FastQCFastQC Report
Fri 10 Feb 2017
SRR2031591_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031591_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984206
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13450.037.1932221
GTATCAA20200.031.8404481
TCAACGC22450.027.5376224
ACTGCGC1405.7766556E-727.1578858
ATCAACG22900.026.996493
CAACGCA23150.026.7049525
GTACATG27250.026.5750351
TACATGG26300.025.8834722
AACGCAG24150.025.780316
TAACGCT950.001218128125.0291354
ACATGGG26350.024.7251763
TATCAAC26000.024.3512082
TACCTGG8650.024.2146842
GTACCTG9800.022.849071
ACGCAGA27200.022.7136177
TATAGGG2103.36604E-822.6685072
TACACCG1050.002181180322.6454095
CATGGGG18250.021.6279164
CGCAGAG28450.021.2156078
GTACACG1805.034901E-621.1745551
Produced by FastQC (version 0.11.5)