##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031594_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1095086 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.842082722270213 33.0 31.0 34.0 30.0 34.0 2 32.08973541804023 34.0 31.0 34.0 30.0 34.0 3 32.24180657957457 34.0 31.0 34.0 30.0 34.0 4 35.32183773694486 37.0 35.0 37.0 33.0 37.0 5 35.02453597251723 37.0 35.0 37.0 32.0 37.0 6 35.248107454574345 37.0 35.0 37.0 32.0 37.0 7 35.395264846779156 37.0 35.0 37.0 33.0 37.0 8 35.47206155498289 37.0 35.0 37.0 33.0 37.0 9 37.40002885618116 39.0 38.0 39.0 34.0 39.0 10-11 37.346790115114246 39.0 37.5 39.0 34.5 39.0 12-13 37.366711838157 39.0 38.0 39.0 34.5 39.0 14-15 38.75334631252706 41.0 39.0 41.0 35.0 41.0 16-17 38.71936176702104 41.0 38.0 41.0 34.0 41.0 18-19 38.65229580142564 41.0 38.5 41.0 34.0 41.0 20-21 38.641747314822766 40.5 39.0 41.0 34.0 41.0 22-23 38.575695424834215 40.0 38.0 41.0 34.0 41.0 24-25 38.48616318718347 40.0 38.0 41.0 34.0 41.0 26-27 38.38588521814725 40.0 38.0 41.0 34.0 41.0 28-29 38.28593781675595 40.0 38.0 41.0 34.0 41.0 30-31 38.151689912938345 40.0 38.0 41.0 33.0 41.0 32-33 37.988476247527586 40.0 38.0 41.0 33.0 41.0 34-35 37.83698631888272 40.0 38.0 41.0 33.0 41.0 36-37 37.698566140010925 40.0 37.5 41.0 32.5 41.0 38-39 37.52251832276187 40.0 37.0 41.0 31.0 41.0 40-41 37.39279974358178 40.0 37.0 41.0 31.0 41.0 42-43 37.19355009560893 40.0 37.0 41.0 31.0 41.0 44-45 36.97073106587062 40.0 36.5 41.0 30.0 41.0 46-47 36.756073495597605 40.0 36.0 41.0 30.0 41.0 48-49 36.88012630971449 40.0 36.5 41.0 30.0 41.0 50-51 36.63377944745892 39.5 36.0 40.5 30.0 41.0 52-53 36.775597076394 39.5 36.5 40.5 30.0 41.0 54-55 37.05101471482605 40.0 36.5 41.0 30.5 41.0 56-57 36.917687286660595 40.0 36.0 41.0 30.0 41.0 58-59 36.70306715636946 39.5 35.5 41.0 30.0 41.0 60-61 36.36958375871849 39.0 35.0 41.0 29.0 41.0 62-63 36.04288156363975 39.0 35.0 41.0 28.0 41.0 64-65 35.76444407105926 38.0 35.0 41.0 28.0 41.0 66-67 35.5035399046285 37.5 35.0 40.0 28.0 41.0 68-69 35.21620174123311 37.0 34.5 40.0 28.0 41.0 70-71 34.86577538202479 36.5 34.0 39.0 27.5 41.0 72-73 34.481121574013365 36.0 34.0 39.0 26.5 41.0 74-75 34.07654330344832 35.5 34.0 38.5 26.0 40.5 76-77 33.68090953587207 35.0 34.0 37.0 26.0 39.5 78-79 33.33303868371982 35.0 33.5 37.0 26.0 39.0 80-81 33.016290957970426 35.0 33.0 36.0 25.5 39.0 82-83 32.70493093693098 35.0 33.0 36.0 25.0 37.0 84-85 32.46915539053553 35.0 33.0 35.5 25.0 37.0 86-87 32.27881554508048 35.0 33.0 35.0 25.0 37.0 88-89 32.04268066617599 35.0 32.5 35.0 24.5 36.0 90-91 31.845360090440387 35.0 32.0 35.0 24.0 36.0 92-93 31.67197233824558 35.0 32.0 35.0 23.0 36.0 94-95 31.522617858323457 35.0 32.0 35.0 23.0 35.5 96-97 31.42023822786521 35.0 32.0 35.0 21.5 35.0 98-99 31.344451942587156 35.0 32.0 35.0 20.5 35.0 100-101 30.86825372619137 34.5 30.5 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.266493157403179 1101 2 0.09494533231238478 1101 3 0.23610782761537052 1101 4 0.23490728239491432 1101 5 0.28047015433571687 1101 6 0.18013060211878695 1101 7 0.164007122231979 1101 8 0.25837696302602353 1101 9 0.18631892690216745 1101 10-11 0.23869500891290585 1101 12-13 0.13146986564834862 1101 14-15 0.3435613276139975 1101 16-17 0.2711533313830401 1101 18-19 0.1882291583465161 1101 20-21 -0.25078835816133704 1101 22-23 0.07562167316787338 1101 24-25 0.25819746404412314 1101 26-27 0.0464029530464245 1101 28-29 0.1851318989487325 1101 30-31 0.11585465103898684 1101 32-33 0.20482121327754044 1101 34-35 0.1329230011812612 1101 36-37 0.12403532330726108 1101 38-39 0.1092062803879088 1101 40-41 -0.11501072431128279 1101 42-43 -0.07763655667297087 1101 44-45 0.09888060573305779 1101 46-47 0.15948322786140778 1101 48-49 0.22165650699334094 1101 50-51 -0.08494910761467622 1101 52-53 -0.1288756713349244 1101 54-55 -0.0021977863283453303 1101 56-57 0.02923251359538881 1101 58-59 0.18115680380516608 1101 60-61 0.05808201811860414 1101 62-63 -0.10021422219126919 1101 64-65 0.03430551965957562 1101 66-67 0.04336440611530179 1101 68-69 -0.09315765716090141 1101 70-71 0.073372628054301 1101 72-73 -0.009831389636495658 1101 74-75 -0.03446445363902484 1101 76-77 0.10541460091100419 1101 78-79 -0.025012018514395606 1101 80-81 0.21073707543288123 1101 82-83 0.1601450992900908 1101 84-85 0.14824362141352054 1101 86-87 0.01062214768052172 1101 88-89 -0.035930967467791675 1101 90-91 0.030132593229609483 1101 92-93 -0.015583673535765286 1101 94-95 -0.20598088496998557 1101 96-97 -0.060931684871377456 1101 98-99 -0.0266134353104448 1101 100-101 -0.3090885338461291 1102 1 0.1097243362692133 1102 2 0.28262806176837074 1102 3 0.20965338815972956 1102 4 0.2512789715111978 1102 5 0.3571577913828037 1102 6 0.41748746404493176 1102 7 0.3550290149610831 1102 8 0.4351742377467147 1102 9 0.43393039187031945 1102 10-11 0.5127293707520337 1102 12-13 0.43404591590764596 1102 14-15 0.4348118496981641 1102 16-17 0.45513035097859955 1102 18-19 0.45106155192836184 1102 20-21 0.3941998514380529 1102 22-23 0.31620566780781445 1102 24-25 0.49389779643770737 1102 26-27 0.36886932212511425 1102 28-29 0.33711712396699767 1102 30-31 0.5750711767429237 1102 32-33 0.6689159628511661 1102 34-35 0.46576688061462335 1102 36-37 0.5622259971438766 1102 38-39 0.6376816206127529 1102 40-41 0.7012200020818113 1102 42-43 0.44384394348365674 1102 44-45 0.18585873910816275 1102 46-47 -0.15568048902975562 1102 48-49 0.3133083812754762 1102 50-51 0.38042257280021374 1102 52-53 0.26621804277178285 1102 54-55 0.22610526612371018 1102 56-57 0.35714378539240954 1102 58-59 0.3184475350710443 1102 60-61 0.10312482901971975 1102 62-63 -0.241139964420924 1102 64-65 -0.10618297708609958 1102 66-67 -0.11714057978842618 1102 68-69 0.22190490592426926 1102 70-71 0.2448413813173218 1102 72-73 0.14743014842344593 1102 74-75 0.20828619026477924 1102 76-77 0.16910234237492716 1102 78-79 0.14059851540367418 1102 80-81 0.08595896120086621 1102 82-83 -0.1506874944240124 1102 84-85 0.1453003719617172 1102 86-87 0.1788311379697589 1102 88-89 0.01362134536503845 1102 90-91 0.09476987525947322 1102 92-93 -0.07582239651791411 1102 94-95 -0.015051444644207379 1102 96-97 -0.09858156653229955 1102 98-99 -0.220681906648025 1102 100-101 0.02328438216793316 1103 1 -0.0749061921840557 1103 2 0.03126710061405902 1103 3 -0.10047756952769049 1103 4 0.09036362969223433 1103 5 -0.17947878762103642 1103 6 -0.07566955310792878 1103 7 -4.882947313546993E-4 1103 8 0.028599732604156713 1103 9 0.2155265650828042 1103 10-11 0.22453217480664733 1103 12-13 0.15309256210314715 1103 14-15 0.2094634064973917 1103 16-17 0.20729870569109465 1103 18-19 0.14647349289215583 1103 20-21 -0.08033242779526262 1103 22-23 -0.07723334379802083 1103 24-25 0.13364085440083073 1103 26-27 0.13486293726717946 1103 28-29 -0.06687578541506412 1103 30-31 0.30270560132153435 1103 32-33 0.09725294880200863 1103 34-35 0.16996573984233265 1103 36-37 0.18245541041381586 1103 38-39 0.1662937910517286 1103 40-41 0.3199961069470163 1103 42-43 0.08998424114102477 1103 44-45 0.03048784365969226 1103 46-47 0.3151733773793737 1103 48-49 0.11206772949993393 1103 50-51 0.2909865823424127 1103 52-53 0.1771839434205873 1103 54-55 0.06752594860601135 1103 56-57 -0.07065459424869402 1103 58-59 0.19021383358688126 1103 60-61 0.1826466456707081 1103 62-63 0.498914391480227 1103 64-65 0.34983909545447744 1103 66-67 0.030797135860467506 1103 68-69 0.11077143886798524 1103 70-71 0.16756220990431814 1103 72-73 0.009031542608973098 1103 74-75 0.07166539799759875 1103 76-77 -0.4071847409790408 1103 78-79 -0.10386013366439784 1103 80-81 0.08845594830733461 1103 82-83 0.040246894660235455 1103 84-85 -0.02125176347816904 1103 86-87 -0.055583328871904314 1103 88-89 -0.07944095136782892 1103 90-91 0.06500568576910482 1103 92-93 -0.1124512369253523 1103 94-95 -0.22282811494285326 1103 96-97 -0.23199857988808859 1103 98-99 -0.03906685126030851 1103 100-101 0.04674625874660521 1104 1 0.009355279911659409 1104 2 0.10525366326906749 1104 3 0.07176394194816282 1104 4 -0.11260040211248423 1104 5 -0.14805283580910356 1104 6 -0.05113662860656376 1104 7 -0.01633955203997317 1104 8 0.07116334956467085 1104 9 0.04947765555191097 1104 10-11 0.1183065055697412 1104 12-13 0.11030498939268085 1104 14-15 0.21497812895759694 1104 16-17 0.041562432374895764 1104 18-19 0.18868389105242045 1104 20-21 -0.09840219978125475 1104 22-23 -0.001960162874844684 1104 24-25 -0.02341675784008146 1104 26-27 0.08448642105668824 1104 28-29 0.14552895823826617 1104 30-31 0.16949251268616194 1104 32-33 -0.14142526135566413 1104 34-35 0.012258806232431141 1104 36-37 0.08986699298434786 1104 38-39 -0.04602076907813313 1104 40-41 0.13006960651864574 1104 42-43 0.35039367433758173 1104 44-45 0.1464548364901006 1104 46-47 0.17236626490548446 1104 48-49 0.40978909247358075 1104 50-51 0.009396045600141179 1104 52-53 0.019544647453294317 1104 54-55 -0.05631256276463148 1104 56-57 -0.14489367076941306 1104 58-59 -0.10685219578288496 1104 60-61 0.06923485267195417 1104 62-63 0.3114995021804958 1104 64-65 0.2899286777544532 1104 66-67 0.02553793338342558 1104 68-69 -0.1053593873633929 1104 70-71 -0.05249009653930159 1104 72-73 -0.03380863715685223 1104 74-75 -0.13928588786914275 1104 76-77 -0.16190960607951865 1104 78-79 -0.2141966685128196 1104 80-81 -0.009510413238409399 1104 82-83 0.113599166533497 1104 84-85 0.22881211651762356 1104 86-87 0.22399160612080493 1104 88-89 0.2943111341261542 1104 90-91 0.24676757120597514 1104 92-93 0.14886003150590454 1104 94-95 0.2505707517508675 1104 96-97 0.08877519474328821 1104 98-99 0.09819247146142729 1104 100-101 0.33742729465467747 1105 1 0.15345722699008846 1105 2 0.21004735316889622 1105 3 0.34986098074649874 1105 4 0.1764189922783217 1105 5 -0.008207914542119 1105 6 0.049500301904025434 1105 7 0.2940098525684576 1105 8 0.2095854425546122 1105 9 0.4226540003156174 1105 10-11 0.3285322482574671 1105 12-13 0.23845932328941188 1105 14-15 0.4010878872677779 1105 16-17 0.3659133266558925 1105 18-19 0.36321621981169017 1105 20-21 0.26253018245343895 1105 22-23 0.3705295956263157 1105 24-25 0.23678367951250578 1105 26-27 0.4001547206336582 1105 28-29 0.33246615326123674 1105 30-31 0.22784327327859444 1105 32-33 0.2603040956978333 1105 34-35 0.12195886639229059 1105 36-37 0.27878325619803235 1105 38-39 0.18717266872325666 1105 40-41 0.35239129939781577 1105 42-43 0.42686544217784217 1105 44-45 0.16498836844644416 1105 46-47 0.5170258842750499 1105 48-49 0.4242709060544385 1105 50-51 0.46569659658800333 1105 52-53 0.26815906099021447 1105 54-55 0.3832707108863076 1105 56-57 0.48901404592542974 1105 58-59 0.42569714962061767 1105 60-61 0.3952215220123918 1105 62-63 0.31083487903900675 1105 64-65 0.27404026950777904 1105 66-67 0.23357309347547783 1105 68-69 0.2665325922777484 1105 70-71 0.2849665740425351 1105 72-73 0.2353059625891447 1105 74-75 0.3315585463895019 1105 76-77 0.5282418439639258 1105 78-79 0.5863571841050899 1105 80-81 0.5155709935472359 1105 82-83 0.37287906263838266 1105 84-85 0.1800754734374337 1105 86-87 0.23533699052317303 1105 88-89 0.3183454729884616 1105 90-91 0.32003320083135733 1105 92-93 0.383611895983897 1105 94-95 0.3066846162097612 1105 96-97 0.35849282539998484 1105 98-99 0.31452682655734776 1105 100-101 0.3318620307630056 1106 1 0.24308746882544696 1106 2 0.21725456527461517 1106 3 0.2315006690773913 1106 4 0.26847157511187447 1106 5 0.23761936973541253 1106 6 0.26909507406985256 1106 7 0.26757797082727564 1106 8 0.35605052840136864 1106 9 0.22370848441045155 1106 10-11 0.23736278225995022 1106 12-13 0.2935474174788766 1106 14-15 0.4660570121619685 1106 16-17 0.304458997706881 1106 18-19 0.4477652547720652 1106 20-21 0.2804979171975859 1106 22-23 0.2552241704162057 1106 24-25 0.2997070001405504 1106 26-27 0.06974849595133747 1106 28-29 0.15344264758191883 1106 30-31 0.2211802496387918 1106 32-33 0.24924179993843154 1106 34-35 0.4062971132807647 1106 36-37 0.09625854795735478 1106 38-39 0.22501075883470634 1106 40-41 0.25029003412565487 1106 42-43 0.07927899278782746 1106 44-45 0.29395106638876456 1106 46-47 0.4793694923941061 1106 48-49 0.28289382377459305 1106 50-51 0.12786883744826838 1106 52-53 0.20636127254314118 1106 54-55 0.1583971746671935 1106 56-57 0.2137113212675672 1106 58-59 0.1384834489396809 1106 60-61 0.3838896241403873 1106 62-63 0.24567000941767958 1106 64-65 0.5439799511326058 1106 66-67 0.33408302661415235 1106 68-69 0.41798282508710827 1106 70-71 0.38513621947271304 1106 72-73 0.19996724265090648 1106 74-75 0.2085556410302658 1106 76-77 0.36312527616647117 1106 78-79 0.25696593208194685 1106 80-81 0.21498185629211974 1106 82-83 0.35134668954168546 1106 84-85 0.4454414345168587 1106 86-87 0.38620180160231854 1106 88-89 0.45470886219966644 1106 90-91 0.506166046252833 1106 92-93 0.5189444535881265 1106 94-95 0.34937418500950557 1106 96-97 0.5697738840164668 1106 98-99 0.4002163464209083 1106 100-101 0.47024902171343896 1107 1 0.19097706976642925 1107 2 0.19789624612296564 1107 3 0.1783982039408727 1107 4 0.43994871980323325 1107 5 0.21187429944379232 1107 6 0.14110638814042176 1107 7 0.09185544354328812 1107 8 0.22938054326498047 1107 9 0.35101870833766213 1107 10-11 0.4169471569586207 1107 12-13 0.3670497842775049 1107 14-15 0.3900809954897042 1107 16-17 0.3038100718225465 1107 18-19 0.4896670159065266 1107 20-21 0.4402861432322922 1107 22-23 0.45887961248936193 1107 24-25 0.4354476139532082 1107 26-27 0.38286435658603324 1107 28-29 0.4517439733192603 1107 30-31 0.4116721389339446 1107 32-33 0.31827943503359535 1107 34-35 0.5376003597344763 1107 36-37 0.433541902704377 1107 38-39 0.49101347094529757 1107 40-41 0.5039614170153826 1107 42-43 0.4187084939836012 1107 44-45 0.295851368091526 1107 46-47 0.5092865393382908 1107 48-49 0.37584702727061625 1107 50-51 0.2108840059822228 1107 52-53 0.28442219165501115 1107 54-55 0.30548791049006496 1107 56-57 0.32068505931521685 1107 58-59 0.3580436561442326 1107 60-61 0.44928172044898673 1107 62-63 0.48265522311584164 1107 64-65 0.49529009513855016 1107 66-67 0.32458455858309065 1107 68-69 0.5367236656328984 1107 70-71 0.4425283713786996 1107 72-73 0.44792208366407493 1107 74-75 0.5622688957106519 1107 76-77 0.3997105360799651 1107 78-79 0.3675065907199979 1107 80-81 0.44962720135649903 1107 82-83 0.4733647361097155 1107 84-85 0.5440299555652572 1107 86-87 0.31910573190403113 1107 88-89 0.47294784935405687 1107 90-91 0.6567754892972069 1107 92-93 0.7266376567193049 1107 94-95 0.7456624505704745 1107 96-97 0.6598672963328518 1107 98-99 0.5057727851643037 1107 100-101 0.4653017219661457 1108 1 0.13961717117864936 1108 2 0.18996842580359186 1108 3 0.13020411000481147 1108 4 0.1356762975717345 1108 5 0.26799621369923443 1108 6 0.44686522469474 1108 7 0.3680207441283727 1108 8 0.2938752871641981 1108 9 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-1.0304969132182649 2316 48-49 -0.8502498985251279 2316 50-51 -1.6492639663909756 2316 52-53 -1.4847705681685142 2316 54-55 -0.9930621746414019 2316 56-57 -1.2074376944657743 2316 58-59 -1.6980113243221524 2316 60-61 -1.1287727152347244 2316 62-63 -0.8526093232057406 2316 64-65 -1.438707868167178 2316 66-67 -1.5003434339221116 2316 68-69 -1.6994829435648242 2316 70-71 -1.1912877284912682 2316 72-73 -0.9444227652395014 2316 74-75 -1.0663747055524766 2316 76-77 -0.8399865001407392 2316 78-79 -1.1198841131729935 2316 80-81 -1.1729573445832884 2316 82-83 -1.2814511207372519 2316 84-85 -1.737686197975357 2316 86-87 -1.1873384157570364 2316 88-89 -1.0725627963015185 2316 90-91 -1.3675308489041704 2316 92-93 -1.4210017529477241 2316 94-95 -1.29338385669136 2316 96-97 -1.2087086549626918 2316 98-99 -0.7264929601196926 2316 100-101 -1.2399686466285296 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 6.0 7 70.0 8 328.0 9 359.0 10 351.0 11 471.0 12 700.0 13 981.0 14 1400.0 15 1733.0 16 2194.0 17 2919.0 18 3736.0 19 4745.0 20 5659.0 21 6907.0 22 7996.0 23 8415.0 24 8725.0 25 9401.0 26 10019.0 27 11585.0 28 13387.0 29 15708.0 30 18382.0 31 22376.0 32 28216.0 33 36567.0 34 51194.0 35 76788.0 36 132234.0 37 236739.0 38 288296.0 39 86376.0 40 123.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.00589506328591 18.345253441845315 13.369820028534859 23.27903146633391 2 15.209421348530869 20.07215041601974 39.923688470048226 24.794739765401165 3 19.322832930867726 24.489795918367346 29.848094014990206 26.339277135774726 4 12.225217267938621 16.413694630427123 38.67419648618593 32.68689161544833 5 13.28361529369867 38.184534735643275 35.052957402455206 13.478892568202856 6 31.608762607846675 37.20677005794828 17.40031531626331 13.784152017941734 7 27.830485181589687 32.3843865715219 21.714088080583313 18.0710401663051 8 25.80352666039607 35.213901688302656 19.668652761562477 19.313918889738794 9 25.58706387090222 16.48966685240439 20.348711135754147 37.574558140939246 10-11 25.00490713953343 25.801899806505425 28.5340212327009 20.659171821260244 12-13 25.968120906690384 23.414142744994702 28.263622406312507 22.354113942002414 14-15 22.615012040067366 25.26030383154612 26.369417542581758 25.755266585804755 16-17 22.339701141672084 28.368508459487597 26.73602066361575 22.555769735224573 18-19 22.226728950874676 28.28779177451984 27.787636246705027 21.697843027900458 20-21 23.36399611171558 26.59886063586874 27.633712284303176 22.403430968112506 22-23 22.756614548994325 26.631607015339437 27.589750941935154 23.022027493731088 24-25 23.07321068847561 26.75967914848697 27.569889488131526 22.5972206749059 26-27 22.931395342466253 26.468240850490282 28.13418306872702 22.466180738316442 28-29 22.51471573922048 27.52071526802461 27.56011856603043 22.404450426724477 30-31 22.85144728359234 26.680233333272458 27.53368228614008 22.93463709699512 32-33 22.807249841564957 26.966968804276558 27.638879503527576 22.58690185063091 34-35 23.270364154048178 26.75566119921175 27.524961509872288 22.44901313686779 36-37 23.123389395901324 26.523989896683915 27.917122490836338 22.435498216578424 38-39 22.80989803540544 27.15681690753055 27.65696027526605 22.37632478179796 40-41 22.94454499463969 27.139010086879022 27.371320608609732 22.545124309871554 42-43 22.703422379612196 27.0442230107955 27.192887133978516 23.059467475613786 44-45 23.00212195600359 26.82488902138096 27.078005772854237 23.094983249761217 46-47 23.424168626709996 27.097825873120957 27.07545369989537 22.40255180027368 48-49 23.158099172420727 27.075688545041608 27.133678500760862 22.632533781776807 50-51 22.410857342050956 27.319066000311583 27.6265828712509 22.643493786386564 52-53 22.982419908142695 27.626002233378966 26.66466235221227 22.72691550626607 54-55 22.75382338386093 27.109389743657697 27.056521057684442 23.080265814796928 56-57 23.115088162613787 26.96856278529316 26.968151387188698 22.94819766490436 58-59 23.639266914733273 26.866783479166152 26.71893980317368 22.775009802926892 60-61 23.011812535662553 27.05795805351944 27.53894533936122 22.391284071456788 62-63 22.583765997514995 27.233519670679442 27.42728610295615 22.755428228849414 64-65 22.61922014701734 27.764004204152858 26.909732778448554 22.70704287038125 66-67 22.863350994007074 27.143230623535132 26.664829298436132 23.32858908402166 68-69 22.723863285758362 27.07536195449715 26.961821942384383 23.238952817360104 70-71 23.222107518744423 26.941653402648964 26.83842027262869 22.997818805977925 72-73 22.90423689705432 26.685734911794135 27.419298830775418 22.990729360376125 74-75 22.512866846881778 27.766875048010625 26.832588330400874 22.887669774706723 76-77 22.800913002970738 26.904438534289778 26.809898431165102 23.484750031574382 78-79 22.830279002502387 26.951922465825955 26.995953112619237 23.22184541905242 80-81 23.2099805703862 26.880495994355723 26.775031392626254 23.134492042631827 82-83 22.906428028907804 26.78194569230024 27.372568661519665 22.939057617272294 84-85 22.75651514182031 27.180778617720453 27.058885364582856 23.00382087587638 86-87 22.70338413062788 27.27852184624832 26.943234114727073 23.07485990839672 88-89 22.622661920315966 27.161279796276805 26.484975094455514 23.73108318895171 90-91 22.96973545089639 26.701526643929512 26.67917347204185 23.649564433132248 92-93 23.110404756460095 26.999817059227077 26.87386233706837 23.015915847244457 94-95 22.914158716282973 26.86421200332295 27.059483515765052 23.16214576462902 96-97 22.190929339218 26.96411712180218 27.588441364611388 23.25651217436843 98-99 22.70687087707851 27.174533373360255 26.578927287623138 23.5396684619381 100-101 22.566881713356057 26.754421432118665 26.821763437676893 23.856933416848385 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 39.0 1 28.5 2 33.0 3 59.5 4 53.0 5 42.0 6 79.0 7 163.5 8 290.5 9 366.0 10 338.5 11 351.0 12 418.5 13 447.5 14 474.0 15 540.0 16 590.0 17 655.0 18 766.0 19 834.5 20 922.0 21 1124.0 22 1417.0 23 1809.0 24 2328.5 25 2918.0 26 3889.5 27 5319.0 28 7149.0 29 9260.0 30 11686.5 31 14327.0 32 16844.0 33 19202.5 34 21415.0 35 23457.0 36 25613.5 37 28062.0 38 29849.5 39 30632.5 40 31758.5 41 33141.0 42 33815.0 43 34988.5 44 36429.0 45 37841.0 46 39614.5 47 44889.5 48 54334.5 49 53816.5 50 45548.0 51 41729.5 52 39940.0 53 38471.0 54 36870.0 55 34830.0 56 32488.0 57 29829.5 58 27009.5 59 23591.0 60 19478.0 61 15769.5 62 12248.0 63 8964.5 64 6511.0 65 4661.0 66 3320.5 67 2453.0 68 1786.5 69 1295.0 70 983.0 71 752.0 72 544.0 73 424.0 74 332.5 75 237.0 76 162.5 77 108.0 78 72.5 79 53.0 80 38.0 81 32.0 82 25.0 83 21.5 84 21.0 85 15.0 86 13.0 87 10.5 88 6.0 89 7.5 90 5.5 91 2.5 92 3.0 93 4.5 94 4.0 95 3.5 96 3.5 97 2.5 98 2.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47402669744659326 2 0.36819026085622497 3 0.20445882789114278 4 0.2320365706437668 5 0.25504846194728087 6 0.20226721919557 7 0.19678819745663811 8 0.1966968804276559 9 0.19605766122478052 10-11 0.209161654883726 12-13 0.19578371013783394 14-15 0.20788321647797525 16-17 0.09031254166339447 18-19 0.03894671286090773 20-21 1.3697554347329798E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13875622553845085 46-47 0.20185629256515014 48-49 0.08501615398242694 50-51 0.06159333604849299 52-53 0.07122728260611495 54-55 0.07903488858409294 56-57 0.11460287137265932 58-59 0.09359995470675363 60-61 0.1371581775312624 62-63 0.2313516929264003 64-65 0.1283460842344802 66-67 0.1031882427498845 68-69 0.10944345923516509 70-71 0.08771000633740181 72-73 0.07040542934527516 74-75 0.1440982717339095 76-77 0.22171774636877833 78-79 0.13966939582827284 80-81 0.08035898550433482 82-83 0.09012990760543008 84-85 0.10008346376448973 86-87 0.07323625724372332 88-89 0.09611117300376408 90-91 0.11702277264068758 92-93 0.16765806521131674 94-95 0.13519486140814513 96-97 0.08611195833021333 98-99 0.07510825633785839 100-101 0.0614107019905286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1095086.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.73136102012461 #Duplication Level Percentage of deduplicated Percentage of total 1 74.80545305669746 46.92647881973672 2 14.384632991831664 18.047352107051744 3 5.1234878853728665 9.642101046586804 4 2.3121151826002553 5.801685289592317 5 1.226572063825889 3.847226747653059 6 0.6877332804483406 2.5885466820815677 7 0.42835315430096754 1.8809823456594197 8 0.27458677432070133 1.3780161657010717 9 0.1755654879141515 0.9912115804515266 >10 0.5657053467467511 5.2594022297487335 >50 0.008633285391569549 0.37480383518881555 >100 0.004238437383578164 0.5321320312748175 >500 0.0013153739245777179 0.6019942005289031 >1k 0.001461526582864131 1.5975858885495182 >5k 1.461526582864131E-4 0.5304810301949792 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5786 0.5283603296910014 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3313 0.30253331701802416 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2357 0.21523423731104224 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1852 0.16911913767503192 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1658 0.1514036340524854 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1638 0.1495772934728414 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1548 0.14135876086444352 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1419 0.1295788641257399 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1332 0.1216342826042886 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1228 0.11213731159013995 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 4.565851449109933E-5 0.0 18-19 0.0 0.0 0.0 1.8263405796439732E-4 0.0 20-21 0.0 0.0 0.0 4.565851449109933E-4 0.0 22-23 4.565851449109933E-5 0.0 0.0 0.0010501458332952845 0.0 24-25 1.8263405796439732E-4 0.0 0.0 0.0017350235506617745 0.0 26-27 1.8263405796439732E-4 0.0 0.0 0.0028308278984481585 0.0 28-29 1.8263405796439732E-4 0.0 0.0 0.004520192934618833 0.0 30-31 1.8263405796439732E-4 0.0 0.0 0.0078076059779779855 0.0 32-33 1.8263405796439732E-4 0.0 0.0 0.015569553441464871 0.0 34-35 1.8263405796439732E-4 0.0 0.0 0.025614426629506724 0.0 36-37 1.8263405796439732E-4 0.0 0.0 0.04164056521588259 0.0 38-39 1.8263405796439732E-4 0.0 0.0 0.06899001539605108 0.0 40-41 1.8263405796439732E-4 0.0 0.0 0.1012705851412583 0.0 42-43 1.8263405796439732E-4 0.0 0.0 0.13204442390825927 0.0 44-45 1.8263405796439732E-4 0.0 0.0 0.1695300643054518 0.0 46-47 1.8263405796439732E-4 0.0 0.0 0.21368184781834487 0.0 48-49 1.8263405796439732E-4 0.0 0.0 0.2569204610414159 0.0 50-51 1.8263405796439732E-4 0.0 0.0 0.3036747798803016 0.0 52-53 1.8263405796439732E-4 0.0 0.0 0.3533055851321266 0.0 54-55 1.8263405796439732E-4 0.0 0.0 0.4087350217243212 0.0 56-57 1.8263405796439732E-4 0.0 0.0 0.47950571918552515 0.0 58-59 1.8263405796439732E-4 0.0 0.0 0.5502764166467291 0.0 60-61 1.8263405796439732E-4 0.0 0.0 0.626480477332374 0.0 62-63 1.8263405796439732E-4 0.0 0.0 0.7091223885612636 0.0 64-65 1.8263405796439732E-4 0.0 0.0 0.7950517128335126 0.0 66-67 1.8263405796439732E-4 0.0 0.0 0.8912085443517679 0.0 68-69 1.8263405796439732E-4 0.0 0.0 1.0125232173546188 0.0 70-71 1.8263405796439732E-4 0.0 0.0 1.139499546154366 0.0 72-73 1.8263405796439732E-4 0.0 0.0 1.2703111901713657 0.0 74-75 1.8263405796439732E-4 0.0 0.0 1.4045928812896888 0.0 76-77 1.8263405796439732E-4 0.0 0.0 1.5388289138935207 0.0 78-79 1.8263405796439732E-4 0.0 0.0 1.7190887291043808 0.0 80-81 1.8263405796439732E-4 0.0 0.0 1.9124525379741866 0.0 82-83 1.8263405796439732E-4 0.0 0.0 2.1115236611553794 0.0 84-85 1.8263405796439732E-4 0.0 0.0 2.3179001466551483 0.0 86-87 1.8263405796439732E-4 0.0 0.0 2.5311710678430734 0.0 88-89 1.8263405796439732E-4 0.0 0.0 2.7869500660222117 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGGACCG 150 1.0499571E-6 25.361097 5 GTAATAC 250 3.783498E-10 22.824987 3 ATAGACG 125 2.2453902E-4 22.815588 8 GTACATG 3310 0.0 22.75378 1 TACATGG 3165 0.0 22.564415 2 ACATGGG 3355 0.0 21.1185 3 GTATAGG 295 1.2732926E-10 21.006119 1 GAGTACT 2180 0.0 19.841536 12-13 TACACTG 1045 0.0 19.111832 5 AGAGTAC 3545 0.0 19.039806 10-11 CCTAGAC 375 9.094947E-12 19.020824 3 CTAGACA 500 0.0 19.020823 4 CTAATAC 450 0.0 19.020823 3 GTCTAGG 405 1.8189894E-12 18.831696 1 CTACACT 490 0.0 18.438553 4 GTACTTT 2315 0.0 18.066431 14-15 GTACCTG 910 0.0 17.809921 1 CATGGGG 2190 0.0 17.370615 4 CCTATAC 220 2.8896682E-5 17.291656 3 GTACGTG 195 2.2147405E-4 17.111494 1 >>END_MODULE