FastQCFastQC Report
Fri 10 Feb 2017
SRR2031593_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031593_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1537667
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT143140.930890758532244No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT62860.40880112534118246No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT45200.29395181141300425No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC40830.2655321340706408No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT36780.23919353149934286No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC32530.21155425719612894No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC28360.18443525158568144No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT27470.17864726237865547No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA26030.16928242590886064No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG23350.15185342470118693No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC20350.1323433487224477No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA19980.12993710601840322No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC16820.10938649265413121No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15460.10054192487710278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG804.4800848E-429.703611
GTACATG40250.026.56721
ACATGGG37350.026.3267353
TACATGG40050.025.9751972
TATACTG9650.022.640045
ACCGTCC1954.227768E-721.9207068
CTATACT10350.021.5712224
CATGGGG23050.020.1963274
TATACAG11150.020.0202565
CCGTGCA2251.6701088E-618.9979469
TACACCG2302.0609077E-618.5849465
GAGTACT25450.018.19547812-13
GTATAGA7850.018.1627161
TCTATAC7100.018.0643633
GTAGGAC4500.017.9454213
CATGGGA17650.017.7630064
GAGTACA32350.017.335521
TGCACCG1952.2778231E-417.0494385
AGTACAT30650.016.8932532
TAGGACT9000.016.8898074
Produced by FastQC (version 0.11.5)