##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031592_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1390029 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.05948293165107 33.0 31.0 34.0 30.0 34.0 2 32.29447802887566 34.0 31.0 34.0 30.0 34.0 3 32.446743197444086 34.0 31.0 34.0 31.0 34.0 4 35.532425582487846 37.0 35.0 37.0 33.0 37.0 5 35.254278148153745 37.0 35.0 37.0 32.0 37.0 6 35.47262179422156 37.0 35.0 37.0 33.0 37.0 7 35.62065899344546 37.0 35.0 37.0 33.0 37.0 8 35.69843434921142 37.0 35.0 37.0 33.0 37.0 9 37.639817586539564 39.0 38.0 39.0 35.0 39.0 10-11 37.59558145909186 39.0 38.0 39.0 35.0 39.0 12-13 37.62484991320325 39.0 38.0 39.0 35.0 39.0 14-15 39.08202850444127 41.0 39.0 41.0 36.0 41.0 16-17 39.0517438844801 41.0 39.0 41.0 36.0 41.0 18-19 38.9778777996718 41.0 39.0 41.0 36.0 41.0 20-21 38.9819198016732 41.0 39.0 41.0 35.5 41.0 22-23 38.92610693733728 41.0 39.0 41.0 35.0 41.0 24-25 38.866962128128264 41.0 39.0 41.0 35.0 41.0 26-27 38.79346510036841 41.0 39.0 41.0 35.0 41.0 28-29 38.70161090164306 40.0 38.5 41.0 34.5 41.0 30-31 38.6003410720208 40.0 38.0 41.0 34.0 41.0 32-33 38.487070773343575 40.0 38.0 41.0 34.0 41.0 34-35 38.38401572916824 40.0 38.0 41.0 34.0 41.0 36-37 38.28397716882166 40.0 38.0 41.0 34.0 41.0 38-39 38.137551087063656 40.0 38.0 41.0 33.0 41.0 40-41 38.02584298600964 40.0 38.0 41.0 33.0 41.0 42-43 37.886673947090316 40.0 38.0 41.0 33.0 41.0 44-45 37.72609923965615 40.0 38.0 41.0 32.5 41.0 46-47 37.58362559342287 40.0 37.5 41.0 32.0 41.0 48-49 37.756218755148275 40.0 38.0 41.0 32.5 41.0 50-51 37.53569637755759 39.5 37.5 40.5 32.5 41.0 52-53 37.68304438252727 40.0 37.5 40.5 33.0 41.0 54-55 37.981992462027776 40.0 38.0 41.0 33.0 41.0 56-57 37.91138817967107 40.0 37.5 41.0 33.0 41.0 58-59 37.756610473594435 40.0 37.0 41.0 33.0 41.0 60-61 37.51623167574202 40.0 37.0 41.0 32.0 41.0 62-63 37.2359216965977 40.0 36.0 41.0 31.0 41.0 64-65 37.00718330336993 39.0 36.0 41.0 31.0 41.0 66-67 36.82683850480817 39.0 35.0 41.0 31.0 41.0 68-69 36.616363039907796 39.0 35.0 41.0 31.0 41.0 70-71 36.33621744582307 38.0 35.0 40.5 31.0 41.0 72-73 35.98129535426959 37.0 35.0 40.0 30.5 41.0 74-75 35.5505417512872 37.0 35.0 39.0 30.0 41.0 76-77 35.14020103177704 36.0 35.0 39.0 29.5 41.0 78-79 34.73706807555814 36.0 34.0 38.5 29.0 39.5 80-81 34.346642408179974 35.5 34.0 37.0 29.0 39.0 82-83 33.96918409615914 35.0 34.0 37.0 29.0 39.0 84-85 33.64312363267241 35.0 34.0 36.5 29.0 38.0 86-87 33.35948890274951 35.0 34.0 36.0 28.5 37.0 88-89 33.07577144073973 35.0 34.0 36.0 27.5 37.0 90-91 32.85011463789604 35.0 34.0 35.0 27.0 36.0 92-93 32.64955047700444 35.0 33.5 35.0 26.5 36.0 94-95 32.46762225824065 35.0 33.0 35.0 26.0 36.0 96-97 32.34552696382593 35.0 33.0 35.0 25.5 36.0 98-99 32.22673411849681 35.0 33.0 35.0 25.0 35.0 100-101 31.75212819300892 34.5 32.0 35.0 24.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.014691097005552933 1101 2 0.20965176585006873 1101 3 0.2769630112612944 1101 4 0.2967713166303625 1101 5 0.14464390660555893 1101 6 0.10767311265917812 1101 7 0.2157897305754588 1101 8 0.31656642553241454 1101 9 0.27979695051607223 1101 10-11 0.331513837606245 1101 12-13 0.3091714755591468 1101 14-15 0.4401146132225833 1101 16-17 0.3724309582750962 1101 18-19 0.3445818158133491 1101 20-21 0.08390699948446212 1101 22-23 0.26602976197758466 1101 24-25 0.3732659442840571 1101 26-27 0.40571207416788013 1101 28-29 0.28025621456371397 1101 30-31 0.24500448679905418 1101 32-33 0.4603117529158638 1101 34-35 0.4448458742686583 1101 36-37 0.31914846231477867 1101 38-39 0.3877964940758005 1101 40-41 0.21910849898591067 1101 42-43 0.31737597745729573 1101 44-45 0.2279121949728946 1101 46-47 0.3757329968701768 1101 48-49 0.39008495957195777 1101 50-51 0.468448147833584 1101 52-53 0.3613959717815973 1101 54-55 0.22018355781606402 1101 56-57 0.4081943934421588 1101 58-59 0.4455417055597124 1101 60-61 0.3662512290677924 1101 62-63 -0.010507875977964432 1101 64-65 0.16262428730566825 1101 66-67 0.2796026364840358 1101 68-69 0.2449984138236161 1101 70-71 0.37027567654957494 1101 72-73 0.41531048262151415 1101 74-75 0.2775934328073646 1101 76-77 0.14419486860907682 1101 78-79 0.2907149029807172 1101 80-81 0.16801829180477768 1101 82-83 0.1663380044541185 1101 84-85 0.25033485116426135 1101 86-87 0.16792531491994822 1101 88-89 0.247477105778934 1101 90-91 -0.054263054096132635 1101 92-93 0.014111545277032178 1101 94-95 -0.14206314953268873 1101 96-97 0.14490349159970606 1101 98-99 -0.009350754423266494 1101 100-101 0.10870182649735227 1102 1 0.12908071999015647 1102 2 0.14511869113394482 1102 3 0.086115635821848 1102 4 0.005495606443268741 1102 5 0.050959823686412165 1102 6 0.15697605847078933 1102 7 0.04937764022107416 1102 8 0.15169795662708907 1102 9 0.22204045477921852 1102 10-11 0.1978208647206401 1102 12-13 0.30312831881287394 1102 14-15 0.2513139505159998 1102 16-17 0.3016446003327218 1102 18-19 0.33502712231514664 1102 20-21 0.21031828166007926 1102 22-23 0.08312476339190056 1102 24-25 0.26887551312067615 1102 26-27 0.27670855859022936 1102 28-29 0.13270701668114526 1102 30-31 0.2563351531424587 1102 32-33 0.23487432615706894 1102 34-35 0.21155294987378426 1102 36-37 0.1928422433791468 1102 38-39 -0.02585768622888196 1102 40-41 0.10266206103383269 1102 42-43 -0.11718174671128878 1102 44-45 -0.22450223909992673 1102 46-47 -0.6657750647130385 1102 48-49 0.06412423706014181 1102 50-51 -0.018285597670924858 1102 52-53 0.06557627646498787 1102 54-55 0.10111660020503876 1102 56-57 0.11724801610349544 1102 58-59 -0.032976902756438164 1102 60-61 -0.3279319042413036 1102 62-63 -0.5720345256728692 1102 64-65 -0.1836661721439654 1102 66-67 0.1132895480288667 1102 68-69 0.17447471398121905 1102 70-71 0.21620649644693657 1102 72-73 0.05343227516202376 1102 74-75 -0.04390816334570502 1102 76-77 0.09965411619464248 1102 78-79 -0.0017750958070124057 1102 80-81 0.03426066461764776 1102 82-83 -0.03719575721395785 1102 84-85 -0.021320298954954353 1102 86-87 0.061478068789107 1102 88-89 -0.23077278309649074 1102 90-91 -0.1734310340533014 1102 92-93 -0.24658268028566255 1102 94-95 -0.1646598279179443 1102 96-97 -0.09965544483703326 1102 98-99 -0.20761519051653465 1102 100-101 -0.10101854364852159 1103 1 -0.025893157821975876 1103 2 -0.11325944847887115 1103 3 -0.10344158167011841 1103 4 0.0629507113128085 1103 5 -0.05454030578215452 1103 6 0.039901438879908824 1103 7 -0.0015217855477445141 1103 8 0.05632074614165816 1103 9 0.34555194701871983 1103 10-11 0.262042606301371 1103 12-13 0.2788659088696974 1103 14-15 0.269335213824597 1103 16-17 0.2519309543454824 1103 18-19 0.24178157374920062 1103 20-21 0.062001373064887844 1103 22-23 0.11614686443831346 1103 24-25 0.27549364176674374 1103 26-27 0.04315106692952497 1103 28-29 0.13666266227235724 1103 30-31 0.3153842445777286 1103 32-33 0.22536295577033627 1103 34-35 0.18742990903786705 1103 36-37 0.12121033013831806 1103 38-39 0.2133987072337149 1103 40-41 0.14655723718724545 1103 42-43 0.1772145489644643 1103 44-45 0.2236265592794382 1103 46-47 0.19559836833647637 1103 48-49 -0.07509788531050532 1103 50-51 0.1174987533869114 1103 52-53 0.07028436291953 1103 54-55 0.01595293670145992 1103 56-57 0.1689996496922106 1103 58-59 0.3291870501474605 1103 60-61 0.33176376846216016 1103 62-63 0.3021136472971335 1103 64-65 0.31190752593533233 1103 66-67 0.11961827634288369 1103 68-69 0.06241052803388669 1103 70-71 0.24595608900173715 1103 72-73 0.20143541287088595 1103 74-75 0.10035257112187423 1103 76-77 -0.3641449485853343 1103 78-79 -0.17602760521253202 1103 80-81 0.09775052799022177 1103 82-83 -0.2299379015180989 1103 84-85 -0.15897204373431606 1103 86-87 -0.03807716859532917 1103 88-89 -0.0010795474484623924 1103 90-91 0.05597280126267634 1103 92-93 -0.210591179516463 1103 94-95 -0.22385057319995383 1103 96-97 -0.20077039769524418 1103 98-99 -0.023454527637454703 1103 100-101 0.07189510843135594 1104 1 0.01367511527039511 1104 2 0.04401249644367766 1104 3 0.11948584231151926 1104 4 -0.07024923131484684 1104 5 -0.23501362764101685 1104 6 -0.039712732089682845 1104 7 -0.039324424675683645 1104 8 0.1533130008748813 1104 9 0.2166348500594495 1104 10-11 0.34091452253775145 1104 12-13 0.30793289108427047 1104 14-15 0.31050274107646914 1104 16-17 0.19245949708788146 1104 18-19 0.29133980668093074 1104 20-21 0.2089355382972542 1104 22-23 0.2201564743063571 1104 24-25 0.17336297624752461 1104 26-27 0.22419380932769428 1104 28-29 0.2996797305454493 1104 30-31 0.16284695255248494 1104 32-33 0.09178435565559084 1104 34-35 0.03079336285313161 1104 36-37 0.23900006048829425 1104 38-39 0.3054734347150685 1104 40-41 0.08723064510463274 1104 42-43 0.14558374001438068 1104 44-45 0.2152580853838515 1104 46-47 0.24638938956424283 1104 48-49 0.0538292518094039 1104 50-51 0.18425865011668918 1104 52-53 0.25112199917885647 1104 54-55 0.25798606923158474 1104 56-57 0.21710421079375664 1104 58-59 0.12580997039989938 1104 60-61 0.20851721080294539 1104 62-63 0.3073460053005803 1104 64-65 0.372270406027134 1104 66-67 0.2947681615981921 1104 68-69 0.20785229510215686 1104 70-71 0.25378024275961764 1104 72-73 0.38258787988178966 1104 74-75 0.33726809034160965 1104 76-77 0.3379505763716324 1104 78-79 0.3110973230720404 1104 80-81 0.17931966166779745 1104 82-83 0.2626051277417929 1104 84-85 0.22040905207749972 1104 86-87 0.18721184460031282 1104 88-89 0.11933783637253725 1104 90-91 -0.013829264141797637 1104 92-93 0.13306702472909393 1104 94-95 0.09035123402895806 1104 96-97 -0.031392170500751604 1104 98-99 0.07971888027993401 1104 100-101 0.021241780835254076 1105 1 -0.0905255650017196 1105 2 0.03064514950489894 1105 3 0.12348244095097982 1105 4 0.11231879589603722 1105 5 -0.05062587253897988 1105 6 -0.03768892256587009 1105 7 0.08142387464404521 1105 8 0.14713953148712022 1105 9 0.19141376162407653 1105 10-11 0.21718152934047197 1105 12-13 0.1373291495876643 1105 14-15 0.16659117645061627 1105 16-17 0.2392877283803969 1105 18-19 0.25773436450405285 1105 20-21 0.2860018648278668 1105 22-23 0.2273418484560139 1105 24-25 0.14743610549922437 1105 26-27 0.2305373467426648 1105 28-29 0.13407598904884566 1105 30-31 0.14755783690622337 1105 32-33 0.30190340327463616 1105 34-35 0.15240900911163635 1105 36-37 0.1959558428012187 1105 38-39 0.1959411217899003 1105 40-41 0.22281737979850647 1105 42-43 0.17634904613682778 1105 44-45 0.389144139805623 1105 46-47 0.5825543555506343 1105 48-49 0.46416938786381934 1105 50-51 0.2943096705248536 1105 52-53 0.41554376788634073 1105 54-55 0.3858177018846405 1105 56-57 0.5233542107937623 1105 58-59 0.46479806563799286 1105 60-61 0.49078761896621614 1105 62-63 0.4010194746883329 1105 64-65 0.4889200658910795 1105 66-67 0.38394333166622374 1105 68-69 0.2240087576871943 1105 70-71 0.3531850046643825 1105 72-73 0.3595691723987997 1105 74-75 0.43237863455929926 1105 76-77 0.48616486208592136 1105 78-79 0.5588949421196574 1105 80-81 0.3246597977222123 1105 82-83 0.4069418624356729 1105 84-85 0.20995837180539212 1105 86-87 0.2516081031037132 1105 88-89 0.3117357955562099 1105 90-91 0.41545645014391397 1105 92-93 0.314146956701876 1105 94-95 0.1638971523963022 1105 96-97 0.17404150296863463 1105 98-99 -0.018622956454763084 1105 100-101 -0.0059435933144058595 1106 1 0.28028272681894606 1106 2 0.18712273266415025 1106 3 0.2082325614716254 1106 4 0.25369433823895804 1106 5 0.21683676605977098 1106 6 0.021501178671471166 1106 7 0.19671232073114453 1106 8 0.30750591426070173 1106 9 0.24883301278911318 1106 10-11 0.34146242280021966 1106 12-13 0.24921569948321576 1106 14-15 0.25647386968539365 1106 16-17 0.3169640902637312 1106 18-19 0.43593298253394863 1106 20-21 0.10888632569882617 1106 22-23 0.28972012260032187 1106 24-25 0.22617465603754994 1106 26-27 0.15817674896024414 1106 28-29 0.19549009799951733 1106 30-31 0.2839952465157367 1106 32-33 0.3569877677290805 1106 34-35 0.3451568799134961 1106 36-37 0.2934554410657242 1106 38-39 0.3687838021691334 1106 40-41 0.3317680466794357 1106 42-43 0.42623334631358745 1106 44-45 0.4561471929954024 1106 46-47 0.5611498882519115 1106 48-49 0.4710470995784277 1106 50-51 0.15502636665212322 1106 52-53 0.22841858710536656 1106 54-55 0.24047622671189828 1106 56-57 0.2654014548882415 1106 58-59 0.24465183391696144 1106 60-61 0.22126327379507416 1106 62-63 0.3638026982139664 1106 64-65 0.5814895661321131 1106 66-67 0.36096566815987075 1106 68-69 0.35605439483968837 1106 70-71 0.28760570207720804 1106 72-73 0.34317515022299716 1106 74-75 0.3279997176381997 1106 76-77 0.4157261669228163 1106 78-79 0.3890861682164015 1106 80-81 0.36350601337382926 1106 82-83 0.3415618206551869 1106 84-85 0.30360130929535245 1106 86-87 0.40814032229059904 1106 88-89 0.4036882300733282 1106 90-91 0.38447782247237683 1106 92-93 0.4834640063563853 1106 94-95 0.3452035962336808 1106 96-97 0.4813145224126316 1106 98-99 0.4397090377602453 1106 100-101 0.5469537230924715 1107 1 0.09994504693326434 1107 2 0.05245972499341178 1107 3 0.03847786964637123 1107 4 0.3011877466654127 1107 5 0.16493701776520453 1107 6 0.05754049494144908 1107 7 0.08095651685810878 1107 8 0.08452598492954877 1107 9 0.1588121469463175 1107 10-11 0.164375987989537 1107 12-13 0.07967833527560941 1107 14-15 0.08776545937563895 1107 16-17 0.04269962616913148 1107 18-19 0.28965415747819634 1107 20-21 0.09920224292898183 1107 22-23 0.14119956466323202 1107 24-25 0.06042542879119139 1107 26-27 0.016074978651914762 1107 28-29 0.14335000389396413 1107 30-31 0.1539840064628848 1107 32-33 0.20831624631617984 1107 34-35 0.21802381084098243 1107 36-37 0.1368037810653604 1107 38-39 0.3066209290203119 1107 40-41 0.13978854183963563 1107 42-43 0.05372155322621808 1107 44-45 0.10051909525477498 1107 46-47 0.32304371758247186 1107 48-49 0.21390518195367036 1107 50-51 0.08202820212883921 1107 52-53 0.07720400373466418 1107 54-55 0.13371784599696213 1107 56-57 0.26734813638221766 1107 58-59 0.29949163327005834 1107 60-61 0.24083023282269522 1107 62-63 0.20405823005381052 1107 64-65 0.2661295556095169 1107 66-67 0.1038503559793611 1107 68-69 0.08173612198142877 1107 70-71 0.09386186766470672 1107 72-73 0.24498252680661636 1107 74-75 0.34946911539856274 1107 76-77 0.21794488161081205 1107 78-79 0.13257321525123444 1107 80-81 0.2675315067703039 1107 82-83 0.21074673746085182 1107 84-85 0.0065033952817472596 1107 86-87 0.024420462671685073 1107 88-89 0.1498142980278132 1107 90-91 0.12484575484074156 1107 92-93 0.2678971310312974 1107 94-95 0.2547997913562199 1107 96-97 0.13713573382726452 1107 98-99 0.22148520526094728 1107 100-101 0.21143635182993847 1108 1 0.09474391585906261 1108 2 0.0472317753252085 1108 3 0.037026313246030895 1108 4 -0.00297366104258856 1108 5 -0.001132097103855756 1108 6 0.07708454532017583 1108 7 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-1.150485318341559 2316 76-77 -0.877073643219326 2316 78-79 -1.2175728419799867 2316 80-81 -1.1427381065417492 2316 82-83 -1.4187807710741396 2316 84-85 -1.916378264033014 2316 86-87 -1.4011389806598231 2316 88-89 -1.1233020990418865 2316 90-91 -1.3351478863161788 2316 92-93 -1.467880678894879 2316 94-95 -1.3432722858849289 2316 96-97 -1.1102982523091498 2316 98-99 -0.7145424221958478 2316 100-101 -1.4208200239727837 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 4.0 7 44.0 8 222.0 9 315.0 10 354.0 11 468.0 12 680.0 13 924.0 14 1173.0 15 1446.0 16 1712.0 17 2259.0 18 2777.0 19 3489.0 20 4083.0 21 4633.0 22 5268.0 23 5919.0 24 6805.0 25 7899.0 26 9257.0 27 10957.0 28 13128.0 29 15988.0 30 19490.0 31 23726.0 32 30594.0 33 39440.0 34 55657.0 35 82783.0 36 141565.0 37 265534.0 38 443326.0 39 188014.0 40 95.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.87161808122748 20.139848010147112 13.975044176793903 23.013489731831502 2 14.140217427402668 20.245526335168574 43.18095510379711 22.43330113363165 3 18.39857169011384 25.764718093318745 30.65900038489982 25.177709831667595 4 11.808126062549658 16.804254380336182 40.518776577259885 30.86884297985428 5 12.349363053879909 39.076292912284245 36.453582078250804 12.120761955585042 6 30.072461417700826 39.011676141503386 18.284596849121243 12.631265591674543 7 26.70894077132703 33.070093185138525 23.035807339059414 17.185158704475036 8 24.0217379552916 38.46616237877811 20.7632069250091 16.748892740921196 9 24.991837584876507 17.203127736571293 21.011822348368273 36.79321233018393 10-11 23.8711049358354 27.255059911587832 29.743035244124627 19.13079990845214 12-13 24.227570024581535 24.43241014729821 29.812454524519733 21.527565303600525 14-15 21.20882835892978 26.658470902514374 26.88037644290861 25.252324295647234 16-17 21.24345150151058 30.052171984666646 27.604171166734346 21.100205347088423 18-19 21.229292915844596 29.489672950683705 28.715526575343254 20.565507558128445 20-21 22.012501924424434 28.459179900232083 28.645327497471268 20.88299067787221 22-23 21.754006571085927 28.232396590286967 28.439478600806172 21.57411823782094 24-25 21.58372235399405 28.355991133997925 28.403759921555594 21.65652659045243 26-27 21.66080707668689 28.483938104888455 28.45185244336629 21.403402375058363 28-29 21.55296759995655 28.497570913988124 28.461528500484523 21.487932985570804 30-31 21.827170512269888 28.344084907581063 28.34117129930383 21.487573280845222 32-33 21.664512035360413 28.495412685634612 28.356890395811885 21.483184883193086 34-35 21.762207838829262 28.43023418935864 28.37617056910323 21.431387402708864 36-37 21.982383101359755 28.15714636169461 28.30750293698908 21.55296759995655 38-39 21.737244330873672 28.312538803147273 28.45919041976822 21.491026446210835 40-41 21.623433755698624 28.370882909637135 28.489585469080144 21.5160978655841 42-43 21.796343817287266 28.27987761406417 28.2463171631671 21.67746140548147 44-45 21.754684868841682 28.326018244198508 28.136408252212984 21.782888634746826 46-47 21.935852470775675 28.19063307656539 28.09056927507813 21.782945177580807 48-49 21.82978446123589 28.063383970476686 28.383176665033442 21.72365490325398 50-51 21.320564697548757 28.33414799014925 28.499322958996725 21.845964353305263 52-53 21.63566222697057 28.221665395388047 28.480264704326032 21.66240767331535 54-55 21.565378294654572 28.16017740690008 28.456779567966677 21.81766473047867 56-57 21.81422382593477 28.086096316382715 28.178897975793067 21.92078188188945 58-59 21.56180139777153 27.926626895958517 28.433283970993234 22.07828773527672 60-61 21.775887781788317 27.925807450954963 28.544318844965094 21.753985922291626 62-63 21.386943697218395 28.246839414110674 28.41292793215107 21.95328895651986 64-65 21.57520282740904 28.16624715623798 28.304094873231968 21.954455143121013 66-67 21.624691093074112 28.153803331567723 28.151534803363614 22.06997077199455 68-69 21.523082463089665 27.935974072740365 28.32517104789341 22.215772416276558 70-71 21.67355999717738 27.798819397140807 28.297529777891704 22.230090827790107 72-73 21.604172778666705 27.812368948220033 28.301424943439628 22.282033329673634 74-75 21.473210313653716 27.897648740835574 28.27746392674293 22.351677018767784 76-77 21.47913700185649 27.641498891512413 28.38377102070979 22.495593085921307 78-79 21.579809400172827 27.599621494692535 28.308832667010787 22.511736438123854 80-81 21.71535343207494 27.602803839258073 28.147154758390275 22.534687970276714 82-83 21.719825025652103 27.50398732696081 28.18534319814225 22.590844449244837 84-85 21.704144636687168 27.44116897276324 28.419105627240505 22.435580763309083 86-87 21.485257505878423 27.451982680791197 28.43954331890066 22.62321649442972 88-89 21.548191274479095 27.356527938301344 28.2436621696065 22.851618617613063 90-91 21.81837955880531 27.237124138944345 28.24752306952115 22.69697323272919 92-93 21.892934041639403 27.249762921157235 28.22056114904693 22.636741888156433 94-95 21.809388106170516 26.970166736061984 28.309004150752564 22.91144100701493 96-97 21.62011724305287 26.91952915558647 28.49934364916662 22.961009952194043 98-99 21.83840515458462 26.916346359113817 28.23026432078059 23.014984165520975 100-101 22.034698708795688 26.848216814918985 28.033959396874085 23.083125079411243 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 13.0 1 9.0 2 10.5 3 18.0 4 20.0 5 21.0 6 34.0 7 56.0 8 89.5 9 124.5 10 131.5 11 136.0 12 150.0 13 164.0 14 182.0 15 208.0 16 247.0 17 286.0 18 322.0 19 353.0 20 380.0 21 419.5 22 470.5 23 560.0 24 660.0 25 785.5 26 975.5 27 1168.5 28 1495.0 29 2225.5 30 3817.0 31 7101.0 32 13666.0 33 23303.0 34 36022.5 35 52400.0 36 69797.5 37 83012.0 38 87294.5 39 85987.0 40 82892.0 41 76887.0 42 69793.5 43 62684.0 44 55127.0 45 48384.5 46 43058.5 47 40171.0 48 40086.5 49 38427.0 50 35108.0 51 33186.0 52 32300.0 53 31701.5 54 30472.5 55 28656.0 56 26242.0 57 23472.0 58 21237.5 59 19212.5 60 16732.0 61 14003.5 62 11491.5 63 8899.0 64 6531.0 65 4915.0 66 3525.5 67 2501.5 68 1888.5 69 1378.0 70 1039.0 71 845.0 72 694.0 73 611.5 74 484.5 75 348.5 76 254.5 77 173.0 78 109.5 79 79.0 80 60.0 81 55.0 82 48.5 83 29.0 84 21.5 85 19.5 86 16.0 87 14.0 88 11.5 89 10.5 90 10.0 91 6.5 92 2.5 93 2.0 94 1.0 95 2.0 96 1.5 97 1.5 98 2.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4592709936267517 2 0.354165272810855 3 0.19100320928556166 4 0.21834076843001118 5 0.2398511110199859 6 0.19208232346231627 7 0.18531987462132085 8 0.18661481163342636 9 0.18503211084085297 10-11 0.19974403411727382 12-13 0.18744213250227154 14-15 0.1961829573339837 16-17 0.08352343728080493 18-19 0.03586256114081073 20-21 2.1582283535091716E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.13787482131667755 46-47 0.20963591407085752 48-49 0.08348746680824644 50-51 0.06445908682480725 52-53 0.07248050220534967 54-55 0.08140117939985425 56-57 0.11395445706528425 58-59 0.09485413613672808 60-61 0.14524876819116725 62-63 0.24596609135492856 64-65 0.13690361855759844 66-67 0.10528557317868907 68-69 0.10999770508385077 70-71 0.08971035856086455 72-73 0.07384738016257214 74-75 0.14283874652974868 76-77 0.21611059913138503 78-79 0.139385581164134 80-81 0.08769601209758933 82-83 0.09021394517668337 84-85 0.10006985465770858 86-87 0.07560993331793797 88-89 0.09668863023721089 90-91 0.11632850825414433 92-93 0.16582387849462132 94-95 0.132874925631048 96-97 0.09280381920089438 98-99 0.0812572975096203 100-101 0.06471088013271666 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1390029.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.90873953342382 #Duplication Level Percentage of deduplicated Percentage of total 1 68.25121951876015 34.063323378003325 2 15.444771012441786 15.41658107226663 3 6.388710278980807 9.565574318044815 4 3.18472026481168 6.357814967332108 5 1.8699611536145067 4.666370207668357 6 1.1771296451068114 3.524943411282448 7 0.7956574164461896 2.779718112867487 8 0.5669360132389796 2.2636049453489555 9 0.415894381560403 1.868108792644128 >10 1.8280739401634087 15.135652175040942 >50 0.05284917979727328 1.7640400205005808 >100 0.022916866036547925 2.146402806348416 >500 0.001015287911276904 0.3429752910270601 >1k 1.4504113018241484E-4 0.10489050162478052 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1449 0.10424242947449297 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.194094511697237E-5 0.0 10-11 0.0 0.0 0.0 1.4388189023394475E-4 0.0 12-13 0.0 0.0 0.0 2.1582283535091714E-4 0.0 14-15 0.0 0.0 0.0 4.3164567070183427E-4 0.0 16-17 0.0 0.0 0.0 0.0018344941004827956 0.0 18-19 0.0 0.0 0.0 0.0033812244204977017 0.0 20-21 0.0 0.0 0.0 0.0066905078958784305 0.0 22-23 0.0 0.0 0.0 0.015575214617824521 0.0 24-25 0.0 0.0 0.0 0.025467094571408222 0.0 26-27 0.0 0.0 0.0 0.03028713789424537 0.0 28-29 0.0 0.0 0.0 0.03575464972313527 0.0 30-31 0.0 0.0 0.0 0.04640190960044718 0.0 32-33 0.0 0.0 0.0 0.0726603545681421 0.0 34-35 0.0 0.0 0.0 0.11140055351363173 0.0 36-37 0.0 0.0 0.0 0.17719054782310295 0.0 38-39 0.0 0.0 0.0 0.28114521351712807 0.0 40-41 0.0 0.0 0.0 0.4043081115573848 0.0 42-43 0.0 0.0 0.0 0.5217517044608422 0.0 44-45 0.0 0.0 0.0 0.6694824352585449 0.0 46-47 0.0 0.0 0.0 0.8552339555505677 0.0 48-49 0.0 0.0 0.0 1.0451220801868162 0.0 50-51 0.0 0.0 0.0 1.247455988328301 0.0 52-53 0.0 0.0 0.0 1.449286309853967 0.0 54-55 0.0 0.0 0.0 1.6662242298541972 0.0 56-57 0.0 0.0 0.0 1.9366502425488963 0.0 58-59 0.0 0.0 0.0 2.2319318517815097 0.0 60-61 0.0 0.0 0.0 2.5369614590774727 0.0 62-63 0.0 0.0 0.0 2.835084735642206 0.0 64-65 0.0 0.0 0.0 3.1429560102702894 0.0 66-67 0.0 0.0 0.0 3.50150968073328 0.0 68-69 0.0 0.0 0.0 3.924846172274104 0.0 70-71 0.0 0.0 0.0 4.3801604139194215 0.0 72-73 0.0 0.0 0.0 4.83666168115917 0.0 74-75 0.0 0.0 0.0 5.288954403109575 0.0 76-77 0.0 0.0 0.0 5.787685005132986 0.0 78-79 0.0 0.0 0.0 6.359183873142215 0.0 80-81 0.0 0.0 0.0 6.976581064136072 0.0 82-83 0.0 0.0 0.0 7.612431107552432 0.0 84-85 0.0 0.0 0.0 8.23910868046638 0.0 86-87 0.0 0.0 0.0 8.903843013347203 0.0 88-89 0.0 0.0 0.0 9.640158586619416 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTACA 130 9.926724E-6 25.60877 2 GTACATG 4565 0.0 25.553207 1 ACATGGG 4285 0.0 24.621086 3 TACATGG 4595 0.0 24.115952 2 ACCGTGT 280 6.548362E-11 22.055563 8 GCGTTAC 135 3.7363707E-4 21.16107 1 TACACCG 180 5.141841E-6 21.12444 5 CATGGGG 2350 0.0 21.034546 4 GTATTAT 460 0.0 20.701046 1 GCTACAC 885 0.0 20.405413 3 TAAGGCG 145 6.120847E-4 19.66758 5 ATGGGCG 170 7.9168836E-5 19.57117 5 ACACCGT 305 4.5620254E-9 18.689537 6 CCGTGTA 180 1.2327006E-4 18.47389 9 CATGGGA 1900 0.0 18.011362 4 GTATCAA 3885 0.0 17.647842 1 GGTATCA 3130 0.0 17.341259 1 TACACTG 1455 0.0 17.313328 5 GTATAGG 835 0.0 17.106253 1 GAGTACA 3265 0.0 16.915892 1 >>END_MODULE