FastQCFastQC Report
Fri 10 Feb 2017
SRR2031593_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031593_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537667
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT129090.8395185693651487No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT72040.46850195783612447No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT51460.33466283662197344No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC45820.2979838937819437No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT34110.22182956387826494No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT29800.19380008805547624No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC29060.18898760264738723No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC23830.1549750368577852No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA22810.14834161102501386No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC21140.13748100206351568No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG19660.1278560312473377No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA18390.11959676574967143No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG40250.024.6100031
ACCGCTC1801.9506297E-723.7516218
ACATGGG39150.023.426343
TACATGG41000.023.3178042
TGCACCG1452.3273135E-522.940825
GTCCGTA1150.003668580720.705531
CCGTATA1150.003695043720.6798612
TACACCG1656.303756E-520.1601165
CATGGGG25550.019.528884
GAGTACA33000.019.337711
TCCGTAT1250.00594280919.0490881
GTCCTAC4400.018.3996871
AAGACCG2603.267196E-718.2770275
AGCGTGC2101.9553467E-518.0964728
GAGTACT23800.017.76323912-13
GTAGGAC4600.017.561843
AGTACAT31500.017.5155142
GTATTAG6800.017.5083521
TAGGTCG2202.9075458E-517.28015
GTATATA11700.017.0953351
Produced by FastQC (version 0.11.5)