FastQCFastQC Report
Fri 10 Feb 2017
SRR2031601_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031601_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1856205
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTCG650.005848040329.2276448
GGTATCA28250.025.9056911
GTATCAA38900.023.455491
TCAACGC36850.023.073294
GTACATG44600.023.0150451
CAACGCA37250.022.9505675
CGTACAC2103.4489858E-822.6190683
ATCAACG37700.022.5530683
GTGTACG1703.1381896E-622.3632031
AACGCAG38350.022.2922736
ACATGGG41450.022.2316253
TATACCG1553.9113103E-521.449325
TAGACGT2005.3897384E-721.3750174
TACATGG46450.021.0668162
GACGTGT2551.1945303E-820.4880056
GTGTAGC12300.020.4768751
GTATAGG6800.020.2666531
GAGTACA34550.020.0815731
TATCAAC43150.020.0358582
ACGCAGA44000.019.5376837
Produced by FastQC (version 0.11.5)