##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031591_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 984206 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.455298992284135 34.0 31.0 34.0 31.0 34.0 2 32.694223567017474 34.0 31.0 34.0 31.0 34.0 3 32.80725376597989 34.0 31.0 34.0 31.0 34.0 4 36.25547293960817 37.0 37.0 37.0 35.0 37.0 5 35.89726845802606 37.0 35.0 37.0 35.0 37.0 6 35.95656193926881 37.0 36.0 37.0 35.0 37.0 7 35.926536720971015 37.0 36.0 37.0 35.0 37.0 8 36.052569279195616 37.0 36.0 37.0 35.0 37.0 9 37.96864883977541 39.0 39.0 39.0 35.0 39.0 10-11 37.94661483469924 39.0 38.0 39.0 35.0 39.0 12-13 37.96304991028301 39.0 38.0 39.0 35.0 39.0 14-15 39.4438593140054 41.0 39.0 41.0 36.0 41.0 16-17 39.40223896216849 41.0 39.0 41.0 36.0 41.0 18-19 39.39430667969917 41.0 39.0 41.0 36.0 41.0 20-21 39.410320095589746 41.0 39.0 41.0 36.0 41.0 22-23 39.346210041393775 41.0 39.0 41.0 36.0 41.0 24-25 39.28130543808918 41.0 39.0 41.0 36.0 41.0 26-27 39.17949595917928 41.0 39.0 41.0 36.0 41.0 28-29 39.09028343659762 40.5 39.0 41.0 36.0 41.0 30-31 38.99668565320675 40.0 39.0 41.0 35.0 41.0 32-33 38.85720265879297 40.0 38.0 41.0 35.0 41.0 34-35 38.75697719786305 40.0 38.0 41.0 35.0 41.0 36-37 38.626318067559026 40.0 38.0 41.0 35.0 41.0 38-39 38.51935824410744 40.0 38.0 41.0 34.0 41.0 40-41 38.4080736146701 40.0 38.0 41.0 34.0 41.0 42-43 38.26100226985001 40.0 38.0 41.0 34.0 41.0 44-45 38.203664171931486 40.0 38.0 41.0 33.5 41.0 46-47 38.21793151027326 40.0 38.0 41.0 33.5 41.0 48-49 38.33836767912408 40.0 38.0 41.0 34.0 41.0 50-51 38.25951934859165 40.0 38.0 41.0 34.0 41.0 52-53 38.16067418812728 40.0 38.0 41.0 33.5 41.0 54-55 38.03905178387451 40.0 37.5 41.0 33.0 41.0 56-57 37.91048113911113 40.0 37.0 41.0 33.0 41.0 58-59 37.70175603481385 40.0 37.0 41.0 33.0 41.0 60-61 37.400467483433346 39.5 36.0 41.0 32.0 41.0 62-63 37.199704635005276 39.0 36.0 41.0 32.0 41.0 64-65 36.88501492573709 39.0 35.0 41.0 32.0 41.0 66-67 36.57600492173387 38.0 35.0 40.5 31.0 41.0 68-69 36.26315019416667 37.0 35.0 40.0 31.0 41.0 70-71 35.91028656602378 37.0 35.0 39.5 31.0 41.0 72-73 35.52383596523492 36.0 35.0 39.0 31.0 41.0 74-75 35.152385781025515 36.0 34.5 39.0 30.0 40.5 76-77 34.54827089044367 35.0 34.0 37.0 29.5 39.0 78-79 34.50341341141997 35.0 34.0 37.0 30.0 39.0 80-81 34.245111287677574 35.0 34.0 37.0 30.0 39.0 82-83 33.97447892006348 35.0 34.0 36.0 30.0 38.0 84-85 33.72615590638545 35.0 34.0 36.0 29.5 37.0 86-87 33.49214290504224 35.0 34.0 35.5 29.0 37.0 88-89 33.27808761580401 35.0 34.0 35.0 29.0 36.5 90-91 33.12857318488203 35.0 34.0 35.0 29.0 36.0 92-93 33.00724594241449 35.0 34.0 35.0 29.0 36.0 94-95 32.90222930971768 35.0 34.0 35.0 29.0 36.0 96-97 32.805767288555444 35.0 34.0 35.0 29.0 35.5 98-99 32.71332119495309 35.0 33.0 35.0 28.5 35.0 100-101 32.28391922016326 34.5 32.5 35.0 26.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2271539196857475 1101 2 0.18650846147814093 1101 3 0.2102070805212577 1101 4 0.14655317455063255 1101 5 0.15778723911039094 1101 6 0.024134954808346265 1101 7 0.16860477850448063 1101 8 0.23138581969573124 1101 9 0.25401784235550906 1101 10-11 0.14073282507424523 1101 12-13 0.11389974627095256 1101 14-15 0.1357646465837945 1101 16-17 0.2125180812162526 1101 18-19 0.1669085715782046 1101 20-21 0.05361332160413923 1101 22-23 0.20590145343650335 1101 24-25 -0.021927398536817577 1101 26-27 0.2184806359634237 1101 28-29 0.23281160308191318 1101 30-31 0.03715627777865649 1101 32-33 0.11202637078741873 1101 34-35 -0.02803391032529845 1101 36-37 0.04494661608781314 1101 38-39 0.14799225120584225 1101 40-41 -0.035330779587170014 1101 42-43 -0.02247753027434385 1101 44-45 0.08828526383798874 1101 46-47 0.11980918846358435 1101 48-49 0.05559997530349392 1101 50-51 0.11769266249833521 1101 52-53 0.12355203617663335 1101 54-55 0.26221458094552474 1101 56-57 0.4959782923510616 1101 58-59 0.20038559357197272 1101 60-61 0.08891549366884988 1101 62-63 0.08593155355104187 1101 64-65 0.16841848489939082 1101 66-67 0.209370139744955 1101 68-69 0.1098127547528307 1101 70-71 0.18044892875926166 1101 72-73 0.0738393029628881 1101 74-75 0.02596418023300373 1101 76-77 -0.04364492078681792 1101 78-79 0.17419571485954322 1101 80-81 0.27663099647645595 1101 82-83 0.28459826096561613 1101 84-85 0.23123144142063268 1101 86-87 0.04025723813848714 1101 88-89 0.13238757311491156 1101 90-91 0.12369424722995603 1101 92-93 -0.07828698269445056 1101 94-95 0.1810268324758013 1101 96-97 0.13315823585035957 1101 98-99 -0.23446025362256506 1101 100-101 -0.16020898985885168 1102 1 0.06548848277744668 1102 2 0.18558837283359253 1102 3 0.16656531359424065 1102 4 0.15740308413472093 1102 5 -0.0034767740817969184 1102 6 0.056423439554883714 1102 7 0.06946713859557008 1102 8 0.02677951441236104 1102 9 0.231369898600704 1102 10-11 0.2052333329071061 1102 12-13 0.080436937859659 1102 14-15 0.28423407530049616 1102 16-17 0.21764419835296422 1102 18-19 0.20466725093240257 1102 20-21 0.19312654118800054 1102 22-23 0.15026603792472315 1102 24-25 0.22843993602244694 1102 26-27 0.3013344909691753 1102 28-29 -0.13522321755814204 1102 30-31 -0.11471633199798958 1102 32-33 0.15442372419448702 1102 34-35 -0.0162843018727159 1102 36-37 0.07665590375825104 1102 38-39 0.06186966022603002 1102 40-41 0.19236630989973236 1102 42-43 0.2545207633562754 1102 44-45 0.012912545103475281 1102 46-47 -0.010854868311746202 1102 48-49 -0.07286580874074389 1102 50-51 0.13235354866348814 1102 52-53 0.16914803570256964 1102 54-55 -9.619932407645138E-4 1102 56-57 -0.022318345757177838 1102 58-59 0.05690719972561453 1102 60-61 0.14191999088883733 1102 62-63 0.1085750370423284 1102 64-65 0.0607031210756972 1102 66-67 0.26245810761066224 1102 68-69 0.014514127367071694 1102 70-71 0.20063353269423345 1102 72-73 0.2214638303342511 1102 74-75 0.21091155549536467 1102 76-77 0.18981865224107963 1102 78-79 0.19403879004557467 1102 80-81 0.02076670572844108 1102 82-83 -0.13225478099781895 1102 84-85 -0.12731257564561105 1102 86-87 -0.12397285028942662 1102 88-89 -0.15905432812975562 1102 90-91 0.1268999023343511 1102 92-93 0.11229497974503744 1102 94-95 0.10742738707771338 1102 96-97 0.2004049916458399 1102 98-99 0.14876240145760988 1102 100-101 0.09581268447309554 1103 1 0.11685107712061438 1103 2 0.15847321966106165 1103 3 0.10648871809068794 1103 4 0.15374822953437217 1103 5 0.10070937097663801 1103 6 0.06655774653064128 1103 7 0.1818339002367182 1103 8 0.2008033159292424 1103 9 0.19233058212133614 1103 10-11 0.20990506734317194 1103 12-13 0.07576889892874306 1103 14-15 0.1802924494999658 1103 16-17 0.20050690910454705 1103 18-19 0.21417948120672747 1103 20-21 0.15537387642082479 1103 22-23 0.057495924214030936 1103 24-25 0.1251788990883398 1103 26-27 0.14060034191466997 1103 28-29 0.19950455305446013 1103 30-31 0.23478668415013004 1103 32-33 0.043961995461813785 1103 34-35 -0.03891865504228775 1103 36-37 0.00706153472263793 1103 38-39 0.1891798128873461 1103 40-41 0.0072104150146827806 1103 42-43 -0.13845987096487278 1103 44-45 -0.039858652622058344 1103 46-47 -0.055898764753834485 1103 48-49 -0.17436593746750617 1103 50-51 -0.011432397823135432 1103 52-53 -0.10836592406815981 1103 54-55 -0.03280123383908773 1103 56-57 -0.004730204879585642 1103 58-59 0.07783669318272501 1103 60-61 -0.05415550581785311 1103 62-63 -0.09788667690832398 1103 64-65 0.08011745741840315 1103 66-67 0.08289474386332785 1103 68-69 0.05548311345595636 1103 70-71 0.12181518278656256 1103 72-73 0.06366945563220128 1103 74-75 0.13540684187853458 1103 76-77 0.20776279344879356 1103 78-79 0.14499820477610115 1103 80-81 0.06766143433669924 1103 82-83 0.06685472544283044 1103 84-85 -0.04718108957212053 1103 86-87 -0.13559151850327567 1103 88-89 -0.1228359139202837 1103 90-91 0.1281711561083796 1103 92-93 -0.057745683130036696 1103 94-95 -0.003551096459425196 1103 96-97 0.0014993539063539174 1103 98-99 0.06710036033157252 1103 100-101 -0.10208469627603733 1104 1 0.14990822043228036 1104 2 0.17410848730351347 1104 3 0.036766951698247397 1104 4 0.06876564896263204 1104 5 0.08948800412360214 1104 6 0.15195026798523514 1104 7 0.0254334993512515 1104 8 0.13715234571542112 1104 9 0.13306903861437291 1104 10-11 0.1325712494230089 1104 12-13 0.08488671838588857 1104 14-15 0.08997145967016706 1104 16-17 0.16825985792556963 1104 18-19 0.11024784221412176 1104 20-21 2.3266708298308458E-4 1104 22-23 0.05274183367705376 1104 24-25 -0.032013850219968276 1104 26-27 0.01035539305938471 1104 28-29 -0.12189032280688394 1104 30-31 -0.07682284165228026 1104 32-33 -0.11817513037536287 1104 34-35 0.17378437276883574 1104 36-37 0.3050365491317564 1104 38-39 0.1188798462944689 1104 40-41 0.014899559902481485 1104 42-43 -0.07255912071186543 1104 44-45 0.15252966296144166 1104 46-47 0.2196090418666472 1104 48-49 0.12473916770374416 1104 50-51 0.17899309633918392 1104 52-53 0.22715416854176596 1104 54-55 0.06552339283937414 1104 56-57 0.11605612291892697 1104 58-59 0.2687076156476067 1104 60-61 0.3472641133409482 1104 62-63 0.21244040696802102 1104 64-65 -0.009627167767142453 1104 66-67 0.05579275669018813 1104 68-69 0.06402273271265813 1104 70-71 0.10276394507101827 1104 72-73 0.12221819148464874 1104 74-75 -0.07504212292895573 1104 76-77 0.10214244275967133 1104 78-79 0.1149752358496201 1104 80-81 0.0018035137281771085 1104 82-83 0.11169552765431234 1104 84-85 0.1776298787327235 1104 86-87 0.10338443693431287 1104 88-89 0.09909690227568291 1104 90-91 -0.12746124806641035 1104 92-93 -0.13682511498868877 1104 94-95 -0.006264048511035014 1104 96-97 -0.07877624873734135 1104 98-99 0.044385352457034344 1104 100-101 0.11189037197606666 1105 1 0.15287546811653385 1105 2 0.094217982261533 1105 3 0.02698910824226175 1105 4 0.08597451270614442 1105 5 0.11373616454731916 1105 6 0.028737946753111032 1105 7 0.1524575023912078 1105 8 0.13774462242709973 1105 9 0.05769508330839557 1105 10-11 0.07770238300545174 1105 12-13 0.1378409477107425 1105 14-15 0.1325878215195715 1105 16-17 0.08737362431286755 1105 18-19 0.08811545840005408 1105 20-21 0.04024477063875764 1105 22-23 0.037929051759199695 1105 24-25 0.12025990594030134 1105 26-27 0.0966436594126705 1105 28-29 0.030765774359345244 1105 30-31 -0.0010735822913758852 1105 32-33 0.0651855297844719 1105 34-35 0.27681002714664515 1105 36-37 0.2694295769206718 1105 38-39 0.07417937411505449 1105 40-41 0.06361637188218339 1105 42-43 0.08358144003930335 1105 44-45 -0.0774537298341258 1105 46-47 0.039466613977801046 1105 48-49 0.008744412577911476 1105 50-51 0.2488846803803142 1105 52-53 0.3000053769035418 1105 54-55 0.053094964495471686 1105 56-57 0.023680893924812096 1105 58-59 0.12992252178640484 1105 60-61 0.20748915503487808 1105 62-63 0.16383032207894388 1105 64-65 0.08938859734881532 1105 66-67 0.12252240406857595 1105 68-69 0.1268304527170656 1105 70-71 0.3108465870239172 1105 72-73 0.2950245979248649 1105 74-75 0.37276011998190484 1105 76-77 0.227768438195028 1105 78-79 0.11942845302634453 1105 80-81 0.21731717596860278 1105 82-83 0.19810752706320756 1105 84-85 0.1624429655851145 1105 86-87 0.21813306531527843 1105 88-89 0.19179524460457742 1105 90-91 0.17913870830449952 1105 92-93 0.17248406752871404 1105 94-95 0.06636608230589047 1105 96-97 0.12832767060982064 1105 98-99 -0.12882391942964233 1105 100-101 -0.24045035250150448 1106 1 0.16783593913007167 1106 2 0.15626225823616124 1106 3 0.1482040213311393 1106 4 0.16710326050156254 1106 5 0.17533732979691052 1106 6 0.12030173276134093 1106 7 0.25022264493247093 1106 8 0.19682312760842535 1106 9 0.2852916682847493 1106 10-11 0.31472603897604046 1106 12-13 0.17175975754165052 1106 14-15 0.22484698425966343 1106 16-17 0.17110180878731995 1106 18-19 0.23346016344625298 1106 20-21 0.1446017336192682 1106 22-23 0.09757131662535556 1106 24-25 0.044382678321781555 1106 26-27 0.17376247450827265 1106 28-29 0.18409305330850856 1106 30-31 0.14620918377248415 1106 32-33 0.13759026097859817 1106 34-35 0.041979988626721365 1106 36-37 0.052352484428986656 1106 38-39 -0.021251759458699837 1106 40-41 0.01654463181707655 1106 42-43 0.17311174267076268 1106 44-45 -0.03056735044360437 1106 46-47 -0.01223914295183448 1106 48-49 -0.0374608485941792 1106 50-51 0.19001456885681023 1106 52-53 0.14329115261054426 1106 54-55 0.22743819543074295 1106 56-57 0.2058169508535741 1106 58-59 0.10557556170146398 1106 60-61 0.16976441892705907 1106 62-63 0.15487816926277986 1106 64-65 0.15309234492486468 1106 66-67 0.19802781352920817 1106 68-69 0.13852462734656257 1106 70-71 0.1491111723863412 1106 72-73 0.29634625243889445 1106 74-75 0.26290046762639463 1106 76-77 0.35440378000450323 1106 78-79 0.41035477380341234 1106 80-81 0.3175962041083267 1106 82-83 0.3318754160135171 1106 84-85 0.19852687583983908 1106 86-87 0.17662324229633697 1106 88-89 0.3700957614208562 1106 90-91 0.38028026682582095 1106 92-93 0.21730844651561654 1106 94-95 0.1388333315973469 1106 96-97 0.030475880179253068 1106 98-99 0.26773824330167173 1106 100-101 0.5130638226544662 1107 1 0.030817984795810105 1107 2 0.12145291932736058 1107 3 0.09115918527644595 1107 4 0.10685584911167467 1107 5 0.09278389822914335 1107 6 0.14063310804343132 1107 7 0.09427133071503135 1107 8 0.08327689768675128 1107 9 0.08509337411243934 1107 10-11 0.13610779259345662 1107 12-13 0.03078836500216653 1107 14-15 0.11336044171353166 1107 16-17 0.17257656983870362 1107 18-19 -0.006291180639777849 1107 20-21 0.09487262503039773 1107 22-23 0.13190151375796688 1107 24-25 0.10470476115730776 1107 26-27 -0.03132679935340832 1107 28-29 -0.03212434016023025 1107 30-31 -0.002212759676339715 1107 32-33 0.058822256863365396 1107 34-35 0.019879314924345692 1107 36-37 0.0657726025758052 1107 38-39 0.06685639798190124 1107 40-41 0.007766588540825126 1107 42-43 -0.14233570522515748 1107 44-45 -0.1877486693072683 1107 46-47 0.1419947280159093 1107 48-49 0.13797763872826607 1107 50-51 -0.028065462339263547 1107 52-53 -0.07904515098695697 1107 54-55 0.10400007316869875 1107 56-57 0.03734779845745351 1107 58-59 -0.12280290637234259 1107 60-61 0.1851902950720259 1107 62-63 -0.04308620416082931 1107 64-65 0.024447963536310624 1107 66-67 0.041703142083569844 1107 68-69 0.017924020018803333 1107 70-71 0.11818267444395758 1107 72-73 0.19381406074899843 1107 74-75 0.06870077958710397 1107 76-77 -0.05078837451560503 1107 78-79 -0.03056144483458212 1107 80-81 -0.014287258655507173 1107 82-83 -0.08221715998542578 1107 84-85 0.08894238095068374 1107 86-87 0.02159702432208377 1107 88-89 -0.1338203410615506 1107 90-91 0.13503642307167496 1107 92-93 0.22611138027374267 1107 94-95 0.1995391426951869 1107 96-97 0.09547529940134325 1107 98-99 0.2074472735684978 1107 100-101 0.2113538465493292 1108 1 -0.05109899624578418 1108 2 -0.05923040654622724 1108 3 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64-65 -0.8240212851148172 2316 66-67 -0.8895726873275223 2316 68-69 -0.9962014391707328 2316 70-71 -1.0142127881894965 2316 72-73 -1.2097130888319896 2316 74-75 -0.8140659808307618 2316 76-77 -0.7946395397090384 2316 78-79 -0.7943209873670085 2316 80-81 -0.9961367162378778 2316 82-83 -1.4897675990854111 2316 84-85 -1.4478071311604381 2316 86-87 -1.0162358880875146 2316 88-89 -0.7809044875430189 2316 90-91 -0.5480492588247827 2316 92-93 -0.7133258255579662 2316 94-95 -0.7401940251385852 2316 96-97 -0.7223322858357051 2316 98-99 -0.6360390511933005 2316 100-101 -0.8991709209859735 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 3.0 13 4.0 14 39.0 15 111.0 16 234.0 17 507.0 18 830.0 19 1371.0 20 2000.0 21 2755.0 22 3438.0 23 3676.0 24 3806.0 25 4337.0 26 5021.0 27 6408.0 28 8168.0 29 10350.0 30 13272.0 31 16609.0 32 21303.0 33 28303.0 34 39778.0 35 60032.0 36 109224.0 37 228064.0 38 313136.0 39 101285.0 40 142.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.25459269560126 19.132572153504597 13.984747940504363 23.62808721038978 2 15.51546852646638 19.404953505007153 40.799457829366624 24.280120139159838 3 19.08429367142111 24.068534711370226 29.744503668962263 27.102667948246395 4 12.546565673604352 16.54262702546708 37.963548197631525 32.94725910329704 5 13.376671144049112 37.449781854611736 35.51410985098648 13.659437150352671 6 32.11177334826246 36.93556023840537 17.591032771594563 13.361633641737603 7 28.276803839846536 31.752397363966487 21.61691759651943 18.353881199667548 8 25.384015135042866 36.96461919557491 19.531683407741877 18.119682261640346 9 25.37507391745224 16.2735240386667 20.19414634741101 38.15725569647005 10-11 24.891994155695045 26.435217830413553 28.410464882351867 20.262323131539535 12-13 25.25970172910956 23.372545991387984 28.59655397345678 22.771198306045687 14-15 22.321851319744038 25.318073655311995 25.82594497493411 26.534130050009857 16-17 22.40923140074334 28.504449271798787 26.53341881679242 22.55290051066545 18-19 22.348065344043828 27.747951140310057 28.06836170476506 21.835621810881058 20-21 22.97837038181031 26.876639646578056 28.025179687992146 22.119810283619486 22-23 22.804570068782695 26.755396461951065 27.725588595251 22.714444874015243 24-25 22.532427154477823 26.909915200679535 27.855093344279553 22.702564300563093 26-27 22.584499586468688 26.97773636819934 27.779601018485966 22.658163026846008 28-29 22.49294070599546 27.059111075202757 27.839507960729136 22.608440258072644 30-31 22.74824178844959 26.857586515796594 27.842063035968074 22.55210865978575 32-33 22.61137404161324 27.03062163815299 27.722245139737005 22.635759180496766 34-35 22.728625917744864 26.985813945454506 27.75165971351526 22.53390042328537 36-37 22.710387865954893 26.925714738581153 27.65076620138467 22.71313119407929 38-39 22.644344781478672 27.10306582158613 27.714116760109164 22.53847263682603 40-41 22.803356207948337 26.96224164453377 27.636897153644664 22.597504993873233 42-43 22.729235546216948 26.920329687077704 27.703092645238904 22.64734212146644 44-45 22.855631849429898 27.01243438873569 27.483423185796468 22.648510576037946 46-47 22.742444163112193 27.013856855170566 27.369575068634006 22.874123913083235 48-49 22.729356329526272 26.982598363813214 27.504667777243608 22.783377529416907 50-51 22.64041275978488 27.212321824597414 27.38201873270959 22.765246682908117 52-53 22.777904219238657 27.238504947135052 27.317756648506514 22.66583418511978 54-55 22.786201739185213 27.017724610888173 27.347028769153315 22.8490448807733 56-57 22.785524575139757 27.19760903713247 27.223213432960176 22.793652954767598 58-59 22.78339087548745 27.054092334328384 27.319179114941385 22.843337675242783 60-61 22.821391050247612 27.213103760798045 27.26146761958371 22.704037569370637 62-63 22.592424756605833 27.18851541242382 27.428455018563184 22.79060481240716 64-65 22.72059914286237 27.018530673456574 27.4458294300177 22.81504075366336 66-67 22.656791362783807 27.231849836314755 27.13593495670622 22.97542384419522 68-69 22.66949195595231 27.04184896251395 27.28051850933646 23.008140572197284 70-71 22.606344606718512 26.97951445124293 27.374960120137448 23.039180821901105 72-73 22.69306425687305 27.025287389022218 27.183536779901768 23.09811157420296 74-75 22.577438056667 27.19532293036214 27.190191890722065 23.037047122248797 76-77 22.617368721588775 27.111346608331992 27.13263280248241 23.138651867596824 78-79 22.686693033191847 27.054188543516194 27.148376477401754 23.1107419458902 80-81 22.65898373771005 27.104934373147078 27.056361407254343 23.179720481888534 82-83 22.591304400840112 27.166863282436776 27.107624525861322 23.134207790861787 84-85 22.57926694208326 27.106367975809942 27.193951266299944 23.120413815806852 86-87 22.505755908823964 27.218539614674164 27.15305535629736 23.12264912020451 88-89 22.63845170624849 26.958329861837864 27.048656480452266 23.35456195146138 90-91 22.657909014982636 26.923631841301514 27.129686264867313 23.288772878848533 92-93 22.69977017006602 27.013044017207783 27.09087325214437 23.19631256058183 94-95 22.562095740119446 26.998362131504987 27.067758172577694 23.371783955797873 96-97 22.514290707433197 26.96595021773897 27.15849121017348 23.36126786465435 98-99 22.599943507761587 26.850628831768958 26.92104092029514 23.628386740174314 100-101 22.500066043084477 26.879128962839083 27.037175144227938 23.58362984984851 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 13.0 1 8.0 2 14.5 3 25.0 4 18.0 5 16.0 6 29.5 7 68.5 8 128.0 9 175.5 10 183.5 11 170.5 12 190.0 13 204.5 14 221.5 15 276.5 16 314.5 17 357.0 18 410.5 19 447.5 20 522.5 21 647.5 22 782.5 23 986.5 24 1301.5 25 1868.0 26 2864.0 27 4276.5 28 6170.5 29 8819.0 30 11762.5 31 14391.5 32 16824.0 33 19340.0 34 21588.0 35 22634.5 36 23527.5 37 25083.0 38 26079.0 39 27206.0 40 28738.0 41 30260.0 42 31652.5 43 32917.5 44 35069.5 45 36933.0 46 38043.5 47 39429.0 48 40628.5 49 41132.0 50 40764.0 51 39820.5 52 38638.5 53 37446.5 54 36100.0 55 33920.0 56 31016.5 57 27334.0 58 23605.0 59 20341.5 60 16679.5 61 13088.5 62 9801.5 63 6853.0 64 4672.5 65 3090.0 66 2003.0 67 1323.0 68 878.5 69 596.0 70 445.0 71 311.5 72 221.5 73 167.5 74 113.0 75 78.5 76 55.5 77 37.0 78 22.5 79 10.0 80 9.0 81 7.0 82 3.5 83 3.5 84 1.5 85 0.5 86 1.0 87 0.5 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.047754230313572565 2 0.0014224664348723743 3 4.06418981392107E-4 4 0.011379731478978994 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0019812925342865217 24-25 0.0 26-27 0.0 28-29 0.022302241603891867 30-31 0.018339656535318827 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.033834380200892904 50-51 0.010008067416780633 52-53 0.0 54-55 9.652450808062539E-4 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.5561660871809357E-4 80-81 0.012141767069089196 82-83 0.0053850515034454165 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 984206.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.39896632510755 #Duplication Level Percentage of deduplicated Percentage of total 1 75.48784309094695 50.123147513507895 2 14.7504791698914 19.58833139361639 3 4.913627853197573 9.787794310757285 4 2.078039325202252 5.519186527054143 5 0.9942236517839169 3.3007711387212875 6 0.5724866245162895 2.2807512061698962 7 0.3406955966454616 1.5835284814142103 8 0.2042082916277432 1.0847375583278613 9 0.1534741768501074 0.9171474030399491 >10 0.49338427749191416 5.007602085498998 >50 0.00953947555405792 0.4110578008449723 >100 0.001537282051413023 0.14484289458840136 >500 4.6118424096062256E-4 0.25110168645876024 >1k 0.0 0.0 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences pass >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.0160474534802673E-4 2 0.0 0.0 0.0 0.0 1.0160474534802673E-4 3 0.0 0.0 0.0 0.0 1.0160474534802673E-4 4 0.0 0.0 0.0 0.0 1.0160474534802673E-4 5 0.0 0.0 0.0 0.0 1.0160474534802673E-4 6 0.0 0.0 0.0 0.0 1.0160474534802673E-4 7 0.0 0.0 0.0 0.0 1.0160474534802673E-4 8 0.0 0.0 0.0 0.0 3.048142360440802E-4 9 0.0 0.0 0.0 0.0 3.048142360440802E-4 10-11 0.0 0.0 0.0 0.0 3.048142360440802E-4 12-13 0.0 0.0 0.0 1.0160474534802673E-4 3.5561660871809357E-4 14-15 0.0 0.0 0.0 1.0160474534802673E-4 4.0641898139210693E-4 16-17 0.0 0.0 0.0 1.524071180220401E-4 4.0641898139210693E-4 18-19 0.0 0.0 0.0 2.0320949069605346E-4 4.0641898139210693E-4 20-21 0.0 0.0 0.0 2.5401186337006685E-4 4.0641898139210693E-4 22-23 0.0 0.0 0.0 6.604308447621738E-4 4.5722135406612034E-4 24-25 0.0 0.0 0.0 0.0010160474534802674 6.096284720881604E-4 26-27 0.0 0.0 0.0 0.0012700593168503342 6.096284720881604E-4 28-29 0.0 0.0 0.0 0.0021845020249825747 6.096284720881604E-4 30-31 0.0 0.0 0.0 0.004470608795313176 6.096284720881604E-4 32-33 0.0 0.0 0.0 0.010312881652824714 6.096284720881604E-4 34-35 0.0 0.0 0.0 0.01981292534286521 6.096284720881604E-4 36-37 0.0 0.0 0.0 0.032462716138694545 7.112332174361871E-4 38-39 0.0 0.0 0.0 0.05501896960595648 7.112332174361871E-4 40-41 0.0 0.0 0.0 0.08240144847724967 7.620355901102005E-4 42-43 0.0 0.0 0.0 0.11120639378341526 8.128379627842139E-4 44-45 0.0 0.0 0.0 0.1439231217854799 8.128379627842139E-4 46-47 0.0 0.0 0.0 0.18156767993692377 8.128379627842139E-4 48-49 0.0 0.0 0.0 0.22668018687144764 8.128379627842139E-4 50-51 0.0 0.0 0.0 0.2725039270234077 8.128379627842139E-4 52-53 0.0 0.0 0.0 0.31980093598291415 8.636403354582272E-4 54-55 0.0 0.0 0.0 0.3689776327313591 9.144427081322406E-4 56-57 0.0 0.0 0.0 0.4278575826605406 9.144427081322406E-4 58-59 0.0 0.0 0.0 0.4963899833977846 9.144427081322406E-4 60-61 0.0 0.0 0.0 0.5711710759739324 9.65245080806254E-4 62-63 0.0 0.0 0.0 0.6490511132831948 0.0010160474534802674 64-65 0.0 0.0 0.0 0.7280996051639597 0.0010668498261542808 66-67 0.0 0.0 0.0 0.8218807851201883 0.0011684545715023075 68-69 0.0 0.0 0.0 0.9402503134506394 0.0012192569441763208 70-71 0.0 0.0 0.0 1.0632936600670997 0.0013208616895243476 72-73 0.0 0.0 0.0 1.1962434693549928 0.0014224664348723743 74-75 0.0 0.0 0.0 1.3302093260963659 0.0014224664348723743 76-77 0.0 0.0 0.0 1.4698650485772287 0.0014224664348723743 78-79 0.0 0.0 0.0 1.6425931156688742 0.0014224664348723743 80-81 0.0 0.0 0.0 1.839401507408002 0.0014732688075463876 82-83 0.0 0.0 0.0 2.0429158123400994 0.0017272806709164544 84-85 0.0 0.0 0.0 2.24267074169432 0.0017780830435904678 86-87 0.0 0.0 0.0 2.455685090316458 0.0019812925342865212 88-89 0.0 0.0 0.0 2.7034482618476217 0.002032094906960535 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1300 0.0 41.687466 1 GTATCAA 2050 0.0 33.62468 1 GCACCGT 120 1.506869E-7 31.663246 6 TCAACGC 2250 0.0 30.19023 4 ATCAACG 2245 0.0 30.045876 3 CAACGCA 2310 0.0 29.607191 5 AACGCAG 2430 0.0 28.536013 6 TATCAAC 2485 0.0 28.09984 2 CGCAGAG 2755 0.0 24.9973 8 ACGCAGA 2795 0.0 24.809484 7 GTACATG 2725 0.0 22.678843 1 TACATGG 2745 0.0 21.458147 2 GCAGAGT 3120 0.0 21.3118 9 ACATGGG 2725 0.0 21.09268 3 AGGACCG 205 6.8342706E-7 20.851406 5 GTCGTAG 115 0.0037055397 20.668842 1 GTTCGCA 115 0.003725235 20.649942 9 GAGTACT 1575 0.0 20.05339 12-13 GTATAAG 435 0.0 19.671036 1 CCAATAC 370 7.2759576E-12 19.257612 3 >>END_MODULE