##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031588_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 306823 Sequences flagged as poor quality 0 Sequence length 101 %GC 38 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.87116024548355 33.0 31.0 34.0 30.0 34.0 2 31.96036151135997 33.0 31.0 34.0 30.0 34.0 3 32.05325871919641 34.0 31.0 34.0 30.0 34.0 4 35.09362075202967 37.0 35.0 37.0 33.0 37.0 5 35.04778324962601 37.0 35.0 37.0 32.0 37.0 6 35.395827561819026 37.0 35.0 37.0 33.0 37.0 7 35.54401071627616 37.0 35.0 37.0 33.0 37.0 8 35.5827007753656 37.0 36.0 37.0 33.0 37.0 9 37.36351577293749 39.0 38.0 39.0 35.0 39.0 10-11 37.357613347108924 39.0 38.0 39.0 35.0 39.0 12-13 37.41800321357917 39.0 38.0 39.0 35.0 39.0 14-15 38.53626031946757 40.0 38.0 41.0 34.5 41.0 16-17 38.598589414744005 40.0 38.0 41.0 34.5 41.0 18-19 38.725858230967035 41.0 38.5 41.0 35.0 41.0 20-21 38.74985089123045 41.0 39.0 41.0 35.0 41.0 22-23 38.534215166398866 40.5 38.5 41.0 34.5 41.0 24-25 38.31074430534869 40.0 38.0 41.0 34.0 41.0 26-27 38.007807432949946 40.0 37.5 41.0 34.0 41.0 28-29 37.78413938981106 40.0 37.0 41.0 34.0 41.0 30-31 37.469462849916724 40.0 36.5 41.0 33.0 41.0 32-33 36.968933228604115 40.0 35.0 41.0 32.5 41.0 34-35 36.57015119466272 39.5 35.0 41.0 31.0 41.0 36-37 36.1714604185475 39.5 35.0 41.0 29.5 41.0 38-39 35.713310279868196 39.0 35.0 41.0 26.0 41.0 40-41 35.48698598214605 39.0 35.0 41.0 23.5 41.0 42-43 35.005317397978644 39.0 35.0 41.0 18.5 41.0 44-45 34.387027700009455 39.0 35.0 41.0 13.5 41.0 46-47 33.71461233349521 38.5 34.5 41.0 9.5 41.0 48-49 33.34583945792851 38.5 33.5 41.0 8.0 41.0 50-51 32.839345485833846 38.5 33.0 40.5 8.0 41.0 52-53 32.980464306782736 38.5 33.0 40.5 7.5 41.0 54-55 33.068531368248145 39.0 33.0 41.0 7.0 41.0 56-57 32.733308780632484 39.0 32.5 41.0 7.0 41.0 58-59 32.4524351173152 39.0 31.5 41.0 7.0 41.0 60-61 31.8838092320328 38.0 30.0 41.0 7.0 41.0 62-63 31.610811445035086 38.0 29.0 41.0 7.0 41.0 64-65 31.32974222923314 37.5 28.0 40.5 7.0 41.0 66-67 30.86728504707926 37.0 26.5 39.5 7.0 41.0 68-69 30.35987034870267 36.0 26.0 39.0 7.0 41.0 70-71 29.77700661293319 35.0 25.0 38.5 7.0 41.0 72-73 29.296496677237364 35.0 23.0 37.0 7.0 40.0 74-75 28.937693719180114 35.0 21.5 37.0 7.0 39.5 76-77 28.560446902611602 35.0 20.0 36.5 7.0 39.0 78-79 28.126664233124636 35.0 19.5 36.0 7.0 39.0 80-81 27.675751165981687 35.0 18.0 36.0 7.0 37.5 82-83 27.246008284906935 34.5 16.5 35.0 7.0 37.0 84-85 26.981321478507155 34.0 15.0 35.0 7.0 36.5 86-87 26.913468025539153 34.0 14.0 35.0 7.0 36.0 88-89 26.637354435619233 34.0 11.5 35.0 7.0 36.0 90-91 26.377434221032974 34.0 10.5 35.0 7.0 35.5 92-93 26.159507272922824 34.0 10.0 35.0 7.0 35.0 94-95 25.940851891807327 34.0 10.0 35.0 7.0 35.0 96-97 25.752824918601277 34.0 10.0 35.0 7.0 35.0 98-99 25.822516564925053 34.0 10.0 35.0 7.0 35.0 100-101 25.351002043523465 33.0 10.0 35.0 7.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.5348060284586005 1101 2 0.4636267425192315 1101 3 0.4021425683728381 1101 4 0.6300420570893195 1101 5 0.45954650910645256 1101 6 0.2319970750185263 1101 7 0.1670280061549576 1101 8 0.33332339198586425 1101 9 0.3461027531037004 1101 10-11 0.36614925839271706 1101 12-13 0.10261557253911491 1101 14-15 0.3977849870638792 1101 16-17 0.095418610801147 1101 18-19 0.23958353930157017 1101 20-21 -0.17866383045444678 1101 22-23 -0.029564787634207335 1101 24-25 0.23612544945070368 1101 26-27 0.22289507513747253 1101 28-29 0.45508592439836804 1101 30-31 0.3075455098699962 1101 32-33 0.04422050168768976 1101 34-35 -0.15627668188791688 1101 36-37 -0.39539934326761284 1101 38-39 -0.36464095093709403 1101 40-41 -0.5307139113403423 1101 42-43 -0.5000223439896203 1101 44-45 -0.5572986307849987 1101 46-47 -0.04645385740547425 1101 48-49 0.15596040139323009 1101 50-51 0.2005327137258206 1101 52-53 0.35723282291821334 1101 54-55 0.3932244382628056 1101 56-57 0.5957794778542151 1101 58-59 0.3707110420537063 1101 60-61 0.18960822282479484 1101 62-63 -0.3142922546855864 1101 64-65 -0.22835953742202975 1101 66-67 0.07060091751742448 1101 68-69 0.020183342031014462 1101 70-71 0.14239694510353829 1101 72-73 0.288884816278113 1101 74-75 0.38569022827764243 1101 76-77 0.41728376387869304 1101 78-79 0.0946124681386955 1101 80-81 0.3819624547040341 1101 82-83 0.09236220191329281 1101 84-85 0.3434460175222327 1101 86-87 0.4214189467530396 1101 88-89 0.1921770657657369 1101 90-91 0.2522987633064524 1101 92-93 0.2573843020590374 1101 94-95 0.510228094769591 1101 96-97 0.7721115293876863 1101 98-99 0.8534296230167193 1101 100-101 1.0985390912550734 1102 1 0.16647784945186928 1102 2 0.14805461226636396 1102 3 0.11824225911324504 1102 4 0.07772261011824355 1102 5 0.5728082882587202 1102 6 0.5493883793663557 1102 7 0.35594923591664696 1102 8 0.22986696120543826 1102 9 0.1952932206314415 1102 10-11 0.16882344431231644 1102 12-13 0.4465395310619513 1102 14-15 0.33494161795345434 1102 16-17 0.16628453767035012 1102 18-19 -0.032010057205610565 1102 20-21 0.1714180323798189 1102 22-23 -0.14088914637534344 1102 24-25 -2.671283189812357E-4 1102 26-27 0.30397566454988123 1102 28-29 0.06422727422555141 1102 30-31 -0.14602916722660098 1102 32-33 0.1701619242818353 1102 34-35 0.4125972493473 1102 36-37 0.34406763872256363 1102 38-39 0.13153878346348336 1102 40-41 0.38339378376608124 1102 42-43 -0.012319960871558067 1102 44-45 0.1851245091094924 1102 46-47 -0.11202598765834182 1102 48-49 0.6874321132360492 1102 50-51 -0.023098357767722177 1102 52-53 0.1626266741097666 1102 54-55 0.14173271550494349 1102 56-57 -0.08611064403044821 1102 58-59 0.16347072915575467 1102 60-61 0.25353400052171793 1102 62-63 -0.2600717707852773 1102 64-65 0.0721316395836098 1102 66-67 0.2762403572118046 1102 68-69 0.5152169967517715 1102 70-71 0.5076871033716834 1102 72-73 0.5829361169875931 1102 74-75 0.5457047816704055 1102 76-77 0.6239510369367238 1102 78-79 0.21060300843340585 1102 80-81 0.7244947450442218 1102 82-83 0.5591258911983594 1102 84-85 0.5825855481753166 1102 86-87 0.13529014922711724 1102 88-89 0.06327948526728377 1102 90-91 0.33176938364481856 1102 92-93 0.4514901779913636 1102 94-95 0.4779013711021314 1102 96-97 0.06898254994435504 1102 98-99 0.2735769761184095 1102 100-101 0.12897387386376735 1103 1 -0.18192762152313335 1103 2 -0.485660798584167 1103 3 -0.06976097565346606 1103 4 -0.09359696373640247 1103 5 -0.058867813144182435 1103 6 0.024178295332738742 1103 7 0.011200457744287462 1103 8 -0.2244771195230868 1103 9 -0.03734627868292506 1103 10-11 -0.032444091990910806 1103 12-13 -0.001220744596430734 1103 14-15 -0.11279228732413316 1103 16-17 -0.15785139285248562 1103 18-19 -0.11699300435936522 1103 20-21 -0.22775042161265446 1103 22-23 -0.37426701635178006 1103 24-25 -0.13524831744735621 1103 26-27 -0.1230311214281059 1103 28-29 -0.33311283336560393 1103 30-31 -0.06651367902295391 1103 32-33 0.01354823278012418 1103 34-35 0.08517381135285973 1103 36-37 0.2478596994799247 1103 38-39 0.20897980171178432 1103 40-41 0.38327951211600464 1103 42-43 -0.030840758311867944 1103 44-45 0.6857046574868306 1103 46-47 0.9685443157768461 1103 48-49 1.0115504635050243 1103 50-51 1.0186547451470815 1103 52-53 0.831748716232056 1103 54-55 1.1619675876964948 1103 56-57 1.129265045994508 1103 58-59 0.9607000811475999 1103 60-61 0.9983038749987045 1103 62-63 0.9750025936885471 1103 64-65 1.0216587307548046 1103 66-67 1.002058718028934 1103 68-69 1.386898723835909 1103 70-71 1.0891452096267393 1103 72-73 0.9190090398805957 1103 74-75 0.6258619491399031 1103 76-77 0.44653111499305354 1103 78-79 0.79221933697319 1103 80-81 1.0100771788545622 1103 82-83 0.6614341785300262 1103 84-85 0.5221270551546766 1103 86-87 0.7617185448531494 1103 88-89 1.0138613916700088 1103 90-91 1.1941511564376164 1103 92-93 0.7735516385588532 1103 94-95 0.6183939698152621 1103 96-97 0.9423344011450858 1103 98-99 0.8622166160748108 1103 100-101 1.061746983107465 1104 1 0.1663450208671584 1104 2 0.22232239469314408 1104 3 0.2042819748600735 1104 4 -0.22361563787271876 1104 5 -0.11605735562784503 1104 6 0.11684869682667198 1104 7 0.3148885996677251 1104 8 0.010583571419957138 1104 9 0.07936707332454773 1104 10-11 0.04470343368602414 1104 12-13 -0.05000692573555199 1104 14-15 -0.2829603545510295 1104 16-17 -0.2965068550373573 1104 18-19 -0.03821149175433192 1104 20-21 0.1040656381445828 1104 22-23 0.20859946357352044 1104 24-25 0.07962096359839421 1104 26-27 0.1677018384225022 1104 28-29 0.20260145234868077 1104 30-31 0.09595130697144327 1104 32-33 -0.06966838720119739 1104 34-35 -0.09713244448280989 1104 36-37 -0.3358237739094321 1104 38-39 -0.36093149614068665 1104 40-41 -0.387028611133303 1104 42-43 -0.36230667801308414 1104 44-45 -0.4311254078726492 1104 46-47 -0.4782343957077515 1104 48-49 -0.8654336634791022 1104 50-51 -0.8333527245074492 1104 52-53 -0.8800860176615046 1104 54-55 -1.0630557549739024 1104 56-57 -1.3038582033052073 1104 58-59 -1.3568769496647235 1104 60-61 -0.9798707281758965 1104 62-63 -0.9180879758726128 1104 64-65 -0.7942843131294133 1104 66-67 -1.014164437900103 1104 68-69 -1.052176906416193 1104 70-71 -0.6630723440614013 1104 72-73 -0.7893242934527329 1104 74-75 -0.86900266337177 1104 76-77 -0.6977265880881838 1104 78-79 -1.0236069935590244 1104 80-81 -0.9802356129288157 1104 82-83 -1.1215957000880863 1104 84-85 -0.8794835891030246 1104 86-87 -0.903080708181399 1104 88-89 -0.7545793179471687 1104 90-91 -1.0837433346921692 1104 92-93 -1.336042198976159 1104 94-95 -1.5168768051613988 1104 96-97 -1.3780437501154204 1104 98-99 -1.3623398731875582 1104 100-101 -1.4761848562949638 1105 1 -0.11460108616590858 1105 2 -0.13439697289578945 1105 3 -0.0935096055402056 1105 4 -0.302369010104357 1105 5 -0.3728645580872225 1105 6 -0.14231122537673002 1105 7 -0.36459254720472245 1105 8 -0.43861336690741837 1105 9 -0.4839367725879953 1105 10-11 -0.39788236216063666 1105 12-13 -0.407463478844285 1105 14-15 -0.7170371473234738 1105 16-17 -0.4265181480921285 1105 18-19 -0.3060686346114707 1105 20-21 -0.20198398855721678 1105 22-23 -0.37561221846425497 1105 24-25 -0.2858113094425718 1105 26-27 -0.07829069561351787 1105 28-29 -0.08028956967277168 1105 30-31 -0.179411011869135 1105 32-33 0.28414145030428983 1105 34-35 0.26230039321090004 1105 36-37 0.16448207965729011 1105 38-39 -0.3804512276169376 1105 40-41 -0.20403406944310376 1105 42-43 -0.460101395373016 1105 44-45 -0.11302985608143956 1105 46-47 -0.030841209184131912 1105 48-49 -0.2610356460376053 1105 50-51 -0.24492183172873183 1105 52-53 0.20011819841228373 1105 54-55 0.4681256240335614 1105 56-57 0.13668856876329727 1105 58-59 -0.12652215952732604 1105 60-61 -0.045174385870861755 1105 62-63 0.15784213266619318 1105 64-65 0.09851793293369937 1105 66-67 -0.12663228406360005 1105 68-69 0.44824658313773114 1105 70-71 0.19575662889800682 1105 72-73 0.02248165422277637 1105 74-75 0.05723173025392825 1105 76-77 0.23724423818699236 1105 78-79 0.619908910826446 1105 80-81 0.33887945075146675 1105 82-83 0.12279698452198673 1105 84-85 0.021114001711957542 1105 86-87 0.33979839339335527 1105 88-89 0.1330861566748247 1105 90-91 0.0631683285238438 1105 92-93 0.4668704680669471 1105 94-95 0.06635457698302716 1105 96-97 -0.041722462707173946 1105 98-99 -0.3048707722402 1105 100-101 -0.1575873909583656 1106 1 -0.02796258723355649 1106 2 0.10259059392686254 1106 3 0.10439144393505728 1106 4 0.31921413771567586 1106 5 -0.13042517171979995 1106 6 0.009158494308884713 1106 7 -0.03828600350687594 1106 8 0.2909612397286594 1106 9 0.08073543676187 1106 10-11 0.21800666302373628 1106 12-13 0.4790023791358209 1106 14-15 0.3687911506487538 1106 16-17 0.42557853084112907 1106 18-19 0.41040646117397017 1106 20-21 0.16393153852772713 1106 22-23 0.26511287353520174 1106 24-25 0.13291871948235467 1106 26-27 0.03695471198571454 1106 28-29 0.08136445180133478 1106 30-31 0.2607022648258521 1106 32-33 0.2258296970899849 1106 34-35 -0.009283511806287947 1106 36-37 -0.022810637620438 1106 38-39 0.030078356076060686 1106 40-41 0.12514982345892633 1106 42-43 0.2481202513793619 1106 44-45 0.2539457470260942 1106 46-47 0.8884870054964082 1106 48-49 0.41316513236107966 1106 50-51 -0.25344029976743343 1106 52-53 -0.3585342935235687 1106 54-55 -0.18374541014631518 1106 56-57 -0.09839843319026187 1106 58-59 -0.03880086865761001 1106 60-61 0.5117138366845282 1106 62-63 0.6906969173950408 1106 64-65 0.6325766611453538 1106 66-67 0.33223676625971876 1106 68-69 0.21396978214428586 1106 70-71 0.10150072654615272 1106 72-73 0.3983467519769306 1106 74-75 0.3150422189489781 1106 76-77 0.21387713386036822 1106 78-79 -0.39032839476318415 1106 80-81 -0.06596358227747601 1106 82-83 0.054581092468016124 1106 84-85 -0.19304407076695895 1106 86-87 -0.39911245421314234 1106 88-89 -8.847366663822243E-4 1106 90-91 -0.020456525715701446 1106 92-93 0.0020119882159583824 1106 94-95 0.12303835630548932 1106 96-97 0.2131576729825575 1106 98-99 0.20033117224210528 1106 100-101 -0.10474260123201873 1107 1 0.13852633325465646 1107 2 0.34345641531169946 1107 3 -0.08887790077402968 1107 4 -0.2925487093831123 1107 5 -0.09171600396690849 1107 6 0.020178757373230383 1107 7 0.007533011384303734 1107 8 0.36032174847771614 1107 9 0.14768649731406924 1107 10-11 0.1478166721085401 1107 12-13 -0.029470188703967892 1107 14-15 0.26330866852212154 1107 16-17 -0.15328152366770098 1107 18-19 -0.04833667584858148 1107 20-21 -0.46051053596767844 1107 22-23 -0.0918014528067772 1107 24-25 -0.35928019824090995 1107 26-27 -0.6680656300769314 1107 28-29 -1.0509577032723243 1107 30-31 -0.8834301363275259 1107 32-33 -0.6983911821611102 1107 34-35 -0.688115916909581 1107 36-37 -0.3048345741058043 1107 38-39 -0.33894245997639416 1107 40-41 -0.28184942605720664 1107 42-43 -0.4651949123446215 1107 44-45 -0.10930819301419348 1107 46-47 -0.3118131880494346 1107 48-49 -0.17266652225834633 1107 50-51 -0.1854227621444977 1107 52-53 -0.3908248492766191 1107 54-55 -0.1707304399681817 1107 56-57 -0.013909749696964013 1107 58-59 -0.17677140229113064 1107 60-61 0.04985026675128523 1107 62-63 0.06198893461667154 1107 64-65 0.194649576577671 1107 66-67 -0.03553982654353405 1107 68-69 0.10307591636240332 1107 70-71 0.02271967033886213 1107 72-73 0.18692754748982665 1107 74-75 0.02499995485765183 1107 76-77 0.2450814629672955 1107 78-79 0.1948515238685644 1107 80-81 0.19401982807920248 1107 82-83 0.0997878705222881 1107 84-85 -0.11248555277509098 1107 86-87 0.04361377717288306 1107 88-89 -0.0326025547332911 1107 90-91 0.20346415998955436 1107 92-93 0.31632648045437506 1107 94-95 0.3877419151789745 1107 96-97 0.35385364801909347 1107 98-99 0.0587859654640539 1107 100-101 0.34375050616617386 1108 1 0.04320889773103076 1108 2 -0.05798063560988709 1108 3 -0.1403548697767718 1108 4 -0.18862285287993785 1108 5 -0.10174759131808031 1108 6 0.05780588778386431 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-1.396839174405116 2316 74-75 -1.5194242903558965 2316 76-77 -1.2204200404691328 2316 78-79 -1.3290385411780683 2316 80-81 -1.249704858960559 2316 82-83 -1.2902464937388807 2316 84-85 -1.9590966843411195 2316 86-87 -1.3229715811972724 2316 88-89 -1.0178481671535202 2316 90-91 -1.3559407553270901 2316 92-93 -1.4524856513571116 2316 94-95 -1.1122389083359998 2316 96-97 -1.1436687501154204 2316 98-99 -0.6351573335050205 2316 100-101 -1.0172314832790903 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 44.0 8 291.0 9 397.0 10 344.0 11 422.0 12 581.0 13 996.0 14 1646.0 15 2581.0 16 4334.0 17 5895.0 18 6470.0 19 8040.0 20 8764.0 21 8052.0 22 5741.0 23 5055.0 24 4732.0 25 4106.0 26 3991.0 27 4255.0 28 4381.0 29 4598.0 30 4767.0 31 5871.0 32 6872.0 33 8533.0 34 11806.0 35 19826.0 36 28326.0 37 46842.0 38 71075.0 39 17162.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 51.320585413139376 21.24857134436086 14.081877933042309 13.348965309457455 2 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14.507767797466704 20-21 18.21664749153415 30.83051681599369 32.228237311005444 18.724598381466716 22-23 16.09869533900653 31.502201595056434 33.797661844125116 18.601441221811925 24-25 19.865199153909582 31.481179702955775 31.912535892028952 16.741085251105687 26-27 17.926785149744315 27.658454548713753 39.01695765962786 15.397802641914069 28-29 14.531505134882327 36.18975761269527 35.12090032363936 14.157836928783043 30-31 16.70670060588678 32.07044452338971 33.42415659842972 17.798698272293798 32-33 15.75158967873986 32.82576599537844 36.02060471346672 15.402039612414974 34-35 18.798786270911894 31.798300648908327 32.26893029531684 17.133982784862933 36-37 17.415578362769416 30.428129573076333 36.2067054946989 15.949586569455354 38-39 15.866476763476012 34.14150829631416 34.60219735808593 15.389817582123896 40-41 15.21610179158668 34.95451775127679 32.76889281442395 17.060487642712573 42-43 14.521890471053345 34.21972929017708 29.677859873607908 21.580520365161675 44-45 18.43710986903839 29.959609971033412 30.883603280159928 20.71967687976827 46-47 19.705840564017894 31.442495031200956 32.62847428064691 16.223190124134238 48-49 17.0171884531697 31.896993244014705 32.252245198879116 18.83357310393648 50-51 12.530900836850103 31.90678546502906 35.44904867819428 20.113265019926555 52-53 16.35363512867806 35.0280578672107 28.727472574066738 19.890834430044503 54-55 15.211296549092138 29.362644603298087 31.09176815196455 24.334290695645226 56-57 18.738029124914757 28.984608758357176 30.473646944429035 21.80371517229903 58-59 22.76051054112466 28.35281164620968 28.891408065897323 19.99526974676834 60-61 18.640073506011532 29.546022042011362 34.28719242613902 17.52671202583808 62-63 14.811964974188069 30.93102659609227 33.01983271254002 21.23717571717964 64-65 14.654775698036849 33.15148745899901 30.427715856982 21.76602098598215 66-67 18.648884538521145 28.441453566621806 28.2900607691994 24.619601125657653 68-69 16.674415910911424 28.22388953329973 30.835792698743642 24.265901857045204 70-71 19.984309145712906 28.016493338726722 30.281391494969796 21.717806020590576 72-73 18.833895695766543 24.615003253493633 34.971713359872275 21.57938769086755 74-75 16.30970138292768 32.11251423890019 29.811769083390182 21.766015294781955 76-77 17.765198346527036 28.494807173760016 29.181249969377106 24.55874451033584 78-79 19.28174280021085 27.670331499556923 30.612261550588237 22.435664149643987 80-81 22.3341825500641 26.52149518686574 29.61994017425797 21.524382088812192 82-83 20.241598214926505 27.824409053245557 31.772970751153185 20.161021980674754 84-85 19.430075901792573 29.188668072799167 30.205911955225613 21.17534407018265 86-87 19.06088249779033 30.207235973086068 28.96264599307909 21.769235536044512 88-89 19.96333112580621 29.961145347718144 25.35437335047157 24.721150176004073 90-91 21.174610633070003 27.10297299149315 27.590313875591843 24.132102499845004 92-93 23.908625381366928 29.91483825471475 27.547873894668452 18.628662469249868 94-95 21.74372079057988 28.693441424505888 28.24942560246469 21.313412182449547 96-97 20.208943294975413 27.84948253504816 30.379474298017406 21.562099871959024 98-99 21.942022461647863 31.40863171835194 24.23923644749761 22.410109372502586 100-101 20.89730147522833 28.074017424881088 26.30951585051063 24.719165249379955 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1148.0 1 727.0 2 521.5 3 1008.0 4 828.5 5 457.5 6 771.0 7 1362.0 8 1642.5 9 1375.0 10 927.0 11 628.5 12 560.5 13 519.5 14 511.0 15 522.5 16 510.0 17 497.0 18 520.0 19 544.5 20 591.5 21 648.0 22 742.0 23 913.0 24 1144.5 25 1413.5 26 1768.0 27 2582.5 28 3645.5 29 4398.0 30 5631.0 31 7319.5 32 10043.5 33 12707.5 34 17621.0 35 22175.5 36 20456.5 37 16994.0 38 13339.0 39 10068.0 40 7723.0 41 5509.5 42 4114.5 43 3534.5 44 3583.0 45 3996.0 46 5487.0 47 14371.5 48 31640.0 49 30189.0 50 13552.0 51 5605.0 52 2499.5 53 1464.0 54 963.5 55 601.5 56 401.0 57 307.0 58 254.0 59 278.0 60 235.5 61 158.5 62 143.5 63 146.0 64 119.5 65 82.0 66 85.0 67 104.0 68 96.0 69 68.5 70 79.0 71 73.0 72 32.0 73 28.5 74 25.0 75 9.5 76 8.5 77 5.0 78 4.5 79 4.5 80 5.5 81 5.5 82 6.0 83 7.0 84 3.0 85 0.0 86 0.5 87 1.0 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 1.0 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.47779990417928253 2 0.37676445377302226 3 0.2046782672746176 4 0.2362925856275442 5 0.2568255965165584 6 0.20695971292895252 7 0.19848577192713718 8 0.19978945515818566 9 0.1991376135426614 10-11 0.2126633270647898 12-13 0.2010931383892342 14-15 0.21038188141045489 16-17 0.08799861809577508 18-19 0.03519944723831003 20-21 6.518416155242599E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.1419385117804076 46-47 0.2159225351424111 48-49 0.09125782617339638 50-51 0.0642063991291396 52-53 0.07316922134259818 54-55 0.08196908315217569 56-57 0.11407228271674548 58-59 0.0930503906160881 60-61 0.14780508632012596 62-63 0.24867757632250514 64-65 0.13949410572219162 66-67 0.10934643100419461 68-69 0.11130195585076737 70-71 0.08962822213458574 72-73 0.07479882538140882 74-75 0.1447088386463857 76-77 0.2214631888743673 78-79 0.1442199574347425 80-81 0.08669493486472657 82-83 0.09158374698115852 84-85 0.1005465691946171 86-87 0.07039889447662007 88-89 0.09647255909759046 90-91 0.1186351740254153 92-93 0.17061954286347503 94-95 0.13525713522128394 96-97 0.09142078657727745 98-99 0.08343572678710527 100-101 0.06273975549421003 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 306823.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 24.131828448323624 #Duplication Level Percentage of deduplicated Percentage of total 1 80.82169579427892 19.503752978101378 2 7.622700629372519 3.678994078018923 3 3.103643877799087 2.246898048712124 4 1.719294454498798 1.6595887531247657 5 1.135841819507847 1.3704969966397564 6 0.8171038059479755 1.1830925321765318 7 0.5469868453040166 0.9239854900056385 8 0.452445909078631 0.8734677648025083 9 0.41057778017881746 0.8917193300371875 >10 2.582318143756247 12.520247830182221 >50 0.40247427135949865 6.840751834119346 >100 0.30658275033089327 15.3355517676315 >500 0.03511520488371465 6.137414730968669 >1k 0.040517544096593826 20.523559185589086 >5k 0.0013505848032197942 2.421265680864863 >10k+ 0.0013505848032197942 3.8892129990254967 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 11933 3.8892129990254967 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 7429 2.421265680864863 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 4882 1.5911453834947182 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 3664 1.194173839640444 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3220 1.0494650009940585 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 3148 1.0259987028351851 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 3118 1.0162210786023211 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 2924 0.9529924418964679 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 2890 0.9419111344325555 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2763 0.900519191846765 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 2703 0.8809639433810372 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 2516 0.820016752329519 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 2400 0.7822099386291119 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 2170 0.7072481528438219 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2160 0.7039889447662007 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2023 0.6593377941027889 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 2011 0.6554267444096433 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1869 0.6091459897074208 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1844 0.6009979695133677 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 1708 0.5566727396577179 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 1695 0.5524357691568103 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 1545 0.5035476479924907 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 1460 0.4758443793327098 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 1324 0.43151914947706005 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 1250 0.40740100970266246 No Hit GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1213 0.39534193981546367 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 1212 0.3950160190077015 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 1137 0.37057195842554175 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1083 0.35297223480638673 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 1024 0.3337429071484211 No Hit AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA 1012 0.32983185745527555 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 1003 0.32689857018541635 No Hit GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA 977 0.318424629183601 No Hit GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT 953 0.31060252979730985 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 950 0.30962476737402345 No Hit GTACATGGGATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATA 919 0.29952122233339745 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 884 0.2881139940617229 No Hit ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC 881 0.2871362316384365 No Hit TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC 879 0.2864843900229122 No Hit CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT 840 0.2737734785201892 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 840 0.2737734785201892 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 811 0.26432177509508736 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 801 0.2610625670174661 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 786 0.25617375490103417 No Hit GTACATGGGCTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATA 778 0.25356638843893714 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 679 0.22130022847048625 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 667 0.21738917877734068 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 664 0.2164114163540543 No Hit CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT 608 0.19815985111937504 No Hit ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC 607 0.1978339303116129 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 582 0.18968591011755964 No Hit ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT 558 0.18186381073126853 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA 557 0.1815378899235064 No Hit CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT 533 0.17371579053721528 No Hit GGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTA 524 0.17078250326735608 No Hit GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT 524 0.17078250326735608 No Hit GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 518 0.1688269784207833 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 511 0.1665455327664484 No Hit GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT 490 0.1597011958034437 No Hit GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT 486 0.15839751257239518 No Hit AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG 477 0.155464225302536 No Hit ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC 476 0.15513830449477387 No Hit GGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGG 473 0.15416054207148747 No Hit GAGTACATGGGATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTA 470 0.15318277964820107 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 469 0.15285685884043895 No Hit ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC 468 0.1525309380326768 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 467 0.1522050172249147 No Hit CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT 466 0.15187909641715255 No Hit TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA 457 0.14894580914729338 No Hit GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 437 0.1424273929920508 No Hit GTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTAT 437 0.1424273929920508 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 437 0.1424273929920508 No Hit AGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAAT 435 0.14177555137652653 No Hit ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA 433 0.14112370976100228 No Hit GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA 432 0.14079778895324013 No Hit TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT 420 0.1368867392600946 No Hit GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT 419 0.13656081845233245 No Hit AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG 412 0.13427937279799754 No Hit AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA 406 0.13232384795142477 No Hit GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC 401 0.1306942439126141 No Hit CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT 400 0.130368323104852 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 392 0.12776095664275494 No Hit TTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTT 381 0.12417582775737153 No Hit GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC 379 0.12352398614184726 No Hit GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG 369 0.12026477806422596 No Hit TATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTAT 367 0.11961293644870169 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 359 0.11700556998660465 No Hit CTATAGTATAAGTTTGAAATTTCGGTTGGGGTGACCTCGGAGAATAAAAA 353 0.11505004514003186 No Hit AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT 352 0.11472412433226975 No Hit CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC 350 0.11407228271674548 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 341 0.11113899544688631 No Hit GTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 334 0.1088575497925514 No Hit GTACATGGGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC 324 0.10559834171493011 No Hit GAGTACATGGGCTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCA 324 0.10559834171493011 No Hit GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG 323 0.10527242090716799 No Hit AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG 321 0.1046205792916437 No Hit TAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACAAC 319 0.10396873767611946 No Hit CTATTTTGCCACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCT 318 0.10364281686835733 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAA 313 0.10201321282954667 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 3.2592080776212997E-4 0.0 5 0.0 0.0 0.0 3.2592080776212997E-4 0.0 6 0.0 0.0 0.0 3.2592080776212997E-4 0.0 7 0.0 0.0 0.0 6.518416155242599E-4 0.0 8 0.0 0.0 0.0 6.518416155242599E-4 0.0 9 0.0 0.0 0.0 0.0013036832310485199 0.0 10-11 0.0 0.0 0.0 0.005214732924194079 0.0 12-13 0.0 0.0 0.0 0.020695971292895254 0.0 14-15 0.0 0.0 0.0 0.04416226945176861 0.0 16-17 0.0 0.0 0.0 0.12645727341170643 0.0 18-19 0.0 0.0 0.0 0.20255978202416375 0.0 20-21 0.0 0.0 0.0 0.34987598713264656 0.0 22-23 0.0 0.0 0.0 0.7250108368668581 0.0 24-25 0.0 0.0 0.0 1.0998197657933075 0.0 26-27 0.0 0.0 0.0 1.2554469514997246 0.0 28-29 0.0 0.0 0.0 1.3851634329890523 0.0 30-31 0.0 0.0 0.0 1.5363906877906806 0.0 32-33 0.0 0.0 0.0 1.73520238052558 0.0 34-35 0.0 0.0 0.0 1.9970797495624513 0.0 36-37 0.0 0.0 0.0 2.2592830394070846 0.0 38-39 0.0 0.0 0.0 2.589277857266241 0.0 40-41 0.0 0.0 0.0 3.0211229275510636 0.0 42-43 0.0 0.0 0.0 3.3436215668316915 0.0 44-45 0.0 0.0 0.0 3.6661202061123186 0.0 46-47 0.0 0.0 0.0 4.027566381920521 0.0 48-49 0.0 0.0 0.0 4.340124436564404 0.0 50-51 0.0 0.0 0.0 4.675333987347754 0.0 52-53 0.0 0.0 0.0 4.967359031102623 0.0 54-55 0.0 0.0 0.0 5.259384074857492 0.0 56-57 0.0 0.0 0.0 5.633867082976179 0.0 58-59 0.0 0.0 0.0 5.96923959416341 0.0 60-61 0.0 0.0 0.0 6.30444914494676 0.0 62-63 0.0 0.0 0.0 6.6328143587671065 0.0 64-65 0.0 0.0 0.0 6.943253928160535 0.0 66-67 0.0 0.0 0.0 7.326699758492682 0.0 68-69 0.0 0.0 0.0 7.797655325708959 0.0 70-71 0.0 0.0 0.0 8.268936813733 0.0 72-73 0.0 0.0 0.0 8.664604674356225 0.0 74-75 0.0 0.0 0.0 9.09367941777507 0.0 76-77 0.0 0.0 0.0 9.4781030105305 0.0 78-79 0.0 0.0 0.0 10.093278535181522 0.0 80-81 0.0 0.0 0.0 10.764186517959867 0.0 82-83 0.0 0.0 0.0 11.287289414418085 0.0 84-85 0.0 0.0 0.0 11.791162983218339 0.0 86-87 0.0 0.0 0.0 12.30579193867474 0.0 88-89 0.0 0.0 0.0 12.9696926240862 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAAGATC 20 0.002064467 71.32082 3 GATCCTC 20 0.0020711657 71.26261 6 CCTCATC 20 0.0020711657 71.26261 9 TCTAATA 35 3.3712713E-6 67.99124 2 GTTAAGA 25 0.0049561807 57.178093 1 TTAAGAT 25 0.004978706 57.11264 2 AGATCCT 25 0.004998074 57.056656 5 TTGGATA 25 0.004998074 57.056656 3 GCTTTGC 80 6.91216E-11 53.604465 1 AGTACTC 135 0.0 52.83024 5 TAACTTT 550 0.0 50.963566 6 ATCCTAC 160 0.0 47.508408 6 ACTTTAT 600 0.0 47.508408 8 AACTTTA 610 0.0 46.72195 7 TCCTACT 165 0.0 46.061237 7 TATCCTA 175 0.0 43.47174 5 ATACTTG 55 4.9495808E-5 43.189457 6 AGGCTTA 45 9.605632E-4 42.2297 6 CTTTGCG 105 1.0022632E-9 40.794743 2 ATCTATA 690 0.0 40.742847 1 >>END_MODULE