FastQCFastQC Report
Fri 10 Feb 2017
SRR2031601_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031601_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856205
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG45400.030.2265741
GGTATCA24750.028.3005181
ACATGGG43350.027.4123553
TACATGG48150.027.181392
GGACGCG1056.896988E-527.1492657
GTATCAA35750.026.7899721
TCAACGC36000.025.2195934
CAACGCA36550.024.710045
ATCAACG37650.023.9874523
AACGCAG38000.023.7556086
TATCAAC41050.022.143972
ACGCAGA42750.021.004967
CGCAGAG42950.020.68878
CGTATAC1150.00370915920.6665343
CATGGGG27200.020.6215044
TACACCG1404.8297053E-420.3718475
ACCGTAT1456.132639E-419.6624668
GTACACG2251.6195227E-619.0595341
TATTACG1507.6860795E-419.0137254
GAGTACA30850.018.5343931
Produced by FastQC (version 0.11.5)