FastQCFastQC Report
Fri 10 Feb 2017
SRR2031585_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031585_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584176
Sequences flagged as poor quality0
Sequence length101
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC184410.7136123855341122No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC132310.5120007306003925No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA120050.46455814155073033No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC115690.44768622570598904No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC112710.4361545034084366No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA111770.4325169802675979No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT104210.40326200692212916No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC100160.38758969977277086No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT99800.38619660580393905No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG98780.3822495062255822No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT88820.3437072397545678No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA81190.3141813870262707No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA75750.29313018927503387No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT73370.28392029025886784No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA72490.2805149494461678No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT71440.27645175870374156No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC71420.276374364594362No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT71040.27490387651615056No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT70990.27471039124270175No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC70920.2744395118598733No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA69600.26933150064082323No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT67420.2608955427184526No Hit
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC64370.2490929410380717No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC62560.24208877413922272No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC59830.23152447820891459No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA59640.2307892341698089No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA59060.22854480499780203No Hit
GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT58740.22730649924772925No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA56960.22041842351294957No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG56710.21945099714570523No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA55080.2131433772312722No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT54890.2124081331921665No Hit
TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT53660.20764839546532432No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC53380.2065648779340107No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG53340.2064100897152516No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC53270.2061392103324232No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT50280.19456879098018093No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA49900.19309830290196953No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT49040.18977035619864902No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT48340.18706156237036486No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA47600.18419798032332163No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT47360.18326925101076708No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC47300.18303706868262842No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA47240.18280488635448977No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC46150.1785869073933045No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA44470.17208580220542255No Hit
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC44430.17193101398666344No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT43520.16840958200989406No Hit
GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG43420.16802261146299632No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT41850.1619471738767019No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC41600.16097974750945754No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA40950.15846443895462228No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT40470.15660698032951315No Hit
GTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTT38840.15029936041508007No Hit
AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG37990.14701011076644932No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT37940.14681662549300048No Hit
AGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTA37860.14650704905548229No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG37690.14584919912575614No Hit
GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC37240.14410783166471633No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG37130.14368216406312884No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC37060.14341128468030043No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG36760.1422503730396072No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA36560.14147643194581175No Hit
TGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCTATTGTGCTTG35790.1384967587346992No Hit
AGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAAT35720.13822587935187078No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT35630.13787760585966283No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC35410.1370262706564878No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA35080.13574926785172525No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG35020.13551708552358663No Hit
CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT34180.13226653292964566No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA34030.13168607710929905No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA33890.13114431834364224No Hit
ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC33850.13098953012488312No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT32840.127081127601216No Hit
GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA32750.12673285410900806No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC31960.12367578678851596No Hit
CATCTATTGTGCTTGTATGTGTTAGTTTTGTTGTTAATATTAGCGAGATG31690.12263096631189208No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC31040.12011565775705679No Hit
ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA31040.12011565775705679No Hit
ACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAG30920.11965129310077952No Hit
GTTGGGTAGTAGGTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTT30470.1179099256397397No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA30090.11643943756152833No Hit
GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT29570.11442719071766011No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC29530.11427240249890101No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG29500.11415631133483167No Hit
GGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTA29320.11345976435041576No Hit
CCTTTAGGCATTCCGGTGTTGGGTTAACAGAGAAGTTATAGGTGGATTAT29240.11315018791289758No Hit
GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT29240.11315018791289758No Hit
ATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGC29190.11295670263944871No Hit
ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA29150.11280191442068961No Hit
TCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTC28530.11040269702992365No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC28180.1090483001157816No Hit
ATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGT27860.10780999436570883No Hit
GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT27780.10750041792819065No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC27750.10738432676412134No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC27710.10722953854536224No Hit
CCATTATACTAGGACTTTACTTCACCACCCTCCAAGCTTCAGAATACTTT27640.1069586591625338No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG27530.10653299156094631No Hit
GGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGG27290.10560426224839176No Hit
ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG27190.10521729170149402No Hit
ATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTT26920.10417247122487014No Hit
CAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTACCAGCTGTA26910.10413377417018035No Hit
ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT26690.10328243896700535No Hit
GTGTTGGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTAT26520.10262458903727921No Hit
GTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAA26310.10181195088879394No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA26170.10127019212313713No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACTTT35950.054.319326
AACTTTA38200.051.2412837
TACCTGG2600.051.239252
ATTGAAC6000.049.0966458
ACCTTAA1001.0913936E-1147.6347921
ACCTGGG2800.047.5267033
AACTTGC4400.046.4303447
GTTATAT2450.044.7183721
TGGGTAT2500.043.7118536
TATCACG459.548593E-442.2927132
ATGTATT1253.6379788E-1241.9186171
ACTTTAT47850.041.009038
CATGGGT13150.039.3955234
TACTTGT4250.036.890217
TACTCGC1950.036.5462467
GATCCCC2350.036.392859
ATAACTT56950.036.302935
GGATGAC5650.036.1602488
ATACTCG2000.035.6346636
ATCAATA10850.035.520032
Produced by FastQC (version 0.11.5)