##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031594_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1095086 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.47270990588867 34.0 31.0 34.0 31.0 34.0 2 32.69828853624281 34.0 31.0 34.0 31.0 34.0 3 32.814405443956 34.0 31.0 34.0 31.0 34.0 4 36.25104421022641 37.0 37.0 37.0 35.0 37.0 5 35.916188317629846 37.0 35.0 37.0 35.0 37.0 6 35.97993947507319 37.0 36.0 37.0 35.0 37.0 7 35.948873421813445 37.0 36.0 37.0 35.0 37.0 8 36.07243540689955 37.0 37.0 37.0 35.0 37.0 9 37.98203611405862 39.0 39.0 39.0 35.0 39.0 10-11 37.9571809885251 39.0 38.5 39.0 35.0 39.0 12-13 37.97638450313491 39.0 38.0 39.0 35.0 39.0 14-15 39.42518898059148 41.0 39.0 41.0 36.0 41.0 16-17 39.404405681380275 41.0 39.0 41.0 36.0 41.0 18-19 39.41374376076399 41.0 39.0 41.0 36.0 41.0 20-21 39.42442922291035 41.0 39.0 41.0 36.0 41.0 22-23 39.3617282113003 41.0 39.0 41.0 36.0 41.0 24-25 39.286340068268615 41.0 39.0 41.0 36.0 41.0 26-27 39.15745567014828 41.0 39.0 41.0 36.0 41.0 28-29 39.090670504417005 40.5 39.0 41.0 36.0 41.0 30-31 39.00014473749094 40.0 39.0 41.0 35.0 41.0 32-33 38.849275308058 40.0 38.0 41.0 35.0 41.0 34-35 38.71618073831644 40.0 38.0 41.0 35.0 41.0 36-37 38.57925907189025 40.0 38.0 41.0 34.5 41.0 38-39 38.46692725502837 40.0 38.0 41.0 34.0 41.0 40-41 38.37135211298474 40.0 38.0 41.0 34.0 41.0 42-43 38.21750848791784 40.0 38.0 41.0 34.0 41.0 44-45 38.13913427803844 40.0 38.0 41.0 33.0 41.0 46-47 38.14326728677017 40.0 38.0 41.0 33.0 41.0 48-49 38.231993651640146 40.0 38.0 41.0 34.0 41.0 50-51 38.131166410674595 40.0 38.0 41.0 33.0 41.0 52-53 38.05117086694561 40.0 38.0 41.0 33.0 41.0 54-55 37.934895067602 40.0 37.0 41.0 33.0 41.0 56-57 37.7941193659676 40.0 37.0 41.0 33.0 41.0 58-59 37.575848380857764 40.0 36.5 41.0 33.0 41.0 60-61 37.24399636192957 39.0 36.0 41.0 32.0 41.0 62-63 37.06178236229849 39.0 35.5 41.0 32.0 41.0 64-65 36.75085792348729 39.0 35.0 41.0 31.0 41.0 66-67 36.42184632074559 38.0 35.0 40.0 31.0 41.0 68-69 36.08448331911832 37.0 35.0 40.0 31.0 41.0 70-71 35.70572493849798 37.0 35.0 39.0 31.0 41.0 72-73 35.31059752384745 36.0 35.0 39.0 30.0 41.0 74-75 34.95190332083507 36.0 34.0 38.5 30.0 40.5 76-77 34.353800066844066 35.0 34.0 37.0 29.5 39.0 78-79 34.30816255526963 35.0 34.0 37.0 30.0 39.0 80-81 34.04227339222673 35.0 34.0 36.5 29.5 39.0 82-83 33.78498492355851 35.0 34.0 36.0 29.0 37.5 84-85 33.540962536275686 35.0 34.0 36.0 29.0 37.0 86-87 33.3489835501504 35.0 34.0 35.0 29.0 37.0 88-89 33.13349545149879 35.0 34.0 35.0 29.0 36.0 90-91 32.98050427089744 35.0 34.0 35.0 29.0 36.0 92-93 32.87076768399925 35.0 34.0 35.0 29.0 36.0 94-95 32.749858458605075 35.0 33.5 35.0 28.5 36.0 96-97 32.65472163830056 35.0 33.0 35.0 27.0 35.0 98-99 32.59236991432636 35.0 33.0 35.0 27.0 35.0 100-101 32.144925147431344 34.5 32.5 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18760059670022855 1101 2 0.25480435553349423 1101 3 0.2143083024392709 1101 4 0.15595352337960833 1101 5 0.21898126217028135 1101 6 0.1767660663856958 1101 7 0.13773721486328583 1101 8 0.0989331874684467 1101 9 0.09130296851993336 1101 10-11 0.02728130469520096 1101 12-13 0.027035306731377773 1101 14-15 0.1957024220646133 1101 16-17 0.18774694993762608 1101 18-19 0.11163368946177599 1101 20-21 0.0948223566809574 1101 22-23 0.1878033965982766 1101 24-25 0.10986207926965363 1101 26-27 0.11223618275240455 1101 28-29 0.20983168570832333 1101 30-31 0.1462413752176559 1101 32-33 0.13798193143620097 1101 34-35 -0.01551331149755697 1101 36-37 0.055415063776145246 1101 38-39 0.15704204640679365 1101 40-41 0.19280559297549615 1101 42-43 0.12488131811130643 1101 44-45 0.08574595084278513 1101 46-47 0.24398563431478948 1101 48-49 0.06783347287539954 1101 50-51 0.15823369271205934 1101 52-53 0.06016948473858719 1101 54-55 0.16647191491079383 1101 56-57 0.22392401263255834 1101 58-59 0.17287085846274408 1101 60-61 0.3055555968615309 1101 62-63 -0.014108079510052107 1101 64-65 0.014893047439528573 1101 66-67 0.26934604438761767 1101 68-69 0.1352759217820534 1101 70-71 0.19726724724170452 1101 72-73 0.11382535533652316 1101 74-75 -0.050977889176216706 1101 76-77 0.06876396429897369 1101 78-79 0.19643156767502035 1101 80-81 0.2509121783693473 1101 82-83 0.13007949279927544 1101 84-85 0.04180740268700589 1101 86-87 0.07551238638254887 1101 88-89 0.04794865765259004 1101 90-91 0.12930272731510684 1101 92-93 -0.14030602673823722 1101 94-95 0.006282391948374766 1101 96-97 -0.0862882870730175 1101 98-99 -0.2994254933485152 1101 100-101 -0.15672679444197257 1102 1 0.048117111962490355 1102 2 0.1799831693582874 1102 3 0.17272212374727047 1102 4 0.17428085274509897 1102 5 -0.22965870859300708 1102 6 -0.12954071026669567 1102 7 -0.017230415918795927 1102 8 -0.03250149980313921 1102 9 0.10367636311114126 1102 10-11 0.060049699460442696 1102 12-13 -0.013681819369352866 1102 14-15 0.1059594179923593 1102 16-17 0.25680780754787236 1102 18-19 0.19643647217035465 1102 20-21 0.10973656085067063 1102 22-23 0.04846892338119346 1102 24-25 0.10659905326986063 1102 26-27 0.11811398025336217 1102 28-29 -0.044547295183392066 1102 30-31 -0.11519988161294492 1102 32-33 0.13058791452067453 1102 34-35 0.10272678630620646 1102 36-37 0.16078252401073456 1102 38-39 0.1302373754938415 1102 40-41 0.06606966314361529 1102 42-43 0.12286259269276911 1102 44-45 0.15909659996742676 1102 46-47 0.33032421459393646 1102 48-49 0.21666226407653966 1102 50-51 0.22957649317149276 1102 52-53 0.015231090669466596 1102 54-55 -0.10820417537496496 1102 56-57 -0.06413157192767471 1102 58-59 -0.13550467667351995 1102 60-61 0.07524143271826489 1102 62-63 0.07233709295174151 1102 64-65 0.04616915164753266 1102 66-67 0.08780106683723687 1102 68-69 0.04196425494135525 1102 70-71 0.19811563400165255 1102 72-73 0.20062293460629377 1102 74-75 0.31340204886979706 1102 76-77 0.07654410595780092 1102 78-79 0.010476066648259064 1102 80-81 0.2880660800783019 1102 82-83 -0.17467734650322342 1102 84-85 -0.06996646526050654 1102 86-87 -0.06765505470895761 1102 88-89 0.1935307379839415 1102 90-91 0.2327743694438169 1102 92-93 0.1790887471993159 1102 94-95 0.19809176161876252 1102 96-97 0.1505997867844897 1102 98-99 0.0769232240647284 1102 100-101 0.17337614315156458 1103 1 0.09323622443834978 1103 2 0.08910625225000501 1103 3 0.07595768249456114 1103 4 0.0973746816787866 1103 5 -0.007915935410238717 1103 6 0.15065013383875936 1103 7 0.099597626278225 1103 8 0.013917696868645635 1103 9 0.08487260566145238 1103 10-11 0.14759644058272414 1103 12-13 0.08785114498770952 1103 14-15 0.15276812401649664 1103 16-17 0.0939454348735822 1103 18-19 0.08232983637552849 1103 20-21 -0.028204435963097296 1103 22-23 -0.0173684752947878 1103 24-25 3.1820206552879426E-4 1103 26-27 -0.0017063192200055255 1103 28-29 0.09587314791831858 1103 30-31 0.049061113384830435 1103 32-33 0.12909488132106617 1103 34-35 0.05458085082339892 1103 36-37 0.1296929709095167 1103 38-39 0.028399510438454456 1103 40-41 0.039440637009853674 1103 42-43 -0.18751084101531745 1103 44-45 -0.17217413947093974 1103 46-47 -0.13476594521323904 1103 48-49 -0.23414502630036083 1103 50-51 0.04941784162707563 1103 52-53 -0.1402734245435866 1103 54-55 0.017050166493525865 1103 56-57 -0.15015793680971257 1103 58-59 -0.11032328397379842 1103 60-61 0.06267549987690302 1103 62-63 -0.25599555938511287 1103 64-65 -0.14443402551060558 1103 66-67 -0.19745339884879343 1103 68-69 -0.03376564947149774 1103 70-71 -0.02227752796820681 1103 72-73 -0.15297056002002307 1103 74-75 -0.2871790681295323 1103 76-77 -0.48900025786766577 1103 78-79 -0.13588349124056265 1103 80-81 0.04083494987177971 1103 82-83 0.1498035484499738 1103 84-85 -0.024543999805601402 1103 86-87 -0.10207787923473433 1103 88-89 -0.05816195100289434 1103 90-91 0.01624379889258165 1103 92-93 0.006566026680275172 1103 94-95 -0.04703189083365089 1103 96-97 -0.07641503417534068 1103 98-99 -0.14839374884399348 1103 100-101 0.11041389099301568 1104 1 -0.054060524445631586 1104 2 0.0327205072589436 1104 3 0.11983797889774195 1104 4 0.062272248226705074 1104 5 -0.06960447158134286 1104 6 -0.12078122898458332 1104 7 -0.07123702223149309 1104 8 -0.041004068924749504 1104 9 0.10979331931373082 1104 10-11 0.08794454898331594 1104 12-13 0.0072631484146796765 1104 14-15 2.497491235899929E-4 1104 16-17 0.19810439836428628 1104 18-19 0.05819071325998948 1104 20-21 -0.011322080369460252 1104 22-23 0.01953011333307586 1104 24-25 0.06928154246323714 1104 26-27 0.027071243119358712 1104 28-29 0.009868127702176821 1104 30-31 0.07559460904850113 1104 32-33 0.11768215642590008 1104 34-35 0.0988369119077106 1104 36-37 0.07226710928110691 1104 38-39 0.11270006206100192 1104 40-41 0.03318490659847129 1104 42-43 0.20835725055102472 1104 44-45 0.18466853537236716 1104 46-47 0.21368966194733474 1104 48-49 0.31277595790249535 1104 50-51 0.2706049574076914 1104 52-53 0.2015311477610524 1104 54-55 0.13115486956427702 1104 56-57 0.2169160587712966 1104 58-59 0.3101288360905343 1104 60-61 0.20375409481760443 1104 62-63 0.1754980300694342 1104 64-65 0.16359381113696259 1104 66-67 0.18900253898470254 1104 68-69 0.10255576460614435 1104 70-71 0.11250475397554993 1104 72-73 0.13837933702046 1104 74-75 -0.10084638111144528 1104 76-77 -0.07464003481374704 1104 78-79 0.1319149786456535 1104 80-81 0.04703506711035743 1104 82-83 0.008001982262783258 1104 84-85 0.08593231950419522 1104 86-87 -0.006301474250591355 1104 88-89 0.011324453830944492 1104 90-91 -0.13075684742266702 1104 92-93 -0.02662601913294793 1104 94-95 0.04039051375969649 1104 96-97 -0.07502771097262695 1104 98-99 -0.0947450737902571 1104 100-101 -0.013607993542017027 1105 1 0.07431200828419549 1105 2 0.07329403119397426 1105 3 0.026524316881527454 1105 4 0.1122803650323192 1105 5 0.1841144577642524 1105 6 0.14843984415261247 1105 7 0.19478698441474052 1105 8 0.14838487885077 1105 9 0.20493183021412165 1105 10-11 0.1918176976354502 1105 12-13 0.16842089136977023 1105 14-15 0.045061200503916155 1105 16-17 0.005358435281429763 1105 18-19 0.03476656719568183 1105 20-21 0.10754185332101684 1105 22-23 0.01570662043289417 1105 24-25 0.14611952147412666 1105 26-27 -0.0055330100867792 1105 28-29 -0.005319847966504199 1105 30-31 0.0035670310078117495 1105 32-33 0.11721711121052181 1105 34-35 0.11323326030010605 1105 36-37 -0.06663912729035815 1105 38-39 0.036907241136837854 1105 40-41 0.08782008005938025 1105 42-43 0.1347879569040913 1105 44-45 0.10663404372313323 1105 46-47 0.12940522998048465 1105 48-49 0.15414063165464853 1105 50-51 0.3142418592707372 1105 52-53 0.2676893204293833 1105 54-55 0.2375065086815482 1105 56-57 0.24663980090515025 1105 58-59 0.170120146333943 1105 60-61 0.21893872827221372 1105 62-63 0.2960999150781731 1105 64-65 0.13114425469652247 1105 66-67 0.2495653678346912 1105 68-69 0.32797043788085034 1105 70-71 0.23358887272918594 1105 72-73 0.23286802601311507 1105 74-75 0.26548033463802057 1105 76-77 0.3568226135566803 1105 78-79 0.22434008917744563 1105 80-81 0.22300263808466525 1105 82-83 0.25152954597971444 1105 84-85 0.11468395480179083 1105 86-87 0.26428560952439284 1105 88-89 0.4262316835174431 1105 90-91 0.18134721957439837 1105 92-93 0.2975095410698714 1105 94-95 0.21037504408312913 1105 96-97 0.1601347683772616 1105 98-99 0.22943966470536026 1105 100-101 0.32170966003272383 1106 1 0.1577472919962375 1106 2 0.15882088356438828 1106 3 0.17137632118066648 1106 4 0.16958554012416016 1106 5 0.20598077499032996 1106 6 0.14608275139679705 1106 7 0.1725074283202801 1106 8 0.15406872574214248 1106 9 0.0808586724348146 1106 10-11 0.21796864944418104 1106 12-13 0.1327518522045068 1106 14-15 0.0644344911359056 1106 16-17 0.1224994204211427 1106 18-19 0.2372599562800204 1106 20-21 0.2279969054380402 1106 22-23 0.2283415800786628 1106 24-25 0.20021892354649395 1106 26-27 0.1820217032831266 1106 28-29 0.2210924593019712 1106 30-31 0.15096281053398997 1106 32-33 0.11367105048253734 1106 34-35 -0.05625448662371468 1106 36-37 0.08113636075743358 1106 38-39 0.13602113375635128 1106 40-41 0.06014077879555657 1106 42-43 0.1693455103849928 1106 44-45 0.058890907928848435 1106 46-47 0.02702665967822071 1106 48-49 -0.0022227031830155397 1106 50-51 0.09532131894278706 1106 52-53 -0.06253520602243867 1106 54-55 -0.07457991261548358 1106 56-57 -0.22664376251886154 1106 58-59 -0.06188221347748879 1106 60-61 0.047153246368630164 1106 62-63 -0.02527713095265227 1106 64-65 0.22631561289306035 1106 66-67 0.16551656939919468 1106 68-69 0.1829639221188657 1106 70-71 0.11418051116693562 1106 72-73 0.25820654658171094 1106 74-75 0.3027207689738205 1106 76-77 0.10106219060308064 1106 78-79 0.17762551942617222 1106 80-81 0.0658785268654043 1106 82-83 0.23638696950786198 1106 84-85 0.21474575938692908 1106 86-87 0.21770878606257327 1106 88-89 0.1369146332454818 1106 90-91 0.14722012324805434 1106 92-93 0.1857737384530509 1106 94-95 0.24520355901500324 1106 96-97 0.01699235865092419 1106 98-99 0.24565068114610966 1106 100-101 0.19560655693506845 1107 1 0.13730829841339443 1107 2 0.1077538044345232 1107 3 0.1565596145099306 1107 4 0.1613023964735163 1107 5 0.1547859955860389 1107 6 0.06784418122288827 1107 7 0.1985895578396324 1107 8 0.1046521943769605 1107 9 0.09179224831179766 1107 10-11 0.14634573532392636 1107 12-13 0.1018692348179897 1107 14-15 0.06074030462574598 1107 16-17 0.12107647450611836 1107 18-19 0.11035628832852495 1107 20-21 0.0808783709159755 1107 22-23 0.12467458417116006 1107 24-25 0.07444935565120403 1107 26-27 0.18202691553922534 1107 28-29 0.15284230923243314 1107 30-31 0.16310736724464192 1107 32-33 0.20106972639574394 1107 34-35 0.20720811934796757 1107 36-37 0.17453955569667556 1107 38-39 0.29318585755455473 1107 40-41 0.18027329171977868 1107 42-43 0.2576485127313717 1107 44-45 0.21236492005429852 1107 46-47 0.30463923789464076 1107 48-49 0.3010915098575282 1107 50-51 0.3026796346196008 1107 52-53 0.16931550106354365 1107 54-55 0.2289844887178205 1107 56-57 0.15768324400428213 1107 58-59 0.1660575641155404 1107 60-61 0.4011219015978682 1107 62-63 0.39060053044703125 1107 64-65 0.2205912526323459 1107 66-67 0.2451633174291814 1107 68-69 0.30311598100346515 1107 70-71 0.2537790632021881 1107 72-73 0.38228126253547856 1107 74-75 0.3420641810730274 1107 76-77 0.32582726987715205 1107 78-79 0.2596946634781574 1107 80-81 0.45165410622938396 1107 82-83 0.4132201416956107 1107 84-85 0.3980026028620145 1107 86-87 0.3441807054671955 1107 88-89 0.23610212939058073 1107 90-91 0.3954103844950154 1107 92-93 0.45453807758676135 1107 94-95 0.6938293911299738 1107 96-97 0.43723571888818924 1107 98-99 0.5662022594606881 1107 100-101 0.6043308311935576 1108 1 0.13100330649010772 1108 2 0.05019371985779486 1108 3 0.06994998667081376 1108 4 0.14503834540806793 1108 5 0.13082286731189186 1108 6 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-0.7386726473144734 2316 60-61 -0.7573672735996908 2316 62-63 -0.8636521885104571 2316 64-65 -0.6568260369490204 2316 66-67 -0.7424828910081942 2316 68-69 -0.8423061191117327 2316 70-71 -0.7973100983390964 2316 72-73 -1.16176757407451 2316 74-75 -0.9307856710940925 2316 76-77 -0.5635994674986833 2316 78-79 -0.6731447188763156 2316 80-81 -0.8382492058583964 2316 82-83 -1.2742952307940385 2316 84-85 -1.3148165300231085 2316 86-87 -0.9718321608018741 2316 88-89 -0.7215832343840063 2316 90-91 -0.516163016894545 2316 92-93 -0.3272382810476415 2316 94-95 -0.5475989086414685 2316 96-97 -0.5085440875891436 2316 98-99 -0.47358669965931455 2316 100-101 -0.6590007424868354 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 1.0 12 3.0 13 11.0 14 42.0 15 144.0 16 316.0 17 689.0 18 1196.0 19 1812.0 20 2530.0 21 3629.0 22 4654.0 23 5013.0 24 4939.0 25 5202.0 26 5969.0 27 7296.0 28 9095.0 29 11446.0 30 14323.0 31 18154.0 32 23423.0 33 31218.0 34 44782.0 35 67985.0 36 124600.0 37 258741.0 38 344941.0 39 102762.0 40 170.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.4145488233053 18.55293824214357 13.743834218477199 23.288678716073928 2 15.491858069606382 20.32633470675901 39.60360597294059 24.57820125069402 3 19.498448518010523 24.43561304084991 30.010629340999124 26.055309100140445 4 12.332119914738012 16.583894836243285 38.58917713415247 32.49480811486624 5 12.971401332863355 38.11298838630026 35.398498382775415 13.517111898060975 6 31.554142779653837 37.56179879936371 17.24129429104198 13.64276412994048 7 27.909588835945303 32.35654551332042 21.670078879649633 18.063786771084644 8 25.64501783421576 35.32791031937217 19.760183218486947 19.266888627925113 9 25.330065401256157 16.488841972228666 20.423784068100588 37.75730855841459 10-11 24.888090980982316 25.759894656675364 28.74326765203829 20.608746710304032 12-13 25.888971277141703 23.332825001872 28.446898234476564 22.331305486509738 14-15 22.52174715045211 25.316230871365352 26.422308384912235 25.739713593270302 16-17 22.18752682437726 28.37411856237775 26.91313741569155 22.525217197553435 18-19 22.05283420662852 28.269743198251096 28.030538240832225 21.646884354288158 20-21 23.125307053509953 26.591747132188704 27.861373444642705 22.421572369658637 22-23 22.580699658056357 26.591562955024983 27.877652757123215 22.95008462979544 24-25 22.971300884131473 26.684022989975215 27.87141831783075 22.473257808062563 26-27 22.62425051548463 26.41011756154311 28.506710888459903 22.458921034512358 28-29 22.334093246454803 27.426652856707058 27.888994229409736 22.350259667428407 30-31 22.667261540794012 26.576488741763587 27.916051509279992 22.84019820816241 32-33 22.618634518202224 26.848074032541735 27.94712013485699 22.58617131439905 34-35 23.143570459306392 26.6102388305576 27.94794198811783 22.29824872201818 36-37 22.944453677610706 26.502530394873098 28.237736579592838 22.315279347923358 38-39 22.65758123106313 27.04463393742592 28.04067443104925 22.2571104004617 40-41 22.74771113866856 27.05540934684582 27.649472278889515 22.547407235596108 42-43 22.52453231983607 26.98938713489169 27.486836650272217 22.999243895000028 44-45 22.856013135041447 26.654436272584984 27.493228842300972 22.996321750072596 46-47 23.2389510960783 26.9790683106167 27.335569991763204 22.446410601541796 48-49 22.925363890314955 27.032843761475768 27.408472473686807 22.63331987452247 50-51 22.211506497017254 27.247060177320897 27.98534929002232 22.55608403563953 52-53 22.84537470116502 27.46957773179458 26.97148899721118 22.71355856982922 54-55 22.539190005492742 27.010409727495166 27.31294428770827 23.137455979303823 56-57 23.000431016376798 26.88683811134468 27.18087894466736 22.931851927611167 58-59 23.50436404081506 26.7574875397914 27.072576948294476 22.665571471099074 60-61 22.83788670478848 27.087552941047555 27.778274948268905 22.296285405895063 62-63 22.477960635055148 27.138051258074707 27.660749931968816 22.72323817490133 64-65 22.41495188505743 27.659654127621025 27.206265078724414 22.719128908597135 66-67 22.73278080442997 27.017467121303717 26.996875131268226 23.252876942998085 68-69 22.602517062586866 26.99861015481889 27.162432904812956 23.23643987778129 70-71 22.991664581594502 26.980027139421015 26.97861172547179 23.049696553512693 72-73 22.80829998739825 26.59900683599279 27.653353252621255 22.939339923987706 74-75 22.33778899556747 27.697322402076185 27.086913721844674 22.87797488051167 76-77 22.699769698452908 26.91761195011168 26.89811576442398 23.48450258701143 78-79 22.666550998324333 27.034339800106842 27.141728724254282 23.157380477314547 80-81 22.996345597022046 26.896734690709106 26.974681284278578 23.13223842799027 82-83 22.692884242286706 26.73738849166904 27.551786850087417 23.01794041595684 84-85 22.612653252803888 27.1149937082567 27.21279424629664 23.059558792642772 86-87 22.57731356258778 27.298540936510925 27.005824200108485 23.118321300792815 88-89 22.539234361502203 27.098602301554397 26.591016595956845 23.771146740986556 90-91 22.834370999172666 26.764564609537516 26.74803622729174 23.65302816399808 92-93 23.027643491013492 27.008746345035917 26.932405308806796 23.0312048551438 94-95 22.68570687598965 27.001304007173864 27.116363463691435 23.196625653145052 96-97 22.0585871794544 27.0426706213028 27.596280109507383 23.302462089735418 98-99 22.582564291754256 27.20663034684034 26.61352624360096 23.597279117804444 100-101 22.345505284516467 26.87985235862754 26.822824874028157 23.951817482827835 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 30.0 1 19.5 2 27.0 3 58.5 4 51.0 5 40.5 6 74.5 7 158.0 8 267.5 9 352.5 10 362.0 11 347.5 12 385.5 13 422.5 14 470.5 15 543.0 16 598.5 17 638.0 18 694.5 19 780.5 20 890.5 21 1061.5 22 1337.5 23 1752.5 24 2201.5 25 2747.0 26 3758.5 27 5277.5 28 7052.5 29 9088.0 30 11514.5 31 14155.5 32 16538.5 33 18877.0 34 21488.0 35 23563.0 36 25645.5 37 28268.5 38 30008.5 39 30659.0 40 31679.0 41 33161.0 42 34307.0 43 35562.0 44 37011.5 45 38723.0 46 40282.0 47 45690.5 48 55566.5 49 54820.0 50 46545.0 51 42969.5 52 40941.5 53 39269.0 54 37949.5 55 36172.0 56 33333.5 57 30137.0 58 27232.0 59 23233.5 60 18524.5 61 14496.0 62 10811.5 63 7702.0 64 5336.5 65 3555.5 66 2292.0 67 1597.5 68 1192.0 69 827.0 70 587.5 71 406.0 72 282.5 73 224.0 74 171.0 75 115.5 76 69.0 77 43.0 78 29.0 79 15.0 80 7.5 81 3.5 82 2.5 83 3.5 84 3.5 85 3.5 86 3.5 87 3.5 88 1.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04903724456344068 2 0.001278438405750781 3 3.6526811592879464E-4 4 0.009862239130077456 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.00205463315209947 24-25 0.0 26-27 0.0 28-29 0.020455014492012497 30-31 0.016117455615358062 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.035431007245093085 50-51 0.011140677535828236 52-53 0.0 54-55 4.1092663041989396E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 9.131702898219866E-5 80-81 0.009633946557621958 82-83 0.00406360778970784 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1095086.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.877908377982195 #Duplication Level Percentage of deduplicated Percentage of total 1 72.88080440251396 44.36830932929887 2 15.43629283843094 18.79458442227403 3 5.489641133631347 10.025936098836343 4 2.5314779526534004 6.164443314500626 5 1.3201374340953929 4.018360287960192 6 0.7463582666860346 2.726203810587721 7 0.4554610874048385 1.9409262844137527 8 0.2970612610813916 1.4467574587808645 9 0.18826509576470518 1.031506672566219 >10 0.6380721408117361 5.782907659007974 >50 0.009349222437507895 0.39117351670737294 >100 0.0042173756222783545 0.5807020180443822 >500 0.0010543432665901236 0.4932251979380224 >1k 0.0016568251332130513 1.6534368412750362 >5k 1.5062046665573192E-4 0.581527087808689 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 6342 0.5791325978051038 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 3091 0.28226093658397605 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 2192 0.20016692752897947 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1772 0.16181377535645602 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 1744 0.15925689854495445 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 1545 0.14108480977749693 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 1540 0.14062822463258592 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1448 0.13222705796622367 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 1353 0.1235519402129148 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 1247 0.11387233514080172 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.131702898219866E-5 0.0 20-21 0.0 0.0 0.0 3.6526811592879464E-4 0.0 22-23 0.0 0.0 0.0 9.58828804313086E-4 0.0 24-25 0.0 0.0 0.0 0.0016437065216795758 0.0 26-27 0.0 0.0 0.0 0.00273951086946596 0.0 28-29 0.0 0.0 0.0 0.004337558876654437 0.0 30-31 0.0 0.0 0.0 0.0074879963765402905 0.0 32-33 0.0 0.0 0.0 0.015386919383500475 0.0 34-35 0.0 0.0 0.0 0.02570574365848892 0.0 36-37 0.0 0.0 0.0 0.041960174817320284 0.0 38-39 0.0 0.0 0.0 0.06880738133808668 0.0 40-41 0.0 0.0 0.0 0.10063136593838293 0.0 42-43 0.0 0.0 0.0 0.13117691213292837 0.0 44-45 0.0 0.0 0.0 0.16802333332724553 0.0 46-47 0.0 0.0 0.0 0.2118098487242098 0.0 48-49 0.0 0.0 0.0 0.25527675451973636 0.0 50-51 0.0 0.0 0.0 0.30180278078616657 0.0 52-53 0.0 0.0 0.0 0.35161622009595594 0.0 54-55 0.0 0.0 0.0 0.4079588269779725 0.0 56-57 0.0 0.0 0.0 0.47877518295366756 0.0 58-59 0.0 0.0 0.0 0.5503677336757113 0.0 60-61 0.0 0.0 0.0 0.6270740380207582 0.0 62-63 0.0 0.0 0.0 0.7098985833076124 0.0 64-65 0.0 0.0 0.0 0.7959192246088436 0.0 66-67 0.0 0.0 0.0 0.8922586901850631 0.0 68-69 0.0 0.0 0.0 1.014075606847316 0.0 70-71 0.0 0.0 0.0 1.1423760325673054 0.0 72-73 0.0 0.0 0.0 1.2739182128161624 0.0 74-75 0.0 0.0 0.0 1.4101175615431116 0.0 76-77 0.0 0.0 0.0 1.5470931050164096 0.0 78-79 0.0 0.0 0.0 1.7281747734881097 0.0 80-81 0.0 0.0 0.0 1.9231366303651036 0.0 82-83 0.0 0.0 0.0 2.1251755569882187 0.0 84-85 0.0 0.0 0.0 2.3336066756400866 0.0 86-87 0.0 0.0 0.0 2.548384327806218 0.0 88-89 0.0 0.0 0.0 2.806263617651947 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAAG 410 0.0 25.5047 1 GTACATG 2990 0.0 24.958002 1 TACATGG 2900 0.0 23.750547 2 ACATGGG 2865 0.0 23.211704 3 TCTATAC 440 0.0 21.591406 3 GTATTAC 310 2.564775E-10 19.932556 1 GTACGGG 120 0.0047451337 19.804785 1 AGAGTAC 3485 0.0 19.761923 10-11 GGGTATA 220 1.3361205E-6 19.444696 1 GTGTACG 220 1.3361205E-6 19.444696 1 TATACTG 550 0.0 18.135126 5 CATGGGG 1750 0.0 17.914698 4 GGATCGT 160 0.001191511 17.811285 6 CTATACT 375 1.70985E-10 17.733742 4 GTACCTG 860 0.0 17.686132 1 ATTATAC 565 0.0 17.655275 3 TATACTC 355 1.5843398E-9 17.393179 5 AGTACTT 2395 0.0 16.856886 12-13 GTACTAA 285 8.522238E-7 16.677713 1 GAGTACT 2165 0.0 16.563536 12-13 >>END_MODULE