FastQCFastQC Report
Fri 10 Feb 2017
SRR2031599_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031599_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209587
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG33150.025.0887781
TACATGG32850.023.8583322
ACATGGG33500.021.125023
GTATCAA28950.020.5204281
GGTATCA23500.020.2235371
TAGACCG1908.258077E-619.9982515
CCTATAC2953.0195224E-919.3203453
GTCTAGA5800.018.8462451
CATGGGG21950.018.6088524
AACGCAG31550.018.5166056
CTATACT5150.018.444994
TCAACGC31200.018.4198634
ATCAACG31600.018.3370043
TATCACG1300.00756355718.2691442
CAACGCA31300.018.2092695
TATATAC5750.018.1723233
GTACGGA1851.5152269E-417.982551
GAGTACA27700.017.843441
TCGATCG800.001192230517.81094240-41
GTATAAG4550.017.756711
Produced by FastQC (version 0.11.5)