FastQCFastQC Report
Fri 10 Feb 2017
SRR2031588_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031588_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences306823
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT129784.229800243136923No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT67862.211698601473814No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT46481.51487991447838No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35821.1674483334039496No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC34431.1221453411250133No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC33671.0973753597350917No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33221.0827089233857956No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT30460.9927547804434479No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA29800.9712440071311473No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC29020.9458221841257011No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT28610.9324594310074538No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG24480.7978541374016941No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22800.7430994416976563No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC22280.7261515596940256No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22150.7219145891931179No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC21310.694537241341099No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC20980.6837818546849487No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA19850.646952803407828No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC19450.6339159710973428No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT18180.5925240285115523No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC16990.5537394523878588No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16210.5283176293824127No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG13880.4523780811738364No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA13630.4442300609797832No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA12890.4201119212053855No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC12250.3992529895086092No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG12120.3950160190077015No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT12020.3917568109300802No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11650.3796977410428814No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC11570.37709037458078437No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA11130.36274985903925067No Hit
GGTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11090.36144617580820215No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG10230.3334169863406589No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC9930.32363936210779504No Hit
ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC9470.3086470049507371No Hit
CAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGT9000.29332872698591694No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA8910.2903954397160578No Hit
GTACATGGGATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATA8780.2861584692151501No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA8740.2848547859841016No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT8540.27833636982885895No Hit
ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG8410.2740993993279513No Hit
GTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA8270.2695365080192815No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC7830.25519599247774777No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC7740.25226270520788857No Hit
GTACATGGGCTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATA6880.22423351574034542No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT6650.21673733716181645No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT6600.21510773312300577No Hit
GGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTA6390.20826339616000106No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT6340.20663379212119037No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT6140.2001153759659478No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT6030.19653024708056435No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6020.1962043262728022No Hit
ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC6000.19555248465727798No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG5990.19522656384951584No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT5930.19327103900294307RNA PCR Primer, Index 33 (95% over 22bp)
AGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAG5890.19196735577189455No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA5850.190663672540846No Hit
GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT5500.17925644426917148No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA5410.1763231569993123No Hit
ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA5400.17599723619155017No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT5230.17045658245959397No Hit
TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT5100.1662196119586863No Hit
AGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTA5080.16556777034316203No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5010.16328632468882712No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4780.15579014611029812No Hit
CTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCT4570.14894580914729338No Hit
GTATCAACGCAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4560.14861988833953127No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG4410.14373107622309933No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA4370.1424273929920508No Hit
GGTATCAACGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4360.14210147218428867No Hit
GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC4290.13982002652995376No Hit
AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG4230.13786450168338096No Hit
GTACATGGGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC4200.1368867392600946No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC4190.13656081845233245No Hit
AACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACG4100.13362753118247328No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT4030.13134608552813837No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT3980.1297164814893277No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC3950.12873871906604134No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC3910.1274350358349928No Hit
GTTTCTAGCTGTAATTCTTTTAGTTAGAAGTTTTCTAGTTAGTTCATTAT3890.12678319421946857No Hit
GTACATGGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3880.12645727341170643No Hit
GGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGG3860.12580543179618214No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT3830.12482766937289577No Hit
GAGTACATGGGATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTA3800.12384990694960939No Hit
GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT3770.122872144526323No Hit
GGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAG3710.12091661967975022No Hit
AGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTA3700.12059069887198808No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA3690.12026477806422596No Hit
CACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACT3680.11993885725646383No Hit
ACTTAAAATTGGGTTAATCTATAACTTTATAGATGCAACACTGTTAGTAT3620.11798333240989103No Hit
TATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTAT3580.11667964917884252No Hit
GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG3570.11635372837108038No Hit
GAGTACATGGGCTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCA3530.11505004514003186No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC3410.11113899544688631No Hit
TTTCACCTCTACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTT3400.1108130746391242No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT3370.10983531221583778No Hit
TTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTG3270.1065761041382165No Hit
CAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGA3240.10559834171493011No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC3210.1046205792916437No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTC156.6162867E-494.9890756
TAAGATC156.6162867E-494.9890753
GTATTAT353.3935994E-667.915751
CATGTAG407.537479E-659.3681768
GTACCCA504.2064858E-757.0492361
GTTAAGA250.00500065557.0492361
ATCATAA250.00500065557.0492361
GCTCATA250.00500065557.0492361
ATTTAAC250.005020060656.993453
AGATCCT250.005020060656.993455
ACTTGCT504.2350257E-756.993458
ATAATGT250.005020060656.993454
CTTGCTA504.2350257E-756.993459
CTCATAG250.005020060656.993452
TATGGGA903.6379788E-1252.771714
TGGGAGC451.514372E-552.771716
ACCCATG558.177394E-751.812233
GTATATG1300.051.198031
ATGGGAG752.3792381E-950.6608475
TATCCTA1350.049.2535975
Produced by FastQC (version 0.11.5)