FastQCFastQC Report
Fri 10 Feb 2017
SRR2031594_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031594_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1095086
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT63420.5791325978051038No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT30910.28226093658397605No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT21920.20016692752897947No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17720.16181377535645602No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC17440.15925689854495445No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT15450.14108480977749693No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC15400.14062822463258592No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14480.13222705796622367No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT13530.1235519402129148No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC12470.11387233514080172No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG4100.025.50471
GTACATG29900.024.9580021
TACATGG29000.023.7505472
ACATGGG28650.023.2117043
TCTATAC4400.021.5914063
GTATTAC3102.564775E-1019.9325561
GTACGGG1200.004745133719.8047851
AGAGTAC34850.019.76192310-11
GGGTATA2201.3361205E-619.4446961
GTGTACG2201.3361205E-619.4446961
TATACTG5500.018.1351265
CATGGGG17500.017.9146984
GGATCGT1600.00119151117.8112856
CTATACT3751.70985E-1017.7337424
GTACCTG8600.017.6861321
ATTATAC5650.017.6552753
TATACTC3551.5843398E-917.3931795
AGTACTT23950.016.85688612-13
GTACTAA2858.522238E-716.6777131
GAGTACT21650.016.56353612-13
Produced by FastQC (version 0.11.5)