##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031596_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 114962 Sequences flagged as poor quality 0 Sequence length 101 %GC 35 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.910561750839406 33.0 31.0 34.0 30.0 34.0 2 32.002896609314384 33.0 31.0 34.0 30.0 34.0 3 32.03872583984273 34.0 31.0 34.0 30.0 34.0 4 35.17042153059272 37.0 35.0 37.0 33.0 37.0 5 35.08235764861433 37.0 35.0 37.0 32.0 37.0 6 35.4087611558602 37.0 35.0 37.0 33.0 37.0 7 35.555531392982026 37.0 35.0 37.0 33.0 37.0 8 35.592317461422034 37.0 36.0 37.0 33.0 37.0 9 37.37902089386058 39.0 38.0 39.0 35.0 39.0 10-11 37.399697291278855 39.0 38.0 39.0 35.0 39.0 12-13 37.42844157199771 39.0 38.0 39.0 35.0 39.0 14-15 38.58509768445225 41.0 38.0 41.0 34.5 41.0 16-17 38.57281101581392 40.0 38.0 41.0 34.5 41.0 18-19 38.689906229884656 41.0 38.5 41.0 35.0 41.0 20-21 38.787895130564884 41.0 39.0 41.0 35.0 41.0 22-23 38.62772046415337 41.0 38.5 41.0 35.0 41.0 24-25 38.51290861328091 41.0 38.0 41.0 35.0 41.0 26-27 38.20852977505611 40.0 37.5 41.0 34.5 41.0 28-29 37.93796645848193 40.0 36.5 41.0 34.0 41.0 30-31 37.527504740696926 40.0 35.5 41.0 34.0 41.0 32-33 36.9776969781319 39.0 35.0 41.0 33.0 41.0 34-35 36.63416172300413 39.0 35.0 41.0 32.5 41.0 36-37 36.277187244480785 39.0 35.0 41.0 30.5 41.0 38-39 35.73357718202537 38.0 35.0 41.0 29.5 41.0 40-41 35.34745394130235 38.0 35.0 41.0 25.5 41.0 42-43 34.83762460639168 38.0 35.0 41.0 20.5 41.0 44-45 34.15046711087142 38.0 35.0 41.0 13.5 41.0 46-47 33.246559732781265 38.0 33.5 40.5 9.0 41.0 48-49 32.662632000139176 38.0 33.0 41.0 7.5 41.0 50-51 32.12230563142604 37.5 32.0 40.5 7.0 41.0 52-53 32.01779718515684 38.0 31.5 40.5 7.0 41.0 54-55 31.968489587863814 38.0 31.0 41.0 7.0 41.0 56-57 31.65219376837564 38.0 29.0 41.0 7.0 41.0 58-59 31.299016196656286 38.0 26.5 41.0 7.0 41.0 60-61 30.854799846905934 37.0 25.0 41.0 7.0 41.0 62-63 30.422661401158642 36.0 22.0 40.5 7.0 41.0 64-65 30.111393329969903 36.0 20.0 40.0 7.0 41.0 66-67 29.77883126598354 35.5 19.5 40.0 7.0 41.0 68-69 29.389145978671213 35.0 18.5 39.0 7.0 41.0 70-71 28.953380247386093 35.0 16.0 39.0 7.0 41.0 72-73 28.47800142655834 35.0 13.5 38.5 7.0 41.0 74-75 28.000561054957288 35.0 10.5 37.0 7.0 40.0 76-77 27.521724569857867 35.0 10.0 37.0 7.0 39.0 78-79 27.124867347471337 35.0 10.0 36.0 7.0 39.0 80-81 26.804361441171864 35.0 10.0 36.0 7.0 38.5 82-83 26.44423374680329 34.5 10.0 35.0 7.0 37.0 84-85 26.154551069048903 34.0 10.0 35.0 7.0 37.0 86-87 25.89915798263774 34.0 10.0 35.0 7.0 36.5 88-89 25.57859988517945 34.0 10.0 35.0 7.0 36.0 90-91 25.32081905325238 34.0 9.0 35.0 7.0 36.0 92-93 25.109640576886274 34.0 8.0 35.0 7.0 36.0 94-95 24.904177032410708 34.0 8.0 35.0 7.0 35.0 96-97 24.74978253683826 34.0 8.0 35.0 7.0 35.0 98-99 24.641185783128336 34.0 8.0 35.0 7.0 35.0 100-101 24.244585167272664 32.5 8.0 35.0 6.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.22677561417784986 1101 2 0.38754351671170895 1101 3 0.5841797326172156 1101 4 0.5693694407750485 1101 5 -0.1804515750006388 1101 6 0.09863004324957814 1101 7 -0.04265184111036291 1101 8 0.33165035071230164 1101 9 0.3534088616846631 1101 10-11 0.3982818518718574 1101 12-13 0.3109865405303225 1101 14-15 0.4703716392927859 1101 16-17 0.9250913256045266 1101 18-19 0.6721650797780967 1101 20-21 0.7618472782548125 1101 22-23 0.5224726558188166 1101 24-25 0.5864443508218002 1101 26-27 0.8887738075480485 1101 28-29 0.7346343819192285 1101 30-31 0.4972272462638756 1101 32-33 0.7162832905748004 1101 34-35 0.10947575302350288 1101 36-37 0.6680240591864717 1101 38-39 0.2891070584618234 1101 40-41 0.48569399742137875 1101 42-43 0.8950450991069445 1101 44-45 0.9773732616500936 1101 46-47 0.8032975006145691 1101 48-49 0.5068166722302863 1101 50-51 0.5731962327566293 1101 52-53 1.1114460498133596 1101 54-55 0.6507721921805043 1101 56-57 0.21943337024504572 1101 58-59 0.5547281290003667 1101 60-61 1.1054722593029105 1101 62-63 1.031473236823384 1101 64-65 1.3105034629415613 1101 66-67 0.9448102548527473 1101 68-69 1.4108848482962628 1101 70-71 1.1580230718081452 1101 72-73 1.2840289600577428 1101 74-75 0.6355893799543004 1101 76-77 0.7268266384664379 1101 78-79 0.360134891282442 1101 80-81 0.26235872122729376 1101 82-83 0.5361075777374822 1101 84-85 0.921526305097295 1101 86-87 1.1362523501158357 1101 88-89 1.463858906306175 1101 90-91 1.359317215987602 1101 92-93 1.318398307282287 1101 94-95 1.4437937153063665 1101 96-97 1.4896829735526254 1101 98-99 1.60350986122182 1101 100-101 1.8997161892993368 1102 1 0.12498915800834354 1102 2 -0.40029750253770047 1102 3 -0.46802910272414877 1102 4 -0.2971310439223629 1102 5 0.1754548091566832 1102 6 0.47129310930690593 1102 7 -0.187507124312134 1102 8 0.14926563945347482 1102 9 -0.4999355504466223 1102 10-11 -0.21299082504269506 1102 12-13 0.018160062203222083 1102 14-15 0.0794285566172519 1102 16-17 0.294776964712689 1102 18-19 0.5776490847635642 1102 20-21 0.6446889899290937 1102 22-23 0.4943602060196284 1102 24-25 0.7732605812607929 1102 26-27 0.8836498634959895 1102 28-29 0.9132799988697897 1102 30-31 1.078310198763539 1102 32-33 1.0406151005734117 1102 34-35 1.2667946839194997 1102 36-37 0.8988092689912079 1102 38-39 1.1042988601513457 1102 40-41 1.1214092723140539 1102 42-43 1.2500513309307948 1102 44-45 0.49468388344762815 1102 46-47 0.8657542240600691 1102 48-49 1.4176323487293843 1102 50-51 2.128590222508741 1102 52-53 2.6818656592857337 1102 54-55 3.2693845727372164 1102 56-57 2.9050032359146236 1102 58-59 3.508889602480494 1102 60-61 2.735786620194748 1102 62-63 2.8597518685918395 1102 64-65 3.386047016465998 1102 66-67 3.3167808960381784 1102 68-69 3.6120342735836175 1102 70-71 3.610176513159761 1102 72-73 2.905203312640488 1102 74-75 2.91796024271569 1102 76-77 3.214086020823455 1102 78-79 3.226219921056714 1102 80-81 3.8012729990281535 1102 82-83 3.4865299569093438 1102 84-85 2.8418974448286356 1102 86-87 2.6863839108414957 1102 88-89 2.8590257807003034 1102 90-91 2.40785619950789 1102 92-93 3.529726378186595 1102 94-95 3.066075947684155 1102 96-97 3.7729539886474903 1102 98-99 3.767129858452119 1102 100-101 3.5821701430453565 1103 1 0.14502435497598398 1103 2 -0.003519378806821294 1103 3 -0.45327345090338156 1103 4 0.18127697015642497 1103 5 0.4513448863192622 1103 6 0.009129849526210876 1103 7 -0.12117437759493299 1103 8 0.017818502591417484 1103 9 -0.32733503602940317 1103 10-11 0.0709539427926913 1103 12-13 0.05707978604239372 1103 14-15 0.05154172840553173 1103 16-17 -0.002908157799843991 1103 18-19 -0.19668420344545723 1103 20-21 -0.4138598120202772 1103 22-23 -0.34644120185908633 1103 24-25 0.44315363662833107 1103 26-27 0.4698147477521104 1103 28-29 0.1400973807568775 1103 30-31 0.2094189032443765 1103 32-33 0.6998813791709537 1103 34-35 -0.13242015295609377 1103 36-37 -0.7963308420146049 1103 38-39 -0.363482377157446 1103 40-41 0.24825372879164576 1103 42-43 0.6536657887621189 1103 44-45 0.8794138144074211 1103 46-47 0.9033879048506535 1103 48-49 -0.5639569297327824 1103 50-51 -0.8402739984199172 1103 52-53 -0.5815024197720717 1103 54-55 0.09827316079731929 1103 56-57 0.26773506209574904 1103 58-59 -0.17108873784711065 1103 60-61 -0.348550729202838 1103 62-63 -0.05137578810235155 1103 64-65 0.20505337899477283 1103 66-67 -0.5825693137898966 1103 68-69 0.005292700549908602 1103 70-71 -0.14831813960861595 1103 72-73 0.13202069452758636 1103 74-75 0.02077600012735914 1103 76-77 -0.05936734319441683 1103 78-79 -0.2066350945111779 1103 80-81 -0.5254496217922089 1103 82-83 -0.5081904979437759 1103 84-85 0.02045436915515708 1103 86-87 -0.3534157371888291 1103 88-89 -0.3861346362483893 1103 90-91 -0.6155633212718605 1103 92-93 -0.6059850066787078 1103 94-95 -0.7368210334990053 1103 96-97 -0.7800445222500336 1103 98-99 -0.44750395771140816 1103 100-101 -0.2668213284586365 1104 1 0.10884457969509143 1104 2 0.45524466613699843 1104 3 0.2075130659505504 1104 4 0.2092544982463096 1104 5 0.3944909537349943 1104 6 0.1258714225599178 1104 7 0.3330952853264151 1104 8 0.4961331093329946 1104 9 0.37030541340880063 1104 10-11 0.14718989784886816 1104 12-13 0.4171359658176783 1104 14-15 0.6674406048100252 1104 16-17 0.27911431411027365 1104 18-19 0.41337197632982736 1104 20-21 0.6578817610134351 1104 22-23 0.3258059891521512 1104 24-25 0.2400075692126009 1104 26-27 0.4000956466285146 1104 28-29 0.34273783019508386 1104 30-31 0.33475598189605904 1104 32-33 0.516398233103537 1104 34-35 0.6701079369315508 1104 36-37 0.7842309557382023 1104 38-39 0.5438771734043542 1104 40-41 0.40460204339839123 1104 42-43 0.6786657887621175 1104 44-45 0.9031778593512385 1104 46-47 1.1523204891203065 1104 48-49 0.8273913848739625 1104 50-51 0.24377094540030697 1104 52-53 0.4452391532616389 1104 54-55 0.9745652956287785 1104 56-57 1.2538586576013735 1104 58-59 1.4558775542877243 1104 60-61 1.7614492707971614 1104 62-63 2.064691627627983 1104 64-65 1.9979747273093729 1104 66-67 1.6364194502550475 1104 68-69 1.712034273583619 1104 70-71 1.950839163762165 1104 72-73 1.6296611439657873 1104 74-75 1.476393977655448 1104 76-77 1.5711944545583911 1104 78-79 1.9338705234663536 1104 80-81 1.76488745685948 1104 82-83 1.564843209921392 1104 84-85 1.6388251556720093 1104 86-87 1.6410224650583665 1104 88-89 1.5291462626280143 1104 90-91 1.3821333079416256 1104 92-93 1.0133408360179175 1104 94-95 1.3764373934672847 1104 96-97 1.5968094103342345 1104 98-99 1.4183949186930853 1104 100-101 1.373796649069451 1105 1 0.09915385804560728 1105 2 0.19143023314730812 1105 3 0.28864708656910665 1105 4 0.23224418896796095 1105 5 0.14067652074530201 1105 6 -0.17794301042977168 1105 7 0.030105594604769692 1105 8 -0.18025864324432916 1105 9 -0.12010695772521984 1105 10-11 0.24198371228185778 1105 12-13 0.17192978025066452 1105 14-15 0.38914163573786453 1105 16-17 0.33787720070821337 1105 18-19 0.21198022375250503 1105 20-21 0.34236629709590716 1105 22-23 0.417661659255252 1105 24-25 0.11464674447033474 1105 26-27 0.19870389405119226 1105 28-29 0.5535110260713765 1105 30-31 0.3211992808651374 1105 32-33 0.13588276918600428 1105 34-35 0.34510793693154795 1105 36-37 0.41917940934644804 1105 38-39 0.5272276888682725 1105 40-41 0.7778494660788056 1105 42-43 0.1021193970095382 1105 44-45 0.8011675500728899 1105 46-47 0.8604132726254647 1105 48-49 0.006875920956431969 1105 50-51 0.10933795570958438 1105 52-53 0.8499814213028714 1105 54-55 0.5336374605772392 1105 56-57 0.4049411318281706 1105 58-59 0.9517538429475181 1105 60-61 1.2029956625497427 1105 62-63 1.5276813183496358 1105 64-65 1.0586963767939146 1105 66-67 1.0975534708736028 1105 68-69 1.1326528302846484 1105 70-71 0.44851957613330384 1105 72-73 0.6072900099451708 1105 74-75 0.629486761160603 1105 76-77 0.68191610404293 1105 78-79 0.30124165748696896 1105 80-81 0.24096993108627984 1105 82-83 0.14216279755025596 1105 84-85 0.24650556804314405 1105 86-87 0.8589090629965064 1105 88-89 0.5489400770610011 1105 90-91 0.6343498027869856 1105 92-93 0.36117588756430763 1105 94-95 0.3832415171786252 1105 96-97 0.26773724538578136 1105 98-99 0.3076217228167941 1105 100-101 0.17694097896636052 1106 1 0.49478517375449727 1106 2 0.35158129980036534 1106 3 0.1551368283267891 1106 4 0.3042049932958193 1106 5 0.16221372601221873 1106 6 0.08121795721338287 1106 7 0.2551744932472104 1106 8 0.06910340636269297 1106 9 0.2632757104384993 1106 10-11 0.32476415527461455 1106 12-13 0.675502302451342 1106 14-15 0.5057079315426947 1106 16-17 0.6711935220310679 1106 18-19 0.7138175208842767 1106 20-21 0.20773324616194344 1106 22-23 0.5835287614293847 1106 24-25 0.4897105395096233 1106 26-27 0.02232336940079449 1106 28-29 0.3247675331653781 1106 30-31 0.20213221951982518 1106 32-33 -0.6027106777875488 1106 34-35 -0.21429800366251328 1106 36-37 -0.15170963832120066 1106 38-39 0.1944217278597975 1106 40-41 0.5557406572597827 1106 42-43 0.49524994717796034 1106 44-45 0.1602570672720347 1106 46-47 0.2721719742688222 1106 48-49 -0.09310366463098774 1106 50-51 0.39901847015278236 1106 52-53 -0.3509984705007412 1106 54-55 -0.5641475756583461 1106 56-57 -0.24005223348774152 1106 58-59 0.0034023067629718184 1106 60-61 -0.6010259767275876 1106 62-63 -0.06867470900568051 1106 64-65 0.9273806679034386 1106 66-67 0.6323600443144564 1106 68-69 0.07243031318757787 1106 70-71 0.14465104495028314 1106 72-73 0.6681759954509374 1106 74-75 -0.19142780452277108 1106 76-77 -0.10286495138220175 1106 78-79 -0.4923175953455292 1106 80-81 -0.4287759094771566 1106 82-83 -0.4896122356231629 1106 84-85 -0.17122434927848573 1106 86-87 0.19998286109796837 1106 88-89 0.29508685668742274 1106 90-91 -0.3223716425534242 1106 92-93 -0.18052055012069346 1106 94-95 -0.006928943166379042 1106 96-97 0.10809653904710714 1106 98-99 0.7739889780990232 1106 100-101 1.402162985703118 1107 1 -0.707615597296062 1107 2 -0.8997110860753885 1107 3 -0.4276196774122809 1107 4 -0.11224992653244925 1107 5 -0.8708187807782792 1107 6 -1.0832436216878705 1107 7 -0.8643383429921698 1107 8 -0.16484034199444153 1107 9 0.38331426296632287 1107 10-11 0.28572972085772363 1107 12-13 0.04753419590617369 1107 14-15 0.29704237472152784 1107 16-17 0.31716741145540794 1107 18-19 0.3911153391616864 1107 20-21 0.17518264596917987 1107 22-23 0.006248467028264315 1107 24-25 -0.08424021839802265 1107 26-27 -0.0026919639909550597 1107 28-29 0.005613936389771368 1107 30-31 -0.29475729244021664 1107 32-33 -0.46050442176372286 1107 34-35 -0.09294516041358492 1107 36-37 -0.878512407093659 1107 38-39 -1.2334237115513957 1107 40-41 -1.7153537088139998 1107 42-43 -1.8330598749546922 1107 44-45 -2.007928335339024 1107 46-47 -2.149759156897389 1107 48-49 -2.1470333938870994 1107 50-51 -1.7930432138917283 1107 52-53 -1.9703360679772999 1107 54-55 -2.2894170052561726 1107 56-57 -2.3346811654074813 1107 58-59 -2.06270651650874 1107 60-61 -1.8397011716807121 1107 62-63 -2.418582708655201 1107 64-65 -1.8753881045490282 1107 66-67 -1.6408371869130924 1107 68-69 -1.6269037795137251 1107 70-71 -1.9653112787157099 1107 72-73 -1.5201618648837716 1107 74-75 -1.2700661993357016 1107 76-77 -1.460132979069929 1107 78-79 -1.832368414586746 1107 80-81 -1.503077144910435 1107 82-83 -1.324537321052059 1107 84-85 -1.6540509505226844 1107 86-87 -1.6075173579504813 1107 88-89 -1.4382873656905737 1107 90-91 -1.52950386019997 1107 92-93 -1.4471901374334095 1107 94-95 -1.2506422525504135 1107 96-97 -1.6702702356834642 1107 98-99 -1.6754546388290414 1107 100-101 -1.5001414040278895 1108 1 -0.45579827744776935 1108 2 -0.24689819100585808 1108 3 -0.6539155054780217 1108 4 -0.5489597874679717 1108 5 -0.5030090462650065 1108 6 -0.3573428631543649 1108 7 -0.08154757181643646 1108 8 -0.696366890667008 1108 9 -0.37719458659120164 1108 10-11 -0.864238673579699 1108 12-13 -0.3226854627537534 1108 14-15 -0.551487966618545 1108 16-17 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-1.3301715464764818 2316 56-57 -1.110673506141321 2316 58-59 -1.7558183521450275 2316 60-61 -0.9099542379747696 2316 62-63 -0.76788147178722 2316 64-65 -1.7636042200590438 2316 66-67 -1.969486982493489 2316 68-69 -1.7505388258315868 2316 70-71 -1.2168508947173642 2316 72-73 -1.4861868092505865 2316 74-75 -1.1665884784849005 2316 76-77 -0.22225583783926695 2316 78-79 -0.5087902952470991 2316 80-81 -0.6313698530820453 2316 82-83 -1.4000690707803614 2316 84-85 -1.5796836162578174 2316 86-87 -0.9332173010235074 2316 88-89 -1.2804443806468377 2316 90-91 -0.9541532417659759 2316 92-93 -1.4350802166136596 2316 94-95 -1.5102292731993785 2316 96-97 -1.1871671978528973 2316 98-99 -0.8946752567455114 2316 100-101 -1.2553553977141725 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 1.0 7 14.0 8 103.0 9 136.0 10 116.0 11 144.0 12 179.0 13 222.0 14 324.0 15 564.0 16 1298.0 17 2199.0 18 2520.0 19 3446.0 20 4178.0 21 4514.0 22 3147.0 23 2574.0 24 2448.0 25 1942.0 26 1773.0 27 1828.0 28 1868.0 29 1805.0 30 1766.0 31 2017.0 32 2369.0 33 2937.0 34 4154.0 35 8376.0 36 9018.0 37 14311.0 38 24442.0 39 8223.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 50.57399965053294 22.11602306482614 13.354883802201641 13.95509348243928 2 15.46088519982893 25.29042619116197 41.016644410114075 18.23204419889503 3 20.27971418612757 31.574590449634023 35.808644126873475 12.337051237364935 4 9.375599212090576 21.161120504819845 41.84548608084788 27.617794202241708 5 7.350838651183876 45.23747253896851 35.04201973707152 12.369669072776093 6 19.174835533481463 41.6572996993857 19.838801028188037 19.3290637389448 7 19.413102384707205 42.86373972972737 23.113450027445477 14.609707858119942 8 24.134355667857456 33.6995730591618 22.85963230809445 19.306438964886294 9 27.07423961246591 17.0765920001394 24.55718480183312 31.291983585561567 10-11 21.85542126680667 28.180305155932768 29.69519261770113 20.26908095955943 12-13 26.46138832980457 30.583509771632205 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15.191541552860945 35.15944399018806 35.21120022268228 14.437814234268714 40-41 13.651902367738906 35.85880551834519 35.85141177084602 14.637880343069885 42-43 13.728014474348047 37.05963709747569 34.68580922391747 14.526539204258798 44-45 15.536750230832217 34.89878225118029 34.15010191460079 15.4143656033867 46-47 14.947808843077862 35.26989038767461 33.809409662446335 15.972891106801193 48-49 15.038762389382457 33.984233871494304 33.84276529710577 17.134238442017473 50-51 14.236718634389229 34.213824981067035 33.78511303197277 17.764343352570965 52-53 15.165509586715705 35.18509652005484 32.65794685412087 16.991447039108575 54-55 15.230620559966567 33.48307563727539 31.609555648419 19.676748154339045 56-57 16.178423832811486 34.392704881267065 31.381813589510216 18.04705769641123 58-59 16.630530376252796 32.21180219952457 31.227414818491333 19.9302526057313 60-61 16.521549752814863 32.40891173203824 32.26299627588911 18.80654223925779 62-63 15.146336252937406 31.5125409275011 32.53142316529261 20.809699654268883 64-65 14.368897366885294 31.655299763951678 33.60422273903159 20.371580130131438 66-67 16.083045223767513 30.03727281435874 32.490050422802604 21.38963153907114 68-69 16.073590246026562 30.00174178097104 33.26409753973438 20.660570433268017 70-71 15.844670338042272 30.058988702901544 33.386299819333495 20.710041139722687 72-73 16.88459228177695 28.464538482297534 33.53805573208207 21.11281350384344 74-75 15.751001916710228 30.23828193064994 32.1689318696637 21.84178428297613 76-77 17.00634304771454 29.140091985090567 32.28807463434837 21.565490332846522 78-79 16.61584569009078 29.624854071195834 32.80871565227997 20.95058458643341 80-81 17.58746936341197 28.57484904814352 32.7200936837477 21.117587904696812 82-83 17.06205293198253 29.850102964417413 32.166258146814634 20.92158595678542 84-85 16.748667665192098 30.443414956982128 30.94543522937058 21.86248214845519 86-87 17.443859717851282 30.086577260084358 30.06873076604987 22.400832256014485 88-89 18.97310641188072 29.513285702468185 29.471488965225983 22.042118920425107 90-91 18.728061388915503 28.64888641134406 30.88651586548093 21.736536334259508 92-93 19.860393373478228 29.640264572857756 29.757474880393197 20.74186717327082 94-95 19.544300268750465 30.14186714057348 29.10824502027607 21.205587570399988 96-97 21.112939598951666 28.6741721738979 27.70115543017353 22.5117327969769 98-99 21.03719051432949 29.98789915381133 26.439913640004182 22.534996691855 100-101 22.263134300425225 29.053233577500098 26.474466946087453 22.209165175987224 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 863.0 1 540.5 2 331.5 3 618.5 4 516.0 5 325.0 6 551.0 7 1042.0 8 1283.5 9 972.0 10 584.5 11 350.0 12 304.5 13 288.0 14 250.5 15 245.5 16 237.0 17 225.5 18 269.5 19 314.5 20 323.5 21 364.0 22 448.0 23 526.0 24 653.0 25 827.5 26 1264.5 27 1949.0 28 2398.0 29 2916.5 30 3977.0 31 4891.0 32 5430.0 33 6429.0 34 7936.5 35 8037.5 36 6520.5 37 5420.5 38 4334.0 39 3150.5 40 2442.0 41 1834.5 42 1496.0 43 1516.0 44 1440.0 45 1283.0 46 1446.5 47 2212.5 48 4921.0 49 5277.0 50 3972.0 51 3142.5 52 1406.5 53 828.5 54 635.0 55 543.0 56 544.0 57 482.0 58 353.5 59 290.5 60 293.5 61 272.0 62 195.5 63 110.5 64 73.0 65 68.5 66 61.5 67 56.5 68 45.5 69 37.5 70 33.5 71 36.5 72 29.0 73 16.0 74 11.5 75 10.5 76 15.0 77 13.0 78 8.0 79 4.0 80 5.0 81 7.0 82 5.0 83 3.0 84 2.0 85 2.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.5 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.43666602877472555 2 0.3383726796680642 3 0.17571023468624414 4 0.19832640350724587 5 0.2209425723282476 6 0.17136097145143614 7 0.1644021502757433 8 0.16701170821662809 9 0.16092273968789686 10-11 0.17484038203928254 12-13 0.16614185556966649 14-15 0.17875471895060976 16-17 0.0743724013152172 18-19 0.03522903220194499 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13917642351385676 46-47 0.20832970894730432 48-49 0.08307092778483324 50-51 0.07219776969781319 52-53 0.07785181190306362 54-55 0.08524555940223727 56-57 0.12656356013291348 58-59 0.10351246498843097 60-61 0.147440023659992 62-63 0.2413841095318453 64-65 0.13482716027904873 66-67 0.11569040204589343 68-69 0.11916981263373984 70-71 0.09524886484229572 72-73 0.07915659087350603 74-75 0.15831318174701206 76-77 0.23442528835615245 78-79 0.15657347645308886 80-81 0.09350915954837251 82-83 0.10133783337102693 84-85 0.1096014335171622 86-87 0.08133122249091004 88-89 0.10481724395887337 90-91 0.1330874549851255 92-93 0.1835389085088986 94-95 0.1491797289539152 96-97 0.09829334910666132 98-99 0.08176614881439084 100-101 0.07089299072737078 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 114962.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 31.82529879438423 #Duplication Level Percentage of deduplicated Percentage of total 1 79.58291196326564 25.327499521581043 2 7.909913357203378 5.034707120613769 3 3.2060567961297726 3.061011464657887 4 2.0417088036734357 2.599119709121275 5 1.3556727799491624 2.1572345644647797 6 0.9456910924645366 1.8058140950922914 7 0.563041517478886 1.254327516918634 8 0.5083772924809359 1.294340738678868 9 0.38264957498565066 1.0960143351716218 >10 2.820674009894225 18.063360066804684 >50 0.3498510399868806 7.872166455002523 >100 0.297920026238828 18.9192950714149 >500 0.019132478749282533 4.441467615385954 >1k 0.01639926749938503 7.07364172509177 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1932 1.6805553139298202 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1638 1.4248186357231085 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1289 1.1212400619335083 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1107 0.9629268801864965 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1101 0.9577077643047268 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 1065 0.926393069014109 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 869 0.7559019502096345 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 848 0.7376350446234409 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 803 0.6984916755101686 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 754 0.65586889580905 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 655 0.5697534837598511 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 593 0.5158226196482316 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 584 0.5079939458255771 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 500 0.43492632348080235 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 493 0.4288373549520711 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 483 0.42013882848245504 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 468 0.407091038778031 No Hit GTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAAAACATTTATCCT 448 0.3896939858387989 No Hit ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA 442 0.3844748699570293 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 428 0.3722969328995668 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 365 0.3174962161409857 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 361 0.31401680555313927 No Hit ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA 346 0.3009690158487152 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 343 0.2983594579078304 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 327 0.2844418155564447 No Hit GTACTAGTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAAAACATT 326 0.2835719629094831 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 323 0.2809624049685983 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 320 0.2783528470277135 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 308 0.26791461526417426 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 308 0.26791461526417426 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 299 0.2600859414415198 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 298 0.2592160887945582 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 283 0.24616829909013413 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 280 0.24355874114924933 No Hit AATCTAAACTTACTTTTTGATTTTGTTGTTTTTTTAGCAAGTTTAAAATT 260 0.22616168821001723 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 253 0.22007271968128597 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 247 0.21485360379951637 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 242 0.21050434056470832 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 241 0.20963448791774675 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 234 0.2035455193890155 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 226 0.19658669821332264 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 225 0.19571684556636107 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 222 0.19310728762547624 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 221 0.19223743497851464 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 221 0.19223743497851464 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 220 0.19136758233155304 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 219 0.19049772968459144 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 217 0.18875802439066822 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 213 0.18527861380282182 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 209 0.1817992032149754 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 208 0.18092935056801376 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 206 0.17918964527409056 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 201 0.17484038203928254 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 200 0.17397052939232094 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 198 0.1722308240983977 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 193 0.1678815608635897 No Hit CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA 193 0.1678815608635897 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 191 0.16614185556966649 No Hit ACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTAT 189 0.1644021502757433 No Hit GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT 189 0.1644021502757433 No Hit GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC 186 0.1617925923348585 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 185 0.16092273968789686 No Hit TATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 185 0.16092273968789686 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 184 0.16005288704093526 No Hit GTACATGGGCTTGATACCACTGCTTCCAAAAAAAAAAAAAAAAAAAAAAA 181 0.15744332910005046 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 178 0.15483377115916563 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 177 0.15396391851220403 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 173 0.1504845079243576 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 172 0.149614655277396 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 169 0.14700509733651118 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 167 0.14526539204258798 No Hit GGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTA 166 0.14439553939562638 No Hit ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 163 0.14178598145474156 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 162 0.14091612880777996 No Hit CCATAATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACT 160 0.13917642351385676 No Hit GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT 158 0.13743671821993356 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 151 0.13134774969120233 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAA 150 0.1304778970442407 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 146 0.12699848645639428 No Hit GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT 141 0.12264922322158626 No Hit TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT 140 0.12177937057462467 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 137 0.11916981263373984 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 136 0.11829995998677822 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 135 0.11743010733981664 No Hit ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC 134 0.11656025469285504 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 134 0.11656025469285504 No Hit GTGGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 133 0.11569040204589343 No Hit GGTTAAAAGCTGTTTTGTTAATTATTCACTATTAAAGGTTTTTTCCGTTC 133 0.11569040204589343 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 132 0.11482054939893183 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 131 0.11395069675197021 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 130 0.11308084410500861 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 130 0.11308084410500861 No Hit GGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGG 129 0.112210991458047 No Hit GTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTAT 128 0.11134113881108539 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 128 0.11134113881108539 No Hit GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT 127 0.1104712861641238 No Hit CTATAGCTCCTAGATGTACGAATTTCTTTCTCCAAAACATTTATCCTACT 124 0.10786172822323899 No Hit ATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGAATTAATTGAAATTTTA 123 0.10699187557627737 No Hit ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA 123 0.10699187557627737 No Hit GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC 123 0.10699187557627737 No Hit GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 121 0.10525217028235416 No Hit TATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 119 0.10351246498843097 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAA 118 0.10264261234146936 No Hit GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC 117 0.10177275969450775 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 8.698526469616047E-4 0.0 10-11 0.0 0.0 0.0 0.0017397052939232094 0.0 12-13 0.0 0.0 0.0 0.00739374749917364 0.0 14-15 0.0 0.0 0.0 0.012177937057462465 0.0 16-17 0.0 0.0 0.0 0.03783859014282981 0.0 18-19 0.0 0.0 0.0 0.06132461161079314 0.0 20-21 0.0 0.0 0.0 0.10568709660583497 0.0 22-23 0.0 0.0 0.0 0.23355543570919088 0.0 24-25 0.0 0.0 0.0 0.3666428906943164 0.0 26-27 0.0 0.0 0.0 0.4410152920095336 0.0 28-29 0.0 0.0 0.0 0.5027748299438075 0.0 30-31 0.0 0.0 0.0 0.6001983264035073 0.0 32-33 0.0 0.0 0.0 0.7593813607974809 0.0 34-35 0.0 0.0 0.0 0.9942415754771141 0.0 36-37 0.0 0.0 0.0 1.2595466328004037 0.0 38-39 0.0 0.0 0.0 1.5465980062977331 0.0 40-41 0.0 0.0 0.0 1.9680416137506307 0.0 42-43 0.0 0.0 0.0 2.224648144604304 0.0 44-45 0.0 0.0 0.0 2.4642925488422263 0.0 46-47 0.0 0.0 0.0 2.7626520067500566 0.0 48-49 0.0 0.0 0.0 3.0775386649501573 0.0 50-51 0.0 0.0 0.0 3.3754631965345068 0.0 52-53 0.0 0.0 0.0 3.6229362745950837 0.0 54-55 0.0 0.0 0.0 3.8399645100120043 0.0 56-57 0.0 0.0 0.0 4.086132809102137 0.0 58-59 0.0 0.0 0.0 4.364485656129851 0.0 60-61 0.0 0.0 0.0 4.64544806109845 0.0 62-63 0.0 0.0 0.0 4.875089159896314 0.0 64-65 0.0 0.0 0.0 5.0847236478140605 0.0 66-67 0.0 0.0 0.0 5.320453715140655 0.0 68-69 0.0 0.0 0.0 5.624467215253736 0.0 70-71 0.0 0.0 0.0 6.0350376646196136 0.0 72-73 0.0 0.0 0.0 6.367756302082427 0.0 74-75 0.0 0.0 0.0 6.644369443816217 0.0 76-77 0.0 0.0 0.0 6.946643238635375 0.0 78-79 0.0 0.0 0.0 7.295889076390459 0.0 80-81 0.0 0.0 0.0 7.692106957081471 0.0 82-83 0.0 0.0 0.0 8.052225952923575 0.0 84-85 0.0 0.0 0.0 8.44496442302674 0.0 86-87 0.0 0.0 0.0 8.856404725039578 0.0 88-89 0.0 0.0 0.0 9.295680311755188 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAACGC 30 1.3002688E-4 63.293827 4 AAACGCA 30 1.3002688E-4 63.293827 5 CTACATA 25 0.004978988 57.08882 2 AAGTACT 35 2.7859255E-4 54.251854 4 ATAAACG 35 2.7859255E-4 54.251854 3 AAAGTAC 40 5.3843413E-4 47.47037 3 AGTACTC 40 5.3843413E-4 47.47037 5 ATGGGCA 55 4.9535178E-5 43.154884 5 CATGGCC 45 9.618331E-4 42.19588 4 ATAGATT 140 0.0 40.68889 4 ATGGCCG 50 0.0016147023 37.976295 5 ACATGGC 50 0.0016147023 37.976295 3 TGGGCAG 50 0.0016147023 37.976295 6 GTTATAG 350 0.0 36.780262 1 CTATGCC 65 1.325604E-4 36.51567 9 TTAACAG 65 1.325604E-4 36.51567 4 ACTTTAT 350 0.0 35.263706 8 CATGGGG 270 0.0 35.163235 4 GGCCGGA 55 0.0025778764 34.523907 7 TATAGGT 180 0.0 34.284153 3 >>END_MODULE