FastQCFastQC Report
Fri 10 Feb 2017
SRR2031589_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031589_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242640
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC89110.7171022983325823No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC79220.6375136805510848No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT78730.6335704628854697No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC75290.6058874653962532No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA49940.4018863065731024No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC48310.3887690722976888No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT44440.35762570012232026No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC43890.353199639477242No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA43230.3478883667031481No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT39950.3214929504924999No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC38230.3076514517478916No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA38040.30612244897959184No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA34230.27546191978368634No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG32330.26017189210068886No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT32270.25968904912122576No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG31080.2501126633618747No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT31010.24954934655250113No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT29070.23393742354986158No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC28550.2297527843945149No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC28380.22838472928603618No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT27690.22283203502221077No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA27540.22162492757355307No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC27460.22098113693426896No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT26720.215026073520891No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA24940.20070173179681966No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT24680.19860941221914635No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC24390.19627567115174144No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC24250.19514903753299426No Hit
CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT23930.19257387497585784No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC23900.1923324534861263No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC23630.19015966007854246No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA23340.18782591901113757No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC23230.18694070688212194No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA23230.18694070688212194No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA22860.18396317517543295No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA22230.17889332389107063No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG22190.17857142857142858No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA22190.17857142857142858No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT21870.17599626601429216No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC21820.17559389686473958No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG21790.17535247537500803No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG21760.1751110538852765No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT21360.171892100688856No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA21200.17060451941028776No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC21120.16996072877100366No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT21040.16931693813171958No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT19950.16054529067147363No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC19600.15772870662460567No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT19370.15587780853666386No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT19270.15507307023755876No Hit
TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT19230.1547511749179167No Hit
TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA18660.15016416661301743No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA18470.1486351638447177No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC18410.14815232086525462No Hit
ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT18250.14686473958668642No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA18220.14662331809695486No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC18120.14581857979784973No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT18030.14509431532865513No Hit
GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG17930.14428957702955No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT17870.14380673405008693No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA17640.14195583596214512No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC17570.1413925191527715No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC17230.13865640893581407No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.13849546127599305No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG17120.13777119680679842No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG16950.1364031416983197No Hit
GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT16830.13543745573939356No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC16420.13213802871306252No Hit
ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA16330.13141376424386791No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG16050.12916049700637353No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA16020.12891907551664197No Hit
GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA16000.12875812785682095No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC15970.12851670636708942No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC15890.12787291572780532No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG15890.12787291572780532No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC15630.12578059615013198No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT15610.12561964849031093No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC15590.12545870083048993No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15540.12505633168093735No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA15270.12288353827335352No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC15260.12280306444344298No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT15130.12175690465460631No Hit
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC15110.1215959569947853No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA14900.11990600656666452No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG14870.11966458507693299No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA14730.11853795145818578No Hit
GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC14720.11845747762827528No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG14710.11837700379836477No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA14650.1178941608189017No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT14510.11676752720015451No Hit
ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG14440.11620421039078091No Hit
TAGTAGTAGAGCTTCTATTATGAAGTTAGTTGAGTAGAGTTCTGGTAAAT14420.11604326273095988No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA14310.11515805060194424No Hit
ATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGC14180.11411189081310757No Hit
CTTTATAGCAGAGTACACTAACATTATTCTAATAAACGCCCTAACAACTA14170.11403141698319706No Hit
GAGTACACTAACATTATTCTAATAAACGCCCTAACAACTATTATCTTCCT14070.11322667868409193No Hit
GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT13930.11210004506534475No Hit
ACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGGTAAAA13920.11201957123543424No Hit
GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG13850.11145625442606064No Hit
ACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAG13830.11129530676623961No Hit
GGCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTG13800.11105388527650809No Hit
ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA13740.11057104229704501No Hit
ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT13480.10847872271937166No Hit
ACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCAT13390.10775445825017704No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT13390.10775445825017704No Hit
TTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGT13220.10638640314169832No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT13200.10622545548187728No Hit
GTACACTAACATTATTCTAATAAACGCCCTAACAACTATTATCTTCCTAG13150.10582308633232473No Hit
GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT13020.10477692654348805No Hit
GTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTT13010.10469645271357754No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT12910.1038917144144724No Hit
GAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATA12700.10220176398635164No Hit
GGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTA12630.10163844717697805No Hit
ATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGCTTCA12540.10091418270778342No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG12520.10075323504796241No Hit
TGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCTATTGTGCTTG12470.10035086589840983No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGTAC156.537916E-495.291591
ACAGGGG301.360906E-679.230173
ATGCACT200.002066867271.312914
TACAGGG407.4598574E-659.489862
GGGCATC407.522627E-659.4058467
ATTGTCA250.00500945757.0342148
GTACAGG451.4887828E-552.9397771
GGAACAT902.2555469E-1047.528518
TAACTTT19100.046.5312736
TGGGAAC1250.045.625536
ACATATA3450.045.522682
GTTATAT953.7289283E-1045.1381231
AACTTTA20200.043.9956177
CAGGGGT652.5738627E-643.884874
GGGCATT652.5803038E-643.868937
CCATACT554.9377522E-543.2199484
TAAGGAC459.5836475E-442.2595025
GTACTAA1350.042.2475669
GGGATGC1253.6379788E-1241.8217127
ACTTTAT21650.041.271878
Produced by FastQC (version 0.11.5)