FastQCFastQC Report
Fri 10 Feb 2017
SRR2031589_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031589_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242640
Sequences flagged as poor quality0
Sequence length101
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC89230.7180679842915084No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC82530.6641505182514646No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT79140.6368698899118006No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC76560.6161076417948883No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA53870.4335125217279341No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC46840.37693941930084335No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT45900.3693748792892551No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC43440.3495783171312689No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA40680.3273675400759673No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT40560.32640185411704115No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC39560.3183544711259898No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA38250.3078123994077126No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA35620.2866477821412477No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG33370.26854117041138226No Hit
CCCCTATACTATATCAATTTACCAGAACTCTACTCAACTAACTTCATAAT32050.25791862486319445No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT31420.2528487735788322No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG31300.251883087619906No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC28540.2296723105646044No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC27990.2252462499195262No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC27530.22154445374364257No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA27450.22090066310435844No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT27380.2203373462949849No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT26380.21228996330393354No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT25760.20730058584948174No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA25300.20359878967359815No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC25120.20215026073520892No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC24690.19868988604905685No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA24520.1973218309405781No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA24360.1960342496620099No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT24220.19490761604326273No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC23800.1915277151870212No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC23460.18879160497006373No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA23330.18774544518122707No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC23320.18766497135131655No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC23100.18589454709328526No Hit
CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT23040.18541170411382218No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA22760.18315843687632782No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC22740.1829974892165068No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG22620.18203180325758064No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT22320.17961758836026523No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG21350.17181162685894547No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT20880.16802935685315135No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT20870.16794888302324085No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT20850.1677879353634198No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA20810.16746604004377777No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG20350.16376424386789415No Hit
TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA20280.16320092705852057No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA20170.16231571492950492No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20050.16135002897057876No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT19980.1607867121612052No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT19920.1603038691817421No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC19680.1583724972638898No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC19280.15515354406746926No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19260.15499259640764823No Hit
ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT19240.1548316487478272No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT18700.1504860619326595No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA18600.14968132363355435No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT18460.1485546900148072No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC18430.14831326852507565No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC18340.14758900405588105No Hit
TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT17640.14195583596214512No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA17610.14171441447241356No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC17530.14107062383312946No Hit
GCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGG17340.13954162106482973No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC17270.13897830425545613No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG17080.13744930148715637No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG16980.13664456318805127No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA16840.13551792956930406No Hit
ATGCTAGTGTTAGGGGTAGAAAGTTTTTTCATAGAAGATGTATAAGTTGA16630.1338279791411833No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG16490.1327013455224361No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG16390.131896607223331No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC16300.13117234275413636No Hit
GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT16160.13004570913538918No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC16150.12996523530547865No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG16100.1295628661559261No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC15940.12827528487735787No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA15800.12714865125861072No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA15700.12634391295950556No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA15630.12578059615013198No Hit
TAGTAGTAGAGCTTCTATTATGAAGTTAGTTGAGTAGAGTTCTGGTAAAT15580.1253782270005794No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC15580.1253782270005794No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT15500.12473443636129532No Hit
GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA15330.1233663812528166No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA15200.1223202214639799No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA15160.12199832614433787No Hit
ATATAATGATGGCTACAACGATTGGGAATCCTATTATTGTTGGGGTAATG15130.12175690465460631No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC14980.12054979720594863No Hit
GATTATATATGAATCAAAGCATAGGTCTTCATAGTCAGTATATTCGTAGC14880.11974505890684349No Hit
ATCAACAACCGTCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTAT14840.11942316358720144No Hit
ACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAG14610.11757226549925963No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG14600.11749179166934912No Hit
GAGTACACTAACATTATTCTAATAAACGCCCTAACAACTATTATCTTCCT14240.11459473379257065No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA14220.11443378613274964No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT14150.11387046932337605No Hit
CTTTATAGCAGAGTACACTAACATTATTCTAATAAACGCCCTAACAACTA14080.11330715251400243No Hit
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC14040.1129852571943604No Hit
ACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTGGTAAAA13950.11226099272516578No Hit
ACTTAACCCTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCAT13930.11210004506534475No Hit
GTACACTAACATTATTCTAATAAACGCCCTAACAACTATTATCTTCCTAG13770.11081246378677653No Hit
ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA13730.11049056846713448No Hit
GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT13640.10976630399793988No Hit
ATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGC13460.10831777505955063No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT13410.10791540590999807No Hit
TTATTAGGGTTCATGTTCGTCCTTTTGGTGTGTGGATTAGCATTATTTGT13320.10719114144080344No Hit
GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG13320.10719114144080344No Hit
CTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTC13290.10694971995107191No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT13270.10678877229125089No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT13140.10574261250241421No Hit
AATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACCATCCT13060.1050988218631301No Hit
GGCTATACTTAGATTTATGGATAGTTGGGTAGTAGGTGTAAATGTATGTG13010.10469645271357754No Hit
TGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCTATTGTGCTTG12850.10340887143500932No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA12830.10324792377518831No Hit
GTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTT12820.1031674499452778No Hit
TTATAGCAGAGTACACTAACATTATTCTAATAAACGCCCTAACAACTATT12570.10115560419751497No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT12510.1006727612180519No Hit
GAATTAAAATGAACGAAAATCTATTTGCCTCATTCATTACCCCAACAATA12490.10051181355823086No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG12480.10043133972832034No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTATT950.080.0464251
AAGACAT903.6379788E-1252.7701575
GGGCATC654.4248736E-851.1464587
TAACTTT19250.049.8369566
CATTCCG3400.048.899
GGCATTC3400.048.897
CCTTTAG3500.047.527561
CTTTAGG3450.046.8161352
TGGGATA1550.045.96116
AACTTTA21100.045.692457
AAAGACA1052.0008883E-1145.2406654
GGAACAT554.9677787E-543.1755838
ACTTTAT22700.042.0533948
GTATTAC1051.022272E-940.7379151
GGAGTGA1800.039.5776186
CATGGGT5600.039.0200734
ATCTATA25050.038.325621
ATAACTT25500.038.180765
GCCTTTA500.001617013837.994517
GGGATAT500.001617013837.994517
Produced by FastQC (version 0.11.5)