FastQCFastQC Report
Fri 10 Feb 2017
SRR2031592_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031592_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390029
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16950.12193990197326818No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTACA908.9465553E-426.3898682
GTACATG43800.022.890681
TATACTG9500.021.9976545
TACGGAG1100.002879450321.5886045
TACATGG45600.021.0424482
GAGTACA33000.020.8787461
CGTATAG1604.9763952E-520.7887521
GTATAGG6200.020.6929511
ACATGGG44850.020.6529393
GGACCGA1150.00372596920.649976
TAATACT13350.020.281654
AGTACAT33950.020.1480242
GTATTAT5200.020.1034091
CGAATCG600.004768114519.78955360-61
TCGTCCG857.965569E-519.55673610-11
CATGGGG23400.018.4729084
CGTCCGA901.2351303E-418.4702510-11
GTATCAA42500.018.4478341
GTATATA13550.017.8846841
TCAACGC42550.017.7503644
Produced by FastQC (version 0.11.5)