FastQCFastQC Report
Fri 10 Feb 2017
SRR2031590_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031590_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292755
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC43241.4770029546890746No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC20150.6882888422059401No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19790.6759918703352632No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19510.6664275588802924No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC19210.656180082321395No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA18120.6189475841574013No Hit
GTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAAAACATTTATCCT15000.5123738279448685No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG13330.4553295417670066No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA13070.4464483954159621No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT12620.43107718057761607No Hit
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC12110.41365647042749054No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC11970.4088743147000052No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT11820.40375057642055645No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC11630.3972605079332548No Hit
TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT11590.39589417772540175No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA11510.3931615173096958No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11470.39179518710184286No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11140.3805229628870557No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG11010.37608238971153357No Hit
GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT10860.37095865143208484No Hit
GTACTAGTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAAAACATT10240.3497805332103636No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA10190.3480726204505474No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT9930.339191474099503No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA9830.3357756485798705No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA9710.33167665795631157No Hit
ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA9580.3272360847807894No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC9330.31869652098170825No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8790.30025106317569294No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT8410.2872709262010897No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT8360.2855630134412734No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT8150.27838977985004526No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8130.2777066147461188No Hit
AGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTA7980.27258287646667007No Hit
GTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTT7490.25584533142047106No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA7440.2541374186606548No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7370.2517463407969121No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC7260.2479889327253164No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG7230.24696418506942666No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC7130.24354835954979417No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC7070.24149886423801473No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA6930.23671670851052928No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC6570.22441973663985243No Hit
GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC6570.22441973663985243No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC6510.22237024132807295No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA6480.2213454936721832No Hit
ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC6430.219637580912367No Hit
ACATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAG6210.2121227647691756No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC6030.20597427883383718No Hit
AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG5950.2032416184181312No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC5910.20187528821027823No Hit
GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT5900.201533705658315No Hit
TTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTT5890.2011921231063517No Hit
AGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAAT5840.1994842103465355No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT5690.19436047206708681No Hit
GTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTAT5630.19231097675530734No Hit
GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG5550.18957831633960137No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC5510.1882119861317484No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA5500.18787040357978513No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG5340.18240508274837322No Hit
CTATAAATGTATATGATCCATAATATAAGCCTCGTCCGACATGAAGGAAT5130.17523184915714504No Hit
GGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTA5120.1748902666051818No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5100.17420710150125532No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT5010.1711328585335861No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4940.16874178066984338No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT4830.1649843725982477No Hit
GTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTT4810.1643012074943212No Hit
AATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAAT4810.1643012074943212No Hit
ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA4760.16259329473450496No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA4720.16122696452665197No Hit
GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG4700.16054379942272548No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4610.15746955645505628No Hit
TTCTAGGAGTCTGCCTAATAGTCCAAATCATTACAGGTCTTTTCTTAGCC4480.15302898327953407No Hit
TTATAGCAGAGTACACTAACATTATTCTAATAAACGCCCTAACAACTATT4430.15132107051971785No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC4420.15097948796775462No Hit
ACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTAT4410.15063790541579136No Hit
CTATTTTGCCACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCT4290.1465389147922324No Hit
GGTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATC4280.14619733224026918No Hit
ATTTTGCCACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCG4230.14448941948045294No Hit
ATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGT4220.14414783692848968No Hit
GTACACTAACATTATTCTAATAAACGCCCTAACAACTATTATCTTCCTAG4190.14312308927259995No Hit
GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG4110.14039042885689398No Hit
TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA4080.13936568120100426No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC4050.1383409335451145No Hit
ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG4000.1366330207852983No Hit
GAGTACACTAACATTATTCTAATAAACGCCCTAACAACTATTATCTTCCT4000.1366330207852983No Hit
CTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGG3980.1359498556813718No Hit
GTACATGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTAT3980.1359498556813718No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA3930.13424194292155558No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA3910.1335587778176291No Hit
GGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGG3880.13253403016173934No Hit
GACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTTCGGGG3860.13185086505781285No Hit
CAGTAGAAGTACTCCAATGTTTCAGGTTTCTATAAATGTATATGATCCAT3860.13185086505781285No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3840.13116769995388636No Hit
ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG3820.13048453484995987No Hit
AAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACC3800.12980136974603337No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3610.12331130125873171No Hit
CCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAAA3580.12228655360284196No Hit
TACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACT3560.12160338849891547No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT3480.1188707280832095No Hit
CTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAA3460.11818756297928303No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA3450.11784598042731977No Hit
GTACATGGGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACAT3450.11784598042731977No Hit
GTATATGATCCATAATATAAGCCTCGTCCGACATGAAGGAATAAGCAAAT3420.11682123277143004No Hit
TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA3400.11613806766750355No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3360.11477173745965057No Hit
CTTTATAGCAGAGTACACTAACATTATTCTAATAAACGCCCTAACAACTA3360.11477173745965057No Hit
GGCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTG3340.11408857235572407No Hit
GTTTTTAGGTAGCTCGTTTGGTTTCGGGGTTTCTAGCTGTAATTCTTTTA3300.11272224214787109No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3300.11272224214787109No Hit
AACTAGAAAACTTCTAACTAAAAGAATTACAGCTAGAAACCCCGAAACCA3250.11101432938805486No Hit
ATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATA3240.1106727468360916No Hit
TTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGC3220.10998958173216511No Hit
GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG3190.10896483407627539No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3180.10862325152431213No Hit
ACATAATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAA3170.1082816689723489No Hit
AGTTAAACTTATTATCAAACAAATAATGCTAATCCACACACCAAAAGGAC3140.10725692131645917No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA3090.10554900855664293No Hit
GTAAAAGGCCTAGGAGATTTGTTGATCCAATAAATATGATTAGGGAAACA3080.1052074260046797No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3070.10486584345271643No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA3070.10486584345271643No Hit
TAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACAAC3060.1045242609007532No Hit
TAGTAGTAGAGCTTCTATTATGAAGTTAGTTGAGTAGAGTTCTGGTAAAT2970.10145001793308399No Hit
CTATAGCTCCTAGATGTACGAATTTCTTTCTCCAAAACATTTATCCTACT2970.10145001793308399No Hit
AGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTTCGGGGTTTC2970.10145001793308399No Hit
GTATGGTGGTACTCCCGCTGTAAAAATTGGTAAAGAAATATGTCACATAC2960.10110843538112073No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA2960.10110843538112073No Hit
GTTTAGATTATAGCCAAAAGAGGGACAGCTCTTCTGGAACGGAAAAAACC2930.100083687725231No Hit
TCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTA2930.100083687725231No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTATT353.363035E-668.0179141
AGGCATT750.063.3421176
CTTTAGG656.6029315E-1058.5297622
CATTCCG900.058.063619
GGCATTC900.058.063617
GTTGCTA451.5119194E-552.78519
GATCTTC558.1617327E-751.825377
CCTTTAG752.3264874E-950.786711
TAACTTT8950.049.895196
GTATGGT707.748167E-847.612541
ATGGGCA601.4852576E-647.5147255
ATAACTT9700.046.5350385
AACTTTA9800.046.0523077
TATAACT9950.045.8433534
ACTTTAT10150.044.46438
GTACCAG652.5409809E-643.9500351
GAATATA652.5409809E-643.9500351
TGGGCAG652.5802492E-643.8522386
CTATAAC10450.043.634953
GCTAGAT459.5582334E-442.2714962
Produced by FastQC (version 0.11.5)