FastQCFastQC Report
Fri 10 Feb 2017
SRR2031591_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031591_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences984206
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13000.041.6874661
GTATCAA20500.033.624681
GCACCGT1201.506869E-731.6632466
TCAACGC22500.030.190234
ATCAACG22450.030.0458763
CAACGCA23100.029.6071915
AACGCAG24300.028.5360136
TATCAAC24850.028.099842
CGCAGAG27550.024.99738
ACGCAGA27950.024.8094847
GTACATG27250.022.6788431
TACATGG27450.021.4581472
GCAGAGT31200.021.31189
ACATGGG27250.021.092683
AGGACCG2056.8342706E-720.8514065
GTCGTAG1150.003705539720.6688421
GTTCGCA1150.00372523520.6499429
GAGTACT15750.020.0533912-13
GTATAAG4350.019.6710361
CCAATAC3707.2759576E-1219.2576123
Produced by FastQC (version 0.11.5)