FastQCFastQC Report
Fri 10 Feb 2017
SRR2031590_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031590_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences292755
Sequences flagged as poor quality0
Sequence length101
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC39771.3584738091578281No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22400.7651449163976703No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21890.7477242062475449No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC21560.7364519820327577No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA21120.721422349746375No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC17860.6100664378063568No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT14190.4847056412358457No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT13820.4720670868132055No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG13790.47104233915731586No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA12970.44303256989632966No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12660.4324435107854691No Hit
GTACTAGTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAAAACATT12430.4245871120903144No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12240.4180970436030128No Hit
GTTCTATAGCTCCTAGATGTACGAATTTCTTTCTCCAAAACATTTATCCT12230.41775546105104955No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC11920.4071664019401889No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA11360.38803777903024717No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA11330.3870130313743574No Hit
TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT11330.3870130313743574No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG11260.3846219535106147No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA10850.37061706888012164No Hit
ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA10820.36959232122423186No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10460.35729534935355506No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC10400.35524585404177556No Hit
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC10260.3504636983142901No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC10160.34704787279465765No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA9660.3299687451964954No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT9300.3176717733258185No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT8900.3040084712472887No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC8760.2992263155198033No Hit
GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT8650.29546890744820753No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA8170.2790729449539718No Hit
GTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTT8160.2787313624020085No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA8080.27599870198630255No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC7940.2712165462588171No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC7930.2708749637068538No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7660.26165223480384625No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT7560.25823640928421376No Hit
AGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTA7500.25618691397243426No Hit
AGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAAT7490.25584533142047106No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT7340.2507215931410223No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA7160.24457310720568393No Hit
GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG7080.24184044678997796No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC6720.22954347491930113No Hit
TTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTT6690.22851872726341138No Hit
AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG6650.2271523970555584No Hit
ACATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAG6550.22373657153592597No Hit
GTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTAT6460.22066232856825674No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC6260.21383067752899182No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG6160.2104148520093594No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT6110.20870693924954314No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC6090.20802377414561665No Hit
GTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCTT5990.2046079486259842No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC5950.2032416184181312No Hit
GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC5810.19845946269064574No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC5790.19777629758671927No Hit
GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT5490.1875288210278219No Hit
TTCTAGGAGTCTGCCTAATAGTCCAAATCATTACAGGTCTTTTCTTAGCC5480.18718723847585864No Hit
GGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGG5460.18650407337193214No Hit
ATTTTAAACTTGCTAAAAAAACAACAAAATCAAAAAGTAAGTTTAGATTA5170.17659817936499803No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5160.1762565968130348No Hit
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGT5140.1755734317091083No Hit
GGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTA4850.16566753770217418No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT4800.16395962494235794No Hit
CTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAA4790.1636180423903947No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT4770.16293487728646822No Hit
ATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGT4730.16156854707861523No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4700.16054379942272548No Hit
ATTTTGCCACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCG4680.159860634318799No Hit
TTATAGCAGAGTACACTAACATTATTCTAATAAACGCCCTAACAACTATT4670.15951905176683573No Hit
CTATTTTGCCACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCT4660.1591774692148725No Hit
ACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTAT4610.15746955645505628No Hit
ATTTACACCAACTTCCTAAACTTAAAATTGGGTTAATCTATAACTTTATA4590.1567863913511298No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT4580.15644480879916653No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT4550.1554200611432768No Hit
ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA4550.1554200611432768No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG4550.1554200611432768No Hit
ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC4530.1547368960393503No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC4450.15200423562364435No Hit
CTTTATAGCAGAGTACACTAACATTATTCTAATAAACGCCCTAACAACTA4250.14517258458437943No Hit
TATTAAACCCGAATGATATTTCCTATTTGCATACGCCATTCTACGCTCAA4230.14448941948045294No Hit
CTATAAATGTATATGATCCATAATATAAGCCTCGTCCGACATGAAGGAAT4200.14346467182456318No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA4170.14243992416867346No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC4120.14073201140885724No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA4110.14039042885689398No Hit
AATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACCTTTTGCATAAT4080.13936568120100426No Hit
CCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAAA4020.13731618588922478No Hit
TTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGC4000.1366330207852983No Hit
CTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGG3960.1352666905774453No Hit
GGTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATC3930.13424194292155558No Hit
TATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTAT3880.13253403016173934No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3870.1321924476097761No Hit
ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG3760.1284350395381804No Hit
GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG3760.1284350395381804No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC3700.12638554422640091No Hit
ACATAATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAA3700.12638554422640091No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3690.12604396167443765No Hit
CTATAGCTCCTAGATGTACGAATTTCTTTCTCCAAAACATTTATCCTACT3690.12604396167443765No Hit
GGTGTAAATTATGGAATTAATTGAAATTTTATGTTGAGCTTGAACGCTTT3690.12604396167443765No Hit
GTACATGGGGTATCAACGCAGAGTACATGGGGTATCAACGCAGAGTACAT3670.12536079657051116No Hit
CAGTAGAAGTACTCCAATGTTTCAGGTTTCTATAAATGTATATGATCCAT3670.12536079657051116No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT3620.12365288381069495No Hit
GTACACTAACATTATTCTAATAAACGCCCTAACAACTATTATCTTCCTAG3620.12365288381069495No Hit
GTACATGGGATGACAAACATACGAAAAACACACCCATTATTTAAAATTAT3600.12296971870676845No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG3590.12262813615480522No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT3490.11921231063517276No Hit
TAGTAGTAGAGCTTCTATTATGAAGTTAGTTGAGTAGAGTTCTGGTAAAT3450.11784598042731977No Hit
GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG3390.11579648511554029No Hit
ATATTAGGCTTCGTTGCTTTGAGGTATGAAGGAAAGGTATTAGGGCTAAA3380.11545490256357706No Hit
GACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTTCGGGG3380.11545490256357706No Hit
ACACTACACATCAGATACAATAACAGCCTTTTCATCAGTAACACACATTT3370.1151133200116138No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC3360.11477173745965057No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3320.11340540725179758No Hit
GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG3300.11272224214787109No Hit
TACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACT3280.1120390770439446No Hit
AATCAAAACATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACAT3230.11033116428412837No Hit
CATAATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAG3220.10998958173216511No Hit
AGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTTCGGGGTTTC3220.10998958173216511No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC3210.10964799918020188No Hit
TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA3160.10794008642038563No Hit
GGCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTG3160.10794008642038563No Hit
TCCTTTTGGTGTGTGGATTAGCATTATTTGTTTGATAATAAGTTTAACTA3150.1075985038684224No Hit
GAGTACACTAACATTATTCTAATAAACGCCCTAACAACTATTATCTTCCT3120.10657375621253268No Hit
GGAAAGATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTT3100.10589059110860619No Hit
TCTCACTATTTTGCCACATAGACGAGTTGATTCATAAAATTGTTTTTAGG3090.10554900855664293No Hit
CTACACATCAGATACAATAACAGCCTTTTCATCAGTAACACACATTTGTC3090.10554900855664293No Hit
GCCATACACTACACATCAGATACAATAACAGCCTTTTCATCAGTAACACA3070.10486584345271643No Hit
GAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGT3070.10486584345271643No Hit
TAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTTCGG3040.1038410957968267No Hit
TTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGT3040.1038410957968267No Hit
ATTCTTTATAGCAGAGTACACTAACATTATTCTAATAAACGCCCTAACAA3030.10349951324486345No Hit
GTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTACTTTT3030.10349951324486345No Hit
GTATATATCGGATTAGTCACCCGTAATTTACGTCTCGACAAATGTGTGTT3020.1031579306929002No Hit
CCTTTAATAGTGAATAATTAACAAAACAGCTTTTAACCATTGTAGGCCTA3020.1031579306929002No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA3000.10247476558897371No Hit
ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG2990.10213318303701047No Hit
GCCTAATAGTCCAAATCATTACAGGTCTTTTCTTAGCCATACACTACACA2990.10213318303701047No Hit
ACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGGTAAAA2960.10110843538112073No Hit
CTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGT2930.100083687725231No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGT600.079.181761
ATGGTGG650.073.065893
TGTGCTA200.002073770271.2392358
GTTGTGC200.002073770271.2392356
AGTTGTG200.002073770271.2392355
CGAAGAG200.002073770271.2392359
ATGTATT250.00501376357.0108641
GCTTGCA250.005020549556.991399
GTGCTAC250.005020549556.991399
CTCAATT352.7865818E-454.2775159
TATGGTG903.6379788E-1252.769812
ACCTGGG451.5145233E-552.769813
TACCTGG451.5145233E-552.769812
CCGGTGT903.6379788E-1252.769813
GTGGTAC903.6379788E-1252.769816
TCCGGTG957.2759576E-1249.992452
TTCCGGT1001.2732926E-1147.5090521
TCTCGAG601.4899942E-647.492838
CTCGAGT601.4899942E-647.492839
GGTACTC1001.2732926E-1147.4928258
Produced by FastQC (version 0.11.5)