##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031600_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 797059 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45766624553515 34.0 31.0 34.0 31.0 34.0 2 32.651935427615776 34.0 31.0 34.0 31.0 34.0 3 32.735251719132464 34.0 31.0 34.0 31.0 34.0 4 36.17985117789273 37.0 35.0 37.0 35.0 37.0 5 35.94359263241491 37.0 35.0 37.0 35.0 37.0 6 36.05383290321043 37.0 37.0 37.0 35.0 37.0 7 36.00833815313546 37.0 37.0 37.0 35.0 37.0 8 36.11821709559769 37.0 37.0 37.0 35.0 37.0 9 37.94494384982793 39.0 39.0 39.0 35.0 39.0 10-11 37.92009688115936 39.0 38.0 39.0 35.0 39.0 12-13 37.966646760152 39.0 38.5 39.0 35.0 39.0 14-15 39.282225657071805 41.0 39.0 41.0 36.0 41.0 16-17 39.27568661792917 41.0 39.0 41.0 36.0 41.0 18-19 39.40147404395409 41.0 39.0 41.0 36.0 41.0 20-21 39.450853700918 41.0 39.0 41.0 36.0 41.0 22-23 39.37332242657068 41.0 39.0 41.0 36.0 41.0 24-25 39.2721668032103 41.0 39.0 41.0 36.0 41.0 26-27 39.117563442605885 41.0 39.0 41.0 35.5 41.0 28-29 39.06379766115181 41.0 39.0 41.0 35.0 41.0 30-31 38.91679787819973 40.0 38.0 41.0 35.0 41.0 32-33 38.68019619626652 40.0 38.0 41.0 35.0 41.0 34-35 38.51676789296651 40.0 38.0 41.0 35.0 41.0 36-37 38.32790169861955 40.0 38.0 41.0 34.5 41.0 38-39 38.1898542015083 40.0 38.0 41.0 34.0 41.0 40-41 38.144749008542654 40.0 38.0 41.0 34.0 41.0 42-43 37.978381776004035 40.0 38.0 41.0 33.0 41.0 44-45 37.841022433721974 40.0 38.0 41.0 33.0 41.0 46-47 37.742655186128005 40.0 38.0 41.0 33.0 41.0 48-49 37.73238869393608 40.0 38.0 41.0 33.0 41.0 50-51 37.630480303214696 40.0 38.0 41.0 33.0 41.0 52-53 37.65044996669005 40.0 38.0 41.0 33.0 41.0 54-55 37.571230611535654 40.0 38.0 41.0 33.0 41.0 56-57 37.443078868691025 40.0 38.0 41.0 32.0 41.0 58-59 37.28024587891235 40.0 38.0 41.0 31.5 41.0 60-61 36.95733000944723 40.0 37.0 41.0 31.0 41.0 62-63 36.814287273589535 40.0 36.5 41.0 31.0 41.0 64-65 36.56382212609104 39.0 36.0 41.0 30.0 41.0 66-67 36.249577509318634 39.0 36.0 41.0 29.5 41.0 68-69 35.88621482224026 38.5 35.0 40.5 29.0 41.0 70-71 35.45481890299212 37.5 35.0 40.0 28.5 41.0 72-73 35.03720239028729 37.0 35.0 39.0 28.5 41.0 74-75 34.6469452073184 36.5 35.0 39.0 28.0 41.0 76-77 33.99163989114984 36.0 34.0 38.5 26.5 40.0 78-79 33.8854720917774 36.0 34.0 37.0 26.5 39.5 80-81 33.525089108836355 35.0 34.0 37.0 26.0 39.0 82-83 33.16877608809386 35.0 34.0 37.0 26.0 39.0 84-85 32.82285690268851 35.0 34.0 36.0 26.0 37.0 86-87 32.644895798178055 35.0 34.0 36.0 26.0 37.0 88-89 32.3821203951025 35.0 34.0 35.5 25.0 37.0 90-91 32.18792021669663 35.0 34.0 35.0 25.0 36.0 92-93 32.03767600641859 35.0 33.0 35.0 24.5 36.0 94-95 31.861243646957128 35.0 33.0 35.0 24.0 36.0 96-97 31.707493422695183 35.0 33.0 35.0 23.0 36.0 98-99 31.685763538207333 35.0 33.0 35.0 23.0 35.5 100-101 31.263654258969538 34.5 32.0 35.0 20.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1867163783989625 1101 2 0.276215451546868 1101 3 0.059443824662018585 1101 4 0.03043834457042749 1101 5 0.17415580185360824 1101 6 0.08913606202040114 1101 7 0.3232126669775397 1101 8 0.25638062878140744 1101 9 0.1312562576699321 1101 10-11 0.16673352917997875 1101 12-13 0.20136713016626118 1101 14-15 0.20557238406919254 1101 16-17 0.19197171324361761 1101 18-19 0.3246268688871652 1101 20-21 0.14193783966775442 1101 22-23 0.11200985658140894 1101 24-25 0.13982998895333054 1101 26-27 0.23115144499544726 1101 28-29 -0.10997363048859654 1101 30-31 -0.14366073924441025 1101 32-33 -0.3818653269716705 1101 34-35 -0.15692563719522923 1101 36-37 -0.11138316771679513 1101 38-39 -0.06827998950521419 1101 40-41 -0.11467016111648576 1101 42-43 -0.1352635193181797 1101 44-45 -0.08876396423483612 1101 46-47 0.030431756522958153 1101 48-49 -0.17787354593759375 1101 50-51 -0.03178350041837774 1101 52-53 -0.15424859176342665 1101 54-55 -0.012900502707687167 1101 56-57 0.18077593523053537 1101 58-59 0.10221785260326754 1101 60-61 -0.021166747004293995 1101 62-63 0.07104407779403488 1101 64-65 0.16026981346978886 1101 66-67 0.19895475483472325 1101 68-69 0.16713991025695663 1101 70-71 0.24409088827588477 1101 72-73 0.18658057484113755 1101 74-75 -0.21177435329671823 1101 76-77 -0.12461154846889144 1101 78-79 -0.020838422504468213 1101 80-81 -0.008837446895356038 1101 82-83 -0.11539058665983504 1101 84-85 0.09504112980212653 1101 86-87 -0.22347331687731753 1101 88-89 -0.08534841549963801 1101 90-91 -0.05654889508425498 1101 92-93 -0.31683854425267555 1101 94-95 0.04012484693203078 1101 96-97 0.263934293842091 1101 98-99 0.23734641509187426 1101 100-101 0.17623217058469365 1102 1 0.09256732424642422 1102 2 0.1429122205615201 1102 3 0.21973618711541576 1102 4 0.17449920412448705 1102 5 0.03312366099574149 1102 6 0.05588881084006658 1102 7 0.07429265472536173 1102 8 -0.01717577879382759 1102 9 0.1200180477562256 1102 10-11 0.11771159143374632 1102 12-13 0.13038236089812472 1102 14-15 0.14915952772604868 1102 16-17 0.28572204331322837 1102 18-19 0.30038127597653386 1102 20-21 0.16886359777162596 1102 22-23 0.12097982816901975 1102 24-25 0.17819067845552894 1102 26-27 0.22580299711475504 1102 28-29 -0.1839158234182463 1102 30-31 -0.17699644907890644 1102 32-33 0.23819023034425157 1102 34-35 0.014099268536895693 1102 36-37 0.09547145378579103 1102 38-39 0.09036202492278278 1102 40-41 0.11760906192606768 1102 42-43 0.19450638255873542 1102 44-45 0.12930114133462922 1102 46-47 -0.06296341865158439 1102 48-49 -0.11796459233745793 1102 50-51 0.035569182915480724 1102 52-53 -0.26378681118313807 1102 54-55 -0.25362158596973217 1102 56-57 -0.4243248284184986 1102 58-59 -0.3730546528626846 1102 60-61 0.026763320488335296 1102 62-63 0.03836322605601339 1102 64-65 -0.0247790896428981 1102 66-67 -0.23024663515439414 1102 68-69 -0.2994715687717502 1102 70-71 -0.29065091807623844 1102 72-73 -0.032887986689111415 1102 74-75 -0.06756562255957732 1102 76-77 0.04555517629002992 1102 78-79 0.042257683202301166 1102 80-81 -0.09196085595986148 1102 82-83 -0.4005944904590706 1102 84-85 -0.18168130542501615 1102 86-87 -0.025007744457397507 1102 88-89 -0.13944814405039807 1102 90-91 -0.03187817252228342 1102 92-93 -0.023305003899686483 1102 94-95 -0.16036207174938255 1102 96-97 -0.35156630292375723 1102 98-99 -0.015482941562858343 1102 100-101 -0.2732856637362069 1103 1 0.15742814024225993 1103 2 0.20497050306708076 1103 3 0.1654358268624776 1103 4 0.1719285165865898 1103 5 0.14016515377794292 1103 6 0.19348301418170877 1103 7 0.22461545562828178 1103 8 0.11723372739977123 1103 9 0.21286343453132162 1103 10-11 0.11146831114472633 1103 12-13 0.15298097186061455 1103 14-15 0.3145000865002672 1103 16-17 0.44170723574536197 1103 18-19 0.2784438081419722 1103 20-21 0.18752847081685076 1103 22-23 0.1362307058715473 1103 24-25 0.2756576977318659 1103 26-27 0.1816809289469532 1103 28-29 0.22174198214707985 1103 30-31 0.22765244442992127 1103 32-33 0.23735583969146745 1103 34-35 0.2714406324025589 1103 36-37 0.3055047784567506 1103 38-39 0.4471602068428808 1103 40-41 0.3809236437625927 1103 42-43 0.28992373075058 1103 44-45 0.2986849916080061 1103 46-47 0.44539959605916835 1103 48-49 0.3048243361266074 1103 50-51 0.4281111317463129 1103 52-53 0.3351617292064688 1103 54-55 0.4698396957562281 1103 56-57 0.4472185012970158 1103 58-59 0.593334053436898 1103 60-61 0.6090699074611408 1103 62-63 0.5414358218644466 1103 64-65 0.7349979306015868 1103 66-67 0.7411547844562065 1103 68-69 0.546641317277242 1103 70-71 0.5843306106803041 1103 72-73 0.6844726891800548 1103 74-75 0.6538816566899541 1103 76-77 0.3044952581240068 1103 78-79 0.4703650559035921 1103 80-81 0.5509900079847796 1103 82-83 0.42578855981837904 1103 84-85 0.4320595162778105 1103 86-87 0.2835855875511015 1103 88-89 0.5143701804422136 1103 90-91 0.5731085536600062 1103 92-93 0.3530115499013178 1103 94-95 0.5852341290343048 1103 96-97 0.7217607119704397 1103 98-99 0.3275305972216529 1103 100-101 0.5933978181314039 1104 1 0.017349724601160688 1104 2 0.09263365010357205 1104 3 0.018936593349103248 1104 4 0.040849752325406996 1104 5 -0.05108400451945272 1104 6 0.06242574019974967 1104 7 -0.03525455206045791 1104 8 0.1040577984227582 1104 9 0.11390308648431358 1104 10-11 0.017783826648766876 1104 12-13 0.12266535129298006 1104 14-15 0.17661248543626584 1104 16-17 0.21896168542706818 1104 18-19 0.059394585851300974 1104 20-21 0.05939148700142738 1104 22-23 0.12624727726464613 1104 24-25 0.13842555018437963 1104 26-27 0.14965563727611197 1104 28-29 0.06861777310874118 1104 30-31 0.06660787359079734 1104 32-33 0.25902401067767755 1104 34-35 0.33170892659970264 1104 36-37 0.20504369257682242 1104 38-39 0.12408728793764112 1104 40-41 0.19747903853902216 1104 42-43 0.2547262350844548 1104 44-45 0.4089122154937286 1104 46-47 0.32436037391175887 1104 48-49 0.5943425718224375 1104 50-51 0.8050565989688678 1104 52-53 0.6464448421450442 1104 54-55 0.6981810760052198 1104 56-57 0.5306568982176927 1104 58-59 0.4680636340899227 1104 60-61 0.6665170055571039 1104 62-63 0.5020632425056206 1104 64-65 0.5281993613766147 1104 66-67 0.5700730593121008 1104 68-69 0.5383460694943167 1104 70-71 0.5090286722571165 1104 72-73 0.3940972301238119 1104 74-75 0.30793233554398824 1104 76-77 0.44682179575348613 1104 78-79 0.525368231689292 1104 80-81 0.5216951756805912 1104 82-83 0.5765906247954362 1104 84-85 0.6008692413454995 1104 86-87 0.5773302448289002 1104 88-89 0.5314167474895015 1104 90-91 0.3144431955125597 1104 92-93 0.4309329145817955 1104 94-95 0.3893908265992181 1104 96-97 0.4505056319072054 1104 98-99 0.2520627060866403 1104 100-101 0.3510161221185619 1105 1 0.19394248449497553 1105 2 0.19486661072966882 1105 3 0.22640299389010465 1105 4 0.2288447712233932 1105 5 0.14960013102079728 1105 6 0.05954563024783255 1105 7 0.2035516467415377 1105 8 0.22021858559445207 1105 9 0.2594208606583024 1105 10-11 0.2873520508946541 1105 12-13 0.22596522195065916 1105 14-15 0.4058526047551041 1105 16-17 0.42888909177425205 1105 18-19 0.39487596471488473 1105 20-21 0.3184744477369392 1105 22-23 0.30965513016018775 1105 24-25 0.38052768028394013 1105 26-27 0.39306979314783064 1105 28-29 0.25497139195814356 1105 30-31 0.28073179272886506 1105 32-33 0.3498306384322234 1105 34-35 0.38033813870579536 1105 36-37 0.5355472522263938 1105 38-39 0.5597367461662017 1105 40-41 0.3859019157163459 1105 42-43 0.2831585667260512 1105 44-45 0.20042814692259725 1105 46-47 0.22375130506706853 1105 48-49 0.40357468110904193 1105 50-51 0.5707806643009619 1105 52-53 0.45694520653586324 1105 54-55 0.4416072688106354 1105 56-57 0.36070743145386075 1105 58-59 0.30231505718376184 1105 60-61 0.6507168886894235 1105 62-63 0.5256063905358346 1105 64-65 0.3672513324792206 1105 66-67 0.3606052449785224 1105 68-69 0.31351037363918266 1105 70-71 0.5184962677373548 1105 72-73 0.6401781139411398 1105 74-75 0.5304492802638308 1105 76-77 0.3546276378245352 1105 78-79 0.5448461789325094 1105 80-81 0.47204282421088095 1105 82-83 0.5083598133091343 1105 84-85 0.4276916878194399 1105 86-87 0.426605949700793 1105 88-89 0.5073231833096656 1105 90-91 0.6183199635739811 1105 92-93 0.44938443966129427 1105 94-95 0.5129608935902255 1105 96-97 0.6754441888320599 1105 98-99 0.5267094220828454 1105 100-101 0.46177123179691293 1106 1 0.1007290426739047 1106 2 0.13318267617850665 1106 3 0.1226527945656315 1106 4 0.10482456748789559 1106 5 0.030806887965056262 1106 6 -0.054037219356935395 1106 7 -0.033438419133908326 1106 8 0.058880699034411066 1106 9 -0.07191543130596756 1106 10-11 0.14764578786090254 1106 12-13 0.05295882907125815 1106 14-15 0.03069392176696084 1106 16-17 -0.004572775642358806 1106 18-19 0.0042403836918154525 1106 20-21 0.04688786636389608 1106 22-23 0.15879601580281388 1106 24-25 0.11716051528883753 1106 26-27 0.1856801878413492 1106 28-29 0.18122858575951284 1106 30-31 0.20515830775016042 1106 32-33 0.1664593730479993 1106 34-35 0.17300237689283904 1106 36-37 0.17189450962521136 1106 38-39 0.19297454181356954 1106 40-41 -0.08365580140236517 1106 42-43 0.08205829564477796 1106 44-45 0.10986235535076361 1106 46-47 0.21310478967301094 1106 48-49 0.42055091334844974 1106 50-51 0.39341812570449974 1106 52-53 0.17632223731570207 1106 54-55 0.29738125867563525 1106 56-57 0.35093690828148993 1106 58-59 0.40170289808038007 1106 60-61 0.1287238924853824 1106 62-63 0.2627258878861056 1106 64-65 0.2055841722471925 1106 66-67 0.05931051604944315 1106 68-69 0.17247562594771892 1106 70-71 -0.019894143150629873 1106 72-73 0.12634447791072745 1106 74-75 0.14872893922742492 1106 76-77 0.10111486759750221 1106 78-79 0.13261521519610397 1106 80-81 0.15347158202077793 1106 82-83 0.30200358889773327 1106 84-85 0.07625313463552885 1106 86-87 0.11805581001834753 1106 88-89 0.2357687009423728 1106 90-91 0.46071695165178994 1106 92-93 0.24553090224731733 1106 94-95 0.5037712588769097 1106 96-97 0.39706607911143976 1106 98-99 0.5536570551435815 1106 100-101 0.6892196093474929 1107 1 0.015939604771944005 1107 2 -0.00667760029902098 1107 3 0.04187220269429304 1107 4 0.15253413323531362 1107 5 0.1425524942541756 1107 6 0.06003906593537067 1107 7 0.06476072595818749 1107 8 0.03446229428943326 1107 9 0.07477277737282861 1107 10-11 0.11233101920961275 1107 12-13 0.05478449506327365 1107 14-15 0.2744227737366103 1107 16-17 0.2363672714010221 1107 18-19 0.13682730063133874 1107 20-21 0.05785749134533802 1107 22-23 0.10224530350981098 1107 24-25 0.20182003688425 1107 26-27 0.15491932761957372 1107 28-29 0.2520603236294363 1107 30-31 0.36707798235377 1107 32-33 0.2612093867233938 1107 34-35 0.21329499139501706 1107 36-37 0.25618479540200667 1107 38-39 0.30099848548655217 1107 40-41 0.24331619137702631 1107 42-43 0.2754011105892502 1107 44-45 0.3519169475503148 1107 46-47 0.4614578807042875 1107 48-49 0.5138181228634053 1107 50-51 0.7462981650896481 1107 52-53 0.7302003855883044 1107 54-55 0.6745331806555228 1107 56-57 0.5441087807173446 1107 58-59 0.5391621649226153 1107 60-61 0.5656851793796633 1107 62-63 0.4712718411396821 1107 64-65 0.3182040273660931 1107 66-67 0.6027133073940902 1107 68-69 0.7438065159428007 1107 70-71 0.8162159477321822 1107 72-73 0.8613454937976357 1107 74-75 0.8278464070120819 1107 76-77 0.7812462686618389 1107 78-79 0.8080920959544216 1107 80-81 0.9297762152465339 1107 82-83 0.791903328674401 1107 84-85 0.6075864080575855 1107 86-87 0.587002882310415 1107 88-89 0.5405845909765219 1107 90-91 0.6973980881948094 1107 92-93 0.6539135321440881 1107 94-95 0.7013886133511136 1107 96-97 0.6096726495553852 1107 98-99 0.7373235472860813 1107 100-101 0.6462524771962315 1108 1 -0.01545812832596738 1108 2 0.035406753519964695 1108 3 0.053256550817550874 1108 4 0.12395578841558574 1108 5 0.004530622636018222 1108 6 0.0920206117575475 1108 7 0.05598217609203715 1108 8 0.10664860174514956 1108 9 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sequence quality scores pass #Quality Count 11 1.0 12 4.0 13 25.0 14 111.0 15 381.0 16 999.0 17 1943.0 18 2911.0 19 3941.0 20 5170.0 21 6862.0 22 7449.0 23 5817.0 24 4862.0 25 4860.0 26 5304.0 27 6321.0 28 7414.0 29 8681.0 30 10212.0 31 12499.0 32 15360.0 33 19689.0 34 26535.0 35 41839.0 36 68655.0 37 146456.0 38 285808.0 39 96902.0 40 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.2444825738073 25.637282651125954 15.04613387924986 19.072100895816885 2 13.237487877126444 25.3696457047075 40.69057408157863 20.702292336587426 3 17.565139714148064 31.84192829612976 33.65459892404047 16.93833306568171 4 9.216565576465097 20.840098471977896 46.493755160117345 23.449580791439665 5 8.373658662658599 43.68986486571257 38.06832367490989 9.868152796718938 6 21.909043119769052 45.55986445169053 19.948335066789284 12.582757361751137 7 21.795375248256402 36.60732768841453 27.52845146971554 14.06884559361352 8 22.664570627770342 35.57980024063463 23.203301135800487 18.552327995794542 9 24.15153708822057 18.8070142862699 26.074606773149796 30.96684185235974 10-11 21.42357090253043 28.4451966542 32.32332863690141 17.80790380636816 12-13 23.793972591740385 28.89842533614199 29.98247306661113 17.325129005506493 14-15 21.583094852451325 30.18948409088913 29.99464280561414 18.232778251045406 16-17 17.481140041076006 32.775616359642136 31.1215982756609 18.62164532362096 18-19 16.95508111695621 33.15695575860758 32.75830271033888 17.129660414097327 20-21 18.411874152352585 31.207162832362474 31.96695602207616 18.414006993208783 22-23 18.429415801696525 30.710402601622754 31.844911365914676 19.015270230766046 24-25 19.399818583065997 31.702044641613732 31.495347270402817 17.402789504917454 26-27 18.644102883224452 29.67421483227716 34.41928389240947 17.26239839208892 28-29 17.057015039622534 31.300485007435107 33.99877022694333 17.643729725999034 30-31 18.442937945270373 30.855577898590507 32.05852550609066 18.64295865004847 32-33 18.514199396907504 31.00989450586124 32.82889524420102 17.647010853030235 34-35 19.39040899105336 30.54410024853869 32.75278241635814 17.312708344049813 36-37 19.48017649885391 28.819698416302934 33.539675231068216 18.160449853774942 38-39 17.92884842903725 32.09122536725638 33.02415505000258 16.955771153703804 40-41 17.40718064785668 31.36047645155503 33.67536154789043 17.556981352697857 42-43 17.750003450183737 31.27058348252764 31.15741745592234 19.821995611366287 44-45 18.812032735343305 30.049092978060592 31.88509257156622 19.253781715029877 46-47 19.607080529797667 31.29736945445695 31.97586376918145 17.11968624656393 48-49 17.845986443051906 31.194472299372357 32.18287948975071 18.77666176782503 50-51 16.12859106568017 30.889109309683477 32.92641228768552 20.05588733695083 52-53 17.91322850629627 32.14969029896156 31.06802633180229 18.869054862939883 54-55 17.681988924213414 30.406848218564864 31.48588426881993 20.425278588401792 56-57 18.901172937009683 30.108624330193873 31.049081686550178 19.941121046246263 58-59 19.903545408809133 29.743908543784087 30.726081758063078 19.6264642893437 60-61 19.43365476539976 31.69179658193005 31.555231865184215 17.31931678748598 62-63 17.387545965856983 31.282941413370907 32.77260529019809 18.556907330574024 64-65 17.235926073226697 32.409896883417666 30.956554031759254 19.397623011596384 66-67 18.237420316438307 30.70983452918793 29.76034396449949 21.292401189874273 68-69 17.651265464664473 30.866974715798957 30.521642688935195 20.960117130601375 70-71 19.292925617802446 30.365255269685182 31.30533624236098 19.036482870151396 72-73 19.02776331488635 29.285410490315023 32.32564966959786 19.36117652520077 74-75 17.352103169276052 32.356889515079814 30.24387153272217 20.04713578292197 76-77 17.49525442909496 31.102339977341703 31.361041027075785 20.041364566487548 78-79 18.555926921252908 30.901389861214618 30.601850806340313 19.940832411192158 80-81 19.192383074726273 30.21122713504533 30.397936162577388 20.198453627651006 82-83 18.852707381475014 30.465405001533846 31.75691062469927 18.92497699229187 84-85 17.696745159392215 31.196122244400982 31.059432237764078 20.047700358442725 86-87 16.914118026394533 31.775753112379384 31.416306697496672 19.89382216372941 88-89 18.29575978691665 30.55526629772702 29.651882733900504 21.497091181455826 90-91 18.21326903027254 29.84007457415323 31.184015236011387 20.762641159562843 92-93 19.729342495348526 31.3757199906155 30.44655414467436 18.448383369361615 94-95 18.687324275869162 29.582690867300915 30.955926725625076 20.774058131204843 96-97 18.218538401799616 29.605022965677573 31.846262321860742 20.330176310662072 98-99 18.115660195794792 31.03358722503604 29.74196389476814 21.108788684401027 100-101 17.22375633422369 29.77828491993692 30.92324407603452 22.07471466980487 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 724.0 1 483.5 2 458.5 3 659.5 4 483.5 5 422.5 6 792.0 7 1441.0 8 2125.5 9 2516.5 10 2028.5 11 1284.5 12 1126.5 13 1152.0 14 1174.5 15 1130.0 16 1081.0 17 1068.0 18 1066.5 19 1103.5 20 1188.0 21 1366.5 22 1504.0 23 1725.0 24 1950.0 25 2411.0 26 3590.0 27 4983.5 28 5741.5 29 7573.5 30 11839.5 31 16023.5 32 24493.0 33 35658.0 34 47131.0 35 55975.5 36 63883.5 37 68393.0 38 60945.0 39 53332.0 40 43994.0 41 33815.0 42 27428.5 43 21733.5 44 19354.0 45 18165.0 46 16041.0 47 21688.5 48 37185.5 49 33064.5 50 15783.5 51 7634.5 52 3068.0 53 1573.0 54 950.5 55 668.5 56 486.5 57 411.5 58 388.0 59 363.0 60 322.0 61 252.5 62 215.0 63 198.0 64 150.5 65 95.5 66 57.0 67 45.0 68 40.0 69 25.0 70 14.0 71 16.0 72 15.0 73 10.5 74 9.0 75 21.0 76 23.5 77 12.5 78 19.5 79 26.5 80 15.5 81 6.0 82 4.0 83 0.5 84 0.0 85 0.0 86 0.5 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04466419675331437 2 0.0015055347220218327 3 3.763836805054582E-4 4 0.010162359373647371 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0022583020830327493 24-25 0.0 26-27 0.0 28-29 0.02057564120096505 30-31 0.01775276359717411 32-33 6.273061341757635E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03412545369916154 50-51 0.012357930843262543 52-53 0.0 54-55 6.273061341757637E-4 56-57 0.0 58-59 0.0 60-61 1.254612268351527E-4 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.254612268351527E-4 80-81 0.012169739003009815 82-83 0.005457563367329144 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 797059.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 13.754750260730535 #Duplication Level Percentage of deduplicated Percentage of total 1 72.96463410923597 10.036103200381218 2 9.365579589565098 2.576424166029262 3 3.679455651802721 1.5182998075793976 4 2.0705438656241992 1.1391925510219385 5 1.4049957461193263 0.9662682802630049 6 1.021800726530032 0.8432768285792166 7 0.7516472187937006 0.7237103845076026 8 0.6257599873488036 0.6885737879312553 9 0.5467359553477378 0.6768194871933051 >10 5.314824804390838 16.03360715444375 >50 1.072337577210329 10.45284816472117 >100 1.0263933793864075 28.91794985961862 >500 0.10383388708206269 9.979516890439937 >1k 0.047781965736878415 11.578605617330766 >5k 0.002756651869435293 2.2130922864257365 >10k+ 9.18883956478431E-4 1.6557115335338175 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 13100 1.643542071540501 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 6729 0.8442285953737426 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5615 0.7044647886793826 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5166 0.648132697830399 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4919 0.6171437748021162 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3801 0.4768781232004155 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3556 0.4461401226258031 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3369 0.4226788732076296 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 3295 0.4133947424218283 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2927 0.367225010946492 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2823 0.35417704335563616 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2810 0.3525460474067792 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2729 0.3423836880331318 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 2439 0.3059999322509375 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 2267 0.2844206012352912 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2181 0.2736309357274681 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2138 0.26823610297355654 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 2090 0.2622139640854692 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 2089 0.26208850285863405 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1993 0.2500442250824594 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 1963 0.24628038827740484 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1944 0.24389662496753692 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 1889 0.23699625749160352 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1784 0.22382282867391248 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 1677 0.21039847740255113 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1545 0.19383759546031096 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 1478 0.18543169326235573 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 1444 0.18116601154996054 No Hit ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT 1385 0.17376379916668655 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 1366 0.17138003585681863 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 1277 0.16021398666849004 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 1250 0.1568265335439409 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 1240 0.1555719212755894 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 1237 0.15519553759508392 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 1228 0.15406638655356755 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1224 0.15356454164622693 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1216 0.15256085183154572 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 1207 0.15143170079002935 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 1202 0.1508043946558536 No Hit CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC 1175 0.14741694153130447 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 1171 0.14691509662396385 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1169 0.14666417417029354 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1154 0.14478225576776627 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 1143 0.14340218227257956 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1141 0.14315125981890928 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1121 0.14064203528220623 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 1118 0.14026565160170076 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1111 0.1393874230138547 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1078 0.13524720252829464 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 1075 0.13487081884778918 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 1051 0.1318597494037455 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 1046 0.13123244326956976 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 1041 0.13060513713539398 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 1014 0.12721768401084485 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 1012 0.12696676155717457 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1008 0.12646491664983395 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 978 0.12270107984477938 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 966 0.12119554512275753 No Hit GTGTAAGCATCTGGGTAGTCTGAGTAGCGTCGTGGTATTCCTGAAAGGCC 966 0.12119554512275753 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 963 0.12081916144225209 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 956 0.119940932854406 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 920 0.11542432868834052 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 906 0.11366787151264837 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 901 0.11304056537847261 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 901 0.11304056537847261 No Hit TAGTATAGCTGGAGATCATTTAATATTACCTCCGTGTAGGGTTGCAAGTC 892 0.11191141433695624 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 887 0.11128410820278048 No Hit GTTTATATTCTTATCCTCCCAGGATTTGGAATTATTTCACATGTAGTTAC 885 0.11103318574911018 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 881 0.11053134084176956 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 880 0.1104058796149344 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 877 0.11002949593442894 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 873 0.10952765102708834 No Hit CTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGTTTG 858 0.10764573262456105 No Hit ATACTATGTAGTAGCCCATTTCCACTATGTTCTATCAATGGGAGCAGTGT 850 0.10664204280987982 No Hit TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA 847 0.10626565912937437 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 844 0.1058892754488689 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 842 0.10563835299519861 No Hit ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA 842 0.10563835299519861 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 841 0.10551289176836344 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 840 0.10538743054152828 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 837 0.10501104686102282 No Hit ATTTTAGGGGGTTCGATTCCTTCCTTTCTTATTTTACTTTTACATAGGTT 836 0.10488558563418768 No Hit GTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAACAATTC 834 0.10463466318051738 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 832 0.10438374072684706 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 825 0.103505512139001 No Hit CATATTCACAGTAGGATTAGATGTAGACACACGAGCTTACTTTACATCAG 817 0.10250182232431977 No Hit ACTTACTACTCCGGAAAAAAAGAACCTTTCGGCTATATAGGAATAGTATG 816 0.10237636109748463 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 811 0.10174905496330887 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 807 0.10124721005596825 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 801 0.10049444269495733 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 800 0.10036898146812219 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.2546122683515274E-4 0.0 2 0.0 0.0 0.0 1.2546122683515274E-4 0.0 3 0.0 0.0 0.0 1.2546122683515274E-4 0.0 4 0.0 0.0 0.0 1.2546122683515274E-4 0.0 5 0.0 0.0 0.0 1.2546122683515274E-4 0.0 6 0.0 0.0 0.0 1.2546122683515274E-4 0.0 7 0.0 0.0 0.0 1.2546122683515274E-4 0.0 8 0.0 0.0 0.0 1.2546122683515274E-4 0.0 9 0.0 0.0 0.0 1.2546122683515274E-4 0.0 10-11 0.0 0.0 0.0 9.409592012636455E-4 0.0 12-13 0.0 0.0 0.0 0.0035129143513842765 0.0 14-15 0.0 0.0 0.0 0.0061476001149224834 0.0 16-17 0.0 0.0 0.0 0.01292250636402073 0.0 18-19 0.0 0.0 0.0 0.0228339432839978 0.0 20-21 0.0 0.0 0.0 0.03707379252978763 0.0 22-23 0.0 0.0 0.0 0.06009592765403816 0.0 24-25 0.0 0.0 0.0 0.09108485068232089 0.0 26-27 0.0 0.0 0.0 0.10576381422203376 0.0 28-29 0.0 0.0 0.0 0.1213210063495927 0.0 30-31 0.0 0.0 0.0 0.14490771699460142 0.0 32-33 0.0 0.0 0.0 0.17639848493022475 0.0 34-35 0.0 0.0 0.0 0.2277121267058022 0.0 36-37 0.0 0.0 0.0 0.27927669093505 0.0 38-39 0.0 0.0 0.0 0.34809217385413127 0.0 40-41 0.0 0.0 0.0 0.4364796081594964 0.0 42-43 0.0 0.0 0.0 0.5135755320496977 0.0 44-45 0.0 0.0 0.0 0.593933447837613 0.0 46-47 0.0 0.0 0.0 0.6929223558105485 0.0 48-49 0.0 0.0 0.0 0.7895275004736162 0.0 50-51 0.0 0.0 0.0 0.8938485105870456 0.0 52-53 0.0 0.0 0.0 0.9891363123683441 0.0 54-55 0.0 0.0 0.0 1.0890034489291258 0.0 56-57 0.0 0.0 0.0 1.2114536063202348 0.0 58-59 0.0 0.0 0.0 1.3401768250531014 0.0 60-61 0.0 0.0 0.0 1.4767413704631651 0.0 62-63 0.0 0.0 0.0 1.6146859893684158 0.0 64-65 0.0 0.0 0.0 1.7594055145227643 0.0 66-67 0.0 0.0 0.0 1.9230069543158035 0.0 68-69 0.0 0.0 0.0 2.1116379088624555 0.0 70-71 0.0 0.0 0.0 2.336778080418137 0.0 72-73 0.0 0.0 0.0 2.5611654846128076 0.0 74-75 0.0 0.0 0.0 2.786431117395325 0.0 76-77 0.0 0.0 0.0 3.0211690728038954 0.0 78-79 0.0 0.0 0.0 3.329552768364701 0.0 80-81 0.0 0.0 0.0 3.6796523218481942 0.0 82-83 0.0 0.0 0.0 4.017833058782348 0.0 84-85 0.0 0.0 0.0 4.329039631946944 0.0 86-87 0.0 0.0 0.0 4.659755425884407 0.0 88-89 0.0 0.0 0.0 5.029991506274943 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGCGT 15 6.619609E-4 94.99122 6 CGCGTAC 15 6.619609E-4 94.99122 8 CATAGGC 30 1.3661829E-6 79.17424 4 GACATAT 320 0.0 72.76873 1 GCGTACG 20 0.0020746035 71.243416 9 ACATATA 380 0.0 61.255863 2 TGGACTG 65 6.712071E-10 58.456135 5 GACTGGT 60 2.3517714E-8 55.41154 7 GCCTATG 35 2.7886961E-4 54.2807 9 TTGGACT 65 4.4137778E-8 51.158745 4 TGGGACC 40 5.389636E-4 47.49561 6 TAACTTT 950 0.0 44.995842 6 GGGAGCC 170 0.0 44.701748 8 TGGGGAG 210 0.0 42.972218 6 GGCCAGC 45 9.627677E-4 42.218315 8 TGGGAGT 45 9.627677E-4 42.218315 6 GACTGAC 45 9.627677E-4 42.218315 7 TACAATC 45 9.627677E-4 42.218315 7 TACCTGG 105 1.02591E-9 40.71818 2 GGAACAC 70 4.311267E-6 40.710526 8 >>END_MODULE