FastQCFastQC Report
Fri 10 Feb 2017
SRR2031602_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031602_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1054553
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16080.15248166758806814No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14650.1389214197863929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.029.7666031
GTATCAA29650.027.2437571
TAACGCG350.00838683827.1470376-77
GTACATG27200.026.9026221
TACATGG27150.025.7197482
ACATGGG27350.025.1842983
TCAACGC31800.024.7970584
CAACGCA32100.024.4126955
ATCAACG32700.024.259843
AACGCAG33200.023.8899486
TATCAAC34150.023.3688742
ACGCAGA37100.021.3786057
CGCAGAG37650.020.9401578
GGACCGT1404.8556522E-420.354486
CATGGGG16350.019.4659794
AGAGTAC31150.018.829810-11
TATACTG6600.018.7096755
TAGCCCT9800.018.419454
GTGTAGC7550.018.25131
GAGTACT21350.018.24117512-13
Produced by FastQC (version 0.11.5)