1103 50-51 -0.1411058586004188 1103 52-53 -0.09607749272637989 1103 54-55 -0.12472096745760552 1103 56-57 -0.1647599848099759 1103 58-59 -0.1628751508636057 1103 60-61 -0.07012033306670418 1103 62-63 -0.12349225863563618 1103 64-65 0.1924991987774618 1103 66-67 -0.0650334971562927 1103 68-69 -0.07152087214981151 1103 70-71 -0.005431559624724969 1103 72-73 0.12939280439581324 1103 74-75 -0.038785523822063794 1103 76-77 -0.559533475680901 1103 78-79 -0.423050308617249 1103 80-81 -0.19370422918957075 1103 82-83 -0.27360328090782815 1103 84-85 -0.3197863356376729 1103 86-87 -0.42555642685487527 1103 88-89 -0.22793001112615485 1103 90-91 -0.18633491744247976 1103 92-93 -0.18615490651314204 1103 94-95 -0.2942482488272624 1103 96-97 -0.33606631665678677 1103 98-99 -0.2579562778601101 1103 100-101 -0.1772177575451117 1104 1 0.11769151715038362 1104 2 0.12590552321514537 1104 3 0.1807474422220423 1104 4 0.08425196413067937 1104 5 -0.10993756289532541 1104 6 -0.11877766434506043 1104 7 -0.15157279608356333 1104 8 0.14576781188345933 1104 9 0.288195160651874 1104 10-11 0.3380785322527444 1104 12-13 0.22606415317269324 1104 14-15 0.3529612698304305 1104 16-17 0.2962907651810127 1104 18-19 0.3352331118414327 1104 20-21 0.24211408754220543 1104 22-23 0.2702995967055699 1104 24-25 0.1548471172798358 1104 26-27 0.3021051681053848 1104 28-29 0.3573838034592782 1104 30-31 0.18708907214597303 1104 32-33 0.07757123514412001 1104 34-35 -0.005890149252785193 1104 36-37 0.03972736281422584 1104 38-39 0.040928879405662144 1104 40-41 0.17310218150237233 1104 42-43 -0.020923801397032094 1104 44-45 0.1723137805222379 1104 46-47 0.3001420894513629 1104 48-49 0.29677074744481047 1104 50-51 -0.11762007862454738 1104 52-53 -0.005576249445581993 1104 54-55 -0.08359842659388761 1104 56-57 -0.1029113359636682 1104 58-59 -0.01420025050890672 1104 60-61 0.13875941151222548 1104 62-63 0.01788293791243234 1104 64-65 0.17325862851381402 1104 66-67 0.08329230183445446 1104 68-69 0.0965094419614303 1104 70-71 7.871298110515568E-4 1104 72-73 -0.09896062559650431 1104 74-75 -0.05574807912977775 1104 76-77 -0.06404608218252861 1104 78-79 0.07945682196379522 1104 80-81 -0.0075749234156248235 1104 82-83 0.04012742995625018 1104 84-85 -0.11339886626260665 1104 86-87 -0.17913371595422234 1104 88-89 0.07285081035335139 1104 90-91 0.04229733655247969 1104 92-93 -0.016320235434047703 1104 94-95 2.836011014260009E-4 1104 96-97 0.027023099001237227 1104 98-99 0.23758710898305324 1104 100-101 0.597218195210214 1105 1 0.05870328885709242 1105 2 0.1309799669172662 1105 3 0.20991895427766138 1105 4 0.15544893777430246 1105 5 -0.23565973928237582 1105 6 -0.043499120326764285 1105 7 0.0660168649553654 1105 8 0.054973851081790315 1105 9 0.2399083848308976 1105 10-11 0.21408870729707274 1105 12-13 0.16893361158263076 1105 14-15 0.232720059375076 1105 16-17 0.21937577846110656 1105 18-19 0.18509780424142974 1105 20-21 0.2066284024650571 1105 22-23 0.16700099032480153 1105 24-25 0.24146632365690124 1105 26-27 0.25670328328087066 1105 28-29 0.26065494506742226 1105 30-31 0.11769692641701823 1105 32-33 0.33793346497849086 1105 34-35 0.17397958925424462 1105 36-37 0.18069412281025876 1105 38-39 0.27683547302184053 1105 40-41 0.0945104268194612 1105 42-43 0.28180338513127623 1105 44-45 0.05083502049132704 1105 46-47 0.20348788728666278 1105 48-49 0.2188830500281327 1105 50-51 -0.1211251067486998 1105 52-53 0.21535931841583533 1105 54-55 0.2959734499149036 1105 56-57 0.2319202079816094 1105 58-59 0.1337346056661417 1105 60-61 0.09115489551889766 1105 62-63 0.17912894489415265 1105 64-65 0.14375143590401507 1105 66-67 0.07537111265339291 1105 68-69 0.20222810896087395 1105 70-71 0.22076851479850035 1105 72-73 0.18847620270179988 1105 74-75 0.16729148649087477 1105 76-77 0.24132349883800686 1105 78-79 0.1844447298347447 1105 80-81 0.19124932274720408 1105 82-83 0.06457162251918902 1105 84-85 0.37072769402529815 1105 86-87 0.3469305712313826 1105 88-89 0.34751597444991233 1105 90-91 0.23380963194264837 1105 92-93 0.2572801237877407 1105 94-95 0.18954476602193537 1105 96-97 0.3552799905339121 1105 98-99 -0.07409991467479671 1105 100-101 0.14485758710527463 1106 1 0.11690279106987589 1106 2 0.08390638806491779 1106 3 0.013018391833441001 1106 4 -0.02686537314698967 1106 5 -0.005905015785856449 1106 6 -0.26783843537238283 1106 7 -0.012560353030963256 1106 8 0.05834503250156331 1106 9 0.19251613199024575 1106 10-11 0.24208232152120956 1106 12-13 0.2518108790607769 1106 14-15 0.2004294696847424 1106 16-17 0.24526727150509942 1106 18-19 0.30565852700632945 1106 20-21 0.14036419406252065 1106 22-23 0.31913400546233817 1106 24-25 0.42908537810711067 1106 26-27 0.3042466757140545 1106 28-29 0.2669208339372986 1106 30-31 0.3450779898044445 1106 32-33 0.2891015434768178 1106 34-35 0.3794802506820858 1106 36-37 0.29079547343464185 1106 38-39 0.2897566273389529 1106 40-41 0.4009789964446071 1106 42-43 0.2678170809400555 1106 44-45 0.3478893866835975 1106 46-47 0.664958562619347 1106 48-49 0.2618061819683888 1106 50-51 0.15175102333996904 1106 52-53 0.21520203077209032 1106 54-55 0.2550325521559671 1106 56-57 0.29447867851041565 1106 58-59 0.2678348742335146 1106 60-61 0.4152914273417281 1106 62-63 0.38556462244525846 1106 64-65 0.5048428789438546 1106 66-67 0.32066554794976554 1106 68-69 0.19294865031727682 1106 70-71 0.1997739445523976 1106 72-73 0.025019501889538276 1106 74-75 0.1975270900604258 1106 76-77 0.12712959545053337 1106 78-79 0.17647224441331133 1106 80-81 0.359236624972084 1106 82-83 0.37917867494874 1106 84-85 0.12499623475459742 1106 86-87 0.09376867972194702 1106 88-89 0.25031682165137425 1106 90-91 0.4372952695826555 1106 92-93 0.32607886630632876 1106 94-95 0.37645730691928847 1106 96-97 0.4345883142779918 1106 98-99 0.34123322260401423 1106 100-101 0.46482164149913174 1107 1 0.10854310450457305 1107 2 0.02329914728725413 1107 3 0.06965025405926184 1107 4 0.20304255930565063 1107 5 0.17640547461792977 1107 6 0.1691757174815578 1107 7 0.15148364777910928 1107 8 0.27836076654941877 1107 9 0.30640396069163955 1107 10-11 0.2953210428571964 1107 12-13 0.23823442093515013 1107 14-15 0.2909635315616086 1107 16-17 0.22811365423191887 1107 18-19 0.4817951561813061 1107 20-21 0.27563513904008374 1107 22-23 0.40318458808945223 1107 24-25 0.4157917628591079 1107 26-27 0.3088675786303057 1107 28-29 0.4581002254502593 1107 30-31 0.5530069373368534 1107 32-33 0.5587700769389272 1107 34-35 0.5241740988251564 1107 36-37 0.44166464326889354 1107 38-39 0.5380970552739015 1107 40-41 0.4537971046200795 1107 42-43 0.5490904483378571 1107 44-45 0.6837524031030924 1107 46-47 0.8447512159094757 1107 48-49 0.37282663623059875 1107 50-51 0.31274314463234276 1107 52-53 0.5702243661098265 1107 54-55 0.6135434452579105 1107 56-57 0.58384252057796 1107 58-59 0.3971666056019103 1107 60-61 0.360476380295367 1107 62-63 0.3162807747695382 1107 64-65 0.352537587786081 1107 66-67 0.3160952743954297 1107 68-69 0.2648038853932988 1107 70-71 0.3094424974873462 1107 72-73 0.4045308701285748 1107 74-75 0.4683703220831106 1107 76-77 0.5493195685001311 1107 78-79 0.587136248494815 1107 80-81 0.5058344292156036 1107 82-83 0.6056228774973462 1107 84-85 0.4938754369916367 1107 86-87 0.4863677380158151 1107 88-89 0.4255586742186708 1107 90-91 0.5541351795402818 1107 92-93 0.4989878736592708 1107 94-95 0.481883216689976 1107 96-97 0.3377786456276972 1107 98-99 0.4013244333467867 1107 100-101 0.4739305452566107 1108 1 0.04488223302569949 1108 2 0.06982937029194858 1108 3 0.0573932484841464 1108 4 0.02814892667654334 1108 5 7.360937493885444E-4 1108 6 0.11101935744634517 1108 7 0.08445844971572569 1108 8 0.07660387100951027 1108 9 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2316 100-101 -1.7285769985595287 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 2.0 7 86.0 8 387.0 9 416.0 10 355.0 11 450.0 12 638.0 13 892.0 14 1289.0 15 1561.0 16 2180.0 17 2848.0 18 3527.0 19 4435.0 20 5488.0 21 6570.0 22 7566.0 23 7850.0 24 8119.0 25 8514.0 26 9394.0 27 10645.0 28 12447.0 29 14430.0 30 17486.0 31 20891.0 32 26016.0 33 33290.0 34 46260.0 35 69345.0 36 117300.0 37 221707.0 38 298986.0 39 93034.0 40 149.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.59504781069328 18.40740274935306 13.013407574250447 23.98414186570321 2 15.234551968051289 19.32255559235698 41.27044858397006 24.172443855621673 3 19.306868604330766 23.935029026955636 29.226771309395993 27.531331059317605 4 12.13372300235227 16.13372300235227 38.57609238006985 33.1564616152256 5 13.38411637320496 37.71567581750047 35.342029762549835 13.558178046744734 6 32.33671020301125 36.9543942063026 17.58471154083694 13.12418404984921 7 28.47120947108501 31.837887190264414 21.270228235971462 18.42067510267911 8 25.565286291379905 36.69164060332674 19.533919077604782 18.20915402768858 9 25.62758355984263 16.184782185300918 19.97132729540771 38.21630695944874 10-11 24.771753223152825 26.39319970221142 28.639726650225267 20.195320424410493 12-13 25.425192567591644 23.464007733580985 28.558290441569422 22.552509257257952 14-15 22.26776696169396 25.42175600625211 26.119892250901472 26.19058478115246 16-17 22.36873888054988 28.575557212284746 26.632201215718638 22.423502691446735 18-19 22.105065441785413 27.990414962675843 28.126639364867955 21.77788023067079 20-21 22.9180367198757 27.074068928828986 28.237549327842217 21.770345023453096 22-23 22.484455499154617 27.053642633419088 27.96758436987046 22.494317497555834 24-25 22.390292379804524 27.068435631020915 28.173643240311296 22.367628748863265 26-27 22.474261606576434 27.229499133756196 27.944588844752232 22.351650414915134 28-29 22.28081471486023 27.364959371411395 27.947955199975727 22.40627071375265 30-31 22.627075168341467 27.204654483937745 27.968722292762905 22.199548054957884 32-33 22.35411591451544 27.279899635200888 27.961989582315923 22.403994867967754 34-35 22.631437206095853 27.317830398282496 27.994135904027583 22.056596491594068 36-37 22.56297217873355 27.202331224698995 27.901774495923863 22.33292210064359 38-39 22.400249205113447 27.488898139780556 27.861757540872766 22.249095114233235 40-41 22.683781659148472 27.291421104486922 27.777361593016188 22.247435643348414 42-43 22.39161995651238 27.24656797714292 27.788124447040595 22.573687619304103 44-45 22.612433771367773 27.4227726312121 27.582970071946413 22.38182352547372 46-47 22.649817120626263 27.366194921189397 27.440743566912758 22.543244391271582 48-49 22.440457999328032 27.337356898937575 27.662567172791864 22.559617928942526 50-51 22.46575121485093 27.542789037731787 27.569406158281247 22.42205358913604 52-53 22.563299803413663 27.606121270470624 27.476487220939223 22.354091705176486 54-55 22.485133531284003 27.437965622640597 27.54787142918834 22.529029416887056 56-57 22.527151455253446 27.53391825440791 27.442539170771607 22.49639111956704 58-59 22.61153872804607 27.526285864531335 27.451725797403544 22.41044961001905 60-61 22.66022576090084 27.53734114168125 27.47523647727909 22.327196620138814 62-63 22.44378348371299 27.573421774061696 27.608735328002552 22.37405941422276 64-65 22.448644347507376 27.564079434380417 27.534311481978758 22.452964736133453 66-67 22.461826472010827 27.57332227053757 27.346528628544007 22.6183226289076 68-69 22.491621808929775 27.459580568292935 27.33587764518242 22.71291997759487 70-71 22.563636743302222 27.207567120177533 27.45378042784545 22.775015708674793 72-73 22.374279289517425 27.315656837656928 27.574972633049406 22.735091239776242 74-75 22.28835055550649 27.58296984908875 27.390092905718216 22.738586689686542 76-77 22.39093782729106 27.365334281190425 27.386480491314373 22.857247400204145 78-79 22.443186674390557 27.253539852423053 27.411563043086012 22.891710430100378 80-81 22.43534958323348 27.378653676870112 27.343300297300566 22.842696442595837 82-83 22.543039870609878 27.41733250566177 27.407128985779615 22.632498637948743 84-85 22.47464643602482 27.412367821394717 27.33115857730517 22.78182716527529 86-87 22.36754820764091 27.46210341807097 27.36568339117586 22.80466498311226 88-89 22.515274499594913 27.21929354519795 27.333772199817464 22.93165975538968 90-91 22.492252595760196 27.300722711287662 27.302336753837626 22.904687939114517 92-93 22.45298172272669 27.20883433997733 27.522439920760327 22.815744016535653 94-95 22.34969522646492 27.2733142970552 27.463091492834806 22.91389898364507 96-97 22.303204714174463 27.28005288592273 27.4754802278119 22.941262172090905 98-99 22.34799183285733 27.16125361749093 27.358836594986684 23.131917954665052 100-101 22.380053395531647 27.105752474181166 27.350335702935997 23.16385842735119 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 25.0 1 19.0 2 26.5 3 50.5 4 49.0 5 40.5 6 64.0 7 142.0 8 238.0 9 295.0 10 281.0 11 271.5 12 312.0 13 346.5 14 383.5 15 422.0 16 468.5 17 551.5 18 639.5 19 733.0 20 858.0 21 1051.0 22 1281.5 23 1607.0 24 2091.5 25 2802.5 26 3757.0 27 4942.5 28 6463.5 29 8420.0 30 10657.5 31 13014.5 32 15821.0 33 19101.0 34 21998.0 35 24163.0 36 26651.5 37 29459.5 38 31733.5 39 33505.0 40 35274.0 41 37097.5 42 38709.0 43 40340.0 44 41583.0 45 42407.0 46 43121.5 47 43469.5 48 44423.5 49 44133.5 50 41757.0 51 39488.0 52 37646.5 53 36009.5 54 33944.5 55 31230.5 56 28500.0 57 25590.0 58 22571.5 59 19374.0 60 15941.5 61 12633.5 62 9467.5 63 6926.5 64 5054.5 65 3627.5 66 2573.0 67 1827.5 68 1302.5 69 942.5 70 690.0 71 545.5 72 406.5 73 299.0 74 219.5 75 170.0 76 134.5 77 107.0 78 77.0 79 50.5 80 34.0 81 20.0 82 18.0 83 17.0 84 12.0 85 12.0 86 11.0 87 6.5 88 5.0 89 4.0 90 3.5 91 4.0 92 4.0 93 3.0 94 2.0 95 1.0 96 0.5 97 1.0 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47385005779700023 2 0.3668853059068629 3 0.19752444874747882 4 0.2254983865201654 5 0.249110286538467 6 0.19904167927074315 7 0.18984346922345297 8 0.18861071942330068 9 0.18813658488478058 10-11 0.2018390730480118 12-13 0.18970122886189694 14-15 0.19989512144007937 16-17 0.08890022597252106 18-19 0.03887903215864921 20-21 1.422403615560337E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.1401067561326932 46-47 0.21051573510292987 48-49 0.08790454344162883 50-51 0.06827537354689617 52-53 0.07752099704803837 54-55 0.08757264926466474 56-57 0.11839139426847205 58-59 0.09861998401218336 60-61 0.14148174629440152 62-63 0.24171378773755325 64-65 0.1334214591395596 66-67 0.11118454928296634 68-69 0.11597330812201946 70-71 0.09435277316550235 72-73 0.07761582395574237 74-75 0.14698170694123483 76-77 0.22331736764297289 78-79 0.14631791858730664 80-81 0.08586576492599234 82-83 0.09435277316550235 84-85 0.10445183883598073 86-87 0.07870633339433865 88-89 0.10255530068190029 90-91 0.12299049929211713 92-93 0.17045136659798038 94-95 0.13996451577113714 96-97 0.09146055248052966 98-99 0.07761582395574237 100-101 0.06822796009304416 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1054553.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.95285711814145 #Duplication Level Percentage of deduplicated Percentage of total 1 78.30322975399707 54.7753464287036 2 13.607211570589376 19.037266535475194 3 4.159222930944203 8.728485822325121 4 1.7541445683883656 4.90829697428141 5 0.8251842817474262 2.886199907860694 6 0.4575613794127016 1.9204635478101855 7 0.2680587960274865 1.3126035060440253 8 0.16106419667436675 0.9013520589448167 9 0.09986478292052241 0.6287244201696166 >10 0.35151079879941066 3.7900729858428175 >50 0.010087887794409394 0.4733824724178325 >100 0.0023144721424653934 0.2682841673975582 >500 2.722902811259867E-4 0.123903877762372 >1k 2.722902811259867E-4 0.24561729496476353 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1431 0.13569730492445614 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1148 0.1088612900442178 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 9.482690770402246E-5 10-11 0.0 0.0 0.0 4.741345385201123E-5 9.482690770402246E-5 12-13 0.0 0.0 0.0 9.482690770402246E-5 9.482690770402246E-5 14-15 0.0 0.0 0.0 1.422403615560337E-4 3.318941769640786E-4 16-17 0.0 0.0 0.0 3.7930763081608985E-4 5.689614462241348E-4 18-19 0.0 0.0 0.0 5.689614462241348E-4 5.689614462241348E-4 20-21 0.0 0.0 0.0 8.060287154841909E-4 6.637883539281572E-4 22-23 0.0 0.0 0.0 0.0015646439771163706 9.008556231882134E-4 24-25 0.0 0.0 0.0 0.0028448072311206742 0.001043095984744247 26-27 0.0 0.0 0.0 0.003982730123568944 0.001043095984744247 28-29 0.0 0.0 0.0 0.0056422010083893364 0.0010905094385962582 30-31 0.0 0.0 0.0 0.010336132939738448 0.0011379228924482696 32-33 0.0 0.0 0.0 0.01958175644088064 0.0011379228924482696 34-35 0.0 0.0 0.0 0.030297197011435177 0.0011379228924482696 36-37 0.0 0.0 0.0 0.048029828752087375 0.0011379228924482696 38-39 0.0 0.0 0.0 0.07496067054002975 0.001185336346300281 40-41 0.0 0.0 0.0 0.10919318422118186 0.001232749800152292 42-43 0.0 0.0 0.0 0.14162398665595755 0.001232749800152292 44-45 0.0 0.0 0.0 0.17841682684511825 0.001232749800152292 46-47 0.0 0.0 0.0 0.22208461784282063 0.0013275767078563144 48-49 0.0 0.0 0.0 0.26911876406401575 0.001422403615560337 50-51 0.0 0.0 0.0 0.31923478478559164 0.001422403615560337 52-53 0.0 0.0 0.0 0.3694456324148715 0.0014698170694123483 54-55 0.0 0.0 0.0 0.4268159115758051 0.0015172305232643594 56-57 0.0 0.0 0.0 0.4947593909457372 0.0015172305232643594 58-59 0.0 0.0 0.0 0.5711898785551792 0.0015646439771163706 60-61 0.0 0.0 0.0 0.6497065581341099 0.001612057430968382 62-63 0.0 0.0 0.0 0.7330594100059455 0.001612057430968382 64-65 0.0 0.0 0.0 0.8131407335619927 0.001612057430968382 66-67 0.0 0.0 0.0 0.9127564001050682 0.001612057430968382 68-69 0.0 0.0 0.0 1.0266435162575993 0.001612057430968382 70-71 0.0 0.0 0.0 1.1556081107350697 0.0017068843386724042 72-73 0.0 0.0 0.0 1.2853313204741725 0.001991365061784472 74-75 0.0 0.0 0.0 1.413679540051567 0.001991365061784472 76-77 0.0 0.0 0.0 1.5539285365458162 0.0021336054233405052 78-79 0.0 0.0 0.0 1.7251385184054286 0.002275845784896539 80-81 0.0 0.0 0.0 1.9129432091132452 0.002275845784896539 82-83 0.0 0.0 0.0 2.11743743557697 0.002275845784896539 84-85 0.0 0.0 0.0 2.3201773642481696 0.0023706726926005614 86-87 0.0 0.0 0.0 2.525145725250414 0.0023706726926005614 88-89 0.0 0.0 0.0 2.765437109372407 0.0023706726926005614 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2100 0.0 28.130943 1 GTATCAA 2910 0.0 26.03071 1 GTCTACG 110 9.286678E-5 25.986061 1 GTACATG 2930 0.0 24.064453 1 TACATGG 2855 0.0 23.839554 2 TCAACGC 3110 0.0 23.688654 4 ATCAACG 3130 0.0 23.537289 3 CAACGCA 3155 0.0 23.20013 5 ACATGGG 2740 0.0 23.0712 3 TATCAAC 3310 0.0 22.431835 2 AACGCAG 3305 0.0 22.423077 6 GTGTAGC 760 0.0 21.939985 1 GTATAGG 370 0.0 21.88916 1 GTATAAG 385 0.0 21.036333 1 CATGGGG 1710 0.0 20.846552 4 GGACCGA 115 0.0037206684 20.654476 6 GTACCTG 845 0.0 20.296804 1 GTAATAC 310 2.564775E-10 19.931993 3 ACGCAGA 3685 0.0 19.85013 7 TAGCCCT 910 0.0 19.847752 4 >>END_MODULE