##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2031600_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	797059
Sequences flagged as poor quality	0
Sequence length	101
%GC	37
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.18398010686787	33.0	31.0	34.0	30.0	34.0
2	32.374599621859865	34.0	31.0	34.0	31.0	34.0
3	32.490306238308584	34.0	31.0	34.0	31.0	34.0
4	35.59487942548795	37.0	35.0	37.0	33.0	37.0
5	35.428131418125886	37.0	35.0	37.0	33.0	37.0
6	35.68178390809212	37.0	36.0	37.0	33.0	37.0
7	35.80128572665261	37.0	36.0	37.0	35.0	37.0
8	35.848434055697254	37.0	37.0	37.0	35.0	37.0
9	37.71617659420444	39.0	38.0	39.0	35.0	39.0
10-11	37.691394238067694	39.0	38.0	39.0	35.0	39.0
12-13	37.75845075458655	39.0	38.5	39.0	35.0	39.0
14-15	39.1035751431199	41.0	39.0	41.0	36.0	41.0
16-17	39.09160990591663	41.0	39.0	41.0	36.0	41.0
18-19	39.12669639261335	41.0	39.0	41.0	36.0	41.0
20-21	39.18821316866129	41.0	39.0	41.0	36.0	41.0
22-23	39.11358694902134	41.0	39.0	41.0	36.0	41.0
24-25	39.00131734288177	41.0	39.0	41.0	35.0	41.0
26-27	38.8845562248215	41.0	39.0	41.0	35.0	41.0
28-29	38.81415553930135	41.0	39.0	41.0	35.0	41.0
30-31	38.62929720384564	40.0	38.0	41.0	35.0	41.0
32-33	38.40571714264565	40.0	38.0	41.0	34.5	41.0
34-35	38.26759625071669	40.0	38.0	41.0	34.0	41.0
36-37	38.085637951519274	40.0	38.0	41.0	34.0	41.0
38-39	37.891980392919464	40.0	38.0	41.0	33.0	41.0
40-41	37.84719073493932	40.0	38.0	41.0	33.0	41.0
42-43	37.677803023364646	40.0	37.0	41.0	33.0	41.0
44-45	37.38558437957542	40.0	37.0	41.0	32.5	41.0
46-47	37.10143602920236	40.0	37.0	41.0	31.0	41.0
48-49	37.08316134690155	40.0	37.0	41.0	31.0	41.0
50-51	36.816462771262856	39.5	37.0	40.5	30.5	41.0
52-53	37.01478811480706	39.5	37.5	40.5	31.0	41.0
54-55	37.32075354522062	40.0	38.0	41.0	31.5	41.0
56-57	37.209776189717445	40.0	38.0	41.0	31.0	41.0
58-59	37.05122895544746	40.0	37.0	41.0	31.0	41.0
60-61	36.72976028123389	40.0	37.0	41.0	29.0	41.0
62-63	36.4838456124327	40.0	36.0	41.0	28.0	41.0
64-65	36.27505052950911	39.0	36.0	41.0	28.0	41.0
66-67	36.00190011028042	39.0	35.5	41.0	28.0	41.0
68-69	35.67028977779562	38.5	35.0	41.0	28.0	41.0
70-71	35.273185548372204	37.5	35.0	40.0	27.0	41.0
72-73	34.84658350260143	37.0	35.0	39.5	26.0	41.0
74-75	34.3838109851341	36.5	34.5	39.0	26.0	41.0
76-77	33.97405022714755	36.0	34.0	39.0	26.0	40.5
78-79	33.55994725610024	36.0	34.0	37.5	25.5	39.5
80-81	33.134303734102495	35.0	34.0	37.0	24.5	39.0
82-83	32.723521094423376	35.0	33.5	37.0	23.0	39.0
84-85	32.374469142183955	35.0	33.0	36.0	22.5	37.0
86-87	32.212147406904634	35.0	34.0	36.0	22.5	37.0
88-89	31.930503262619204	35.0	33.5	35.5	20.5	37.0
90-91	31.68861464458716	35.0	33.0	35.0	20.0	36.0
92-93	31.493751403597475	35.0	33.0	35.0	19.0	36.0
94-95	31.30286842002913	35.0	32.5	35.0	19.0	36.0
96-97	31.15591443042485	35.0	32.0	35.0	18.0	36.0
98-99	31.1128134805579	35.0	32.0	35.0	18.0	35.0
100-101	30.676287451744475	34.5	31.5	35.0	15.5	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.2146796135881317
1101	2	0.3015000656013598
1101	3	0.36610289310780075
1101	4	0.31912225501070424
1101	5	0.1828526758637068
1101	6	0.20619343000804946
1101	7	0.1266195002596575
1101	8	0.2787783876893144
1101	9	0.22424358544358114
1101	10-11	0.30294595416909686
1101	12-13	0.336626215923026
1101	14-15	0.3321831142118654
1101	16-17	0.396509624370772
1101	18-19	0.49181614550450803
1101	20-21	0.1536817583891832
1101	22-23	0.3194068641270391
1101	24-25	0.42618647299778445
1101	26-27	0.3358960298087439
1101	28-29	0.3540595455506832
1101	30-31	0.4557246458486617
1101	32-33	0.5387869982502522
1101	34-35	0.5736561774941649
1101	36-37	0.5211043510716635
1101	38-39	0.28969070885175086
1101	40-41	0.34105544431449175
1101	42-43	0.41816967882336087
1101	44-45	0.3790606728859771
1101	46-47	0.6372582264037874
1101	48-49	0.8015913688537495
1101	50-51	0.47947599874410685
1101	52-53	0.6929170624700518
1101	54-55	0.6323458427026267
1101	56-57	0.6120700593085431
1101	58-59	0.7029934285417099
1101	60-61	0.6118078839505543
1101	62-63	0.51862151146819
1101	64-65	0.712126571840578
1101	66-67	0.5900227802837463
1101	68-69	0.522899489588383
1101	70-71	0.7926139436250139
1101	72-73	0.8726356600920582
1101	74-75	0.6913340997524173
1101	76-77	0.43854361579840884
1101	78-79	0.4237500210385292
1101	80-81	0.698241244760915
1101	82-83	0.5679036723646007
1101	84-85	0.553732086389104
1101	86-87	0.7027002643358031
1101	88-89	0.65843940074312
1101	90-91	0.5938456665445813
1101	92-93	0.5810030170181584
1101	94-95	0.6072935093261229
1101	96-97	0.8499370054604363
1101	98-99	0.8790337926093628
1101	100-101	0.9133316207923698
1102	1	-0.01733291321352226
1102	2	-0.0016860302825421059
1102	3	0.07224588454182879
1102	4	0.018354909192815683
1102	5	0.06960975519659485
1102	6	0.2602060201830625
1102	7	0.19346783723695893
1102	8	0.19241369510294248
1102	9	0.18388531469144453
1102	10-11	0.17562886554144796
1102	12-13	0.21376110612867905
1102	14-15	0.20098440702849274
1102	16-17	0.13755285556735686
1102	18-19	0.18327500034781252
1102	20-21	0.058844839179656105
1102	22-23	0.036958381391002604
1102	24-25	0.17347858195772403
1102	26-27	0.161669448907368
1102	28-29	0.10779593348016903
1102	30-31	0.13674009838729972
1102	32-33	0.176935651038157
1102	34-35	-0.07301487249685579
1102	36-37	0.12762309374411984
1102	38-39	0.05171316414712379
1102	40-41	0.18717435387014092
1102	42-43	0.17760042757965522
1102	44-45	-0.5026238967560701
1102	46-47	-0.6464088733142006
1102	48-49	-0.09203775965686845
1102	50-51	-0.23116650154634755
1102	52-53	-0.026468321519722338
1102	54-55	0.07186916938175614
1102	56-57	-0.029026775191915988
1102	58-59	0.08695811901564099
1102	60-61	-0.1871757657214843
1102	62-63	-0.257818384177007
1102	64-65	0.19371792982615688
1102	66-67	0.10232117376897065
1102	68-69	0.02493139807724276
1102	70-71	4.614144996750724E-4
1102	72-73	0.030147516192272406
1102	74-75	-0.07308994198950813
1102	76-77	0.10467821026169588
1102	78-79	0.22833033093427701
1102	80-81	0.12483640514833638
1102	82-83	-0.02689627904915426
1102	84-85	-0.12922549570580344
1102	86-87	0.09672283581567598
1102	88-89	-0.032657697813018416
1102	90-91	0.03437636565841018
1102	92-93	-0.16195857872315145
1102	94-95	0.13776289471011083
1102	96-97	-0.1833802204761632
1102	98-99	-0.4151176380443289
1102	100-101	-0.17114243309611155
1103	1	0.05490875689261543
1103	2	0.16994366841963426
1103	3	0.041303367051476414
1103	4	0.24148753017309588
1103	5	0.009768021906062074
1103	6	-0.041754224605497825
1103	7	0.08967728330347313
1103	8	0.0328196038626416
1103	9	0.10876213060576134
1103	10-11	0.1595779919153273
1103	12-13	0.16352569331549915
1103	14-15	0.19204177743679907
1103	16-17	0.04940935566163063
1103	18-19	0.17750050747898882
1103	20-21	0.06014929113149492
1103	22-23	-0.06093029178773435
1103	24-25	0.10274900605747206
1103	26-27	0.07559727602671984
1103	28-29	0.03589311515057858
1103	30-31	0.20037162128399189
1103	32-33	0.24108054711511784
1103	34-35	0.2532866495336066
1103	36-37	0.22309904036350048
1103	38-39	0.219461353965265
1103	40-41	0.009993091099502749
1103	42-43	-0.07578172079156076
1103	44-45	-0.06186563310390625
1103	46-47	0.0995860515524214
1103	48-49	0.22350967704408475
1103	50-51	0.18998421870475823
1103	52-53	-0.015216149556273706
1103	54-55	0.15566746849870583
1103	56-57	0.1518818571207845
1103	58-59	0.17833090182947586
1103	60-61	0.44159810039894154
1103	62-63	0.38723437988534926
1103	64-65	0.34954643515861505
1103	66-67	0.07544742535494464
1103	68-69	0.12520732023815384
1103	70-71	0.02976827153479178
1103	72-73	0.024477481389901357
1103	74-75	-0.09644651087323552
1103	76-77	-0.5157097113285118
1103	78-79	-0.2862874289752213
1103	80-81	0.004738927938035431
1103	82-83	0.03021298408835804
1103	84-85	-0.07282833085059792
1103	86-87	0.05018931496737622
1103	88-89	0.12617843550604846
1103	90-91	0.2241193478597765
1103	92-93	-0.14537649761273386
1103	94-95	-0.01561976321265135
1103	96-97	0.03790909779482732
1103	98-99	0.3065219969129451
1103	100-101	0.08615179725175537
1104	1	-0.11505786154030773
1104	2	0.11567116212775375
1104	3	0.09697035368451878
1104	4	0.03688574504722908
1104	5	-0.01804517503563119
1104	6	-0.11028911287992571
1104	7	-0.02647051251021537
1104	8	0.1117677263285799
1104	9	-0.004248084440298783
1104	10-11	0.2047911516072034
1104	12-13	0.010157927779211207
1104	14-15	0.14137247462921465
1104	16-17	0.09042116576397063
1104	18-19	0.16357838825091875
1104	20-21	0.1682668859394454
1104	22-23	0.20316016796097358
1104	24-25	0.1990720192811537
1104	26-27	0.26706491882962524
1104	28-29	0.24825766669621174
1104	30-31	0.31984921647399744
1104	32-33	0.17096015379524943
1104	34-35	0.257897508332924
1104	36-37	0.25205719162641316
1104	38-39	0.36870059808050826
1104	40-41	0.15496173916991296
1104	42-43	0.03996731095197248
1104	44-45	0.10811411827743456
1104	46-47	0.2531448417929738
1104	48-49	0.08005012413740786
1104	50-51	-0.21531409024905201
1104	52-53	-0.0441695252015748
1104	54-55	0.10815484892918192
1104	56-57	0.009164912751955967
1104	58-59	0.048283670971351
1104	60-61	0.04044383362339232
1104	62-63	0.07666999739658564
1104	64-65	0.12863114028809974
1104	66-67	0.03963745673075891
1104	68-69	-0.15885716015078089
1104	70-71	-0.12365799531716704
1104	72-73	-0.03775000993041289
1104	74-75	-0.09985988355765585
1104	76-77	0.04225554127778253
1104	78-79	0.05094639003842616
1104	80-81	0.050251757634732996
1104	82-83	0.039595878781220506
1104	84-85	0.016821950672067487
1104	86-87	-0.16417502645106907
1104	88-89	-0.03956295524931264
1104	90-91	0.10728744482391406
1104	92-93	0.0010069271736732333
1104	94-95	-0.22810131738964756
1104	96-97	-4.7851043261104564E-4
1104	98-99	0.0014006245914686133
1104	100-101	0.12290853206648578
1105	1	0.3186788184982916
1105	2	0.3926760228604351
1105	3	0.23142728006444457
1105	4	0.2831614083369942
1105	5	0.06933420941746249
1105	6	0.18483023878894045
1105	7	0.29666206665450545
1105	8	0.3735710340779548
1105	9	0.40279943080830805
1105	10-11	0.44089888872932903
1105	12-13	0.4005145930671077
1105	14-15	0.4916034743293878
1105	16-17	0.5364672519002696
1105	18-19	0.42475468118173865
1105	20-21	0.40331209009678304
1105	22-23	0.2720759904008858
1105	24-25	0.37771871776910615
1105	26-27	0.38106343500398054
1105	28-29	0.2902632824729139
1105	30-31	0.35172301688412233
1105	32-33	0.4402394916325889
1105	34-35	0.3547652793335416
1105	36-37	0.3223452245069396
1105	38-39	0.41469919304206826
1105	40-41	0.410111905389023
1105	42-43	0.40536703563446963
1105	44-45	0.4919270687049746
1105	46-47	0.42665395812883844
1105	48-49	0.6871263091735287
1105	50-51	0.4893903811435365
1105	52-53	0.5292327907044623
1105	54-55	0.5658176703426676
1105	56-57	0.7199732037918807
1105	58-59	0.6540675270358847
1105	60-61	0.7977486109729384
1105	62-63	0.8373943581806884
1105	64-65	0.7380457732601116
1105	66-67	0.5841456604481934
1105	68-69	0.602862983329473
1105	70-71	0.5862869192972084
1105	72-73	0.5290768573809999
1105	74-75	0.6176682992811777
1105	76-77	0.6768875503295533
1105	78-79	0.6909074779145996
1105	80-81	0.7106887639836756
1105	82-83	0.5865410144374579
1105	84-85	0.6869399345075493
1105	86-87	0.580294002518535
1105	88-89	0.6640341182438263
1105	90-91	0.7530733143391757
1105	92-93	0.4884053518673319
1105	94-95	0.36467717895659746
1105	96-97	0.4944845799516351
1105	98-99	0.4394957688924208
1105	100-101	0.5313370118893026
1106	1	0.13844807841947926
1106	2	0.04468552416614102
1106	3	0.0787540541557874
1106	4	0.25812625474842577
1106	5	0.1443961812076182
1106	6	0.12466013476745275
1106	7	0.12675063920136864
1106	8	0.071440695547345
1106	9	0.0426817206478276
1106	10-11	0.06081663243843849
1106	12-13	0.0996019458517523
1106	14-15	0.07346939988868684
1106	16-17	0.05725084040344086
1106	18-19	0.16020088842534363
1106	20-21	0.11425225962095453
1106	22-23	0.11654077572065802
1106	24-25	0.03364429777742117
1106	26-27	-0.026457258500222736
1106	28-29	0.011463743870230303
1106	30-31	0.2086401455348721
1106	32-33	0.09578091902359631
1106	34-35	0.22800319923512546
1106	36-37	-0.07808469507987326
1106	38-39	0.10075227858488489
1106	40-41	0.18397440981558333
1106	42-43	0.18520720329482288
1106	44-45	0.27930843181513154
1106	46-47	0.3536960243936136
1106	48-49	0.23948185847036285
1106	50-51	-0.2710930505922349
1106	52-53	-0.19126149256614866
1106	54-55	-0.09590851064188399
1106	56-57	0.05455977895222475
1106	58-59	0.11956213129656135
1106	60-61	0.1275410443053815
1106	62-63	0.3026590921420578
1106	64-65	0.4845361093884719
1106	66-67	0.04763563002661897
1106	68-69	-0.08725839233036936
1106	70-71	-0.02314169769124419
1106	72-73	-0.10782229582652292
1106	74-75	-0.022599178627459082
1106	76-77	0.09481556986231254
1106	78-79	-0.02830698693284006
1106	80-81	0.22169872295906856
1106	82-83	0.25170075358644084
1106	84-85	0.0032072964455949204
1106	86-87	0.12416091796976048
1106	88-89	-0.013582106043315889
1106	90-91	-3.459423457385924E-5
1106	92-93	0.022722295379296042
1106	94-95	0.04851750400562693
1106	96-97	0.15442874839064658
1106	98-99	0.4905217980797332
1106	100-101	0.4988787043559775
1107	1	-0.003049338883421626
1107	2	-0.13801001452743122
1107	3	-0.045307227268331474
1107	4	-0.0355940012550775
1107	5	0.05263424258281191
1107	6	0.012457379369578803
1107	7	-0.0933139086898862
1107	8	0.06792807119887811
1107	9	0.061827281612764295
1107	10-11	0.05289564499636157
1107	12-13	0.023698587954790185
1107	14-15	0.0911976032536117
1107	16-17	0.14687550926337423
1107	18-19	0.2946544975589447
1107	20-21	0.07858388418041073
1107	22-23	0.243444586859475
1107	24-25	0.10627044358064808
1107	26-27	0.25693806998290114
1107	28-29	0.2819436332167058
1107	30-31	0.318600135219711
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2313	1	-0.09994561678228564
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2313	4	-0.6830531842963978
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2314	4	-0.8868546150199634
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2314	10-11	-0.26914961323796405
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2314	98-99	-0.272640232409028
2314	100-101	-0.15571804482589258
2315	1	-0.44357042917719625
2315	2	-0.5267482665508361
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2315	4	-1.4855440532417816
2315	5	-0.4242622529014781
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2315	7	-0.2653797177457662
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2315	9	-0.27239628161733975
2315	10-11	-0.19488217808573438
2315	12-13	-0.6022468565825321
2315	14-15	-0.7936416456913591
2315	16-17	-0.5679187864696331
2315	18-19	-1.0612098069404468
2315	20-21	-0.6470409756593511
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2315	24-25	-0.4192718491351073
2315	26-27	-1.0922979292831627
2315	28-29	-0.6144346261460001
2315	30-31	-0.456279581809504
2315	32-33	-0.5063670791183412
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2316	1	-0.143505538764515
2316	2	-0.2695239620243228
2316	3	-0.4938711498719286
2316	4	-0.19984867660297567
2316	5	-0.018021635377195366
2316	6	-0.07488546658683504
2316	7	-0.1311082175504552
2316	8	-0.20002289625615788
2316	9	-0.2859393304336564
2316	10-11	-0.3613886364574057
2316	12-13	-0.4727287405352243
2316	14-15	-0.6878061089895411
2316	16-17	-1.1819915315277925
2316	18-19	-0.8816824900137377
2316	20-21	-0.5976370957937789
2316	22-23	-0.4865694790618491
2316	24-25	-1.1241726087726178
2316	26-27	-1.1789351047100425
2316	28-29	-1.0197099584268798
2316	30-31	-0.901317644394851
2316	32-33	-1.0372618565700478
2316	34-35	-1.1645393171087406
2316	36-37	-0.9285959554297563
2316	38-39	-0.9455303024291268
2316	40-41	-0.8216771114670323
2316	42-43	-0.8525937646534842
2316	44-45	-1.129895368204913
2316	46-47	-0.8772020935396654
2316	48-49	-0.956754131832831
2316	50-51	-1.606862960541612
2316	52-53	-1.3332326075629126
2316	54-55	-1.388928195062519
2316	56-57	-1.3303240412633244
2316	58-59	-1.4492368183396778
2316	60-61	-1.2020656508973246
2316	62-63	-0.8290026679597062
2316	64-65	-1.0478397941186373
2316	66-67	-1.454852301556187
2316	68-69	-1.616999724168842
2316	70-71	-1.302324002873398
2316	72-73	-1.2458700151091335
2316	74-75	-1.2353423877065168
2316	76-77	-1.0119229442590694
2316	78-79	-1.2438741004495952
2316	80-81	-1.2965050659237534
2316	82-83	-1.4032792551005926
2316	84-85	-2.0860816661964563
2316	86-87	-1.4163377325702093
2316	88-89	-1.0808750558107363
2316	90-91	-1.2071101949330014
2316	92-93	-1.2378794115855491
2316	94-95	-1.3928122307676247
2316	96-97	-1.0862589874245536
2316	98-99	-0.8736901011754341
2316	100-101	-1.4398678137154022
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	40.0
8	220.0
9	266.0
10	296.0
11	367.0
12	508.0
13	589.0
14	893.0
15	1228.0
16	2017.0
17	3021.0
18	3702.0
19	4730.0
20	6182.0
21	7597.0
22	7418.0
23	6456.0
24	5621.0
25	5368.0
26	5794.0
27	6786.0
28	7799.0
29	8894.0
30	10278.0
31	12474.0
32	15458.0
33	20511.0
34	28956.0
35	44764.0
36	70733.0
37	143186.0
38	269720.0
39	95158.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.57209411960455	25.354360285933613	14.731429882356798	19.342115712105034
2	12.989057207258256	24.909271781698212	41.12714542958961	20.974525581453918
3	17.361877584884787	32.060993852845414	33.43285270713648	17.144275855133316
4	9.236305930314002	20.583260924510817	46.715573534460106	23.46485961071508
5	8.502807981837734	43.64955883555226	37.88693863946519	9.960694543144815
6	21.982810097716186	45.29842218470408	20.167775658826827	12.550992058752907
7	21.817130502551727	36.547376624682606	27.533499258365385	14.101993614400282
8	22.790261513166165	35.370020263373576	23.33593958196107	18.50377864149919
9	24.321226016652588	18.947221551828164	26.330522706246732	30.401029725272515
10-11	21.436069637981063	28.466498539151246	32.26772253428343	17.829709288584258
12-13	23.663658190137394	28.995449574497506	30.051852224303293	17.289040011061807
14-15	21.681525235261542	30.005047492314045	30.086071372672762	18.22735589975165
16-17	17.48873151978663	32.8298749872074	31.03526866354789	18.64612482945809
18-19	16.88553498588014	33.32751804204581	32.87819265767179	16.90875431440226
20-21	18.478175637486174	31.386984486043335	31.835635240412692	18.299204636057794
22-23	18.537899954708497	30.820491331256534	31.725192237964816	18.916416476070154
24-25	19.298759564850283	31.874428367285233	31.253207102610975	17.573604965253516
26-27	18.630302148272587	29.82928490864541	34.18360497779964	17.35680796528237
28-29	17.111844919886735	31.418627730193123	33.779557096777026	17.689970253143116
30-31	18.439977467163658	31.12335473283659	31.8736128693108	18.563054930688946
32-33	18.444556801943143	31.162247713155487	32.64074554079435	17.752449944107024
34-35	19.370523386599988	30.688506120625952	32.47501126014511	17.465959232628954
36-37	19.316700520287707	29.101296139934433	33.38592249758173	18.196080842196125
38-39	17.983298601483703	32.293029750620725	32.76708499621734	16.956586651678233
40-41	17.438169570884966	31.740561238252123	33.39288559567108	17.428383595191825
42-43	17.60885956999419	31.60211477443953	30.89125146319156	19.89777419237472
44-45	18.781750441451667	30.38173712409079	31.64990165882806	19.186610775629482
46-47	19.638613211039853	31.501571096599655	31.785053619682106	17.074762072678382
48-49	17.901285726663478	31.383835682148376	31.956378969786243	18.758499621401906
50-51	16.11583632015366	31.103941347427362	32.72649095166059	20.053731380758393
52-53	18.009174018206163	32.335118460592966	30.800821355236142	18.854886165964732
54-55	17.730051643669977	30.74411835980809	31.27662700161598	20.24920299490595
56-57	18.968121242645427	30.42457910406895	30.81713689660175	19.790162756683877
58-59	19.934912405509653	29.961202368671053	30.642845168002726	19.461040057816568
60-61	19.47626029068314	31.70353284664197	31.490275594953182	17.32993126772171
62-63	17.48085670414632	31.46046150150845	32.5954146634691	18.463267130876133
64-65	17.373318375791897	32.41469239263295	30.900241322260065	19.311747909315088
66-67	18.333577188241964	30.708198611345722	29.74793356513155	21.21029063528076
68-69	17.814763087759136	30.97273053823025	30.5406793753344	20.671826998676217
70-71	19.38483233544965	30.473710118138715	31.19719671702966	18.94426082938198
72-73	19.08066960378972	29.408917587708782	32.25905274642768	19.25136006207382
74-75	17.508874353229096	32.32777221854748	30.335055124322608	19.82829830390082
76-77	17.668379614351043	31.074882529505132	31.585372369682396	19.67136548646143
78-79	18.86285545581889	30.92778130241148	30.596242391502354	19.61312085026728
80-81	19.511394250419237	30.30618849970837	30.31974972014287	19.86266752972952
82-83	19.1076392006374	30.48196197822717	31.814337620132616	18.596061201002815
84-85	18.15123306594666	31.132222972230505	31.087389918842128	19.629154042980705
86-87	17.47894316899892	31.66726409035808	31.599339841589604	19.254452899053394
88-89	18.762746260259796	30.598810516480977	29.769864073378272	20.86857914988095
90-91	18.644567020221654	29.854116961407474	31.355275974484755	20.14604004388612
92-93	20.391888784310375	31.043796895400405	30.644820787472028	17.9194935328172
94-95	19.423215953579902	29.400421715390472	31.0527370358948	20.123625295134822
96-97	18.97533552327567	29.371785405742067	32.1261025053744	19.526776565607857
98-99	18.968315201935578	30.86497722092097	29.915331007609346	20.251376569534106
100-101	18.27034193061206	29.513885183676365	30.98861905177387	21.22715383393771
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	799.0
1	509.0
2	448.0
3	628.0
4	449.0
5	434.0
6	804.5
7	1397.5
8	2085.5
9	2432.0
10	1938.5
11	1313.0
12	1225.0
13	1268.5
14	1229.0
15	1182.0
16	1160.0
17	1165.0
18	1214.0
19	1254.5
20	1320.0
21	1437.0
22	1560.5
23	1849.5
24	2120.0
25	2613.5
26	3766.5
27	5253.5
28	6175.0
29	7964.0
30	12295.0
31	16665.0
32	24920.0
33	35739.5
34	47049.0
35	55618.0
36	63159.0
37	67738.5
38	60269.0
39	52643.0
40	43705.5
41	33856.0
42	27591.5
43	21739.5
44	19164.0
45	18156.5
46	16405.5
47	21428.0
48	36117.0
49	32736.5
50	15824.5
51	7244.0
52	2818.5
53	1643.5
54	1187.0
55	874.5
56	655.5
57	513.5
58	453.5
59	393.0
60	325.0
61	269.0
62	233.5
63	218.0
64	185.5
65	129.0
66	94.5
67	67.0
68	45.0
69	28.5
70	21.0
71	26.0
72	21.5
73	17.0
74	13.5
75	23.0
76	24.5
77	10.0
78	15.5
79	26.5
80	25.5
81	13.5
82	5.0
83	4.5
84	4.0
85	3.5
86	2.5
87	2.0
88	1.5
89	0.5
90	1.0
91	1.0
92	0.5
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.46771945364144935
2	0.3674759334001623
3	0.19684866490435465
4	0.2265829756642858
5	0.2514242985776461
6	0.19973427312156314
7	0.190575603562597
8	0.19258298319195943
9	0.18907006884057517
10-11	0.2046272609681341
12-13	0.19183021583094853
14-15	0.20255715072535407
16-17	0.08788558939802449
18-19	0.0387675190920622
20-21	3.1365306708788185E-4
22-23	0.0
24-25	0.0
26-27	0.0
28-29	0.0
30-31	0.0
32-33	0.0
34-35	0.0
36-37	0.0
38-39	0.0
40-41	0.0
42-43	0.0
44-45	0.13869738626626132
46-47	0.20017338741548618
48-49	0.08782285878460691
50-51	0.06323245832491697
52-53	0.07157562990945464
54-55	0.08029518517449775
56-57	0.10921399796000045
58-59	0.09378226705927667
60-61	0.1344944351672837
62-63	0.2348634166354059
64-65	0.128974141186537
66-67	0.10294093661824281
68-69	0.1075202713977259
70-71	0.08870108737245298
72-73	0.0737712013790698
74-75	0.13618816172955828
76-77	0.20657190998407898
78-79	0.13474535762095402
80-81	0.08412175259296992
82-83	0.08776012817118933
84-85	0.09679333650332034
86-87	0.07320662585831161
88-89	0.09371953644585908
90-91	0.1138560633529011
92-93	0.15958668053431427
94-95	0.12853502689261398
96-97	0.08669370774309054
98-99	0.07584131162184983
100-101	0.06379703384567516
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	797059.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.639075008010591
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.69992044427326	10.9353773847614
2	8.760666398515454	2.5649610505605147
3	3.4841963481120466	1.5301623504794648
4	1.9402697771443336	1.1361501921356756
5	1.2446836641195884	0.9110508760146057
6	0.9669762488158176	0.8493382702427678
7	0.7316749561373509	0.7497731195064277
8	0.619636709108666	0.725672660988688
9	0.47743868564353836	0.6290334657775573
>10	4.935542955025697	15.743621965098065
>50	1.0324393989117318	10.680824553139946
>100	0.9600479886374533	28.63249732029297
>500	0.10169269538529578	10.424719925945912
>1k	0.04136652015673049	10.743021391172784
>5k	0.0025854075097956554	2.2170081840791895
>10k+	8.618025032652184E-4	1.5267872898040376
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT	12102	1.5183317671590182	No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT	7083	0.8886418696733869	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5414	0.6792470820855169	No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT	5076	0.6368411874152353	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4779	0.5995792030451949	No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC	3921	0.49193347042063384	No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT	3582	0.4494021145235171	No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC	3209	0.4026050769140051	No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC	3158	0.39620655434541235	No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT	2951	0.37023608039053574	No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC	2657	0.3333504797010008	No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA	2555	0.3205534345638152	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2526	0.3169150589855958	No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC	2433	0.3052471648899266	No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC	2375	0.29797041373348776	No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC	2367	0.2969667239188065	No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT	2076	0.26045750690977704	No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG	1959	0.2457785433700642	No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC	1940	0.2433947800601963	No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA	1915	0.2402582493893175	No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA	1825	0.22896673897415373	No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC	1756	0.2203099143225282	No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC	1631	0.2046272609681341	No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC	1537	0.19283390564562974	No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT	1494	0.18743907289171816	No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA	1471	0.18455346467450967	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1399	0.17552025634237867	No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT	1390	0.1743911053008623	No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT	1349	0.16924719500062102	No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT	1327	0.1664870480102477	No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC	1280	0.1605903703489955	No Hit
ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT	1267	0.15895937440013852	No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC	1248	0.1565756110902706	No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC	1244	0.15607376618292998	No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT	1238	0.15532099882191908	No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT	1230	0.15431730900723784	No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA	1227	0.1539409253267324	No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA	1226	0.15381546409989727	No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC	1179	0.14791878643864506	No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG	1166	0.14628779048978807	No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT	1157	0.1451586394482717	No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG	1151	0.1444058720872608	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	1144	0.14352764349941471	No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC	1134	0.1422730312310632	No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG	1132	0.14202210877739288	No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA	1127	0.14139480264321713	No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA	1116	0.14001472914803045	No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG	1100	0.138007349518668	No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG	1095	0.13738004338449222	No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG	1063	0.13336528412576737	No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA	1042	0.13073059836222914	No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT	1006	0.12621399419616366	No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC	966	0.12119554512275753	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	962	0.12069370021541694	No Hit
GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC	957	0.12006639408124117	No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT	934	0.11718078586403266	No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC	929	0.1165534797298569	No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA	920	0.11542432868834052	No Hit
CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG	920	0.11542432868834052	No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA	918	0.11517340623467019	No Hit
GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC	911	0.11429517764682413	No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT	903	0.1132914878321429	No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC	902	0.11316602660530777	No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC	900	0.11291510415163747	No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC	893	0.11203687556379138	No Hit
CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC	888	0.11140956942961562	No Hit
ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT	888	0.11140956942961562	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	877	0.11002949593442894	No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT	871	0.10927672857341804	No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC	868	0.10890034489291257	No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG	868	0.10890034489291257	No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA	865	0.10852396121240711	No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT	860	0.10789665507823135	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	857	0.1075202713977259	No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT	850	0.10664204280987982	No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT	847	0.10626565912937437	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	845	0.10601473667570405	No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA	843	0.10576381422203376	No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC	840	0.10538743054152828	No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA	836	0.10488558563418768	No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT	828	0.10388189581950646	No Hit
ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA	825	0.103505512139001	No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC	823	0.1032545896853307	No Hit
CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG	822	0.10312912845849553	No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT	808	0.10137267128280339	No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA	807	0.10124721005596825	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.2546122683515274E-4	0.0
2	0.0	0.0	0.0	1.2546122683515274E-4	0.0
3	0.0	0.0	0.0	1.2546122683515274E-4	0.0
4	0.0	0.0	0.0	1.2546122683515274E-4	0.0
5	0.0	0.0	0.0	1.2546122683515274E-4	0.0
6	0.0	0.0	0.0	1.2546122683515274E-4	0.0
7	0.0	0.0	0.0	2.5092245367030547E-4	0.0
8	0.0	0.0	0.0	2.5092245367030547E-4	0.0
9	0.0	0.0	0.0	3.763836805054582E-4	0.0
10-11	0.0	0.0	0.0	0.0012546122683515272	0.0
12-13	0.0	0.0	0.0	0.0040147592587248876	0.0
14-15	0.0	0.0	0.0	0.006586714408845519	0.0
16-17	0.0	0.0	0.0	0.013361620657943765	0.0
18-19	0.0	0.0	0.0	0.023335788191338407	0.0
20-21	0.0	0.0	0.0	0.03707379252978764	0.0
22-23	0.0	0.0	0.0	0.059970466427203	0.0
24-25	0.0	0.0	0.0	0.09045754454814511	0.0
26-27	0.0	0.0	0.0	0.10494831624760526	0.0
28-29	0.0	0.0	0.0	0.12025458592149389	0.0
30-31	0.0	0.0	0.0	0.1440922190201729	0.0
32-33	0.0	0.0	0.0	0.17483021959478534	0.0
34-35	0.0	0.0	0.0	0.22595566953011006	0.0
36-37	0.0	0.0	0.0	0.27726931130568755	0.0
38-39	0.0	0.0	0.0	0.34520656563692276	0.0
40-41	0.0	0.0	0.0	0.4334058081020351	0.0
42-43	0.0	0.0	0.0	0.5088080054299619	0.0
44-45	0.0	0.0	0.0	0.5889149987642068	0.0
46-47	0.0	0.0	0.0	0.6871511393761315	0.0
48-49	0.0	0.0	0.0	0.7830035166781881	0.0
50-51	0.0	0.0	0.0	0.8871990655647826	0.0
52-53	0.0	0.0	0.0	0.9817340999850701	0.0
54-55	0.0	0.0	0.0	1.0809111997982583	0.0
56-57	0.0	0.0	0.0	1.2044905082308839	0.0
58-59	0.0	0.0	0.0	1.3332137269637505	0.0
60-61	0.0	0.0	0.0	1.4702801172811548	0.0
62-63	0.0	0.0	0.0	1.6080992749595702	0.0
64-65	0.0	0.0	0.0	1.7515641878455672	0.0
66-67	0.0	0.0	0.0	1.9158556643861997	0.0
68-69	0.0	0.0	0.0	2.103859312798676	0.0
70-71	0.0	0.0	0.0	2.3279330639262588	0.0
72-73	0.0	0.0	0.0	2.550877664012325	0.0
74-75	0.0	0.0	0.0	2.775390529433831	0.0
76-77	0.0	0.0	0.0	3.0080583745996217	0.0
78-79	0.0	0.0	0.0	3.314873804824988	0.0
80-81	0.0	0.0	0.0	3.6624641337717785	0.0
82-83	0.0	0.0	0.0	3.999452989050998	0.0
84-85	0.0	0.0	0.0	4.309279488720408	0.0
86-87	0.0	0.0	0.0	4.639367976523696	0.0
88-89	0.0	0.0	0.0	5.008537636486132	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCCAGC	15	6.6099345E-4	95.02615	9
TGTAGTC	20	0.0020695121	71.287544	5
GACATAT	340	0.0	65.82469	1
GATATAC	40	7.5310454E-6	59.391346	8
TACCTGG	65	6.6211214E-10	58.547577	2
TATAACG	25	0.004991568	57.08388	2
ACATATA	430	0.0	51.99501	2
CTTTGCG	60	1.476661E-6	47.569904	2
CATTCCG	170	0.0	47.513077	9
GTACTAA	50	2.821609E-5	47.513077	9
TGGGAGT	50	2.8237071E-5	47.507103	6
TGGACTG	55	4.9465234E-5	43.204575	5
ATGTATT	45	9.506612E-4	42.326992	1
TAGGCAT	195	0.0	41.43208	5
TTAGGCA	200	0.0	40.396275	4
GGCATTC	215	0.0	39.773388	7
ACCTTAA	110	1.5788828E-9	38.96007	1
ATACTCA	185	0.0	38.51927	6
CATATAG	580	0.0	38.50924	3
GGGAGCC	150	0.0	38.01046	8
>>END_MODULE