FastQCFastQC Report
Fri 10 Feb 2017
SRR2031599_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031599_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209587
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG34550.027.7336351
CCGCGTA350.00836141827.16408710-11
TACATGG36200.025.9083212
GCACCGT2600.025.5979926
ACATGGG36150.025.3931313
CATGGGG25200.023.781394
TTACGCC1050.002179490222.6489434
GTATCAA32250.022.6162471
ATGGGGG15650.020.9701735
GTATAAG4450.020.3541241
TCAACGC34350.020.0774484
CAACGCA34600.019.9323795
AACGCAG35150.019.7459816
GTCCTAC3153.0559022E-1019.6739521
ATCAACG35500.019.4270523
TATCAAC36750.019.3023662
GTGTAGG6400.018.621681
GTGTAGC7700.018.5733131
TACCTGG11300.018.53772
GTACCGT1559.571824E-418.4022436
Produced by FastQC (version 0.11.5)