FastQCFastQC Report
Fri 10 Feb 2017
SRR2031592_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031592_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390029
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14490.10424242947449297No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACA1309.926724E-625.608772
GTACATG45650.025.5532071
ACATGGG42850.024.6210863
TACATGG45950.024.1159522
ACCGTGT2806.548362E-1122.0555638
GCGTTAC1353.7363707E-421.161071
TACACCG1805.141841E-621.124445
CATGGGG23500.021.0345464
GTATTAT4600.020.7010461
GCTACAC8850.020.4054133
TAAGGCG1456.120847E-419.667585
ATGGGCG1707.9168836E-519.571175
ACACCGT3054.5620254E-918.6895376
CCGTGTA1801.2327006E-418.473899
CATGGGA19000.018.0113624
GTATCAA38850.017.6478421
GGTATCA31300.017.3412591
TACACTG14550.017.3133285
GTATAGG8350.017.1062531
GAGTACA32650.016.9158921
Produced by FastQC (version 0.11.5)