##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031591_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 984206 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.660713305954243 33.0 31.0 34.0 28.0 34.0 2 31.932725466010165 34.0 31.0 34.0 30.0 34.0 3 32.08091801919517 34.0 31.0 34.0 30.0 34.0 4 35.1737075368368 37.0 35.0 37.0 32.0 37.0 5 34.847665021347154 37.0 35.0 37.0 32.0 37.0 6 35.060714931630166 37.0 35.0 37.0 32.0 37.0 7 35.21398467394021 37.0 35.0 37.0 32.0 37.0 8 35.29547472785169 37.0 35.0 37.0 33.0 37.0 9 37.206871325718396 39.0 37.0 39.0 34.0 39.0 10-11 37.15872388504033 39.0 37.0 39.0 34.0 39.0 12-13 37.1675843268584 39.0 37.0 39.0 34.0 39.0 14-15 38.54214361627545 41.0 38.0 41.0 34.0 41.0 16-17 38.486736516542265 40.5 38.0 41.0 34.0 41.0 18-19 38.39832057516414 40.0 38.0 41.0 33.5 41.0 20-21 38.38773742488869 40.0 38.0 41.0 34.0 41.0 22-23 38.32022767591337 40.0 38.0 41.0 33.5 41.0 24-25 38.24072247070227 40.0 38.0 41.0 33.0 41.0 26-27 38.138243416520524 40.0 38.0 41.0 33.0 41.0 28-29 38.023735376537026 40.0 38.0 41.0 33.0 41.0 30-31 37.88361735246483 40.0 38.0 41.0 32.0 41.0 32-33 37.73577025541401 40.0 38.0 41.0 32.0 41.0 34-35 37.60045661172559 40.0 37.5 41.0 31.0 41.0 36-37 37.46621134193451 40.0 37.0 41.0 31.0 41.0 38-39 37.28742661597267 40.0 37.0 41.0 30.5 41.0 40-41 37.135344633135745 40.0 37.0 41.0 30.0 41.0 42-43 36.951833254420315 40.0 36.5 41.0 30.0 41.0 44-45 36.7398186964924 40.0 36.0 41.0 30.0 41.0 46-47 36.54338675033479 39.5 36.0 41.0 29.0 41.0 48-49 36.6987419300431 40.0 36.0 41.0 29.5 41.0 50-51 36.47817682477043 39.5 35.5 40.5 29.5 41.0 52-53 36.604539598417404 39.5 36.0 40.5 29.5 41.0 54-55 36.87714665425734 40.0 36.0 41.0 30.0 41.0 56-57 36.764533542774586 40.0 36.0 41.0 29.0 41.0 58-59 36.563352082795674 39.0 35.5 41.0 29.0 41.0 60-61 36.2579393948015 39.0 35.0 41.0 28.0 41.0 62-63 35.90738371844918 39.0 35.0 41.0 28.0 41.0 64-65 35.62395778932459 38.0 35.0 41.0 27.5 41.0 66-67 35.390157649922884 37.5 35.0 40.0 27.5 41.0 68-69 35.11929667163175 37.0 34.0 40.0 27.0 41.0 70-71 34.807229888864725 36.5 34.0 39.0 26.5 41.0 72-73 34.43078786351638 36.0 34.0 39.0 26.0 41.0 74-75 34.01295104886579 35.5 34.0 38.5 26.0 40.5 76-77 33.61918185826951 35.0 33.5 37.0 26.0 39.5 78-79 33.27495514150493 35.0 33.0 37.0 25.5 39.0 80-81 32.96330544621756 35.0 33.0 36.5 25.0 39.0 82-83 32.65104713850556 35.0 33.0 36.0 25.0 37.5 84-85 32.40671160305871 35.0 33.0 36.0 24.5 37.0 86-87 32.174118528031734 35.0 32.0 35.0 24.0 37.0 88-89 31.933708999945132 35.0 32.0 35.0 23.5 36.0 90-91 31.752908943859314 35.0 32.0 35.0 23.0 36.0 92-93 31.579235444612205 35.0 32.0 35.0 23.0 36.0 94-95 31.429437536450703 35.0 32.0 35.0 21.0 35.5 96-97 31.322777446997883 35.0 31.5 35.0 20.0 35.0 98-99 31.211903300731755 35.0 31.0 35.0 20.0 35.0 100-101 30.742898336323897 34.5 30.5 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33840586657293414 1101 2 0.3506074123776415 1101 3 0.47316860781127446 1101 4 0.2440810340672357 1101 5 0.2979543165431906 1101 6 0.43497795111589 1101 7 0.43808748045317003 1101 8 0.34096541636694155 1101 9 0.484799429692373 1101 10-11 0.4627872440467229 1101 12-13 0.538911402518039 1101 14-15 0.42653055036778653 1101 16-17 0.43110207264560074 1101 18-19 0.48738812620185 1101 20-21 0.14287444805998462 1101 22-23 0.49326103999437976 1101 24-25 0.5418330210441766 1101 26-27 0.5762574733417765 1101 28-29 0.5695181934779399 1101 30-31 0.47199324507344187 1101 32-33 0.5532959055149718 1101 34-35 0.688962977117221 1101 36-37 0.6855160948468324 1101 38-39 0.6085411052849068 1101 40-41 0.6011763575702886 1101 42-43 0.6441613754301017 1101 44-45 0.6495170637330432 1101 46-47 0.41862339368179846 1101 48-49 0.3471372658045908 1101 50-51 0.3917000377121056 1101 52-53 0.26852827315354943 1101 54-55 0.2690437752662689 1101 56-57 0.3539071650688257 1101 58-59 0.2953964426354503 1101 60-61 0.0530378046901987 1101 62-63 0.11719611660878115 1101 64-65 0.24036278719169957 1101 66-67 0.36431143976806624 1101 68-69 0.3657953229278945 1101 70-71 0.44452569370498196 1101 72-73 0.30643627422919195 1101 74-75 0.2122706113490338 1101 76-77 0.3073564561972262 1101 78-79 0.43301446951661404 1101 80-81 0.5480539551002508 1101 82-83 0.45110379093603115 1101 84-85 0.2965997052615279 1101 86-87 0.44745573400266636 1101 88-89 0.4162813197135904 1101 90-91 0.38239994104922914 1101 92-93 0.2811541660954653 1101 94-95 0.1329491524002009 1101 96-97 0.4422297962623247 1101 98-99 0.3381870757140568 1101 100-101 0.5459194521228738 1102 1 0.18662692233004918 1102 2 0.19663911593068661 1102 3 0.24009817515297271 1102 4 0.27152312391733346 1102 5 0.4306433329531387 1102 6 0.3900095743841945 1102 7 0.3435604786550499 1102 8 0.3747449034876098 1102 9 0.45315959469663625 1102 10-11 0.43120730401815166 1102 12-13 0.45789772480138424 1102 14-15 0.29658459601795073 1102 16-17 0.47400725891257167 1102 18-19 0.507143270478629 1102 20-21 0.601440511571596 1102 22-23 0.4201611441096702 1102 24-25 0.546890322686707 1102 26-27 0.5317478665713509 1102 28-29 0.46426362075206384 1102 30-31 0.6139761762821578 1102 32-33 0.41982952889287617 1102 34-35 0.3342076353144776 1102 36-37 0.21198202416822198 1102 38-39 0.4561045907642054 1102 40-41 0.6079113554739664 1102 42-43 0.5523666036235539 1102 44-45 0.11092558946302944 1102 46-47 -0.4206153285419063 1102 48-49 0.23882486385074486 1102 50-51 0.41121687648121963 1102 52-53 0.49072458336870284 1102 54-55 0.45947281438532883 1102 56-57 0.49485850104517937 1102 58-59 0.6559093474521447 1102 60-61 0.43795217400256803 1102 62-63 -0.04000650806514017 1102 64-65 0.23470016407249972 1102 66-67 0.46537463915331756 1102 68-69 0.6587224919221839 1102 70-71 0.5236692466489501 1102 72-73 0.73234173958123 1102 74-75 0.6502684882636061 1102 76-77 0.5890526816673685 1102 78-79 0.6380954742738041 1102 80-81 0.4201304116630169 1102 82-83 0.26939138108114236 1102 84-85 0.2662093812297499 1102 86-87 0.4418663866411592 1102 88-89 0.3464603546909757 1102 90-91 0.4974352344772228 1102 92-93 0.2894617254775049 1102 94-95 0.1491685820726758 1102 96-97 0.2922986754745658 1102 98-99 0.10908882630376837 1102 100-101 0.22958418303517547 1103 1 -0.02970548589516042 1103 2 -0.042869036969605645 1103 3 0.00925844551613153 1103 4 0.19354796510300076 1103 5 0.021586635281181543 1103 6 -0.06617343688586175 1103 7 0.05809871912649811 1103 8 0.3199972264205613 1103 9 0.2270907622175784 1103 10-11 0.26104345763783243 1103 12-13 0.31524260289283035 1103 14-15 0.44962039191273817 1103 16-17 0.4702076636935786 1103 18-19 0.3095489211523841 1103 20-21 0.18061424589187425 1103 22-23 0.26636592190510555 1103 24-25 0.28206734354290575 1103 26-27 0.19250542049185526 1103 28-29 0.215976795351132 1103 30-31 0.3968547777059186 1103 32-33 0.38639136639658034 1103 34-35 0.3058797405329017 1103 36-37 0.30757858340655275 1103 38-39 0.17938762827235166 1103 40-41 0.27831602560701896 1103 42-43 -0.03472105499061229 1103 44-45 0.13438773578778296 1103 46-47 0.24032145737870536 1103 48-49 0.16424833794587101 1103 50-51 0.2147619745784013 1103 52-53 -0.038535194629986336 1103 54-55 -0.10605526714244462 1103 56-57 0.14479807953586743 1103 58-59 0.2667467858757888 1103 60-61 0.2838933390967995 1103 62-63 0.22052598959128744 1103 64-65 0.22657621190219146 1103 66-67 0.20805291956084915 1103 68-69 0.1691191797640954 1103 70-71 0.23347416093170636 1103 72-73 0.14883564138180105 1103 74-75 0.17737440267324445 1103 76-77 -0.22342445239446818 1103 78-79 -0.0911416379599288 1103 80-81 0.03606518353332433 1103 82-83 -0.005501233698169017 1103 84-85 0.08079541690123193 1103 86-87 0.113247286053209 1103 88-89 0.015821660534676596 1103 90-91 0.028225567528462392 1103 92-93 0.11217725657702715 1103 94-95 -0.06048851200379701 1103 96-97 0.10277962083405967 1103 98-99 0.14070387292256825 1103 100-101 -0.12853983072688635 1104 1 0.0917141087895601 1104 2 0.1372075913828894 1104 3 0.08254853314558375 1104 4 -0.19915949251706877 1104 5 -0.23479895878433865 1104 6 -0.03005336214201293 1104 7 0.07452327593145469 1104 8 0.08962635841051281 1104 9 0.27918139953492727 1104 10-11 0.2389256173777241 1104 12-13 0.23993340345231218 1104 14-15 0.17516680639845816 1104 16-17 0.11963295104990124 1104 18-19 0.35280441466613865 1104 20-21 0.3365716658830351 1104 22-23 0.2877550463570344 1104 24-25 0.2574115409214812 1104 26-27 0.36944691886860426 1104 28-29 0.2872171785663227 1104 30-31 0.24493129837244254 1104 32-33 0.10089813409226167 1104 34-35 -0.025583161040906077 1104 36-37 0.23023069288890952 1104 38-39 -0.004123325715148951 1104 40-41 0.030033800276576983 1104 42-43 -0.0579879534268386 1104 44-45 -0.14747782562930922 1104 46-47 0.06670782237782902 1104 48-49 0.08708277934328379 1104 50-51 0.0186639999311069 1104 52-53 0.16009401200591356 1104 54-55 0.12021496833493472 1104 56-57 0.15883702514344122 1104 58-59 0.12377041927361176 1104 60-61 0.06061261280392927 1104 62-63 0.2838900011871104 1104 64-65 0.14169152766068294 1104 66-67 0.16669294374124632 1104 68-69 0.04043873663635367 1104 70-71 0.24762752287524137 1104 72-73 0.22530980715210802 1104 74-75 -0.11739083580833665 1104 76-77 -0.13825709249941553 1104 78-79 -0.06473593843558234 1104 80-81 -0.0387123164636165 1104 82-83 0.030879490024929623 1104 84-85 -0.10696913263492291 1104 86-87 -0.1162822853688894 1104 88-89 -0.154511835551304 1104 90-91 -0.1337757271505069 1104 92-93 -0.06634893864538682 1104 94-95 -0.002401724459637933 1104 96-97 0.1411066459726129 1104 98-99 0.018183556374783194 1104 100-101 0.3543699174225665 1105 1 0.13356168373895372 1105 2 0.22652291950418402 1105 3 0.34574105708880865 1105 4 0.18769806317453686 1105 5 -0.16531375866517806 1105 6 0.0994441291849455 1105 7 0.1977860286459574 1105 8 0.16435995117277713 1105 9 0.3634090120616378 1105 10-11 0.2680157865225752 1105 12-13 0.15556387907982128 1105 14-15 0.36074469254527486 1105 16-17 0.32183622041734594 1105 18-19 0.4007483348577239 1105 20-21 0.333263126073831 1105 22-23 0.1463516203001447 1105 24-25 0.27827492789983665 1105 26-27 0.1981294139837928 1105 28-29 0.2323459664984142 1105 30-31 0.14148811823353213 1105 32-33 0.28047232818544643 1105 34-35 0.40639239259122206 1105 36-37 0.3130092799160593 1105 38-39 0.3182845547317399 1105 40-41 0.2540246428576367 1105 42-43 0.194347727902489 1105 44-45 0.4095032989223739 1105 46-47 0.3160915027501332 1105 48-49 0.19339586267720676 1105 50-51 0.24755842455480348 1105 52-53 0.2992690702366829 1105 54-55 0.1946383488393053 1105 56-57 0.5547335444328994 1105 58-59 0.4192355734649382 1105 60-61 0.4104898180084504 1105 62-63 0.5025960935447458 1105 64-65 0.29989743353912246 1105 66-67 0.3528021196908284 1105 68-69 0.4132182853801396 1105 70-71 0.27193373814495914 1105 72-73 0.27485756576592024 1105 74-75 0.4118922630772417 1105 76-77 0.5229793936225491 1105 78-79 0.5173454040270258 1105 80-81 0.38164757666854854 1105 82-83 0.18476881285913294 1105 84-85 0.35564178730568585 1105 86-87 0.45945700497804864 1105 88-89 0.31448980874958465 1105 90-91 0.2406874121271727 1105 92-93 0.47636359486809354 1105 94-95 0.24934461222093773 1105 96-97 0.3014183594467319 1105 98-99 0.4412403609891449 1105 100-101 0.28085606283881503 1106 1 0.13758258452602234 1106 2 0.03535573953103466 1106 3 0.18819983901691018 1106 4 0.4003495324595079 1106 5 0.43403736929290915 1106 6 0.18771748901717444 1106 7 0.2778399038905448 1106 8 0.3449231843894083 1106 9 0.4596605585619429 1106 10-11 0.3211079096810323 1106 12-13 0.4249861679880169 1106 14-15 0.37349728759472356 1106 16-17 0.3464848029017489 1106 18-19 0.2760411827873952 1106 20-21 0.18831656259493457 1106 22-23 0.13069906298092349 1106 24-25 0.13838574945344817 1106 26-27 0.21012959824766142 1106 28-29 0.3361720740558667 1106 30-31 0.33073478612862317 1106 32-33 0.3460647803865271 1106 34-35 0.31304190516942043 1106 36-37 0.36451541765768525 1106 38-39 0.5865135157467734 1106 40-41 0.4612107142642756 1106 42-43 0.2863261076088932 1106 44-45 0.392849355236919 1106 46-47 0.6567844226304516 1106 48-49 0.37695667414017464 1106 50-51 0.2342091655587808 1106 52-53 0.3142876721126697 1106 54-55 0.4425675739658743 1106 56-57 0.41939502538790663 1106 58-59 0.4000304526843621 1106 60-61 0.21133787051488184 1106 62-63 0.44801011690238823 1106 64-65 0.4566489493287733 1106 66-67 0.289171858299369 1106 68-69 0.41774591179832044 1106 70-71 0.47135221015878415 1106 72-73 0.5039381329476527 1106 74-75 0.4080084633808454 1106 76-77 0.357897615563985 1106 78-79 0.34843686032578347 1106 80-81 0.4404365243463886 1106 82-83 0.5026382399739973 1106 84-85 0.5316192632417014 1106 86-87 0.28563475818730666 1106 88-89 0.43939987043410866 1106 90-91 0.39520911577822915 1106 92-93 0.4366152466620328 1106 94-95 0.27062928234474626 1106 96-97 0.3913358355582375 1106 98-99 0.4501091563421866 1106 100-101 0.37124845712838805 1107 1 0.10773889675222748 1107 2 -0.007169648760484648 1107 3 0.05277902393659417 1107 4 0.24301264808408973 1107 5 0.17359581214436304 1107 6 0.16310909926541228 1107 7 0.2228651262455159 1107 8 0.36376113075871785 1107 9 0.26212969436441114 1107 10-11 0.3020438747679819 1107 12-13 0.319031535039656 1107 14-15 0.20320075898726486 1107 16-17 0.22715983277033303 1107 18-19 0.30155392671412073 1107 20-21 0.21793001341133333 1107 22-23 0.32031475394069275 1107 24-25 0.2861169820301086 1107 26-27 0.28102391326159193 1107 28-29 0.35392521025658397 1107 30-31 0.22299840951904315 1107 32-33 0.2793105821919113 1107 34-35 0.4574544068287878 1107 36-37 0.3798811418047734 1107 38-39 0.32112497532462925 1107 40-41 0.2802278721031186 1107 42-43 0.3289469094142845 1107 44-45 0.2496025578122527 1107 46-47 0.6563218745088548 1107 48-49 0.5644151900037073 1107 50-51 0.5584076920422589 1107 52-53 0.672706462767124 1107 54-55 0.5664454280744593 1107 56-57 0.6129745255870276 1107 58-59 0.6453966746410842 1107 60-61 0.6811228997197176 1107 62-63 0.5671089984900632 1107 64-65 0.6492680917687039 1107 66-67 0.5564956336876605 1107 68-69 0.6552322220332769 1107 70-71 0.6023736325668594 1107 72-73 0.5159623099023847 1107 74-75 0.5743432569557783 1107 76-77 0.6958087233563646 1107 78-79 0.6097412875128256 1107 80-81 0.6848589821985058 1107 82-83 0.7516073591828132 1107 84-85 0.8402156060002355 1107 86-87 0.819674566364661 1107 88-89 0.7618415437382424 1107 90-91 0.6080538489522667 1107 92-93 0.6472509495692442 1107 94-95 0.6047819273732884 1107 96-97 0.7128150768963515 1107 98-99 0.7687280664709526 1107 100-101 0.7063635341229464 1108 1 0.19336143903299075 1108 2 0.04096268443431228 1108 3 0.027918094878764066 1108 4 0.04817445792455999 1108 5 0.18749391470518617 1108 6 0.24419784890574903 1108 7 0.22780054617582124 1108 8 0.20455787138341464 1108 9 0.10008806899275413 1108 10-11 0.07208777402946254 1108 12-13 0.059031814780055925 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-1.3546259362953492 2316 82-83 -1.6428884650623985 2316 84-85 -2.313781495365859 2316 86-87 -1.5906704715184716 2316 88-89 -1.4087742724711525 2316 90-91 -1.5685995920992788 2316 92-93 -1.7052667005283375 2316 94-95 -1.7526415171221785 2316 96-97 -1.6684539364201463 2316 98-99 -1.0671224386375293 2316 100-101 -1.656154044226266 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 4.0 7 52.0 8 172.0 9 217.0 10 276.0 11 389.0 12 722.0 13 1087.0 14 1639.0 15 2161.0 16 2566.0 17 3324.0 18 4009.0 19 4744.0 20 5680.0 21 6573.0 22 7513.0 23 8010.0 24 8301.0 25 8731.0 26 10006.0 27 11271.0 28 12994.0 29 14959.0 30 17428.0 31 21104.0 32 26217.0 33 33496.0 34 46351.0 35 68737.0 36 115888.0 37 208034.0 38 252151.0 39 79305.0 40 95.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78685298008918 18.920177057641734 13.588643156983487 23.704326805285593 2 15.179056758672385 19.177506616151057 41.272340664215676 24.371095960960883 3 18.911233132788343 24.197862459871487 29.4105728513873 27.48033155595287 4 12.409662359248456 16.359769908908696 38.07400557302785 33.156562158815 5 13.626204592221338 37.56219057514822 35.079660982417536 13.731943850212906 6 32.24115601098015 36.86037371606376 17.44391815390129 13.454552119054794 7 28.292737555294252 31.86760940498552 21.517340378418826 18.322312661301407 8 25.676762534996183 36.864240264698395 19.409213540341053 18.049783659964366 9 25.656826444295355 16.298315824409197 20.13762126359491 37.90723646770054 10-11 25.05677736704729 26.451456560286847 28.20362570771251 20.288140364953357 12-13 25.402954046135086 23.456903871331622 28.398435829720864 22.74170625281243 14-15 22.44776921780716 25.406974761124896 25.690891106347213 26.454364914720735 16-17 22.55390122854121 28.510902286225694 26.362378976486863 22.572817508746237 18-19 22.534793940182478 27.90020654772554 27.745905638590273 21.819093873501714 20-21 23.27369288973266 26.957229881828297 27.70519511462822 22.063882113810813 22-23 22.970038792691774 26.860078073086324 27.49683501218241 22.67304812203949 24-25 22.831348315291716 26.991198996957955 27.4923644033871 22.685088284363232 26-27 22.72608579911116 27.05333029873827 27.57253054746669 22.648053354683878 28-29 22.694994747034666 27.155138253576993 27.53371753474374 22.616149464644597 30-31 22.955966535461073 26.953503636433833 27.53366673237107 22.556863095734023 32-33 22.730454803161127 27.241451484750144 27.499324328443436 22.528769383645294 34-35 22.982231362133536 27.074108469161946 27.452636947956016 22.491023220748502 36-37 22.988632461090464 27.018429068711225 27.348695293464985 22.644243176733326 38-39 22.86264257685891 27.20131761033767 27.449182386614186 22.486857426189232 40-41 22.954239254790156 27.085742212504293 27.38349491874669 22.576523613958866 42-43 22.914969020713144 27.025643005630933 27.469554138056463 22.589833835599457 44-45 22.905108649809957 27.122388937473897 27.260767175054067 22.71173523766208 46-47 22.90276054582675 27.112741846225436 27.138044601069232 22.84645300687858 48-49 22.935825532499095 27.104598127799783 27.254191094591363 22.705385245109756 50-51 22.934494948519326 27.286230915244726 27.080755571777594 22.698518564458357 52-53 22.899657698081878 27.392287759338313 27.011754585844166 22.69629995673564 54-55 22.91784454812061 27.186741156228774 27.105848664434962 22.789565631215655 56-57 22.96722774769918 27.301343491960157 26.985954749013935 22.74547401132673 58-59 22.93580034344637 27.16867264638296 27.101651278363946 22.79387573180672 60-61 23.037158759068387 27.29352570690164 27.011986040028084 22.657329494001893 62-63 22.782967749295178 27.313334175317372 27.17236975050112 22.73132832488633 64-65 22.90751543003415 27.192672329814343 27.121709179274283 22.77810306087723 66-67 22.815271309235268 27.257808105763253 27.01507459999573 22.91184598500575 68-69 22.80551321622758 27.144823896367566 27.097983630827503 22.951679256577346 70-71 22.80778829315321 26.978446531248157 27.152651928869687 23.061113246728944 72-73 22.76101400476773 27.167480184018792 26.989846835721693 23.081658975491784 74-75 22.75049043948262 27.30343755341924 27.015228044184326 22.930843962913823 76-77 22.71372462763092 27.209393262683257 27.049824698606855 23.027057411078967 78-79 22.816241040326858 27.15030883495378 26.996819821602724 23.036630303116638 80-81 22.817618402848574 27.123019373694223 26.944246287890767 23.11511593556644 82-83 22.838481682048155 27.125568860753056 26.96717768794651 23.068771769252283 84-85 22.73532847405756 27.152919710070783 27.07262113566289 23.039130680208768 86-87 22.711798745054494 27.2062973006775 27.003088048530255 23.078815905737752 88-89 22.809477171046545 27.01223598953264 27.001404530092643 23.176882309328175 90-91 22.848718569501607 26.954087414372935 27.04152009781881 23.155673918306643 92-93 22.82508191707585 26.966098874508486 27.061972004435404 23.14684720398026 94-95 22.698449600178254 27.047118378675634 27.050526745249016 23.2039052758971 96-97 22.716458300388123 26.957123513143422 27.146226906784076 23.180191279684376 98-99 22.696157236944458 26.860548710486633 26.992433648250568 23.45086040431834 100-101 22.72295868076966 26.7902703763369 27.082571866769488 23.40419907612395 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 15.0 1 10.5 2 11.5 3 21.0 4 29.0 5 36.0 6 43.5 7 81.5 8 137.5 9 179.0 10 210.5 11 216.5 12 225.5 13 247.5 14 287.0 15 304.0 16 320.5 17 396.0 18 461.5 19 519.5 20 566.5 21 700.0 22 925.0 23 1133.0 24 1484.0 25 2060.5 26 3017.5 27 4475.0 28 6498.5 29 9078.0 30 11783.5 31 14387.0 32 16938.0 33 19411.5 34 21601.0 35 22832.0 36 23797.5 37 25259.0 38 26023.5 39 26947.5 40 28455.0 41 29859.5 42 31425.5 43 32960.0 44 34824.0 45 36651.0 46 37835.5 47 38939.5 48 39839.0 49 40242.5 50 39546.0 51 38570.5 52 37538.0 53 36379.0 54 35074.0 55 32734.5 56 30015.0 57 26607.0 58 23250.0 59 20342.0 60 17116.0 61 13897.0 62 10815.5 63 8068.5 64 5900.0 65 4200.0 66 2938.0 67 2094.0 68 1520.0 69 1056.5 70 772.5 71 594.5 72 413.0 73 264.0 74 196.5 75 146.5 76 103.0 77 84.5 78 61.5 79 45.0 80 34.0 81 18.5 82 21.5 83 18.0 84 12.5 85 12.5 86 9.0 87 8.0 88 7.5 89 5.0 90 4.0 91 6.0 92 3.5 93 0.5 94 1.5 95 3.0 96 2.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.471242808924148 2 0.3709589252656456 3 0.20818812321810676 4 0.2371454756422944 5 0.2586856816560761 6 0.21032182287041534 7 0.19924690562748043 8 0.19873888190074027 9 0.197621229701912 10-11 0.2113886726965696 12-13 0.19914530088213242 14-15 0.20834053033612884 16-17 0.09408599419227276 18-19 0.042064364574083066 20-21 2.5401186337006685E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.14148460789712722 46-47 0.21331916285818212 48-49 0.08849773319813128 50-51 0.06431580380530093 52-53 0.07290140478720919 54-55 0.08148700576911744 56-57 0.1151689788519883 58-59 0.09525444876377506 60-61 0.14184022450584532 62-63 0.2436481793445681 64-65 0.1322385760704568 66-67 0.10455128296311951 68-69 0.10947911311249882 70-71 0.08981859488765563 72-73 0.07310461427790522 74-75 0.1436691099221098 76-77 0.22180315909474235 78-79 0.1421958411145634 80-81 0.08499236948362436 82-83 0.08951378065161156 84-85 0.10165554772070076 86-87 0.0748826973214957 88-89 0.09743895078875764 90-91 0.11826792358510313 92-93 0.16840986541435432 94-95 0.13518511368554958 96-97 0.08956458302428556 98-99 0.07854046815402466 100-101 0.06426500143262691 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 984206.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.30075655046208 #Duplication Level Percentage of deduplicated Percentage of total 1 77.36646938258941 52.84188390469018 2 13.692327966508449 18.703927180991542 3 4.466408181094837 9.151771734958519 4 1.9088525669277288 5.215042978578217 5 0.9541456263204952 3.258443406850215 6 0.5227248548901748 2.1421501834037673 7 0.3170525962292212 1.5158452532120794 8 0.19498965504192214 1.0654352767101525 9 0.13769947343809505 0.8464480391179936 >10 0.4293023938889023 4.520882176689021 >50 0.008232715453770823 0.3685142256039073 >100 0.0014954898805754563 0.18457250799790492 >500 2.990977363805087E-4 0.18508313119651384 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.0160474534802673E-4 1.0160474534802673E-4 10-11 0.0 0.0 0.0 1.0160474534802673E-4 1.0160474534802673E-4 12-13 0.0 0.0 0.0 2.0320949069605346E-4 4.06418981392107E-4 14-15 0.0 0.0 0.0 2.0320949069605346E-4 5.080237267401337E-4 16-17 0.0 0.0 0.0 2.5401186337006685E-4 6.604308447621738E-4 18-19 0.0 0.0 0.0 3.048142360440802E-4 7.112332174361871E-4 20-21 0.0 0.0 0.0 3.5561660871809357E-4 7.112332174361871E-4 22-23 0.0 0.0 0.0 7.620355901102005E-4 7.112332174361871E-4 24-25 0.0 0.0 0.0 0.0011176521988282941 7.112332174361871E-4 26-27 0.0 0.0 0.0 0.001371664062198361 7.112332174361871E-4 28-29 0.0 0.0 0.0 0.0022353043976565882 7.112332174361871E-4 30-31 0.0 0.0 0.0 0.0044198064226391625 7.112332174361871E-4 32-33 0.0 0.0 0.0 0.010160474534802674 7.112332174361871E-4 34-35 0.0 0.0 0.0 0.019508111106821133 7.112332174361871E-4 36-37 0.0 0.0 0.0 0.03225950664799849 7.112332174361871E-4 38-39 0.0 0.0 0.0 0.05476495774258641 7.620355901102005E-4 40-41 0.0 0.0 0.0 0.08179182000516153 8.128379627842139E-4 42-43 0.0 0.0 0.0 0.11008874158458697 8.128379627842139E-4 44-45 0.0 0.0 0.0 0.14189102687851934 8.636403354582272E-4 46-47 0.0 0.0 0.0 0.17963718977531126 9.144427081322406E-4 48-49 0.0 0.0 0.0 0.2250037085732052 0.0010160474534802674 50-51 0.0 0.0 0.0 0.2705734368617952 0.0010160474534802674 52-53 0.0 0.0 0.0 0.31766723633060556 0.001371664062198361 54-55 0.0 0.0 0.0 0.3673519568057907 0.0014224664348723743 56-57 0.0 0.0 0.0 0.42668912808903825 0.0014224664348723743 58-59 0.0 0.0 0.0 0.4962375762797626 0.0014732688075463876 60-61 0.0 0.0 0.0 0.5708662617378882 0.001524071180220401 62-63 0.0 0.0 0.0 0.6480858682023884 0.0017272806709164544 64-65 0.0 0.0 0.0 0.7270835577104793 0.0017272806709164544 66-67 0.0 0.0 0.0 0.8194422712318357 0.0017272806709164544 68-69 0.0 0.0 0.0 0.9374561829535686 0.0017272806709164544 70-71 0.0 0.0 0.0 1.0592802726258528 0.0017272806709164544 72-73 0.0 0.0 0.0 1.1910108249695694 0.0018796877889384945 74-75 0.0 0.0 0.0 1.3235034129033962 0.0019812925342865212 76-77 0.0 0.0 0.0 1.4609238309866024 0.002032094906960535 78-79 0.0 0.0 0.0 1.6318738150346572 0.0021336996523085615 80-81 0.0 0.0 0.0 1.827920171183675 0.0021336996523085615 82-83 0.0 0.0 0.0 2.02874195036405 0.0022353043976565882 84-85 0.0 0.0 0.0 2.2247883065130676 0.0022353043976565882 86-87 0.0 0.0 0.0 2.4350085246381346 0.002286106770330602 88-89 0.0 0.0 0.0 2.680383984653619 0.002336909143004615 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1345 0.0 37.193222 1 GTATCAA 2020 0.0 31.840448 1 TCAACGC 2245 0.0 27.537622 4 ACTGCGC 140 5.7766556E-7 27.157885 8 ATCAACG 2290 0.0 26.99649 3 CAACGCA 2315 0.0 26.704952 5 GTACATG 2725 0.0 26.575035 1 TACATGG 2630 0.0 25.883472 2 AACGCAG 2415 0.0 25.78031 6 TAACGCT 95 0.0012181281 25.029135 4 ACATGGG 2635 0.0 24.725176 3 TATCAAC 2600 0.0 24.351208 2 TACCTGG 865 0.0 24.214684 2 GTACCTG 980 0.0 22.84907 1 ACGCAGA 2720 0.0 22.713617 7 TATAGGG 210 3.36604E-8 22.668507 2 TACACCG 105 0.0021811803 22.645409 5 CATGGGG 1825 0.0 21.627916 4 CGCAGAG 2845 0.0 21.215607 8 GTACACG 180 5.034901E-6 21.174555 1 >>END_MODULE