##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031586_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1454337 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.16935208277036 34.0 31.0 34.0 30.0 34.0 2 32.397319190806535 34.0 31.0 34.0 31.0 34.0 3 32.51540805191644 34.0 31.0 34.0 31.0 34.0 4 35.654165437584275 37.0 35.0 37.0 35.0 37.0 5 35.46365663529154 37.0 35.0 37.0 33.0 37.0 6 35.67636043090425 37.0 36.0 37.0 33.0 37.0 7 35.77198751046009 37.0 36.0 37.0 35.0 37.0 8 35.82921221147506 37.0 37.0 37.0 35.0 37.0 9 37.740080875340446 39.0 39.0 39.0 35.0 39.0 10-11 37.69240004208103 39.0 38.0 39.0 35.0 39.0 12-13 37.76576336846274 39.0 38.5 39.0 35.0 39.0 14-15 39.19959576081747 41.0 39.0 41.0 36.0 41.0 16-17 39.16072203347642 41.0 39.0 41.0 36.0 41.0 18-19 39.15133734478322 41.0 39.0 41.0 36.0 41.0 20-21 39.186013970627165 41.0 39.0 41.0 36.0 41.0 22-23 39.108756086106595 41.0 39.0 41.0 36.0 41.0 24-25 39.04335102524381 41.0 39.0 41.0 35.5 41.0 26-27 38.95966271916344 41.0 39.0 41.0 35.0 41.0 28-29 38.86624042433081 41.0 39.0 41.0 35.0 41.0 30-31 38.725788452057536 40.0 38.0 41.0 35.0 41.0 32-33 38.582926790695694 40.0 38.0 41.0 35.0 41.0 34-35 38.51693933386829 40.0 38.0 41.0 35.0 41.0 36-37 38.384504072989955 40.0 38.0 41.0 34.0 41.0 38-39 38.21524069043144 40.0 38.0 41.0 34.0 41.0 40-41 38.14119939188785 40.0 38.0 41.0 34.0 41.0 42-43 38.01947966667973 40.0 38.0 41.0 33.0 41.0 44-45 37.81093377944727 40.0 38.0 41.0 33.0 41.0 46-47 37.6091765526147 40.0 38.0 41.0 33.0 41.0 48-49 37.717012631872805 40.0 38.0 41.0 33.0 41.0 50-51 37.5034806238169 39.5 37.5 40.5 33.0 41.0 52-53 37.68388172755008 40.0 38.0 41.0 33.0 41.0 54-55 38.00829897059622 40.0 38.0 41.0 33.0 41.0 56-57 37.95443800164611 40.0 38.0 41.0 33.0 41.0 58-59 37.83260585407646 40.0 38.0 41.0 33.0 41.0 60-61 37.63794326899473 40.0 38.0 41.0 32.5 41.0 62-63 37.349837073525606 40.0 37.5 41.0 31.5 41.0 64-65 37.13787072734861 40.0 37.0 41.0 31.0 41.0 66-67 36.953065211158076 39.0 36.5 41.0 31.0 41.0 68-69 36.69910103366689 39.0 36.0 41.0 31.0 41.0 70-71 36.3834816139588 39.0 36.0 40.5 31.0 41.0 72-73 36.00860323295082 37.5 35.0 40.0 30.5 41.0 74-75 35.51774141756691 37.0 35.0 39.0 30.0 41.0 76-77 35.074345904697466 36.5 35.0 39.0 29.5 41.0 78-79 34.660559416421364 36.0 35.0 38.5 29.0 40.0 80-81 34.255628509760804 36.0 34.5 37.0 29.0 39.0 82-83 33.86520146293466 35.0 34.0 37.0 29.0 39.0 84-85 33.48317583888741 35.0 34.0 36.5 28.5 38.0 86-87 33.20560846626332 35.0 34.0 36.0 28.0 37.0 88-89 32.91836520696373 35.0 34.0 36.0 26.5 37.0 90-91 32.71789378940369 35.0 34.0 36.0 26.0 36.5 92-93 32.53115715270945 35.0 34.0 35.0 26.0 36.0 94-95 32.34565131740443 35.0 34.0 35.0 26.0 36.0 96-97 32.23586933427397 35.0 34.0 35.0 25.0 36.0 98-99 32.1391771645774 35.0 34.0 35.0 25.0 35.5 100-101 31.711558600241897 34.5 32.5 35.0 24.0 35.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2609183792583707 1101 2 0.18265767906508756 1101 3 0.3383371048795709 1101 4 0.27452422197427495 1101 5 0.30625164866365395 1101 6 0.2871678334695247 1101 7 0.31758545330717425 1101 8 0.33094152898069495 1101 9 0.24814495720240615 1101 10-11 0.2879795618555434 1101 12-13 0.24120169161295024 1101 14-15 0.31315573738940117 1101 16-17 0.3119421998310301 1101 18-19 0.3595153482601461 1101 20-21 0.14701484297876277 1101 22-23 0.27228562391147904 1101 24-25 0.2899386333388705 1101 26-27 0.15208513743243657 1101 28-29 0.23047908783877347 1101 30-31 0.25655007011900466 1101 32-33 0.3797541784457579 1101 34-35 0.3428955428854934 1101 36-37 0.2728287116966115 1101 38-39 0.34266292236866747 1101 40-41 0.34292436391830705 1101 42-43 0.2523119325892793 1101 44-45 0.4273317757470636 1101 46-47 0.5801800797632666 1101 48-49 0.547755910990503 1101 50-51 0.5902444790791463 1101 52-53 0.6147046586442997 1101 54-55 0.5540624074753069 1101 56-57 0.5958493053400957 1101 58-59 0.5688310518216255 1101 60-61 0.4515645164393831 1101 62-63 0.31530312011719985 1101 64-65 0.5833695269328913 1101 66-67 0.5098179029442065 1101 68-69 0.47076999822157006 1101 70-71 0.5369961939377248 1101 72-73 0.5638956586916635 1101 74-75 0.4516069693644056 1101 76-77 0.4390983380389528 1101 78-79 0.4937645843887637 1101 80-81 0.534708889444218 1101 82-83 0.540896166868734 1101 84-85 0.43931414341093955 1101 86-87 0.5427700868544179 1101 88-89 0.5854316485926603 1101 90-91 0.4175244619380152 1101 92-93 0.48073166994065275 1101 94-95 0.37538033452472774 1101 96-97 0.5556347318354113 1101 98-99 0.6022264294305373 1101 100-101 0.7196106928602255 1102 1 0.0025989069160843314 1102 2 0.08607007280548373 1102 3 0.09350116337774494 1102 4 -0.04183299447013411 1102 5 0.11149800979379165 1102 6 0.2308970060115243 1102 7 0.17301771478781802 1102 8 0.24942739076982434 1102 9 0.2554011140723773 1102 10-11 0.10816773560043202 1102 12-13 0.30905028354728614 1102 14-15 0.29726859559290375 1102 16-17 0.2740988165597571 1102 18-19 0.43877551666398773 1102 20-21 0.2715378618162845 1102 22-23 0.15119284064365246 1102 24-25 0.2453808229342087 1102 26-27 0.30940474790501327 1102 28-29 0.1628031587686749 1102 30-31 0.3512542123011144 1102 32-33 0.3008760266290551 1102 34-35 0.18058813392198658 1102 36-37 0.10243420582894913 1102 38-39 0.16925616894850748 1102 40-41 0.3221343162058048 1102 42-43 0.4165107193448847 1102 44-45 0.18383900744336046 1102 46-47 -0.2902472320485998 1102 48-49 0.3031178123539604 1102 50-51 0.20324225172819155 1102 52-53 0.22119519959704803 1102 54-55 0.26753485911530817 1102 56-57 0.30655195686614434 1102 58-59 0.22901974355510646 1102 60-61 0.10409201300051052 1102 62-63 -0.1441980856451366 1102 64-65 0.014891434972646778 1102 66-67 0.25036173341229073 1102 68-69 0.3912515549499531 1102 70-71 0.3112028080765157 1102 72-73 0.1391586817881887 1102 74-75 0.21349552699638963 1102 76-77 0.2098488171784041 1102 78-79 0.308821073922509 1102 80-81 0.3019996364413231 1102 82-83 0.057243742969895095 1102 84-85 0.18111409621642593 1102 86-87 0.11005608562275881 1102 88-89 0.025860802141743022 1102 90-91 0.0936542526130637 1102 92-93 0.15532548055251283 1102 94-95 0.2948662020692865 1102 96-97 0.1140609673820876 1102 98-99 0.016100753887080543 1102 100-101 0.15435060503238418 1103 1 -0.032178290268532805 1103 2 -0.047503919650289106 1103 3 -0.08402129276308301 1103 4 0.01641255690326915 1103 5 -0.08499202961777286 1103 6 -0.05865258971659415 1103 7 -0.04924252697099263 1103 8 -0.019441016040424586 1103 9 0.1294463276323512 1103 10-11 4.35175320909309E-4 1103 12-13 0.04235932830415834 1103 14-15 0.10587200017423726 1103 16-17 0.16337732996255028 1103 18-19 0.30847980040545764 1103 20-21 0.1347431904891181 1103 22-23 0.11109249178790037 1103 24-25 0.28685157917005455 1103 26-27 0.09813946075678359 1103 28-29 0.15973550474524245 1103 30-31 0.3198796879050718 1103 32-33 0.33201675359591576 1103 34-35 0.28683952273324564 1103 36-37 0.43989693797343676 1103 38-39 0.2143613505266302 1103 40-41 0.12501786327406705 1103 42-43 0.19887530428287903 1103 44-45 0.2835300949217725 1103 46-47 0.6279199187991367 1103 48-49 0.27043456765461826 1103 50-51 0.43944752178126834 1103 52-53 0.44239006123396507 1103 54-55 0.4388984911413374 1103 56-57 0.5324220854615476 1103 58-59 0.4445353043758402 1103 60-61 0.5160260425912213 1103 62-63 0.6562230905399744 1103 64-65 0.7331283842365579 1103 66-67 0.46737789201829116 1103 68-69 0.4336943086610603 1103 70-71 0.5414186378665349 1103 72-73 0.6507432421604165 1103 74-75 0.5398382263138046 1103 76-77 0.08531351512984742 1103 78-79 0.3541528958656599 1103 80-81 0.46988868422460683 1103 82-83 0.3204454590293935 1103 84-85 0.32797671193933553 1103 86-87 0.3665417490008096 1103 88-89 0.465769069027985 1103 90-91 0.4812931166983745 1103 92-93 0.37820463795475945 1103 94-95 0.1870020881342782 1103 96-97 0.28210883382718066 1103 98-99 0.374461772562249 1103 100-101 0.33478564547320033 1104 1 0.19081575001784046 1104 2 0.2066071703218526 1104 3 0.2495305233725773 1104 4 0.12371647701567667 1104 5 -0.005634103590530515 1104 6 0.11360140064951452 1104 7 0.17517870955739312 1104 8 0.16405293480915617 1104 9 0.3685415180821465 1104 10-11 0.41857933983909135 1104 12-13 0.34709480083063227 1104 14-15 0.43087549231739075 1104 16-17 0.3163038091300976 1104 18-19 0.4978083165827769 1104 20-21 0.29077783507743504 1104 22-23 0.3143153132752303 1104 24-25 0.27832379719608724 1104 26-27 0.29791185519135155 1104 28-29 0.3557325922030543 1104 30-31 0.4585016534538937 1104 32-33 0.4695120684355345 1104 34-35 0.33716367578582407 1104 36-37 0.35015454752718256 1104 38-39 0.38825301381703525 1104 40-41 0.4015472481575486 1104 42-43 0.36421042677335436 1104 44-45 0.3677305647967444 1104 46-47 0.6005661669100633 1104 48-49 0.49500465480966227 1104 50-51 0.47023684726354276 1104 52-53 0.4636023816414152 1104 54-55 0.5337943999929209 1104 56-57 0.4029021303126754 1104 58-59 0.2929886167921296 1104 60-61 0.4515111470995308 1104 62-63 0.5708251789995913 1104 64-65 0.5439689446733951 1104 66-67 0.35059714867532676 1104 68-69 0.26178700436171454 1104 70-71 0.22941034268341554 1104 72-73 0.385800503827177 1104 74-75 0.2955800365270491 1104 76-77 0.2496521917055574 1104 78-79 0.3161033889689193 1104 80-81 0.3215250614215748 1104 82-83 0.3770706392460923 1104 84-85 0.42101846659431175 1104 86-87 0.4488757394680505 1104 88-89 0.4537071117718199 1104 90-91 0.3956727192956464 1104 92-93 0.3539754851012802 1104 94-95 0.22096815777614154 1104 96-97 0.2886519407790473 1104 98-99 0.5246814457482074 1104 100-101 0.4344473026262783 1105 1 -0.006022980201649375 1105 2 0.13216727820540797 1105 3 0.12181455011835851 1105 4 0.11229191936291727 1105 5 -0.1252991373176826 1105 6 -0.023545054102172003 1105 7 -0.012838054405499122 1105 8 0.01895304742360082 1105 9 0.17148911534978595 1105 10-11 0.12875725380550307 1105 12-13 0.1839868348184055 1105 14-15 0.18718229233977723 1105 16-17 0.24884167882117225 1105 18-19 0.15701480307837556 1105 20-21 0.11374233353989638 1105 22-23 0.05482509375832478 1105 24-25 0.11711962317878744 1105 26-27 0.09477700857511451 1105 28-29 0.12152253948312364 1105 30-31 0.1528729175705692 1105 32-33 0.18269132883973072 1105 34-35 0.22942548059025825 1105 36-37 0.3644287742283083 1105 38-39 0.42267655848150554 1105 40-41 0.3856804088675645 1105 42-43 0.2768060301754929 1105 44-45 0.5583189225940757 1105 46-47 0.49266756685624813 1105 48-49 0.543830930630179 1105 50-51 0.39737361753429923 1105 52-53 0.2731407226157927 1105 54-55 0.3254056171377613 1105 56-57 0.3513730476779884 1105 58-59 0.2950732780549927 1105 60-61 0.3641344376441893 1105 62-63 0.3380684003143628 1105 64-65 0.3033845292843935 1105 66-67 0.21501381368678096 1105 68-69 0.24635413093589165 1105 70-71 0.335607688208718 1105 72-73 0.3011807716822261 1105 74-75 0.28522504522743475 1105 76-77 0.3635995295567156 1105 78-79 0.31840653789986817 1105 80-81 0.21654474637801258 1105 82-83 0.08594755846163338 1105 84-85 0.1814175466589134 1105 86-87 0.3275359345661002 1105 88-89 0.2775750537323418 1105 90-91 0.4724305067558987 1105 92-93 0.2659878528274646 1105 94-95 0.14159062492926466 1105 96-97 0.3045226923826405 1105 98-99 0.07866866512247128 1105 100-101 0.2526523211014329 1106 1 0.11877880319304523 1106 2 0.1349290820149207 1106 3 0.08113341897364279 1106 4 0.2252613022585095 1106 5 0.0808802706867695 1106 6 -0.0370024467067509 1106 7 0.10779883325667328 1106 8 0.21889876722512014 1106 9 0.20224960141360526 1106 10-11 0.2138329303023525 1106 12-13 0.23057904543923513 1106 14-15 0.2977176009261271 1106 16-17 0.34561405532091527 1106 18-19 0.31672489902459944 1106 20-21 0.29614684391714263 1106 22-23 0.3014270338486895 1106 24-25 0.2784009431093537 1106 26-27 0.1674040848353755 1106 28-29 0.26317252096298205 1106 30-31 0.33134467394465617 1106 32-33 0.3852204788425482 1106 34-35 0.3731783930301589 1106 36-37 0.41848877945149354 1106 38-39 0.3563608354880401 1106 40-41 0.4090780761845636 1106 42-43 0.35981888843760856 1106 44-45 0.4924717503705196 1106 46-47 0.6718203860980694 1106 48-49 0.4901071895296596 1106 50-51 0.43050843555054286 1106 52-53 0.32257069518551873 1106 54-55 0.3807057029960603 1106 56-57 0.2586196248676771 1106 58-59 0.3317687405376404 1106 60-61 0.3749341488325726 1106 62-63 0.42625836052594934 1106 64-65 0.6203023698883712 1106 66-67 0.4830035175183127 1106 68-69 0.5263657038838758 1106 70-71 0.484669989245333 1106 72-73 0.4948117243679846 1106 74-75 0.4230233516169619 1106 76-77 0.46237583539661387 1106 78-79 0.36349409459109694 1106 80-81 0.3284601034068686 1106 82-83 0.40713356372921083 1106 84-85 0.29042430437292666 1106 86-87 0.23805127213602617 1106 88-89 0.38150200419227787 1106 90-91 0.45651599636157414 1106 92-93 0.30959635945630026 1106 94-95 0.23969525598607078 1106 96-97 0.36469007339719894 1106 98-99 0.30171675430586475 1106 100-101 0.5010687219146099 1107 1 0.076292727708406 1107 2 0.0060135920281751964 1107 3 0.0992582348029103 1107 4 0.2554387341576074 1107 5 0.15393023469799516 1107 6 -0.026667952393296446 1107 7 0.10600986111858646 1107 8 0.1775785025667389 1107 9 0.21199958007797903 1107 10-11 0.27943877352656443 1107 12-13 0.07396588691310768 1107 14-15 0.23048944498443547 1107 16-17 0.21092747969698422 1107 18-19 0.2210945963163411 1107 20-21 0.1594788729593546 1107 22-23 0.11465576625484886 1107 24-25 0.21784399469969884 1107 26-27 0.1633994280330171 1107 28-29 0.14504239135509778 1107 30-31 0.15381213510173808 1107 32-33 0.11610416022838166 1107 34-35 0.3020043538982051 1107 36-37 0.2617790067684993 1107 38-39 0.37547836177916594 1107 40-41 0.2607745402674482 1107 42-43 0.20423386556420553 1107 44-45 0.015053699303493318 1107 46-47 0.2949849183969846 1107 48-49 0.2870773695701416 1107 50-51 0.2638957117881304 1107 52-53 0.23004573250273808 1107 54-55 0.32735617068909306 1107 56-57 0.30243897571715195 1107 58-59 0.33432418498691874 1107 60-61 0.39870686724899684 1107 62-63 0.2187408129797106 1107 64-65 0.29586156457717294 1107 66-67 0.12191052156161675 1107 68-69 0.20791497629875977 1107 70-71 0.2879482686116077 1107 72-73 0.3794729387757343 1107 74-75 0.25563046853780236 1107 76-77 0.28475473357656256 1107 78-79 0.3854641538638788 1107 80-81 0.32729107210871433 1107 82-83 0.4012336592161958 1107 84-85 0.2577767705297944 1107 86-87 0.36304422735374686 1107 88-89 0.4477897823404078 1107 90-91 0.3291302305253083 1107 92-93 0.17634289382598212 1107 94-95 0.27122528608138197 1107 96-97 0.29280511509426077 1107 98-99 0.1776110390954102 1107 100-101 0.2755197522240813 1108 1 0.01570421663590338 1108 2 0.05626500998869943 1108 3 -0.006240786183823843 1108 4 0.10454659108378195 1108 5 0.18808538761710736 1108 6 0.18568599997058044 1108 7 0.05948145134868099 1108 8 0.08636939820300427 1108 9 0.08145068916671505 1108 10-11 0.12687817122656497 1108 12-13 0.04711426487657633 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-1.3906287194423683 2316 84-85 -1.8786219955685581 2316 86-87 -1.3181267799550511 2316 88-89 -1.2338709213566332 2316 90-91 -1.3539062868339258 2316 92-93 -1.4525238754437346 2316 94-95 -1.3526679001431603 2316 96-97 -1.2632734495290023 2316 98-99 -0.9323807659854388 2316 100-101 -1.3870061483842058 >>END_MODULE >>Per sequence quality scores pass #Quality Count 5 1.0 6 3.0 7 97.0 8 435.0 9 506.0 10 548.0 11 633.0 12 859.0 13 1034.0 14 1310.0 15 1748.0 16 2378.0 17 3618.0 18 4517.0 19 5883.0 20 7332.0 21 9028.0 22 9069.0 23 7799.0 24 7069.0 25 7260.0 26 8445.0 27 9511.0 28 11047.0 29 13417.0 30 16386.0 31 20646.0 32 25777.0 33 34019.0 34 47462.0 35 70091.0 36 115880.0 37 262191.0 38 545508.0 39 202783.0 40 47.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 36.176793533804556 26.723292635146013 15.228409909913024 21.871503921136405 2 12.51138041402274 24.06580519492173 43.805154887521134 19.617659503534398 3 13.439730337202525 33.489906249181765 33.44222466295596 19.628138750659758 4 9.062743822250651 20.846854124335216 47.313647429234685 22.77675462417945 5 8.614855781361815 42.96721282090525 39.17660457798864 9.241326819744282 6 22.39599913456066 47.16327450275135 20.367797939207023 10.072928423480967 7 22.106304610538412 35.03011545982056 29.807139796088507 13.056440133552522 8 21.360705638854785 39.73276725685789 24.01119199886454 14.895335105422786 9 24.944692513801897 16.991024719742104 26.192426646530215 31.871856119925784 10-11 19.849691102916424 29.745319686344825 34.23580918425743 16.16918002648132 12-13 20.714089352287893 29.153693569179023 32.14104854550879 17.9911685330243 14-15 20.186220380881835 30.927639342285463 28.78364154730015 20.102498729532552 16-17 17.74547226508568 33.453136894036476 30.430907391526386 18.37048344935145 18-19 17.32205847554164 32.95364495341658 32.70573198985235 17.018564581189434 20-21 17.96819293544592 32.67254840885603 32.33060424346314 17.028654412234904 22-23 18.9429272582627 31.413764485122776 31.518760782404627 18.124547474209898 24-25 18.727640154929702 32.27346894151768 30.953520401392527 18.04537050216009 26-27 18.096802873061748 32.12278172115541 31.90519116270851 17.875224243074335 28-29 18.174432748393254 30.034201151452518 33.581453267021324 18.2099128331329 30-31 18.949768863750286 31.80600507310204 31.467775350554927 17.776450712592748 32-33 17.80887098382287 32.35161451575529 31.811127682235963 18.028386818185883 34-35 17.971728698369084 32.02428323009041 32.11164262478366 17.89234544675684 36-37 18.889844650861527 30.50249013811792 31.2343700256543 19.373295185366253 38-39 18.048774114940347 32.70699982191198 31.86311013197079 17.38111593117689 40-41 17.188691479347636 31.452304383371942 33.84167493503913 17.517329202241296 42-43 17.96815318595346 31.304195657540173 31.313546997704105 19.41410415880226 44-45 18.27500999322079 31.607395362112662 31.651534239132868 18.46606040553368 46-47 18.37958145848694 30.992365097193414 31.993894834037334 18.634158610282313 48-49 17.547096119269447 31.399592226494953 31.891604511317006 19.16170714291859 50-51 17.903052183219753 30.66869780878159 30.8325240071434 20.595726000855255 52-53 17.858861926465515 30.96970107756033 32.00910519382082 19.162331802153332 54-55 18.69585967481482 30.968154176850284 31.332132002349443 19.003854145985457 56-57 18.730649888395355 31.468586346888234 30.387028046583108 19.413735718133303 58-59 17.83499329294186 30.699881000476964 31.262599392820245 20.20252631376093 60-61 19.215551391881387 32.29883015445762 30.00820110683956 18.477417346821433 62-63 18.174530360044088 31.791693750564363 31.867689501695345 18.1660863876962 64-65 18.23052220780902 30.856729543119805 31.26802224223546 19.644726006835715 66-67 18.496852798237335 31.056612462086104 30.671596921389995 19.77493781828657 68-69 18.107206075752096 31.438423845385792 30.92050081037333 19.533869268488782 70-71 18.422624721520023 30.208461661815704 32.412866824457645 18.95604679220662 72-73 18.665021603496196 30.764837394038274 30.664922514359322 19.905218488106208 74-75 17.905292697764782 31.117154504881828 30.426823579151186 20.550729218202207 76-77 17.44088654081567 31.326265914698286 32.17965516445375 19.053192380032296 78-79 17.735420196289795 31.211881361283183 31.070243129564552 19.982455312862466 80-81 18.139922674275365 31.60358660329756 30.13800398327959 20.11848673914749 82-83 18.62358710572563 31.068687816220425 30.699211253395127 19.608513824658818 84-85 17.788847048361486 30.423692124077085 30.999104531648026 20.788356295913403 86-87 17.180085043896455 31.52892879027311 31.98273937131924 19.3082467945112 88-89 18.65627700383745 30.005950092864808 31.288334479876195 20.04943842342155 90-91 18.07151282802356 30.327914276302725 32.266170001042944 19.334402894630774 92-93 18.84331718363716 31.416284389847625 30.77813664716053 18.962261779354677 94-95 18.257273695581976 29.61757543251094 31.486665780771155 20.638485091135927 96-97 19.03579457529063 29.51222626022927 31.064670015371647 20.38730914910845 98-99 18.154639327786146 30.268306806815588 31.049943623193045 20.527110242205225 100-101 18.17726767323708 29.954230953416307 31.393767923687065 20.474733449659553 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 511.0 1 320.5 2 253.5 3 463.5 4 380.0 5 274.0 6 463.5 7 818.0 8 1182.5 9 1241.0 10 970.0 11 750.0 12 765.5 13 808.5 14 827.5 15 874.0 16 904.0 17 912.5 18 969.5 19 1076.5 20 1206.5 21 1430.0 22 1735.0 23 2079.0 24 2404.5 25 2971.5 26 4587.0 27 7128.5 28 8846.0 29 14120.5 30 23997.5 31 33469.0 32 54157.5 33 78290.5 34 95051.5 35 108698.5 36 131505.5 37 146528.0 38 132322.5 39 114737.0 40 92964.0 41 70966.0 42 56246.5 43 48048.5 44 44604.5 45 39624.0 46 31542.0 47 22010.0 48 18795.0 49 15145.0 50 10838.5 51 7124.0 52 3384.5 53 2163.0 54 1678.5 55 1467.0 56 1218.5 57 1059.0 58 1046.5 59 900.5 60 741.5 61 715.5 62 660.0 63 468.5 64 330.5 65 234.5 66 140.0 67 115.0 68 82.5 69 44.5 70 35.5 71 29.5 72 22.0 73 18.5 74 18.0 75 16.5 76 11.5 77 10.5 78 7.5 79 4.5 80 6.5 81 6.0 82 3.5 83 2.0 84 2.5 85 1.5 86 1.0 87 2.0 88 1.0 89 0.0 90 0.0 91 0.5 92 1.0 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49369575277256916 2 0.38340494672142705 3 0.20923623616809586 4 0.23962809170089186 5 0.26341900123561457 6 0.2097863150012686 7 0.20098505367050415 8 0.2023602507534361 9 0.19981613615001198 10-11 0.21490892413519014 12-13 0.20187893177440994 14-15 0.21312116792737862 16-17 0.09647007536767613 18-19 0.041324672342105025 20-21 4.1255912487958427E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.144567593343221 46-47 0.21363686683347807 48-49 0.09107242681716823 50-51 0.06652515888683297 52-53 0.07567021948833041 54-55 0.0843339611108017 56-57 0.11445077722701134 58-59 0.09557619726377037 60-61 0.14398313458297493 62-63 0.24739795521945737 64-65 0.13511311339806387 66-67 0.10578703560454007 68-69 0.11080650495724167 70-71 0.09351340163937244 72-73 0.07563583956125712 74-75 0.1418171991773571 76-77 0.21477140442689693 78-79 0.14064828165686494 80-81 0.0868436957871525 82-83 0.0918287852127808 84-85 0.10080194617891175 86-87 0.07477634138442465 88-89 0.09753585310694839 90-91 0.1180262896426344 92-93 0.165058029878907 94-95 0.13380667616927852 96-97 0.09196630492107401 98-99 0.08034588957029905 100-101 0.06552814100170731 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1454337.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 10.419316010977557 #Duplication Level Percentage of deduplicated Percentage of total 1 73.87805672520336 7.697588192968195 2 9.107390414201259 1.897855575218214 3 3.518398199589185 1.0997790808192263 4 1.8646649760653622 0.7771413456090764 5 1.2140071584851708 0.6324562111922954 6 0.9133532367321543 0.5709909601896906 7 0.6493173371006918 0.4735809768660985 8 0.5545297144365288 0.46222562657530697 9 0.4744933938362364 0.44495069539508797 >10 4.857537450732432 11.390978944008427 >50 1.1979762624365864 8.94528896098504 >100 1.4574364343348656 32.23717065327744 >500 0.22027087510114335 15.7229318349517 >1k 0.0905407891520037 16.393149137595902 >5k 0.002027032592955307 1.2539118043483068 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 6740 0.4634414169480664 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 5850 0.40224514675759476 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 5221 0.35899519849938494 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 4456 0.30639391007723793 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 3931 0.27029498665027435 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3707 0.2548927793214365 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 3693 0.25393014136338415 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 3516 0.2417596471794364 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 3474 0.2388717333052793 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 3321 0.2283514756208499 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 3298 0.22676999897547817 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3243 0.2229882069974153 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 3174 0.2182437770613001 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 2880 0.19802837994220046 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 2873 0.19754706096317426 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 2751 0.1891583587572894 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2641 0.18159477480116368 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 2575 0.17705662442748826 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2565 0.17636902588602227 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 2518 0.17313731274113223 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 2504 0.17217467478307985 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 2502 0.17203715507478667 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 2494 0.17148707624161388 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 2467 0.16963056017965575 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 2432 0.16722396528452485 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 2382 0.16378597257719496 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2358 0.16213573607767662 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 2296 0.15787262512058758 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 2285 0.15711626672497503 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 2212 0.1520967973722734 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 2172 0.14934640320640952 No Hit CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT 2168 0.14907136378982314 No Hit CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG 2143 0.1473523674361582 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 2136 0.146871048457132 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 2112 0.14522081195761366 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 2078 0.14288297691662935 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 2048 0.14082018129223145 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 2027 0.1393762243551529 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 2017 0.13868862581368693 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 2001 0.13758846814734138 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1997 0.13731342873075497 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 1944 0.13366915646098532 No Hit ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC 1932 0.13284403821122615 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1898 0.13050620317024184 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 1889 0.12988736448292246 No Hit ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT 1879 0.1291997659414565 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1831 0.12589929294241983 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1810 0.12445533600534127 No Hit CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT 1808 0.12431781629704808 No Hit ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA 1791 0.12314889877655592 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 1778 0.12225502067265015 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1769 0.12163618198533077 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1757 0.1208110637355716 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 1754 0.12060478417313181 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1738 0.11950462650678625 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 1719 0.11819818927800091 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 1715 0.11792314986141451 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1714 0.11785439000726791 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 1697 0.11668547248677576 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 1686 0.1159291140911632 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 1678 0.11537903525799041 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 1610 0.11070336517602179 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 1609 0.11063460532187519 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 1594 0.10960320750967624 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 1583 0.10884684911406366 No Hit ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC 1580 0.10864056955162388 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 1552 0.10671529363551913 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 1540 0.10589017538575997 No Hit GTACATGGGATGACCCACCAAACTCATGCATATCACATAGTTAATCCAAG 1525 0.10485877757356102 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 1508 0.10368986005306886 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 1501 0.10320854107404268 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 1492 0.10258970238672331 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 1490 0.10245218267843009 No Hit GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT 1484 0.10203962355355052 No Hit TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA 1466 0.10080194617891175 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1462 0.10052690676232538 No Hit CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC 1459 0.10032062719988559 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 1.3751970829319478E-4 0.0 8 0.0 0.0 0.0 1.3751970829319478E-4 0.0 9 0.0 0.0 0.0 1.3751970829319478E-4 0.0 10-11 0.0 0.0 0.0 5.156989060994804E-4 0.0 12-13 0.0 0.0 0.0 0.001547096718298441 0.0 14-15 0.0 0.0 0.0 0.0025441146034241033 0.0 16-17 0.0 0.0 0.0 0.007151024831246128 0.0 18-19 0.0 0.0 0.0 0.011551655496628361 0.0 20-21 0.0 0.0 0.0 0.021453074493738386 0.0 22-23 0.0 0.0 0.0 0.045450263590900875 0.0 24-25 0.0 0.0 0.0 0.07071950998977541 0.0 26-27 0.0 0.0 0.0 0.08196174614274408 0.0 28-29 0.0 0.0 0.0 0.09131308630668133 0.0 30-31 0.0 0.0 0.0 0.10874370933284377 0.0 32-33 0.0 0.0 0.0 0.13851672617832045 0.0 34-35 0.0 0.0 0.0 0.18369195035263491 0.0 36-37 0.0 0.0 0.0 0.24564457893871916 0.0 38-39 0.0 0.0 0.0 0.3402581382444372 0.0 40-41 0.0 0.0 0.0 0.45089274356631237 0.0 42-43 0.0 0.0 0.0 0.5472596791527686 0.0 44-45 0.0 0.0 0.0 0.6620886355775862 0.0 46-47 0.0 0.0 0.0 0.8033213759946973 0.0 48-49 0.0 0.0 0.0 0.9533897576696461 0.0 50-51 0.0 0.0 0.0 1.1120531211129197 0.0 52-53 0.0 0.0 0.0 1.2686880688588684 0.0 54-55 0.0 0.0 0.0 1.4222288231682203 0.0 56-57 0.0 0.0 0.0 1.5952629961281326 0.0 58-59 0.0 0.0 0.0 1.799032823891574 0.0 60-61 0.0 0.0 0.0 2.0254934035233925 0.0 62-63 0.0 0.0 0.0 2.2365173958992997 0.0 64-65 0.0 0.0 0.0 2.4553800116479194 0.0 66-67 0.0 0.0 0.0 2.701196490222005 0.0 68-69 0.0 0.0 0.0 2.974241871038143 0.0 70-71 0.0 0.0 0.0 3.305629988097669 0.0 72-73 0.0 0.0 0.0 3.6280793241181373 0.0 74-75 0.0 0.0 0.0 3.9497723017429935 0.0 76-77 0.0 0.0 0.0 4.308079901700912 0.0 78-79 0.0 0.0 0.0 4.73139994375444 0.0 80-81 0.0 0.0 0.0 5.210277947958417 0.0 82-83 0.0 0.0 0.0 5.692078245963625 0.0 84-85 0.0 0.0 0.0 6.133585269438926 0.0 86-87 0.0 0.0 0.0 6.607684463779716 0.0 88-89 0.0 0.0 0.0 7.1302937352209295 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGCGTA 15 6.583268E-4 95.126854 4 CGGGAAC 20 0.0020638013 71.340225 3 GGCGCAC 30 1.295647E-4 63.389477 8 ATGTATT 125 0.0 57.210293 1 ACTACGC 25 0.005003958 57.050537 8 GATCTTC 25 0.005003958 57.050537 9 ATGGGCG 55 8.1171856E-7 51.88738 5 GCGCACA 40 5.365159E-4 47.54211 9 GGGCGCA 65 2.5765476E-6 43.878975 7 CTCAATT 120 1.8189894E-12 43.580265 9 GACATAT 450 0.0 43.437443 1 GAACCTC 45 9.5840206E-4 42.25966 9 AAAGACA 175 0.0 40.768654 4 TAACTTT 1295 0.0 38.545002 6 GTCTTAA 50 0.0015869818 38.140198 1 GTTTGCA 25 0.0016223259 37.970856 84-85 ACATATA 540 0.0 36.155376 2 TGGGTAT 240 0.0 35.65412 6 TACCTGG 190 0.0 35.087887 2 GGGCCTA 95 8.651223E-7 35.0262 7 >>END_MODULE