##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031597_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1953800 Sequences flagged as poor quality 0 Sequence length 101 %GC 40 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.48679752277613 34.0 31.0 34.0 31.0 34.0 2 32.6847353874501 34.0 31.0 34.0 31.0 34.0 3 32.797793018732726 34.0 31.0 34.0 31.0 34.0 4 36.20323267478759 37.0 35.0 37.0 35.0 37.0 5 35.95311290817893 37.0 35.0 37.0 35.0 37.0 6 36.06001279557785 37.0 37.0 37.0 35.0 37.0 7 36.01601494523493 37.0 37.0 37.0 35.0 37.0 8 36.12598270037875 37.0 37.0 37.0 35.0 37.0 9 37.98181594840823 39.0 39.0 39.0 35.0 39.0 10-11 37.95102722898966 39.0 38.0 39.0 35.0 39.0 12-13 37.99815231855871 39.0 39.0 39.0 35.0 39.0 14-15 39.322198536185894 41.0 39.0 41.0 36.0 41.0 16-17 39.362343638038695 41.0 39.0 41.0 36.0 41.0 18-19 39.46225662810933 41.0 39.5 41.0 36.5 41.0 20-21 39.495741375780526 41.0 39.0 41.0 37.0 41.0 22-23 39.425185791790355 41.0 39.0 41.0 36.5 41.0 24-25 39.31132869280377 41.0 39.0 41.0 36.0 41.0 26-27 39.17344994369945 41.0 39.0 41.0 36.0 41.0 28-29 39.158914934998464 41.0 39.0 41.0 36.0 41.0 30-31 39.083130310164805 40.0 39.0 41.0 36.0 41.0 32-33 38.91836395741632 40.0 38.0 41.0 35.0 41.0 34-35 38.75952272494626 40.0 38.0 41.0 35.0 41.0 36-37 38.60330765687378 40.0 38.0 41.0 34.5 41.0 38-39 38.48742783294094 40.0 38.0 41.0 34.0 41.0 40-41 38.51842614392466 40.0 38.0 41.0 34.5 41.0 42-43 38.39818251612243 40.0 38.0 41.0 34.0 41.0 44-45 38.312095659739995 40.0 38.0 41.0 34.0 41.0 46-47 38.31436303613471 40.0 38.0 41.0 33.5 41.0 48-49 38.387468011055375 40.0 38.0 41.0 34.0 41.0 50-51 38.30286774490736 40.0 38.0 41.0 34.0 41.0 52-53 38.35975816357867 40.0 38.0 41.0 34.0 41.0 54-55 38.31375959668338 40.0 38.0 41.0 34.0 41.0 56-57 38.200667929163686 40.0 38.0 41.0 34.0 41.0 58-59 38.05627469546525 40.0 38.0 41.0 33.5 41.0 60-61 37.699939348960996 40.0 38.0 41.0 32.5 41.0 62-63 37.624101750435045 40.0 37.0 41.0 33.0 41.0 64-65 37.38652497696796 39.5 37.0 41.0 32.0 41.0 66-67 37.080541764766096 39.0 36.5 41.0 32.0 41.0 68-69 36.733103695362885 39.0 36.0 41.0 31.5 41.0 70-71 36.27478477838059 37.5 35.0 40.0 31.0 41.0 72-73 35.87281860988843 37.0 35.0 39.5 30.5 41.0 74-75 35.54088801310267 37.0 35.0 39.0 31.0 41.0 76-77 34.88315871634763 36.0 34.0 39.0 30.0 40.0 78-79 34.7929429829051 36.0 34.0 38.0 30.0 39.5 80-81 34.419272443443546 35.5 34.0 37.0 30.0 39.0 82-83 34.054215375166336 35.0 34.0 37.0 29.5 39.0 84-85 33.702049851571296 35.0 34.0 36.0 29.0 38.0 86-87 33.56226532910226 35.0 34.0 36.0 29.0 37.0 88-89 33.311597911761694 35.0 34.0 36.0 29.0 37.0 90-91 33.09433335039411 35.0 34.0 35.0 29.0 36.5 92-93 32.95641723820248 35.0 34.0 35.0 29.0 36.0 94-95 32.77841949022418 35.0 34.0 35.0 29.0 36.0 96-97 32.63248566895281 35.0 34.0 35.0 28.0 36.0 98-99 32.653062749513765 35.0 34.0 35.0 29.0 35.5 100-101 32.197338263896 34.5 33.0 35.0 26.5 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18180191588498218 1101 2 0.1939424115671997 1101 3 0.20810498393110777 1101 4 0.08879406761268172 1101 5 0.152814500597529 1101 6 0.11505992203786519 1101 7 0.1922206098693735 1101 8 0.13677528304204856 1101 9 0.14335342730898049 1101 10-11 0.08885961054426161 1101 12-13 0.12490347621616849 1101 14-15 0.17611921716438417 1101 16-17 0.16642151788853 1101 18-19 0.15020300392014718 1101 20-21 0.16491658832631373 1101 22-23 0.15484086079226955 1101 24-25 0.16697178449202 1101 26-27 0.2680882863432785 1101 28-29 0.19429906984775158 1101 30-31 0.19132677749308868 1101 32-33 0.17842762023080638 1101 34-35 0.21070501769049343 1101 36-37 0.20191603967738558 1101 38-39 0.27903778094622567 1101 40-41 0.17355692985588433 1101 42-43 0.164080709076174 1101 44-45 0.2893920098927154 1101 46-47 0.2510746234462502 1101 48-49 0.30046288986641656 1101 50-51 0.4083646899219673 1101 52-53 0.2403827667467553 1101 54-55 0.3048031633787005 1101 56-57 0.28800444332897257 1101 58-59 0.40962631413646733 1101 60-61 0.17073033039597618 1101 62-63 0.1889378366426726 1101 64-65 0.3098804104864641 1101 66-67 0.36710844071558313 1101 68-69 0.32576438709560307 1101 70-71 0.30534019087857445 1101 72-73 0.11780776580539509 1101 74-75 0.1093137452412023 1101 76-77 0.16511653131403392 1101 78-79 0.17054744894889495 1101 80-81 0.29182544903074614 1101 82-83 0.30663182259205257 1101 84-85 0.3294338119721232 1101 86-87 0.11198211778738454 1101 88-89 0.22256061030362417 1101 90-91 0.2064322451244962 1101 92-93 0.014932470372237105 1101 94-95 0.22677115338995435 1101 96-97 0.19079256534897837 1101 98-99 0.1973295578398364 1101 100-101 0.17361628032968213 1102 1 0.12074720892511692 1102 2 0.15767513006893807 1102 3 0.14265711713488827 1102 4 0.2173793185452908 1102 5 0.030943836552658865 1102 6 0.07720367702617636 1102 7 0.21162825886408854 1102 8 0.10864698072390411 1102 9 0.21055766627450367 1102 10-11 0.2492555462025905 1102 12-13 0.15445738687020594 1102 14-15 0.2179065254804584 1102 16-17 0.2314492416537064 1102 18-19 0.24473842868616913 1102 20-21 0.1759531965053398 1102 22-23 0.1696137626409424 1102 24-25 0.2501635500939656 1102 26-27 0.17171764925404176 1102 28-29 -0.17340553048536123 1102 30-31 -0.04293549147384823 1102 32-33 0.2591720982785901 1102 34-35 0.2789378267874696 1102 36-37 0.3170135440723669 1102 38-39 0.23733032615365346 1102 40-41 0.17822856365491901 1102 42-43 0.25250389383736405 1102 44-45 0.35328131426313547 1102 46-47 0.35246792555970075 1102 48-49 0.33099571554212304 1102 50-51 0.4554055088543052 1102 52-53 0.25683502467606445 1102 54-55 0.11223456737794635 1102 56-57 -0.11628827131385577 1102 58-59 0.1483581510066685 1102 60-61 0.4817914215842478 1102 62-63 0.21003213797905573 1102 64-65 0.36513418457882096 1102 66-67 0.4056420219596504 1102 68-69 0.32603232201457644 1102 70-71 0.40307076995443936 1102 72-73 0.4080345862934607 1102 74-75 0.367282174319719 1102 76-77 0.38638841553696324 1102 78-79 0.23925958186083562 1102 80-81 0.11621012088343008 1102 82-83 -0.052377458657332454 1102 84-85 0.09949174023122964 1102 86-87 0.017111470361768966 1102 88-89 0.17914441171237172 1102 90-91 0.3175261289526148 1102 92-93 0.30919878471055995 1102 94-95 0.3434564052548552 1102 96-97 0.3373817457234978 1102 98-99 0.1870006869808165 1102 100-101 0.18726717937091308 1103 1 0.14695270040219555 1103 2 0.12084720525338355 1103 3 0.1874689924627262 1103 4 0.16006582206679099 1103 5 0.05058775682398675 1103 6 0.05013036484123745 1103 7 0.1473125460754119 1103 8 0.0965671473762697 1103 9 0.21666589256057733 1103 10-11 0.18916810220203928 1103 12-13 0.08997850069788171 1103 14-15 0.2298743695482841 1103 16-17 0.2285806227817062 1103 18-19 0.25429869565937935 1103 20-21 0.1865633148714707 1103 22-23 0.12962339140995738 1103 24-25 0.1993806449688904 1103 26-27 0.09254526927403361 1103 28-29 -0.011710905624440215 1103 30-31 0.10292804400847189 1103 32-33 0.24876941022800025 1103 34-35 0.16478361331589042 1103 36-37 0.26750158257820544 1103 38-39 0.18808044266484103 1103 40-41 0.07894006118250019 1103 42-43 0.17539581109767965 1103 44-45 0.1923646492961879 1103 46-47 0.18149704777540165 1103 48-49 0.10181446493358948 1103 50-51 0.1828126337585232 1103 52-53 0.17077472672301042 1103 54-55 0.08074806939818302 1103 56-57 0.10423805521071472 1103 58-59 0.17658483732083852 1103 60-61 0.12000678197228609 1103 62-63 0.1288356768403034 1103 64-65 0.1761559308957814 1103 66-67 0.19652590216113452 1103 68-69 0.07319285499118422 1103 70-71 0.17567769181555803 1103 72-73 0.13236431506003754 1103 74-75 0.06684303010253956 1103 76-77 0.036533712968555676 1103 78-79 0.0941665018362201 1103 80-81 0.17561697024014933 1103 82-83 0.10326262550010767 1103 84-85 0.07150115502759746 1103 86-87 0.07472341249832937 1103 88-89 0.15639885020159028 1103 90-91 0.1265803660150695 1103 92-93 0.060044765584642334 1103 94-95 0.18010637871994106 1103 96-97 0.1796937201483786 1103 98-99 0.21286116681062595 1103 100-101 0.20280694268484467 1104 1 0.10763413205866357 1104 2 0.11035176364558197 1104 3 0.04770293037094575 1104 4 0.12683209518503702 1104 5 0.13782403808181698 1104 6 0.16062183591627388 1104 7 0.1635159961742758 1104 8 0.19083458040477552 1104 9 0.27189416510677233 1104 10-11 0.14118605195127287 1104 12-13 0.1907101816523351 1104 14-15 0.1710250078849498 1104 16-17 0.27641837357784027 1104 18-19 0.2803393245035366 1104 20-21 0.13046005454937415 1104 22-23 0.2004928057343136 1104 24-25 0.18992544618122764 1104 26-27 0.1958574288835635 1104 28-29 -0.0024505570557735723 1104 30-31 0.10189166142852457 1104 32-33 0.10297732938206394 1104 34-35 0.1273089530354099 1104 36-37 0.1323222211133981 1104 38-39 0.07127435392636983 1104 40-41 -0.09115627570691487 1104 42-43 0.043296002197955374 1104 44-45 -0.03797294157569553 1104 46-47 0.12381329482783343 1104 48-49 0.1449041202986976 1104 50-51 0.18491223677877855 1104 52-53 0.06999617142341208 1104 54-55 0.016536282651081535 1104 56-57 0.028261726938829668 1104 58-59 -0.05407664608490137 1104 60-61 0.11684083355538633 1104 62-63 0.008255559944878144 1104 64-65 -0.12606006755718369 1104 66-67 -0.1605012790767688 1104 68-69 -0.07785319657133272 1104 70-71 -0.02547556413160379 1104 72-73 -0.030536901761195168 1104 74-75 -0.3036848816398745 1104 76-77 -0.1201036207745787 1104 78-79 -0.0017909811107088558 1104 80-81 0.10786313738928044 1104 82-83 0.12226640412379197 1104 84-85 0.037531225195735374 1104 86-87 -0.03342250311020223 1104 88-89 -0.015458675318278381 1104 90-91 -0.15621016852893632 1104 92-93 -0.08019996629042936 1104 94-95 -0.06633300925919627 1104 96-97 -0.10032258844730535 1104 98-99 -0.055500709016108374 1104 100-101 0.08121323700866867 1105 1 0.12438889128470976 1105 2 0.09455567239320573 1105 3 0.02985095928832493 1105 4 0.08194109232226054 1105 5 -0.008163619677191036 1105 6 0.07166900488689976 1105 7 0.08793021314374272 1105 8 0.09594664082383275 1105 9 0.14641100201764345 1105 10-11 0.11949209886960688 1105 12-13 0.11017162885806897 1105 14-15 0.12200135934920553 1105 16-17 0.12569724918631664 1105 18-19 0.05182546176083491 1105 20-21 0.10409738147394876 1105 22-23 0.09159069986728952 1105 24-25 0.09653294509515575 1105 26-27 0.12241246213410051 1105 28-29 0.08367395978113734 1105 30-31 -0.004628046612872083 1105 32-33 0.12138521649735168 1105 34-35 0.1105092888824899 1105 36-37 0.17214662544432713 1105 38-39 0.15685548633812374 1105 40-41 0.13855157075025915 1105 42-43 0.20422864620005043 1105 44-45 0.05959606580959331 1105 46-47 0.2204205013375926 1105 48-49 0.20467153366352164 1105 50-51 0.10900550535618692 1105 52-53 0.18604686316814423 1105 54-55 0.14248450761849796 1105 56-57 0.024030917007117125 1105 58-59 0.09216477672595857 1105 60-61 0.2003023837987854 1105 62-63 0.06541500654908816 1105 64-65 0.13540356850266022 1105 66-67 0.10926942035401765 1105 68-69 0.025962469051961534 1105 70-71 0.004792097080560609 1105 72-73 0.195128805077168 1105 74-75 0.15085914885927565 1105 76-77 0.06687979851630388 1105 78-79 0.11791511092141604 1105 80-81 0.1324042020746603 1105 82-83 0.20446609111208147 1105 84-85 0.25015605393043217 1105 86-87 0.14282202475452266 1105 88-89 0.17754165607312444 1105 90-91 -0.0274412875736445 1105 92-93 -0.08132154516158607 1105 94-95 -0.08668225123618356 1105 96-97 0.11765045188776213 1105 98-99 0.0886674289541034 1105 100-101 0.23822139926235053 1106 1 0.1497943299814395 1106 2 0.09879646419112476 1106 3 0.12156934319447998 1106 4 0.12761387944466662 1106 5 0.15008458113945267 1106 6 0.027928079427866237 1106 7 0.09970582235010994 1106 8 0.20577872577987932 1106 9 0.15560216885291567 1106 10-11 0.12810161056102487 1106 12-13 0.0660094088256784 1106 14-15 0.13770391882839306 1106 16-17 0.08086017159472902 1106 18-19 0.043376638240680165 1106 20-21 0.06013155809531412 1106 22-23 0.02169232817409039 1106 24-25 0.021509095760059438 1106 26-27 0.18636471628938267 1106 28-29 0.1516476770276114 1106 30-31 0.11491527138001345 1106 32-33 0.0375638126363711 1106 34-35 -0.0656722687673792 1106 36-37 0.012420781413737814 1106 38-39 0.028782591238510236 1106 40-41 0.07028317662295791 1106 42-43 0.09971532333246813 1106 44-45 -0.023588645980360923 1106 46-47 0.04459471810969973 1106 48-49 0.14505736873095287 1106 50-51 0.27103618451187117 1106 52-53 0.08547359460366977 1106 54-55 0.23287610845925855 1106 56-57 0.12108231363465904 1106 58-59 0.06902813707107214 1106 60-61 0.18424290329495818 1106 62-63 0.0615774912828968 1106 64-65 0.26258932075261754 1106 66-67 0.2540591268761503 1106 68-69 0.0884957205287904 1106 70-71 0.1315947202453458 1106 72-73 0.2694394675645171 1106 74-75 0.24242601741537584 1106 76-77 0.15411431086292282 1106 78-79 0.1989012940318986 1106 80-81 0.2704361601836851 1106 82-83 0.19127131542687437 1106 84-85 0.17143375585043685 1106 86-87 0.16988184727353683 1106 88-89 0.198098112166754 1106 90-91 0.24055988261626027 1106 92-93 0.09871833040254785 1106 94-95 0.23386362335300248 1106 96-97 0.16176598881229864 1106 98-99 0.2566576572787298 1106 100-101 0.37958325526478376 1107 1 0.14772668226027008 1107 2 0.1892600188486142 1107 3 0.11510336692360568 1107 4 0.13565652864577515 1107 5 0.10871728500384137 1107 6 0.09098157035322885 1107 7 0.13841746055813786 1107 8 0.06576730123285301 1107 9 0.05141237490365569 1107 10-11 0.10346270131233126 1107 12-13 0.048386038059462066 1107 14-15 0.09827077043194521 1107 16-17 0.1549543398883202 1107 18-19 0.15589911963764536 1107 20-21 0.08720230301605625 1107 22-23 0.08863105000407501 1107 24-25 0.18392349557159804 1107 26-27 0.10029247189684298 1107 28-29 0.05829686104520704 1107 30-31 0.11685552197550209 1107 32-33 0.14549938722334588 1107 34-35 -0.0013437385853976025 1107 36-37 0.006445322219569505 1107 38-39 0.018009676691313814 1107 40-41 0.11563136593600376 1107 42-43 0.12486916108314716 1107 44-45 0.09908624204747696 1107 46-47 0.22865305043097806 1107 48-49 0.05472441519871296 1107 50-51 0.09052620542636447 1107 52-53 0.018143221969367573 1107 54-55 0.24281705111864937 1107 56-57 0.12557172731494148 1107 58-59 0.17560796212659113 1107 60-61 0.22875576414922705 1107 62-63 0.166549671412362 1107 64-65 -0.021500776935802435 1107 66-67 0.27711617576674286 1107 68-69 0.24323089623307226 1107 70-71 0.23273217060623352 1107 72-73 0.30927220047851023 1107 74-75 0.2257701019472549 1107 76-77 0.20777031848417238 1107 78-79 0.21906757245518804 1107 80-81 0.2986441723796389 1107 82-83 0.2935225370661385 1107 84-85 0.36871270581738713 1107 86-87 0.22600112027688368 1107 88-89 0.10251883819916685 1107 90-91 0.1947046985144496 1107 92-93 0.3477072396817533 1107 94-95 0.37831487587271795 1107 96-97 0.14507162534936668 1107 98-99 0.308106414340493 1107 100-101 0.3758217597720517 1108 1 0.17400973719141177 1108 2 0.14501698060910684 1108 3 0.1620189980387039 1108 4 0.13974927404278503 1108 5 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-1.359545266639941 2316 86-87 -0.8874635550520011 2316 88-89 -0.6705148617359455 2316 90-91 -0.6547172300868382 2316 92-93 -0.7166285282992106 2316 94-95 -0.7541445970626626 2316 96-97 -0.5948390069048131 2316 98-99 -0.7464768897585188 2316 100-101 -0.8954964912838079 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 1.0 11 0.0 12 8.0 13 35.0 14 187.0 15 484.0 16 1029.0 17 2063.0 18 3286.0 19 4435.0 20 6357.0 21 8422.0 22 9753.0 23 8708.0 24 8072.0 25 8335.0 26 9557.0 27 11936.0 28 14787.0 29 18427.0 30 22953.0 31 28815.0 32 37123.0 33 47834.0 34 66594.0 35 100200.0 36 178736.0 37 377392.0 38 702873.0 39 275305.0 40 92.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.54130861565214 24.757160252215385 15.423409086021339 19.278122046111136 2 12.567446802630004 24.95897657954244 40.113022978233786 22.360553639593768 3 19.794819194907944 30.1362634347926 32.458213246159126 17.610704124140327 4 9.989040582604881 19.18251243492046 46.253962615128906 24.574484367345747 5 9.168901627597501 43.768348858634454 36.61423891902958 10.448510594738458 6 23.548162555020983 43.89845429419593 19.294195925888012 13.259187224895078 7 22.956290306070223 34.787644590029686 26.941754529634558 15.314310574265534 8 22.916112191626574 34.312928651857916 22.598116490940733 20.172842665574777 9 23.634507114341282 19.87967038591463 25.400194492783296 31.085628006960796 10-11 22.427423482444468 27.42560651039001 31.392849831098374 18.75412017606715 12-13 25.344917596478655 27.18678984542942 29.061597911761694 18.40669464633023 14-15 21.74587982393285 28.757549390930492 30.31507830893643 19.181492476200226 16-17 18.267888217831917 32.12112293991197 30.213711741222234 19.397277101033882 18-19 17.81134200020473 33.308885249257855 30.365006653700483 18.514766096836933 20-21 20.62959361244754 29.83199406285188 28.834143719930395 20.70426860477019 22-23 19.767539763844315 29.73911499070891 29.838180712152273 20.655164533294503 24-25 21.480729859760466 30.383253147712153 28.78040229296755 19.35561469955983 26-27 20.372939911966427 27.97517657897431 32.77057528918006 18.88130821987921 28-29 18.26185648180618 31.755779588539472 30.72853608329754 19.253827846356806 30-31 20.14524974366036 29.293874519251194 29.386530484580458 21.17434525250798 32-33 20.23641109632511 29.201453577643566 30.856510390009213 19.70562493602211 34-35 21.52182925580919 28.967140956085576 30.615160200634662 18.89586958747057 36-37 21.183002354386325 27.514663732214146 31.864469239430854 19.437864673968676 38-39 19.44091002149657 31.01335858327362 31.28866823625755 18.257063158972258 40-41 19.25481113727096 30.33762411710513 31.200864981062544 19.206699764561368 42-43 18.802436278022316 30.4226635274849 28.199636605589106 22.575263588903674 44-45 20.697640495444773 28.57626164397584 29.0435305558399 21.682567304739482 46-47 22.111603029992835 29.927372300133076 29.54542430136145 18.415600368512642 48-49 19.977082504519757 29.38188299277095 29.98758891665681 20.65344558605248 50-51 17.149456824280833 30.13466457987543 31.81232187944302 20.903556716400722 52-53 19.371737127648682 31.05496980243628 29.07554509161634 20.4977479782987 54-55 18.8909572398996 28.978024182816526 29.937572423563708 22.193446153720163 56-57 21.22320605998567 28.333990172996216 28.998413348346812 21.444390418671308 58-59 22.55921793428191 28.129107380489305 28.516787798136967 20.79488688709182 60-61 21.016639629603752 29.716150505719753 31.177968875516655 18.089240989159837 62-63 18.537209540382843 30.095557375371072 31.41595864469239 19.95127443955369 64-65 18.536979219981575 32.2582147609786 28.93699457467499 20.267811444364828 66-67 20.264817279148325 29.60924864366875 27.709438018220904 22.416496058962025 68-69 19.531246801105535 29.519193366772445 28.911454601289794 22.038105230832226 70-71 21.45736513460948 29.025898249564953 29.115134609478964 20.401602006346607 72-73 20.125166342512028 28.03216808271062 31.467934281912168 20.374731292865185 74-75 18.684665779506602 31.9820094175453 28.123144641211994 21.210180161736105 76-77 19.025795884942166 29.865288156413143 29.380156617872867 21.72875934077183 78-79 20.08679011195653 29.599563720637896 29.001752487723888 21.31189367968169 80-81 21.213597140623143 28.98443748568328 28.555530956747187 21.24643441694639 82-83 20.32944702814867 28.984392674814636 30.763147630800663 19.92301266623603 84-85 19.798316101955166 29.82482853925683 29.8926451018528 20.484210256935203 86-87 18.138269014228683 31.145127443955367 30.01230934589006 20.704294195925886 88-89 19.157641519091 29.96959770703245 27.6080970416624 23.264663732214146 90-91 19.894206162350294 28.408895485720137 29.026154161121916 22.670744190807657 92-93 21.69684716961818 30.090874193878598 29.001816972054456 19.210461664448765 94-95 20.442317535059885 28.416035418159485 29.22254068993756 21.919106356843074 96-97 18.312058552564235 28.924326952605178 31.555200122837547 21.20841437199304 98-99 19.575928958951785 30.36247312928652 28.22620022520217 21.835397686559524 100-101 18.533089364315693 28.772520217012996 29.553178421537517 23.14121199713379 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 56.0 1 36.5 2 54.0 3 101.5 4 79.5 5 64.0 6 113.0 7 237.0 8 408.0 9 524.5 10 487.5 11 415.5 12 461.0 13 531.5 14 589.5 15 669.5 16 737.5 17 821.5 18 949.5 19 1051.0 20 1151.5 21 1299.0 22 1474.0 23 1694.0 24 1968.5 25 2378.5 26 3122.0 27 4123.0 28 5168.0 29 7335.0 30 12793.5 31 21071.0 32 39886.5 33 65144.0 34 87784.5 35 113002.0 36 151781.0 37 176662.5 38 158475.0 39 132727.0 40 107494.0 41 79957.0 42 62279.0 43 49646.0 44 41953.5 45 39131.0 46 38605.0 47 71876.0 48 142193.0 49 129057.5 50 56627.5 51 27802.5 52 18892.5 53 15234.5 54 13365.0 55 11965.0 56 10835.5 57 9315.0 58 7473.0 59 5981.5 60 4678.5 61 3584.5 62 2575.5 63 1792.5 64 1258.0 65 868.0 66 622.5 67 421.0 68 253.0 69 171.5 70 136.0 71 101.0 72 61.5 73 43.0 74 41.5 75 36.0 76 26.5 77 16.0 78 6.5 79 4.0 80 6.0 81 5.5 82 2.5 83 2.0 84 1.5 85 0.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.044323881666496055 2 0.0019449278329409356 3 5.630054253250076E-4 4 0.011720749309038795 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0023799774797829873 24-25 0.0 26-27 0.0 28-29 0.02134302385095711 30-31 0.01724843893950251 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03618589415498004 50-51 0.01241171051284676 52-53 0.0 54-55 8.189169822909203E-4 56-57 0.0 58-59 0.0 60-61 2.559115569659126E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.070938683590951E-4 80-81 0.011643975841949024 82-83 0.005220595762104617 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1953800.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.087585102279704 #Duplication Level Percentage of deduplicated Percentage of total 1 64.03618227596404 10.301875319896666 2 14.172046012102093 4.559879925862323 3 6.321052795692088 3.0507142436009853 4 3.5563633322860526 2.288531910511156 5 2.226733855675766 1.791138520165565 6 1.5235004142079167 1.4705665540156942 7 1.129974899752912 1.2724997154250464 8 0.8570581929820442 1.1030397293763758 9 0.6758111428068955 0.9784952345677351 >10 4.335169469925707 13.343981714805697 >50 0.526512864839906 5.977593032762408 >100 0.5037632827187866 17.14501703748313 >500 0.08011418697604046 8.894136041681683 >1k 0.0497828898493091 15.401895575838312 >5k 0.002637504097976641 2.83057678869151 >10k+ 0.003296880122470801 9.59005865531572 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 49624 2.5398710205752892 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 25916 1.3264407820657182 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 18595 0.9517350803562289 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 14567 0.7455727300644898 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 14121 0.7227454191831303 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 13767 0.7046268809499436 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 11605 0.5939707237178831 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 11123 0.5693008496263691 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 10852 0.5554304432388166 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 10642 0.5446821578462484 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 8956 0.45838878083734264 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 8422 0.43105742655338314 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 8192 0.4192854949329512 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 6834 0.34977991606100933 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 5309 0.27172689118640597 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 5278 0.2701402395332173 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 5273 0.2698843279762514 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 5104 0.26123451735080355 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 4717 0.24142696284164192 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 4492 0.22991094277817586 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 4405 0.225458081686969 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 4343 0.22228477838059169 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 4261 0.21808782884635067 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 4242 0.21711536492988026 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 4156 0.21271368615006656 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4148 0.21230422765892107 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 4052 0.20739072576517556 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 3983 0.20385914627904597 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3779 0.19341795475483675 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 3600 0.18425632101545705 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 3553 0.1818507523799775 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 3486 0.17842153751663425 No Hit CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT 3477 0.1779608967140956 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 3469 0.17755143822295014 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 3337 0.17079537311905008 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 3203 0.16393694339236362 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 3142 0.16081482239737946 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 3127 0.16004708772648174 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 3120 0.15968881154672945 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 3090 0.15815334220493396 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 3011 0.15410993960487254 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 2892 0.14801924454908383 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 2799 0.14325928958951786 No Hit TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA 2757 0.1411096325110042 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 2655 0.13588903674889957 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 2630 0.13460947896407002 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 2594 0.13276691575391544 No Hit ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG 2585 0.13230627495137678 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 2569 0.1314873579690859 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 2569 0.1314873579690859 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 2522 0.1290817893336063 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 2473 0.12657385607534036 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 2438 0.12478247517657898 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 2437 0.12473129286518579 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 2408 0.12324700583478351 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2398 0.12273518272085168 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 2307 0.11807759238407207 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 2285 0.11695158153342206 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 2231 0.1141877367181902 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 2195 0.11234517350803563 No Hit CATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCC 2193 0.11224280888524926 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 2189 0.11203807963967652 No Hit AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA 2186 0.11188453270549698 No Hit CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC 2119 0.10845531784215376 No Hit ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT 2117 0.10835295321936739 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 2114 0.10819940628518784 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 2107 0.10784113010543556 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 2018 0.10328590439144232 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 2018 0.10328590439144232 No Hit CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC 2011 0.10292762821169003 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 1983 0.10149452349268093 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 1958 0.10021496570785136 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 5.118231139318252E-5 0.0 3 0.0 0.0 0.0 5.118231139318252E-5 0.0 4 0.0 0.0 0.0 5.118231139318252E-5 0.0 5 0.0 0.0 0.0 5.118231139318252E-5 0.0 6 0.0 0.0 0.0 5.118231139318252E-5 0.0 7 0.0 0.0 0.0 1.0236462278636504E-4 0.0 8 0.0 0.0 0.0 1.0236462278636504E-4 0.0 9 0.0 0.0 0.0 1.5354693417954756E-4 0.0 10-11 0.0 0.0 0.0 6.909612038079639E-4 0.0 12-13 0.0 0.0 0.0 0.0012283754734363802 0.0 14-15 0.0 0.0 0.0 0.0020728836114238917 0.0 16-17 0.0 0.0 0.0 0.004862319582352339 0.0 18-19 0.0 0.0 0.0 0.007907667110246699 0.0 20-21 0.0 0.0 0.0 0.013179445183744497 0.0 22-23 0.0 0.0 0.0 0.021291841539563927 0.0 24-25 0.0 0.0 0.0 0.030427884123247005 0.0 26-27 0.0 0.0 0.0 0.0377469546524721 0.0 28-29 0.0 0.0 0.0 0.047343638038693826 0.0 30-31 0.0 0.0 0.0 0.06065103900092128 0.0 32-33 0.0 0.0 0.0 0.08281297983416931 0.0 34-35 0.0 0.0 0.0 0.1135991401371686 0.0 36-37 0.0 0.0 0.0 0.15288156413143617 0.0 38-39 0.0 0.0 0.0 0.21481216091718702 0.0 40-41 0.0 0.0 0.0 0.29066434640188354 0.0 42-43 0.0 0.0 0.0 0.3685126420309141 0.0 44-45 0.0 0.0 0.0 0.45936124475381307 0.0 46-47 0.0 0.0 0.0 0.5694288054048521 0.0 48-49 0.0 0.0 0.0 0.6805456034394513 0.0 50-51 0.0 0.0 0.0 0.8069659125806121 0.0 52-53 0.0 0.0 0.0 0.9259136042583682 0.0 54-55 0.0 0.0 0.0 1.0531272392261235 0.0 56-57 0.0 0.0 0.0 1.2260978605793837 0.0 58-59 0.0 0.0 0.0 1.4116081482239737 0.0 60-61 0.0 0.0 0.0 1.6010338826901425 0.0 62-63 0.0 0.0 0.0 1.7857252533524415 0.0 64-65 0.0 0.0 0.0 1.980397174736411 0.0 66-67 0.0 0.0 0.0 2.2052922509980553 0.0 68-69 0.0 0.0 0.0 2.477377418364213 0.0 70-71 0.0 0.0 0.0 2.7774593100624427 0.0 72-73 0.0 0.0 0.0 3.0696591258061217 0.0 74-75 0.0 0.0 0.0 3.3752942982905108 0.0 76-77 0.0 0.0 0.0 3.6871225304534754 0.0 78-79 0.0 0.0 0.0 4.109351008291535 0.0 80-81 0.0 0.0 0.0 4.575391544682158 0.0 82-83 0.0 0.0 0.0 5.016557477735694 0.0 84-85 0.0 0.0 0.0 5.43904186713072 0.0 86-87 0.0 0.0 0.0 5.883841744293172 0.0 88-89 0.0 0.0 0.0 6.404237895383355 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AGTACGT 35 2.7881528E-4 54.286797 2 GACATAT 805 0.0 49.008743 1 GTCTCGC 95 1.9308573E-8 39.996704 9 TACCTGG 325 0.0 38.00076 2 ACATATA 1160 0.0 35.62571 2 TAACTTT 1600 0.0 34.731514 6 GTCGGGA 55 0.0025798134 34.546143 2 GGTCTCG 110 7.022027E-8 34.542606 8 ACTTAAT 3410 0.0 33.03584 1 CATATAG 1165 0.0 33.025562 3 CTTAATA 3435 0.0 32.911865 2 GTGTAGC 555 0.0 32.54483 1 AACTTTA 1725 0.0 32.214737 7 ATCTATA 1710 0.0 31.688385 1 GCGTCAT 30 0.0039536976 31.664057 10-11 ACTTTAT 1780 0.0 31.219337 8 ATAACTT 2015 0.0 29.935497 5 CTATAAC 1955 0.0 29.88474 3 TAATACT 4255 0.0 29.35866 4 ACTTCAG 3820 0.0 29.34313 8 >>END_MODULE