FastQCFastQC Report
Fri 10 Feb 2017
SRR2031594_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031594_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095086
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT57860.5283603296910014No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT33130.30253331701802416No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT23570.21523423731104224No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC18520.16911913767503192No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16580.1514036340524854No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT16380.1495772934728414No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15480.14135876086444352No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT14190.1295788641257399No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13320.1216342826042886No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC12280.11213731159013995No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGACCG1501.0499571E-625.3610975
GTAATAC2503.783498E-1022.8249873
ATAGACG1252.2453902E-422.8155888
GTACATG33100.022.753781
TACATGG31650.022.5644152
ACATGGG33550.021.11853
GTATAGG2951.2732926E-1021.0061191
GAGTACT21800.019.84153612-13
TACACTG10450.019.1118325
AGAGTAC35450.019.03980610-11
CCTAGAC3759.094947E-1219.0208243
CTAGACA5000.019.0208234
CTAATAC4500.019.0208233
GTCTAGG4051.8189894E-1218.8316961
CTACACT4900.018.4385534
GTACTTT23150.018.06643114-15
GTACCTG9100.017.8099211
CATGGGG21900.017.3706154
CCTATAC2202.8896682E-517.2916563
GTACGTG1952.2147405E-417.1114941
Produced by FastQC (version 0.11.5)