##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031586_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1454337 Sequences flagged as poor quality 0 Sequence length 101 %GC 37 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.564105155820144 34.0 31.0 34.0 31.0 34.0 2 32.767049865333824 34.0 31.0 34.0 31.0 34.0 3 32.85709983311983 34.0 31.0 34.0 31.0 34.0 4 36.30026671947424 37.0 37.0 37.0 35.0 37.0 5 36.0668180758655 37.0 35.0 37.0 35.0 37.0 6 36.125883478175965 37.0 37.0 37.0 35.0 37.0 7 36.06571723060061 37.0 37.0 37.0 35.0 37.0 8 36.17232938445491 37.0 37.0 37.0 35.0 37.0 9 38.053204999941556 39.0 39.0 39.0 35.0 39.0 10-11 38.019341459372896 39.0 39.0 39.0 35.0 39.0 12-13 38.06419866922178 39.0 39.0 39.0 36.0 39.0 14-15 39.51451967460086 41.0 39.5 41.0 37.0 41.0 16-17 39.47558750138379 41.0 39.0 41.0 36.5 41.0 18-19 39.542698150428684 41.0 39.5 41.0 37.0 41.0 20-21 39.58088702962243 41.0 40.0 41.0 37.0 41.0 22-23 39.49291498462874 41.0 39.5 41.0 36.5 41.0 24-25 39.44105974062408 41.0 39.0 41.0 36.0 41.0 26-27 39.33418733072183 41.0 39.0 41.0 36.0 41.0 28-29 39.26138474095069 41.0 39.0 41.0 36.0 41.0 30-31 39.14538411661121 41.0 39.0 41.0 36.0 41.0 32-33 38.99364658947685 40.0 39.0 41.0 35.0 41.0 34-35 38.91952037251339 40.0 38.0 41.0 35.0 41.0 36-37 38.7844956155279 40.0 38.0 41.0 35.0 41.0 38-39 38.65916943596979 40.0 38.0 41.0 35.0 41.0 40-41 38.59267143722535 40.0 38.0 41.0 35.0 41.0 42-43 38.48755652919509 40.0 38.0 41.0 35.0 41.0 44-45 38.41885271432962 40.0 38.0 41.0 34.5 41.0 46-47 38.389771077817585 40.0 38.0 41.0 34.5 41.0 48-49 38.480843504634755 41.0 39.0 41.0 35.0 41.0 50-51 38.43154337681019 41.0 39.0 41.0 35.0 41.0 52-53 38.41118908478572 41.0 39.0 41.0 34.5 41.0 54-55 38.350848874779366 41.0 39.0 41.0 34.0 41.0 56-57 38.282820625480895 40.0 38.5 41.0 34.0 41.0 58-59 38.144071147196286 40.0 38.0 41.0 34.0 41.0 60-61 37.94958630633752 40.0 38.0 41.0 33.5 41.0 62-63 37.780443941122314 40.0 38.0 41.0 33.0 41.0 64-65 37.53554196860837 40.0 37.0 41.0 33.0 41.0 66-67 37.29435887280596 39.0 37.0 41.0 32.5 41.0 68-69 36.99839480120495 39.0 36.0 41.0 32.0 41.0 70-71 36.647829217024665 39.0 36.0 40.5 32.0 41.0 72-73 36.276464464563574 37.5 35.5 40.0 31.5 41.0 74-75 35.8462460901428 37.0 35.0 39.0 31.0 41.0 76-77 35.16763136742034 36.0 35.0 39.0 30.5 40.0 78-79 35.042215112453306 36.0 35.0 38.5 31.0 40.0 80-81 34.69043179125609 36.0 35.0 37.0 31.0 39.0 82-83 34.345524799272795 35.5 35.0 37.0 30.5 39.0 84-85 33.962912309870404 35.0 34.0 36.5 30.0 38.0 86-87 33.68473159934733 35.0 34.0 36.0 30.0 37.0 88-89 33.41265504487612 35.0 34.0 36.0 29.5 37.0 90-91 33.246149276268156 35.0 34.0 36.0 29.0 36.5 92-93 33.10287368058435 35.0 34.0 35.0 29.0 36.0 94-95 32.95579704016332 35.0 34.0 35.0 29.0 36.0 96-97 32.855157367240196 35.0 34.0 35.0 29.0 36.0 98-99 32.78424361066246 35.0 34.0 35.0 29.0 36.0 100-101 32.38894939756054 35.0 33.5 35.0 27.5 35.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.148865981034767 1101 2 0.16305252963984884 1101 3 0.1484923832765901 1101 4 0.17852670328754527 1101 5 0.16702966352008275 1101 6 0.11759509284001979 1101 7 0.28317627038596527 1101 8 0.15736055752390854 1101 9 0.15937612216523434 1101 10-11 0.025679801235575894 1101 12-13 0.04978021405484867 1101 14-15 0.14075282931941757 1101 16-17 0.13962145132467185 1101 18-19 0.16344331200431128 1101 20-21 0.09802151700410633 1101 22-23 0.18677073753089246 1101 24-25 0.05716965779826921 1101 26-27 0.14961096116203976 1101 28-29 0.09909273914491479 1101 30-31 0.044152295540591524 1101 32-33 -0.02894465999768414 1101 34-35 -0.025371846679099974 1101 36-37 0.018719958896525668 1101 38-39 0.08874958344465256 1101 40-41 0.03339462061164511 1101 42-43 0.005392938724412488 1101 44-45 0.032125593903906235 1101 46-47 0.10692706511942873 1101 48-49 0.11171251064747167 1101 50-51 0.29212893211918356 1101 52-53 -0.049134750736520516 1101 54-55 0.10910519504578531 1101 56-57 0.2623211738783837 1101 58-59 0.11071623702557787 1101 60-61 -0.0755235451004026 1101 62-63 -0.05144124089083846 1101 64-65 0.028640610734136374 1101 66-67 0.08010898772396047 1101 68-69 0.0883055608393235 1101 70-71 0.16102788371846088 1101 72-73 0.054831402453572764 1101 74-75 0.08686473580153375 1101 76-77 0.10437542223650098 1101 78-79 0.2305063812923791 1101 80-81 0.25308019908150925 1101 82-83 0.25829406324497484 1101 84-85 0.10333416573879362 1101 86-87 -0.05392105117392987 1101 88-89 0.1296436390688811 1101 90-91 0.09956059174592014 1101 92-93 -0.07651704890378852 1101 94-95 0.0751694272197696 1101 96-97 0.08198966316520284 1101 98-99 -0.08799541637702646 1101 100-101 -0.01996711301212173 1102 1 0.09031600376871296 1102 2 0.09951547331147026 1102 3 0.10260866596023277 1102 4 0.06717786099204659 1102 5 -0.04761761502540196 1102 6 0.04787238939999838 1102 7 0.1643755866410217 1102 8 0.02931521625576039 1102 9 -0.014657972995031798 1102 10-11 0.035628779359193175 1102 12-13 -0.026628139374530235 1102 14-15 0.010660713681339473 1102 16-17 0.053441099207539366 1102 18-19 0.10826601601986852 1102 20-21 0.038036999107596614 1102 22-23 0.0160452945067604 1102 24-25 0.13473485241809158 1102 26-27 0.0423772504644262 1102 28-29 -0.23702214496952934 1102 30-31 -0.2692602996398179 1102 32-33 0.09757621206489375 1102 34-35 0.11662969002039603 1102 36-37 0.1314198881047446 1102 38-39 0.16577736489951178 1102 40-41 0.1577550485853294 1102 42-43 0.21616393596756467 1102 44-45 0.17025948244425848 1102 46-47 0.06209155527476895 1102 48-49 -0.02828973396975698 1102 50-51 0.10760916509894258 1102 52-53 -0.0726399219893068 1102 54-55 -0.14954809216737885 1102 56-57 -0.20810671347636145 1102 58-59 -0.09912525846504394 1102 60-61 0.19156432303120852 1102 62-63 0.005481388879694293 1102 64-65 0.14642330300862483 1102 66-67 0.22745012350029015 1102 68-69 0.061960085469685566 1102 70-71 0.16284947693626606 1102 72-73 0.20779055617531839 1102 74-75 0.2796651260196015 1102 76-77 0.23192651605574355 1102 78-79 -0.0178101557812127 1102 80-81 0.16125521016070365 1102 82-83 -0.12078725520866129 1102 84-85 -0.05894014950906268 1102 86-87 -0.10187312571824947 1102 88-89 0.10294492425613555 1102 90-91 0.14128673117694035 1102 92-93 0.1524662833743804 1102 94-95 0.17544338563104134 1102 96-97 0.4118757344519395 1102 98-99 0.17984713235706806 1102 100-101 0.03947567514780559 1103 1 0.15980155399699214 1103 2 0.1202027968557573 1103 3 0.14207271773102548 1103 4 0.09544076654197653 1103 5 0.027512473135992366 1103 6 0.11263359565840858 1103 7 0.1346403707498851 1103 8 0.008959162538239696 1103 9 0.11426188288214689 1103 10-11 0.06523210078226072 1103 12-13 0.0022100578087247413 1103 14-15 0.0765247894453438 1103 16-17 0.0650375880200258 1103 18-19 0.13961736516053946 1103 20-21 -0.0014822643009964054 1103 22-23 0.008244094573832683 1103 24-25 0.012014377352628003 1103 26-27 0.09234991729559283 1103 28-29 0.06259335027886692 1103 30-31 0.11982633628100814 1103 32-33 0.1584034187240988 1103 34-35 0.07223748835750854 1103 36-37 0.15860548509250805 1103 38-39 0.15564943748986337 1103 40-41 0.07167533388636116 1103 42-43 -0.027703482486209907 1103 44-45 0.11757088017117923 1103 46-47 0.13511337682258073 1103 48-49 0.004611630780132714 1103 50-51 0.1273507337365416 1103 52-53 0.23438449791360227 1103 54-55 0.11296927223352071 1103 56-57 0.23958264540064533 1103 58-59 0.3158103075087766 1103 60-61 0.41023137776979723 1103 62-63 0.22501478919406281 1103 64-65 0.22233317035074407 1103 66-67 0.208214320729887 1103 68-69 0.20437134809849056 1103 70-71 0.37704166251280213 1103 72-73 0.4000572461081262 1103 74-75 0.3081652371245056 1103 76-77 0.24872891701220112 1103 78-79 0.46340152836459225 1103 80-81 0.5295100180037622 1103 82-83 0.34543583252604293 1103 84-85 0.17627983137816017 1103 86-87 0.17808464078697028 1103 88-89 0.3443506534519116 1103 90-91 0.3076485977938006 1103 92-93 0.24660485231599694 1103 94-95 0.20613832974459712 1103 96-97 0.24499446946470727 1103 98-99 0.22566563466300238 1103 100-101 0.16310509736142365 1104 1 0.1365176500274501 1104 2 0.10514012473733914 1104 3 0.20463359321289687 1104 4 0.17152397878274428 1104 5 0.054208880431758644 1104 6 0.08146471687970092 1104 7 0.1514860900776469 1104 8 0.12830829026092516 1104 9 0.2031449848912814 1104 10-11 0.11545670405268282 1104 12-13 0.17471750508052253 1104 14-15 0.34496093505475045 1104 16-17 0.28826106685194475 1104 18-19 0.2754108527718131 1104 20-21 0.21255545590154412 1104 22-23 0.14237778708699267 1104 24-25 0.2015167106099014 1104 26-27 0.19310630602137735 1104 28-29 0.18506107244711956 1104 30-31 0.07815290128259988 1104 32-33 0.23642737767040245 1104 34-35 0.23927570220055117 1104 36-37 0.24430615438308223 1104 38-39 0.24537191797968205 1104 40-41 0.05867033996363347 1104 42-43 0.11953127739023728 1104 44-45 0.10067617602997103 1104 46-47 0.3206422516319307 1104 48-49 0.2621333278921796 1104 50-51 0.3454112132315075 1104 52-53 0.3899441478270447 1104 54-55 0.2760258108212241 1104 56-57 0.2549638768219751 1104 58-59 0.1467485468239218 1104 60-61 0.2541695545107956 1104 62-63 0.2491895847061727 1104 64-65 0.21563710436851835 1104 66-67 0.2581434349643672 1104 68-69 0.2758240733290833 1104 70-71 0.27415554051091107 1104 72-73 0.24034589623204283 1104 74-75 0.11714816702312447 1104 76-77 0.0640251992860783 1104 78-79 0.10273551187915331 1104 80-81 0.16178260363710706 1104 82-83 0.13790550776621302 1104 84-85 0.21815963465515154 1104 86-87 0.2857017476743593 1104 88-89 0.19070583569754262 1104 90-91 0.1432403943861047 1104 92-93 0.18573821931716594 1104 94-95 0.07109067542189251 1104 96-97 0.17336697906098664 1104 98-99 0.2896296880267428 1104 100-101 0.3115088561083752 1105 1 0.11290894398605644 1105 2 0.08364647562613214 1105 3 0.0293294409239806 1105 4 0.08919505673348738 1105 5 -0.00494891280667531 1105 6 -0.04982891670263001 1105 7 0.13734999814189308 1105 8 0.0887668374924857 1105 9 0.08325363886881121 1105 10-11 0.051938185868543485 1105 12-13 0.05575849348743134 1105 14-15 0.010804812073281767 1105 16-17 0.08954683367444716 1105 18-19 -0.08806414844702459 1105 20-21 -0.04593471606570887 1105 22-23 0.017766686107769658 1105 24-25 0.0790284911880974 1105 26-27 0.03865990140749176 1105 28-29 0.08439483553305394 1105 30-31 0.012516316696270735 1105 32-33 0.046103552462945174 1105 34-35 0.06376570202599652 1105 36-37 0.1007934625367568 1105 38-39 0.049025537381389483 1105 40-41 0.053597874887053365 1105 42-43 0.010190538348169298 1105 44-45 -0.11804472613750505 1105 46-47 -0.10717796973279548 1105 48-49 0.06819452490365308 1105 50-51 0.08055209564481203 1105 52-53 0.043002283001690955 1105 54-55 0.02566873696697769 1105 56-57 0.06413084895004317 1105 58-59 0.0282216954117942 1105 60-61 0.1719640208140376 1105 62-63 0.06195919578006226 1105 64-65 -0.014405431508912159 1105 66-67 -0.030150742128611796 1105 68-69 -0.027454264912698534 1105 70-71 -0.04177251143979532 1105 72-73 0.15127997013625105 1105 74-75 0.07297935008870837 1105 76-77 -0.11068006807240494 1105 78-79 -0.02996504986889903 1105 80-81 0.03511783895675791 1105 82-83 0.0073600289261079865 1105 84-85 -0.0011463088715046865 1105 86-87 0.012661079475272174 1105 88-89 0.008346277223196807 1105 90-91 -0.08058487326129438 1105 92-93 -0.24464486841315392 1105 94-95 -0.02818127310892038 1105 96-97 -0.02869438888849629 1105 98-99 -0.06935578284428345 1105 100-101 -0.08609536341214863 1106 1 0.0941891032079667 1106 2 0.15739073395890557 1106 3 0.06245743124230785 1106 4 0.1620653697322254 1106 5 0.09816298121379674 1106 6 -0.10614547608909675 1106 7 0.12772977176956601 1106 8 0.1759445914871165 1106 9 0.1889293334590576 1106 10-11 0.2230035969215649 1106 12-13 0.1352107072831359 1106 14-15 0.07455844142145907 1106 16-17 0.034617150428417176 1106 18-19 0.033378407569962576 1106 20-21 0.08684864619999644 1106 22-23 0.1290974215020455 1106 24-25 0.10885955687226101 1106 26-27 0.10022696049609436 1106 28-29 0.10440169736946103 1106 30-31 0.09739228302888847 1106 32-33 0.11532360746004855 1106 34-35 -0.004075157990833134 1106 36-37 0.16022259871348865 1106 38-39 0.20324360381147244 1106 40-41 0.2585005033800627 1106 42-43 0.23355611548494437 1106 44-45 0.15353037256539892 1106 46-47 0.06426211124370695 1106 48-49 0.17940748250877192 1106 50-51 0.199629707794017 1106 52-53 0.17556374508492212 1106 54-55 0.20895914114962721 1106 56-57 0.029168768277386903 1106 58-59 0.127697840288711 1106 60-61 0.08513447941344054 1106 62-63 -0.0016126461109351453 1106 64-65 0.07417114709752326 1106 66-67 0.16780470372859213 1106 68-69 0.013631407333903667 1106 70-71 0.055312232239884906 1106 72-73 0.0967556814543471 1106 74-75 0.21774788259453004 1106 76-77 0.1678789121426334 1106 78-79 0.15458421111815568 1106 80-81 0.13462897578553168 1106 82-83 0.1304105078037665 1106 84-85 0.011803273700117245 1106 86-87 0.05165491577096759 1106 88-89 0.14179619349854988 1106 90-91 0.25050470784237433 1106 92-93 0.21508024846850304 1106 94-95 0.266300752122838 1106 96-97 0.20406354020245487 1106 98-99 0.29732232021640925 1106 100-101 0.3542988337273911 1107 1 0.031309389443514135 1107 2 0.1641021973896457 1107 3 0.14186782724762992 1107 4 0.13032439872774404 1107 5 0.17988406580432326 1107 6 0.03694975165462466 1107 7 0.12506805174773206 1107 8 0.10929209489678726 1107 9 0.044016149330190046 1107 10-11 0.01400302206733528 1107 12-13 0.12091503253791558 1107 14-15 0.13033701465580094 1107 16-17 0.18692775850182386 1107 18-19 0.09672186928741411 1107 20-21 0.13069939665046348 1107 22-23 0.16597101959037275 1107 24-25 0.22678921210463443 1107 26-27 0.2116126168363195 1107 28-29 0.0740584111677336 1107 30-31 0.17526007299615998 1107 32-33 0.2976981257982203 1107 34-35 0.2806419316043076 1107 36-37 0.2737377920947779 1107 38-39 0.28541233399550947 1107 40-41 0.28885425611544946 1107 42-43 0.28774860631062893 1107 44-45 0.22454660857446385 1107 46-47 0.3441219241020832 1107 48-49 0.2671878849711433 1107 50-51 0.1445362899555036 1107 52-53 0.15924722422512616 1107 54-55 0.19273037814898686 1107 56-57 0.2598503650517614 1107 58-59 0.23343317868784652 1107 60-61 0.3573311793958922 1107 62-63 0.26088882322936513 1107 64-65 0.040260383153288615 1107 66-67 0.17628340111212282 1107 68-69 0.26776528557108037 1107 70-71 0.24041288003780892 1107 72-73 0.20961711904080005 1107 74-75 0.20622664438749183 1107 76-77 0.08318533199233968 1107 78-79 0.25887033716420405 1107 80-81 0.28241884643527726 1107 82-83 0.18341383938194866 1107 84-85 0.32639909642602305 1107 86-87 0.16342058779488866 1107 88-89 0.10418671758204567 1107 90-91 0.1360724850887749 1107 92-93 0.27928840688995393 1107 94-95 0.3894451564724122 1107 96-97 0.18372223459093817 1107 98-99 0.26466351472164007 1107 100-101 0.3739702811845902 1108 1 0.11410265677388765 1108 2 0.04949329710191819 1108 3 0.029318072230765324 1108 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-1.2159715927055075 2316 76-77 -1.0825152669696365 2316 78-79 -1.182909092922479 2316 80-81 -1.3766292104361284 2316 82-83 -1.7275047244418147 2316 84-85 -1.5973615053342272 2316 86-87 -1.1976555450027746 2316 88-89 -1.018847001512114 2316 90-91 -0.8363046410930011 2316 92-93 -0.9294754928255529 2316 94-95 -0.9722106169480682 2316 96-97 -0.785030472064264 2316 98-99 -0.9149817718673425 2316 100-101 -1.025135606498381 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 6.0 13 25.0 14 99.0 15 348.0 16 991.0 17 2069.0 18 3412.0 19 4315.0 20 5812.0 21 7580.0 22 8234.0 23 6409.0 24 5308.0 25 5233.0 26 6174.0 27 7733.0 28 9726.0 29 11816.0 30 14507.0 31 18357.0 32 23389.0 33 30395.0 34 42406.0 35 64124.0 36 108959.0 37 254152.0 38 586336.0 39 226392.0 40 30.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 35.87935446590721 27.13430741291068 15.422307522976169 21.56403059820593 2 12.671017409618713 24.324164501265546 43.65613682355425 19.348681265561492 3 13.501878183164632 33.43757370227273 33.76225907307209 19.298289041490555 4 9.03279313081355 21.24408415661151 47.26004128883895 22.463081423735993 5 8.392827797133677 43.19191494131003 39.3791810288812 9.036076232675097 6 22.28788788293222 47.4320601071141 20.356561099662596 9.92349091029108 7 22.016011419636577 35.217697136220835 29.608749553920443 13.157541890222143 8 21.242050501362478 39.8581621728664 24.066980349121284 14.83280697664984 9 24.869476606866222 16.934726958057176 26.258288140919195 31.937508294157407 10-11 19.77860014563337 29.67200861973532 34.448515027809925 16.10087620682139 12-13 20.62843756295824 29.15342179976168 32.22526828376092 17.99287235351916 14-15 20.263185217731518 30.774710400684295 28.89956041825244 20.062543963331745 16-17 17.766927472793444 33.54040363409581 30.43816529456378 18.254503598546968 18-19 17.322498155516914 32.825679330169 32.85957793826328 16.992244576050805 20-21 17.9956571276121 32.49625086895266 32.53028699675522 16.97780500668002 22-23 18.97378102804114 31.31719898687315 31.639276233841557 18.069743751244147 24-25 18.808089184281222 32.14371909674305 31.152992738271802 17.89519898070392 26-27 18.161058956761742 31.849461300922687 32.247787135993924 17.741692606321642 28-29 18.233336474031812 29.879665398695625 33.79691876847023 18.090079358802335 30-31 18.9824576605721 31.659596914066746 31.63965305945607 17.718292365905086 32-33 17.865919567417706 32.10650771211054 32.104823094525614 17.922749625946135 34-35 17.959351924622695 31.75209734745111 32.442446283082944 17.846104444843252 36-37 18.954444533832255 30.45219230480968 31.39286148946221 19.200501671895854 38-39 18.0926085219588 32.43233858452339 32.14994186354332 17.325111029974483 40-41 17.139906362830622 31.191463876666823 34.19853857805997 17.470091182442584 42-43 17.99345681227941 31.105685958618945 31.608767431482526 19.292089797619123 44-45 18.349598476831712 31.320526122899988 31.87008925716667 18.459786143101635 46-47 18.409557069647544 30.78110506711993 32.15282977741747 18.656508085815048 48-49 17.566744473742947 31.28855603158461 32.00797628575133 19.136723208921115 50-51 17.967478584560943 30.512406049034162 30.938153195390157 20.58196217101474 52-53 17.88044999198948 30.744352925078577 32.24355840496391 19.131638677968034 54-55 18.805719635280596 30.864487852943313 31.338694588927428 18.991097922848667 56-57 18.82046595802761 31.276829235589826 30.504862353085976 19.397842453296587 58-59 17.859684516037206 30.62257922338495 31.23426688587308 20.28346937470476 60-61 19.411222918492385 32.34547162915873 29.895385283942193 18.34792016840669 62-63 18.1275041479382 31.8588470210137 31.94565633687378 18.06799249417432 64-65 18.098556249342483 30.90384140677161 31.293090941095496 19.704511402790413 66-67 18.462811576684082 31.22182135227255 30.55481638712348 19.760550683919888 68-69 18.127813567281862 31.612308563971077 30.818269768286164 19.441608100460897 70-71 18.417395693020254 30.3650048097518 32.29605655360484 18.921542943623106 72-73 18.75342510023468 31.00546847120028 30.4210097109542 19.820096717610845 74-75 17.912973403000816 31.275178999090308 30.30198640342644 20.509861194482433 76-77 17.466481290099885 31.62509789684234 31.893708267066025 19.014712545991745 78-79 17.713244296106367 31.529966778642205 30.753598464728395 20.003190460523037 80-81 18.06500726184451 32.0382470897608 29.863785401491977 20.032960246902714 82-83 18.686385259327412 31.375879999614924 30.432893110044816 19.50484163101284 84-85 17.844041649218852 30.839310283655024 30.674149113994897 20.642498953131224 86-87 17.175077028226607 32.048933637801966 31.510234560490453 19.265754773480975 88-89 18.61140162149488 30.364110931647893 30.899578295814518 20.124909151042708 90-91 17.981011278678878 30.853852992807028 31.862731952772982 19.302403775741112 92-93 18.703574205978395 31.960680365004812 30.317560510390646 19.01818491862615 94-95 18.186603242577203 30.13404045967338 31.010041001501026 20.669315296248396 96-97 18.885272120560778 30.079307615772688 30.707050704204047 20.328369559462487 98-99 17.961414720247095 30.86798314283416 30.656649731114587 20.513952405804154 100-101 17.87842157629215 30.67624628954637 31.01076297996957 20.43456915419191 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 456.0 1 291.0 2 232.0 3 467.5 4 389.5 5 232.5 6 433.0 7 821.5 8 1162.0 9 1202.0 10 989.5 11 754.5 12 707.0 13 733.5 14 745.0 15 763.0 16 797.0 17 845.5 18 869.0 19 929.5 20 1111.5 21 1324.0 22 1569.0 23 1933.5 24 2241.0 25 2833.5 26 4396.5 27 6864.5 28 8663.5 29 13822.0 30 23756.0 31 33169.0 32 54533.0 33 79597.5 34 96043.0 35 109567.5 36 132852.0 37 147851.5 38 132876.5 39 114547.0 40 92803.0 41 70913.0 42 56261.0 43 48203.5 44 44660.5 45 39651.5 46 31389.5 47 21855.0 48 18718.5 49 15230.5 50 11085.5 51 7088.0 52 2906.5 53 1528.0 54 1232.5 55 1218.0 56 1026.5 57 877.0 58 924.5 59 808.5 60 616.0 61 602.0 62 554.5 63 375.5 64 208.5 65 128.5 66 95.0 67 85.5 68 52.5 69 20.5 70 14.5 71 11.5 72 8.0 73 6.5 74 6.0 75 8.0 76 7.0 77 2.5 78 1.0 79 2.0 80 2.5 81 2.5 82 1.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04517522417431448 2 0.0017877562078115318 3 4.1255912487958427E-4 4 0.013133132142000101 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001890895989031428 24-25 0.0 26-27 0.0 28-29 0.021178035077151994 30-31 0.017533762807382332 32-33 6.875985414659739E-5 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.03627082306233012 50-51 0.01289247265248701 52-53 0.0 54-55 9.282580309790647E-4 56-57 0.0 58-59 0.0 60-61 3.4379927073298694E-5 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 1.0313978121989607E-4 80-81 0.011070336517602178 82-83 0.005156989060994804 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1454337.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.887842611635639 #Duplication Level Percentage of deduplicated Percentage of total 1 72.45558024579083 7.1642937380511595 2 9.673039282324284 1.9129097999958296 3 3.725210159015728 1.1050287524284104 4 1.9971435585190978 0.7898976471791507 5 1.2770373978113805 0.631357239936583 6 0.9566466360340704 0.5675502823233343 7 0.6774927828421496 0.46892594050835523 8 0.561463346056079 0.44413289584038596 9 0.46847024076954763 0.4168944008067906 >10 5.063086516951215 11.252604469362582 >50 1.2556643681039628 8.813614734729965 >100 1.5582426328784502 32.49565438076885 >500 0.23029961604615568 15.624444141827773 >1k 0.09849737424743274 16.98121489064771 >5k 0.0021258426096568213 1.3314766855931244 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC 7148 0.4914954374398781 No Hit ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC 6386 0.4391004285801709 No Hit GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT 5469 0.37604764232774107 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 4781 0.3287408626748821 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4082 0.28067772462641055 No Hit TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA 3956 0.27201398300393925 No Hit GATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTAATGCTACT 3932 0.2703637465044209 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3685 0.25338006253021134 No Hit TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC 3563 0.2449913603243265 No Hit CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT 3435 0.23619009899356203 No Hit GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA 3288 0.22608240043401218 No Hit GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC 3226 0.22181928947692317 No Hit CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA 3208 0.22058161210228444 No Hit ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT 3108 0.2137056266876247 No Hit ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG 3028 0.20820483835589687 No Hit ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC 2935 0.20181017192026332 No Hit CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG 2585 0.17774422296895423 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 2538 0.17451250982406416 No Hit GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT 2519 0.17320607259527881 No Hit GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC 2500 0.17189963536649344 No Hit CATCAATAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAA 2451 0.1685304025133102 No Hit CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC 2422 0.16653636674305886 No Hit TAATTACCCTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTC 2413 0.1659175280557395 No Hit GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC 2343 0.16110433826547768 No Hit CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT 2279 0.15670370760009544 No Hit TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC 2260 0.1553972703713101 No Hit CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT 2245 0.15436587255911113 No Hit ATCTAGGAGGCTGCTGACCTCCAACAGGAATTTCACCACTTAACCCTCTA 2192 0.15072160028934148 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2182 0.15003400174787548 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 2177 0.1496902024771425 No Hit CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA 2152 0.14797120612347758 No Hit CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA 2137 0.14693980831127862 No Hit ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT 2127 0.14625220976981265 No Hit CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT 2106 0.1448082528327341 No Hit CTATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGC 2084 0.14329553604150896 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 2066 0.1420578586668702 No Hit GACATATAGGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATT 2056 0.1413702601254042 No Hit GGATATGAGATTGGCTTGAAACCAATTTTAGGGGGTTCGATTCCTTCCTT 2052 0.14109522070881783 No Hit GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT 2039 0.14020134260491207 No Hit CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT 2004 0.13779474770978115 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 2002 0.13765722800148797 No Hit ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA 2000 0.13751970829319476 No Hit CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC 1992 0.136969629460022 No Hit ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC 1982 0.13628203091855604 No Hit ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA 1954 0.13435675500245128 No Hit ACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAAAAC 1895 0.13029992360780204 No Hit GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA 1885 0.12961232506633608 No Hit CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG 1878 0.12913100608730987 No Hit ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT 1844 0.1267931710463256 No Hit ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG 1834 0.1261055725048596 No Hit ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA 1800 0.12376773746387529 No Hit ACTACATAGTATGTATCGTGAAGCACGATGTCAAGGGATGAGTTGGATAA 1797 0.1235614579014355 No Hit CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC 1791 0.12314889877655592 No Hit GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA 1766 0.12142990242289098 No Hit GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG 1765 0.12136114256874439 No Hit ATATAAGCCTCGTCCGACATGAAGGAATAAGCAAATAAAAAATATTGAGG 1746 0.12005470533995903 No Hit CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA 1721 0.1183357089862941 No Hit GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA 1716 0.1179919097155611 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 1714 0.11785439000726791 No Hit TTCCAATACTTATTATTATTGAAACAATTAGCCTATTTATTCAACCAATG 1712 0.11771687029897474 No Hit GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG 1702 0.11702927175750875 No Hit ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT 1689 0.116135393653603 No Hit CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG 1683 0.11572283452872341 No Hit CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG 1678 0.11537903525799041 No Hit CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT 1664 0.11441639729993804 No Hit CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT 1650 0.11345375934188569 No Hit CTTATTAATACACCTAATCGGAGGAGCTACTCTAGTATTAATAAATATTA 1635 0.11242236152968672 No Hit GAATTATACCATATCGTAGTCCTTTTTGTACAATAGGAGTGTGGTGGCCT 1615 0.11104716444675478 No Hit GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA 1609 0.11063460532187519 No Hit GATTAGCATTATTTGTTTGATAATAAGTTTAACTAGTCAGTGTTGGAAAG 1566 0.1076779315935715 No Hit TCTCCATTCTTTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAA 1563 0.10747165203113171 No Hit ATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCACCTTC 1556 0.10699033305210552 No Hit GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT 1549 0.10650901407307935 No Hit ATATAGGAATAGTATGAGCAATAATGTCTATTGGCTTTCTAGGCTTTATT 1543 0.10609645494819976 No Hit ACCCTACACGGAGGTAATATTAAATGATCTCCAGCTATACTATGAGCCTT 1513 0.10403365932380183 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 1510 0.10382737976136205 No Hit CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT 1505 0.10348358049062907 No Hit CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT 1492 0.10258970238672331 No Hit ATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTGAATTA 1486 0.10217714326184371 No Hit GTATAGTAGGGGTGAAATGGAATTTTATCTGCATCTGAGTTTAATCCTGT 1476 0.10148954472037773 No Hit TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC 1469 0.10100822574135157 No Hit GTATTATCATGTAGATATAGGCTTACTAGGAGGGTGAATACGTAGGCTTG 1460 0.10038938705403218 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.875985414659739E-5 0.0 8 0.0 0.0 0.0 6.875985414659739E-5 0.0 9 0.0 0.0 0.0 6.875985414659739E-5 0.0 10-11 0.0 0.0 0.0 3.437992707329869E-4 0.0 12-13 0.0 0.0 0.0 0.0013408171558586491 0.0 14-15 0.0 0.0 0.0 0.0024065948951309084 0.0 16-17 0.0 0.0 0.0 0.007185404758319427 0.0 18-19 0.0 0.0 0.0 0.011586035423701659 0.0 20-21 0.0 0.0 0.0 0.021487454420811684 0.0 22-23 0.0 0.0 0.0 0.04575968293456056 0.0 24-25 0.0 0.0 0.0 0.07144148845831469 0.0 26-27 0.0 0.0 0.0 0.08289000417372315 0.0 28-29 0.0 0.0 0.0 0.09241324397302689 0.0 30-31 0.0 0.0 0.0 0.1105314655406553 0.0 32-33 0.0 0.0 0.0 0.14092332107345135 0.0 34-35 0.0 0.0 0.0 0.1868892835704517 0.0 36-37 0.0 0.0 0.0 0.24918571142726892 0.0 38-39 0.0 0.0 0.0 0.34342109153518063 0.0 40-41 0.0 0.0 0.0 0.4551902344504747 0.0 42-43 0.0 0.0 0.0 0.5524510481408367 0.0 44-45 0.0 0.0 0.0 0.6680363629612669 0.0 46-47 0.0 0.0 0.0 0.8100598417010638 0.0 48-49 0.0 0.0 0.0 0.9595781445428397 0.0 50-51 0.0 0.0 0.0 1.1180008484966 0.0 52-53 0.0 0.0 0.0 1.2740169575552296 0.0 54-55 0.0 0.0 0.0 1.427592091791655 0.0 56-57 0.0 0.0 0.0 1.6001105658454677 0.0 58-59 0.0 0.0 0.0 1.8036053541923227 0.0 60-61 0.0 0.0 0.0 2.0294470951368218 0.0 62-63 0.0 0.0 0.0 2.2409867864188286 0.0 64-65 0.0 0.0 0.0 2.4609151799067206 0.0 66-67 0.0 0.0 0.0 2.707281737313979 0.0 68-69 0.0 0.0 0.0 2.9803614980571904 0.0 70-71 0.0 0.0 0.0 3.313606131178674 0.0 72-73 0.0 0.0 0.0 3.6365711661052424 0.0 74-75 0.0 0.0 0.0 3.9617365163645015 0.0 76-77 0.0 0.0 0.0 4.322656990779992 0.0 78-79 0.0 0.0 0.0 4.747214710208157 0.0 80-81 0.0 0.0 0.0 5.228739968796779 0.0 82-83 0.0 0.0 0.0 5.71339380074907 0.0 84-85 0.0 0.0 0.0 6.157960637733895 0.0 86-87 0.0 0.0 0.0 6.634157007626156 0.0 88-89 0.0 0.0 0.0 7.1585196553481065 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTAAGG 15 6.616187E-4 95.00757 3 GTTATAT 115 0.0 61.987034 1 ATGTATT 120 0.0 59.404243 1 GACATAT 435 0.0 55.71708 1 GGGTCAC 45 1.516999E-5 52.772907 8 TACCTGG 155 0.0 52.10092 2 ACATATA 485 0.0 49.952435 2 CTCAATT 130 0.0 47.49562 9 AGCGTGT 35 7.932431E-8 47.495617 20-21 GGATAGC 70 7.928793E-8 47.495617 8 GGAACTT 50 2.8289109E-5 47.495617 8 TGGGTAT 320 0.0 46.011383 6 GATGGCC 170 0.0 44.701756 9 ACCTGGG 185 0.0 43.652122 3 TAACTTT 1435 0.0 41.70347 6 TAGCGTG 40 2.2658423E-7 41.558666 18-19 CTTAGCG 40 2.2658423E-7 41.558666 16-17 GGATGAC 745 0.0 41.43913 8 GGGGTCA 60 8.296348E-5 39.57968 7 CATGGGG 935 0.0 39.120766 4 >>END_MODULE