FastQCFastQC Report
Fri 10 Feb 2017
SRR2031586_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031586_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1454337
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC67400.4634414169480664No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC58500.40224514675759476No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT52210.35899519849938494No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC44560.30639391007723793No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA39310.27029498665027435No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37070.2548927793214365No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC36930.25393014136338415No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC35160.2417596471794364No Hit
GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT34740.2388717333052793No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA33210.2283514756208499No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT32980.22676999897547817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32430.2229882069974153No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA31740.2182437770613001No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT28800.19802837994220046No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG28730.19754706096317426No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC27510.1891583587572894No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG26410.18159477480116368No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC25750.17705662442748826No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT25650.17636902588602227No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT25180.17313731274113223No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT25040.17217467478307985No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC25020.17203715507478667No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA24940.17148707624161388No Hit
TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC24670.16963056017965575No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT24320.16722396528452485No Hit
CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA23820.16378597257719496No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC23580.16213573607767662No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT22960.15787262512058758No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT22850.15711626672497503No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG22120.1520967973722734No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA21720.14934640320640952No Hit
CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT21680.14907136378982314No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG21430.1473523674361582No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA21360.146871048457132No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC21120.14522081195761366No Hit
ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA20780.14288297691662935No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC20480.14082018129223145No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT20270.1393762243551529No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA20170.13868862581368693No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA20010.13758846814734138No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19970.13731342873075497No Hit
CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC19440.13366915646098532No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC19320.13284403821122615No Hit
ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC18980.13050620317024184No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC18890.12988736448292246No Hit
ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT18790.1291997659414565No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG18310.12589929294241983No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG18100.12445533600534127No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT18080.12431781629704808No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA17910.12314889877655592No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA17780.12225502067265015No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA17690.12163618198533077No Hit
TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG17570.1208110637355716No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG17540.12060478417313181No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG17380.11950462650678625No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG17190.11819818927800091No Hit
ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA17150.11792314986141451No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA17140.11785439000726791No Hit
CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA16970.11668547248677576No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC16860.1159291140911632No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA16780.11537903525799041No Hit
GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT16100.11070336517602179No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA16090.11063460532187519No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA15940.10960320750967624No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT15830.10884684911406366No Hit
ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC15800.10864056955162388No Hit
GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT15520.10671529363551913No Hit
ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG15400.10589017538575997No Hit
GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG15250.10485877757356102No Hit
GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT15080.10368986005306886No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG15010.10320854107404268No Hit
ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT14920.10258970238672331No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT14900.10245218267843009No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT14840.10203962355355052No Hit
TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA14660.10080194617891175No Hit
GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT14620.10052690676232538No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC14590.10032062719988559No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGTA156.583268E-495.1268544
CGGGAAC200.002063801371.3402253
GGCGCAC301.295647E-463.3894778
ATGTATT1250.057.2102931
ACTACGC250.00500395857.0505378
GATCTTC250.00500395857.0505379
ATGGGCG558.1171856E-751.887385
GCGCACA405.365159E-447.542119
GGGCGCA652.5765476E-643.8789757
CTCAATT1201.8189894E-1243.5802659
GACATAT4500.043.4374431
GAACCTC459.5840206E-442.259669
AAAGACA1750.040.7686544
TAACTTT12950.038.5450026
GTCTTAA500.001586981838.1401981
GTTTGCA250.001622325937.97085684-85
ACATATA5400.036.1553762
TGGGTAT2400.035.654126
TACCTGG1900.035.0878872
GGGCCTA958.651223E-735.02627
Produced by FastQC (version 0.11.5)