##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031592_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1390029 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49246742334153 34.0 31.0 34.0 31.0 34.0 2 32.70649461270232 34.0 31.0 34.0 31.0 34.0 3 32.82201162709555 34.0 31.0 34.0 31.0 34.0 4 36.26217366688033 37.0 37.0 37.0 35.0 37.0 5 35.943949370840464 37.0 35.0 37.0 35.0 37.0 6 36.00518406450513 37.0 37.0 37.0 35.0 37.0 7 35.97474872826394 37.0 36.0 37.0 35.0 37.0 8 36.08873052288837 37.0 37.0 37.0 35.0 37.0 9 37.99703891069898 39.0 39.0 39.0 35.0 39.0 10-11 37.97155491000547 39.0 38.5 39.0 35.0 39.0 12-13 37.9878667999013 39.0 39.0 39.0 35.0 39.0 14-15 39.47149376020212 41.0 39.0 41.0 36.5 41.0 16-17 39.446518022285865 41.0 39.0 41.0 36.0 41.0 18-19 39.45257257222691 41.0 39.5 41.0 36.5 41.0 20-21 39.468778708933414 41.0 39.0 41.0 36.5 41.0 22-23 39.413378066213006 41.0 39.0 41.0 36.0 41.0 24-25 39.350951311087755 41.0 39.0 41.0 36.0 41.0 26-27 39.25781548442515 41.0 39.0 41.0 36.0 41.0 28-29 39.189260439890106 41.0 39.0 41.0 36.0 41.0 30-31 39.11382280513572 40.5 39.0 41.0 36.0 41.0 32-33 38.999062249780394 40.0 39.0 41.0 35.0 41.0 34-35 38.90047509800155 40.0 38.0 41.0 35.0 41.0 36-37 38.788451176198485 40.0 38.0 41.0 35.0 41.0 38-39 38.699289367344136 40.0 38.0 41.0 35.0 41.0 40-41 38.61307749694431 40.0 38.0 41.0 34.5 41.0 42-43 38.49525621407899 40.0 38.0 41.0 34.0 41.0 44-45 38.466838101938876 40.0 38.0 41.0 34.0 41.0 46-47 38.51969779047775 40.0 38.0 41.0 34.5 41.0 48-49 38.66214230062826 40.0 38.0 41.0 35.0 41.0 50-51 38.60264426137872 40.0 38.0 41.0 34.5 41.0 52-53 38.53653053281622 40.0 38.0 41.0 34.0 41.0 54-55 38.45861848925454 40.0 38.0 41.0 34.0 41.0 56-57 38.37273071281247 40.0 38.0 41.0 34.0 41.0 58-59 38.21094775720506 40.0 37.5 41.0 34.0 41.0 60-61 37.968196706687415 40.0 37.0 41.0 33.0 41.0 62-63 37.81804876013378 40.0 36.5 41.0 33.0 41.0 64-65 37.56761153903983 39.0 36.0 41.0 33.0 41.0 66-67 37.32667951531947 39.0 36.0 41.0 33.0 41.0 68-69 37.072190939901255 39.0 35.0 41.0 32.0 41.0 70-71 36.76128339768451 38.0 35.0 40.5 32.0 41.0 72-73 36.41227125477238 37.0 35.0 40.0 32.0 41.0 74-75 36.05718297963568 37.0 35.0 39.0 31.0 41.0 76-77 35.39435400268627 36.0 34.5 39.0 31.0 40.0 78-79 35.31416898496362 36.0 35.0 39.0 31.0 39.5 80-81 34.98625244509287 35.5 35.0 37.0 31.0 39.0 82-83 34.65373168473464 35.0 34.5 37.0 31.0 39.0 84-85 34.32486984084505 35.0 34.0 36.5 31.0 38.0 86-87 34.04174553192775 35.0 34.0 36.0 30.0 37.0 88-89 33.78422752331066 35.0 34.0 36.0 30.0 37.0 90-91 33.593526825699314 35.0 34.0 35.5 30.0 36.5 92-93 33.446238891418815 35.0 34.0 35.0 30.0 36.0 94-95 33.304767382551006 35.0 34.0 35.0 29.5 36.0 96-97 33.20303964881308 35.0 34.0 35.0 29.0 36.0 98-99 33.12167911604722 35.0 34.0 35.0 29.0 35.5 100-101 32.69741746395219 34.5 33.5 35.0 28.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.13845220770053857 1101 2 0.18432740943689652 1101 3 0.1630588937105628 1101 4 0.13885425774859073 1101 5 0.24350332352401693 1101 6 0.04091682243584671 1101 7 0.2898035690442171 1101 8 0.1541334928282012 1101 9 0.3262299751422191 1101 10-11 0.14864994178891777 1101 12-13 0.16308293763532333 1101 14-15 0.15266911845275644 1101 16-17 0.2739182704294265 1101 18-19 0.23340135810067153 1101 20-21 0.15687173528264253 1101 22-23 0.20154102788964678 1101 24-25 0.19891365790143567 1101 26-27 0.22716261122020143 1101 28-29 0.18311272378388566 1101 30-31 0.15810115632352506 1101 32-33 0.12445036951852018 1101 34-35 0.09821372217483315 1101 36-37 0.22754401019395942 1101 38-39 0.28057313144962137 1101 40-41 0.09773378164232582 1101 42-43 0.11202924793317948 1101 44-45 0.1623466100086901 1101 46-47 0.2809645456986587 1101 48-49 0.3534978027240925 1101 50-51 0.3438149933871557 1101 52-53 0.2579965326990461 1101 54-55 0.3977056862659438 1101 56-57 0.33835196761751263 1101 58-59 0.24070519186240347 1101 60-61 0.19695979722722257 1101 62-63 0.17533091478907892 1101 64-65 0.26765212267613947 1101 66-67 0.3364665094050139 1101 68-69 0.19988944587305468 1101 70-71 0.21351114542432015 1101 72-73 0.07544670766336736 1101 74-75 0.05964822891385779 1101 76-77 -0.012139362270332299 1101 78-79 0.2453529317460763 1101 80-81 0.16783566952439344 1101 82-83 0.10862935867189094 1101 84-85 -0.007778015275100358 1101 86-87 0.1325800859599653 1101 88-89 0.10007730428130657 1101 90-91 0.08685001687020844 1101 92-93 -0.06206645854212667 1101 94-95 0.09302323987395056 1101 96-97 -0.05034737953716473 1101 98-99 -0.04970106768899285 1101 100-101 0.026241787633288993 1102 1 0.2560826275494108 1102 2 0.2743140744872079 1102 3 0.11047878299007152 1102 4 0.15416116363617505 1102 5 0.13282728198363003 1102 6 0.06816861053137302 1102 7 0.25251015974208 1102 8 0.12917289154319178 1102 9 0.13994880974843937 1102 10-11 0.2168076982846543 1102 12-13 0.13049110832267985 1102 14-15 0.23930184161111612 1102 16-17 0.13461451643905065 1102 18-19 0.21459907903654596 1102 20-21 0.23425485242815824 1102 22-23 0.13550069976906798 1102 24-25 0.2598725620110258 1102 26-27 0.17479868832045042 1102 28-29 -0.05379680060291747 1102 30-31 -0.12774965387568926 1102 32-33 0.17527673230997465 1102 34-35 0.05710368863541504 1102 36-37 0.17716012527861125 1102 38-39 0.17342681068346621 1102 40-41 0.1857606131774645 1102 42-43 0.3061618700691966 1102 44-45 0.22749592103628657 1102 46-47 0.2805584358671709 1102 48-49 0.2868553814204944 1102 50-51 0.3658217012709457 1102 52-53 0.28711319330437135 1102 54-55 -0.010658963994693238 1102 56-57 -0.00969223976115785 1102 58-59 0.0021490032334838816 1102 60-61 0.18577662350919866 1102 62-63 -0.05559202536529284 1102 64-65 0.09670210853300887 1102 66-67 -0.0010451081647957494 1102 68-69 0.03294799842488061 1102 70-71 0.12945449209259863 1102 72-73 0.17254130498829312 1102 74-75 0.13568572840874538 1102 76-77 0.08899410845339872 1102 78-79 -0.08278179165473176 1102 80-81 -0.07159133172828547 1102 82-83 -0.21852140707749612 1102 84-85 -0.18579757320132018 1102 86-87 -0.21126361226608736 1102 88-89 -0.08735955675235374 1102 90-91 0.0684962770229518 1102 92-93 -0.008950929387083306 1102 94-95 0.015470405186917446 1102 96-97 0.15511388849532892 1102 98-99 0.11442589719563756 1102 100-101 -0.043403017797672305 1103 1 0.12539096075581568 1103 2 0.09924972099231155 1103 3 0.17184079234227312 1103 4 0.12668031736868102 1103 5 0.07093673623553087 1103 6 0.1052697331000303 1103 7 0.14307418575764785 1103 8 0.14929535357843804 1103 9 0.2152797161807385 1103 10-11 0.19813909212804504 1103 12-13 0.10532470313175679 1103 14-15 0.1968285349838439 1103 16-17 0.19386694968657991 1103 18-19 0.26284516070836617 1103 20-21 0.059974164569261745 1103 22-23 0.033925752698870326 1103 24-25 0.08405500607299388 1103 26-27 0.12235630183392487 1103 28-29 0.21134188308252533 1103 30-31 0.2036441895130423 1103 32-33 0.20603541812783277 1103 34-35 0.276110436232635 1103 36-37 0.3590398565932489 1103 38-39 0.3897802141836877 1103 40-41 0.1998978193980463 1103 42-43 0.17586698636243625 1103 44-45 0.33295035571558884 1103 46-47 0.2638082492801104 1103 48-49 0.1659271679344414 1103 50-51 0.24631383415139396 1103 52-53 0.21267866632160093 1103 54-55 0.240039404701335 1103 56-57 0.22920697276531143 1103 58-59 0.3236019452163319 1103 60-61 0.26344177209342234 1103 62-63 0.006824010459425267 1103 64-65 0.20417472973844042 1103 66-67 0.22184874283973244 1103 68-69 0.07607245579925603 1103 70-71 0.22902800739274198 1103 72-73 0.20109293248437154 1103 74-75 0.15631159470579803 1103 76-77 0.08022147049320694 1103 78-79 0.2201358206402091 1103 80-81 0.3252854294250511 1103 82-83 0.22223682179247817 1103 84-85 0.026402935534001415 1103 86-87 0.0723906000319019 1103 88-89 0.14277844151123986 1103 90-91 0.19411429904783972 1103 92-93 0.042307200477509355 1103 94-95 0.12058593684578511 1103 96-97 -0.025919727637976564 1103 98-99 0.03565798103034723 1103 100-101 0.1624771400016627 1104 1 0.04242481044026647 1104 2 0.1202064048706859 1104 3 0.1720200809251793 1104 4 0.07626293354768165 1104 5 0.05084350617241995 1104 6 0.007069790472378656 1104 7 0.03861635443234235 1104 8 0.1152864608647306 1104 9 0.19998568290478147 1104 10-11 0.014945603889380266 1104 12-13 0.11476101982710674 1104 14-15 0.11032322804179273 1104 16-17 0.2849684987399357 1104 18-19 0.22153491974450645 1104 20-21 -0.01618762544794805 1104 22-23 0.03789376761567809 1104 24-25 0.08487256201102156 1104 26-27 0.15673452902841944 1104 28-29 0.11550998405785151 1104 30-31 0.038072617510742646 1104 32-33 0.08470151107103874 1104 34-35 0.24457787742597503 1104 36-37 0.11812620197477486 1104 38-39 0.14246810861856574 1104 40-41 0.16265958072909115 1104 42-43 0.19878723880076166 1104 44-45 0.08347674699499663 1104 46-47 0.17815061875801774 1104 48-49 0.21398296254144356 1104 50-51 0.2622339431588472 1104 52-53 0.23691776557576105 1104 54-55 0.1953255492796515 1104 56-57 0.11012708177276664 1104 58-59 0.07011729231902564 1104 60-61 0.06828399814046548 1104 62-63 0.29797360885299895 1104 64-65 0.12471833272179822 1104 66-67 0.06549618977030747 1104 68-69 0.03202985683196147 1104 70-71 0.12980109976221854 1104 72-73 0.19640561177295268 1104 74-75 0.17320416498691316 1104 76-77 0.17481269252419196 1104 78-79 0.06878900480544559 1104 80-81 0.11937105765224487 1104 82-83 0.08192844542988098 1104 84-85 0.15931673358334564 1104 86-87 0.10538830513804243 1104 88-89 0.1689015051945475 1104 90-91 0.012917368468379209 1104 92-93 0.04381824465421147 1104 94-95 -0.10481351811692008 1104 96-97 -0.049085964012050454 1104 98-99 0.08645760811008785 1104 100-101 0.1278285456241548 1105 1 0.10847923220897826 1105 2 0.12471320759177473 1105 3 0.05975817616327106 1105 4 0.08202823967013018 1105 5 0.031880921138409235 1105 6 0.06933169523428262 1105 7 0.1110653340241825 1105 8 0.12458918195316215 1105 9 0.18874418630613832 1105 10-11 0.1426411821206699 1105 12-13 0.1618528565617936 1105 14-15 0.030484792667643035 1105 16-17 0.025376662005236028 1105 18-19 0.08798900137716004 1105 20-21 0.12013890516429626 1105 22-23 0.07632063836397407 1105 24-25 0.19966848037837082 1105 26-27 0.15043350862025306 1105 28-29 0.12982971194900728 1105 30-31 -0.001408675006260296 1105 32-33 0.14541579678532202 1105 34-35 0.21951835361645777 1105 36-37 0.23759899109041527 1105 38-39 0.10699191814237707 1105 40-41 0.08994699569507247 1105 42-43 0.20218859934497857 1105 44-45 0.1926774272671068 1105 46-47 0.20703667317979324 1105 48-49 0.24802207818770228 1105 50-51 0.25799074587993687 1105 52-53 0.2523344322424279 1105 54-55 0.28927963091229714 1105 56-57 0.14761857837140724 1105 58-59 0.1387482446999826 1105 60-61 0.15794386208604294 1105 62-63 0.1883902755196658 1105 64-65 0.09126595176941521 1105 66-67 0.0825199992941208 1105 68-69 0.14884108132175555 1105 70-71 0.17968205214317123 1105 72-73 0.20205187027635674 1105 74-75 0.29538953913657906 1105 76-77 -0.037177103394178346 1105 78-79 0.06965635174422147 1105 80-81 0.07572309846857195 1105 82-83 0.05123116651832049 1105 84-85 -0.005062518117334491 1105 86-87 0.10032027792716036 1105 88-89 0.19956477050067178 1105 90-91 0.08204151812824279 1105 92-93 0.08831654397394573 1105 94-95 -0.007968280021678709 1105 96-97 0.1439327434709483 1105 98-99 -0.014418242230043177 1105 100-101 0.11444419188266153 1106 1 0.1475954141148108 1106 2 0.16103973820401762 1106 3 0.1401631255445963 1106 4 0.16128261858705173 1106 5 0.12199180013567457 1106 6 -0.028396088792717933 1106 7 0.12074233868431605 1106 8 0.19426940049727648 1106 9 0.21954605035884356 1106 10-11 0.20423366163215917 1106 12-13 0.14131613793733777 1106 14-15 0.09241312305491789 1106 16-17 0.13174015228324265 1106 18-19 0.11865106147679683 1106 20-21 0.21412733518197768 1106 22-23 0.17506240278628127 1106 24-25 0.20869145964882563 1106 26-27 0.17294515894968043 1106 28-29 0.20749161135444183 1106 30-31 0.15884361488606658 1106 32-33 0.20686686540174293 1106 34-35 0.10941711627111772 1106 36-37 0.21559995525561249 1106 38-39 0.23815052331673314 1106 40-41 0.2082468510702924 1106 42-43 0.23671374798711042 1106 44-45 0.28997937166690946 1106 46-47 0.4248271279443756 1106 48-49 0.4042618339325159 1106 50-51 0.46573459932682937 1106 52-53 0.3115470306676187 1106 54-55 0.22387869888594736 1106 56-57 0.23445450959428626 1106 58-59 0.3853174235526211 1106 60-61 0.2582315047021453 1106 62-63 0.1653194093779362 1106 64-65 0.3173626896771822 1106 66-67 0.30029277769681784 1106 68-69 0.13908694344613082 1106 70-71 0.22459768768872834 1106 72-73 0.19992267214040993 1106 74-75 0.30698041170607837 1106 76-77 0.1440843460674941 1106 78-79 0.23750947724638394 1106 80-81 0.3081801127703585 1106 82-83 0.2882342197080945 1106 84-85 0.2207176522185179 1106 86-87 0.09422032613542086 1106 88-89 0.18470859180872168 1106 90-91 0.2528353474709988 1106 92-93 0.1246286121082889 1106 94-95 0.10154803043950977 1106 96-97 0.07827952155225404 1106 98-99 0.285588185537911 1106 100-101 0.3805778894561769 1107 1 0.10703377019729032 1107 2 0.12208757419490723 1107 3 0.1329635129676987 1107 4 0.11132177940948651 1107 5 0.13203371118380858 1107 6 0.0629448853850576 1107 7 0.14858598237464093 1107 8 0.06879633178348143 1107 9 0.06178712557751709 1107 10-11 0.022780645650954057 1107 12-13 -0.007868061418150774 1107 14-15 0.02949274208886976 1107 16-17 -0.011736132998869664 1107 18-19 -0.009048793239543329 1107 20-21 -0.012011467513254104 1107 22-23 0.0791551571373148 1107 24-25 0.1690980745394981 1107 26-27 0.1297764804331223 1107 28-29 -0.07627817083964317 1107 30-31 -0.041096896536330974 1107 32-33 0.12131123012949985 1107 34-35 0.06767108167806413 1107 36-37 0.10357608807955643 1107 38-39 0.09291229996252781 1107 40-41 0.06183858604419612 1107 42-43 0.04442125550539089 1107 44-45 0.08011114335034364 1107 46-47 0.15250426340494982 1107 48-49 0.008429621615093197 1107 50-51 0.060847268899166806 1107 52-53 -0.07058128529744323 1107 54-55 -0.048760441608742155 1107 56-57 -0.1489323335651278 1107 58-59 -0.11143073349721533 1107 60-61 0.04933183413135822 1107 62-63 -0.13217160754791024 1107 64-65 -0.10469890342095312 1107 66-67 -0.004984068392182905 1107 68-69 0.0043599631341564304 1107 70-71 -0.004331398339523673 1107 72-73 0.1119181402467575 1107 74-75 -0.015050390821734538 1107 76-77 -0.0518976339754289 1107 78-79 0.0662472432260941 1107 80-81 -0.02507370316780566 1107 82-83 0.08161903009198568 1107 84-85 0.007679489847582488 1107 86-87 -0.19418268954684237 1107 88-89 -0.10624466033316793 1107 90-91 0.0603347944364927 1107 92-93 0.11041562506423475 1107 94-95 0.10783169828399508 1107 96-97 -0.026917209266414943 1107 98-99 -0.006238861138200491 1107 100-101 0.16974768002050666 1108 1 0.1045513740900077 1108 2 0.17126784710762166 1108 3 0.09747077996858877 1108 4 0.09256720139168095 1108 5 0.10716616989574845 1108 6 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-0.9353633095110254 2316 80-81 -1.1819193083326809 2316 82-83 -1.4171682535295744 2316 84-85 -1.280454974346334 2316 86-87 -0.8929557924579328 2316 88-89 -0.8067147488420545 2316 90-91 -0.5328087707494191 2316 92-93 -0.3662387162356353 2316 94-95 -0.4822924381025686 2316 96-97 -0.4225040659137349 2316 98-99 -0.44673892938542537 2316 100-101 -0.6754428931989551 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 1.0 11 1.0 12 1.0 13 11.0 14 66.0 15 158.0 16 317.0 17 550.0 18 936.0 19 1336.0 20 1742.0 21 2282.0 22 2891.0 23 3312.0 24 3877.0 25 4729.0 26 6015.0 27 8041.0 28 10343.0 29 12974.0 30 16568.0 31 20990.0 32 26982.0 33 35236.0 34 50214.0 35 76406.0 36 134494.0 37 271794.0 38 480037.0 39 217589.0 40 136.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.81798291228204 21.03353991848391 14.671816061245742 22.476661107988303 2 14.150565359305498 20.731208107044527 43.29924942500041 21.818977108649563 3 18.120275707668146 26.199710364504757 31.231425667057305 24.448588260769785 4 11.642969279494865 17.092409017717998 40.91522614084896 30.349395561938174 5 11.897953208170478 39.23349800615671 36.92577636869447 11.94277241697835 6 29.3763655290645 39.85880870111343 18.343789949706085 12.421035820115984 7 26.333479373451922 33.45606458570289 23.31267908799025 16.89777695285494 8 23.56389686833872 38.9308424500496 20.925678529009105 16.57958215260257 9 24.793439561332892 17.237985682313102 21.29013135697169 36.67844339938232 10-11 23.57357292545695 27.497088190246387 30.063293643513912 18.866045240782746 12-13 23.83712857789298 24.594235084303996 30.229081551535973 21.339554786267048 14-15 20.950138450348877 26.888827499282392 27.157850663547308 25.003183386821426 16-17 20.983698901245944 30.297245597034305 27.877691760387734 20.841363741332014 18-19 20.962620204326672 29.681107372579994 28.982992441164896 20.373279981928434 20-21 21.764078303402304 28.672531292512605 28.867958869922855 20.695431534162235 22-23 21.565105786313694 28.34052340457217 28.749229763693478 21.34514104542065 24-25 21.446530971655985 28.529152988894474 28.609582965535253 21.414733073914284 26-27 21.460019898865422 28.608288028523145 28.709436997357606 21.222255075253827 28-29 21.33085707028387 28.602205394794737 28.754429372355823 21.312508162565567 30-31 21.715417030482065 28.366735993961655 28.560327158635022 21.357519816921254 32-33 21.534982363677305 28.572461437854894 28.555807109060318 21.336749089407487 34-35 21.693432295297434 28.456492634326334 28.6597977452269 21.19027732514933 36-37 21.832494142208546 28.34886898043134 28.398903907760197 21.419732969599913 38-39 21.489839420616406 28.408579964878435 28.640841306188573 21.46073930831659 40-41 21.46760966857526 28.424514884221843 28.68019300316756 21.42768244403534 42-43 21.60760674777289 28.322394712628295 28.431061510227483 21.63893702937133 44-45 21.655195682967765 28.2814962853293 28.357358011955146 21.70595001974779 46-47 21.84091123278723 28.17167843260824 28.26099311597096 21.726417218633568 48-49 21.727528079779894 28.126594522005032 28.465127961178045 21.680749437037026 50-51 21.284147422466788 28.284261103308545 28.65314823981765 21.778443234407018 52-53 21.489947332034077 28.192325483856813 28.593827898554636 21.723899285554474 54-55 21.478557988340427 28.20207529910616 28.512862976806392 21.80650373574702 56-57 21.749294439180765 28.043155934156772 28.21239700754445 21.99515261911802 58-59 21.483220853665642 27.929417299926833 28.49670762264672 22.0906542237608 60-61 21.681569838316264 28.013670223308374 28.600204959826364 21.704554978549 62-63 21.206068362602508 28.337754104410774 28.432931974800525 22.023245558186197 64-65 21.37894245371859 28.28458974596933 28.191785926768436 22.144681873543647 66-67 21.466458613453387 28.23232464934185 28.07686026694407 22.22435647026069 68-69 21.389050156507526 27.99988345566891 28.280668964460453 22.33039742336311 70-71 21.49796874741462 27.82643383699189 28.30707129131838 22.368526124275103 72-73 21.469084457950157 28.00060286512008 28.069306467706788 22.461006209222973 74-75 21.241607189490292 28.05434275112246 28.02999074120036 22.674059318186888 76-77 21.228262144171094 27.822836789736044 28.19455565315544 22.754345412937425 78-79 21.390139465017203 27.708870193794937 28.08048194781968 22.820508393368183 80-81 21.34430038122531 27.85106850651558 27.890317049804636 22.91431406245447 82-83 21.29805361025297 27.79098361245448 27.910195847644314 23.000766929648233 84-85 21.302145494806226 27.715680751984312 28.052760050329884 22.929413702879582 86-87 20.987583712282262 27.790895010104105 28.086536324062305 23.13498495355133 88-89 21.045136468375837 27.601078826412973 27.939165297990186 23.414619407221 90-91 21.287829246727945 27.550720164831095 27.848231943362332 23.313218645078628 92-93 21.30455551646764 27.60924412368375 27.806434254249375 23.279766105599236 94-95 21.203694311413646 27.230834752368477 27.919669301863486 23.64580163435439 96-97 20.94506661371813 27.300401646296592 28.010926390744366 23.743605349240916 98-99 21.040712100251145 27.388205569811852 27.798017163670686 23.773065166266317 100-101 21.046827080586088 27.350904189768706 27.595143698440822 24.007125031204385 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 5.0 1 4.5 2 14.5 3 20.0 4 10.5 5 12.0 6 27.5 7 50.0 8 103.0 9 136.0 10 117.0 11 113.5 12 127.0 13 132.0 14 142.5 15 157.0 16 166.5 17 179.0 18 195.5 19 221.0 20 248.5 21 299.5 22 346.0 23 411.5 24 518.0 25 634.0 26 812.0 27 1050.0 28 1363.5 29 2124.0 30 3974.5 31 7695.5 32 14971.5 33 25705.0 34 38790.5 35 55547.0 36 73113.5 37 85607.5 38 89490.0 39 86598.0 40 82008.0 41 75585.0 42 68214.5 43 61142.5 44 53488.0 45 46678.0 46 41455.5 47 38964.0 48 39191.0 49 37767.0 50 34913.5 51 33043.0 52 32057.5 53 31645.5 54 30619.0 55 28926.5 56 26787.5 57 24137.5 58 21407.5 59 18966.0 60 16404.0 61 13557.0 62 10930.0 63 8430.5 64 6111.5 65 4414.0 66 3081.0 67 2173.0 68 1620.0 69 1162.5 70 882.0 71 739.5 72 588.0 73 496.0 74 399.0 75 288.5 76 199.5 77 119.0 78 67.0 79 41.0 80 31.0 81 26.5 82 13.0 83 6.0 84 6.5 85 6.0 86 4.0 87 1.5 88 1.0 89 1.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04388397652135315 2 0.0020143464632752265 3 4.316456707018343E-4 4 0.01028755515172705 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.002410021661418575 24-25 0.0 26-27 0.0 28-29 0.0216182540076502 30-31 0.01758956108109975 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03640211822918802 50-51 0.012445783505236221 52-53 0.0 54-55 8.992618139621547E-4 56-57 0.0 58-59 0.0 60-61 3.5970472558486194E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 2.1582283535091716E-4 80-81 0.012122049252209846 82-83 0.005107807103305039 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1390029.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.60011169250848 #Duplication Level Percentage of deduplicated Percentage of total 1 66.71487227762536 32.423502442440295 2 16.349515636765243 15.891765721304097 3 6.577866206090015 9.590550969430552 4 3.310175383310411 6.4349957340271216 5 1.95460001543709 4.749688953221068 6 1.2349914495812488 3.6012433433364985 7 0.847735743501123 2.884003627392041 8 0.5957220450937147 2.3161726343395284 9 0.44417621525437834 1.9428312305266628 >10 1.8844289447147173 15.402730331720512 >50 0.05854390745439839 1.8935422545621174 >100 0.025876450069456228 2.2817634934944078 >500 0.0011965800822312971 0.37727359262499977 >1k 2.991450205578243E-4 0.20993567158015403 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1695 0.12193990197326818 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 7.194094511697237E-5 0.0 10-11 0.0 0.0 0.0 1.4388189023394475E-4 0.0 12-13 0.0 0.0 0.0 2.1582283535091714E-4 0.0 14-15 0.0 0.0 0.0 4.3164567070183427E-4 0.0 16-17 0.0 0.0 0.0 0.001906435045599768 0.0 18-19 0.0 0.0 0.0 0.00348913583817316 0.0 20-21 0.0 0.0 0.0 0.006654537423319945 0.0 22-23 0.0 0.0 0.0 0.015467303200149062 0.0 24-25 0.0 0.0 0.0 0.025071419373264875 0.0 26-27 0.0 0.0 0.0 0.029711610333309595 0.0 28-29 0.0 0.0 0.0 0.03532300405243344 0.0 30-31 0.0 0.0 0.0 0.04632996865533021 0.0 32-33 0.0 0.0 0.0 0.07273229551325908 0.0 34-35 0.0 0.0 0.0 0.11158040587642415 0.0 36-37 0.0 0.0 0.0 0.177118606877986 0.0 38-39 0.0 0.0 0.0 0.281289095407362 0.0 40-41 0.0 0.0 0.0 0.40517140289878845 0.0 42-43 0.0 0.0 0.0 0.5236221690338835 0.0 44-45 0.0 0.0 0.0 0.6718205159748466 0.0 46-47 0.0 0.0 0.0 0.8572483020138428 0.0 48-49 0.0 0.0 0.0 1.0475680723207934 0.0 50-51 0.0 0.0 0.0 1.250405567078097 0.0 52-53 0.0 0.0 0.0 1.4535667960884269 0.0 54-55 0.0 0.0 0.0 1.6726269739696078 0.0 56-57 0.0 0.0 0.0 1.9450313626550235 0.0 58-59 0.0 0.0 0.0 2.2410323813388064 0.0 60-61 0.0 0.0 0.0 2.546889309503615 0.0 62-63 0.0 0.0 0.0 2.846487375443246 0.0 64-65 0.0 0.0 0.0 3.1547183547969144 0.0 66-67 0.0 0.0 0.0 3.514423080381776 0.0 68-69 0.0 0.0 0.0 3.9397379479133168 0.0 70-71 0.0 0.0 0.0 4.396491008460974 0.0 72-73 0.0 0.0 0.0 4.856409470593778 0.0 74-75 0.0 0.0 0.0 5.311435948458629 0.0 76-77 0.0 0.0 0.0 5.8125406016709 0.0 78-79 0.0 0.0 0.0 6.387888310243887 0.0 80-81 0.0 0.0 0.0 7.008918518966151 0.0 82-83 0.0 0.0 0.0 7.651351158860715 0.0 84-85 0.0 0.0 0.0 8.282525040844472 0.0 86-87 0.0 0.0 0.0 8.951395978069522 0.0 88-89 0.0 0.0 0.0 9.69209994899387 3.597047255848619E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTTACA 90 8.9465553E-4 26.389868 2 GTACATG 4380 0.0 22.89068 1 TATACTG 950 0.0 21.997654 5 TACGGAG 110 0.0028794503 21.588604 5 TACATGG 4560 0.0 21.042448 2 GAGTACA 3300 0.0 20.878746 1 CGTATAG 160 4.9763952E-5 20.788752 1 GTATAGG 620 0.0 20.692951 1 ACATGGG 4485 0.0 20.652939 3 GGACCGA 115 0.003725969 20.64997 6 TAATACT 1335 0.0 20.28165 4 AGTACAT 3395 0.0 20.148024 2 GTATTAT 520 0.0 20.103409 1 CGAATCG 60 0.0047681145 19.789553 60-61 TCGTCCG 85 7.965569E-5 19.556736 10-11 CATGGGG 2340 0.0 18.472908 4 CGTCCGA 90 1.2351303E-4 18.47025 10-11 GTATCAA 4250 0.0 18.447834 1 GTATATA 1355 0.0 17.884684 1 TCAACGC 4255 0.0 17.750364 4 >>END_MODULE