FastQCFastQC Report
Fri 10 Feb 2017
SRR2031585_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031585_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2584176
Sequences flagged as poor quality0
Sequence length101
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC195830.7578044219898334No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT185170.716553361690535No Hit
ACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTC138630.5364572691643293No Hit
GAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGAAGA129730.502016890490431No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA127310.49265220325550585No Hit
ATTATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTAC104310.40364897746902684No Hit
GCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACTAATTACC103300.3997405749453598No Hit
ATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTAT98730.38205602095213326No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG98170.379888985889506No Hit
GTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAAC96910.3750131569985945No Hit
TTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTT96560.3736587600844525No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA84850.3283445090427278No Hit
CTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATCATCTCGCT79480.3075641906743194No Hit
CAATAAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTA77400.2995152032988465No Hit
GGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCTTTCCCT76300.2952585272829714No Hit
GTGTAAATGTATGTGGTAAAAGGCCTAGGAGATTTGTTGATCCAATAAAT74710.28910569558729743No Hit
CATCATAGCCTTATAGAAGGTAAACGAAACCACATAAATCAAGCCCTACT74160.28697735757935994No Hit
GAATAGGATTGAAGGAAATATAATGATGGCTACAACGATTGGGAATCCTA72140.2791605525320257No Hit
CCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCA72050.2788122790398177No Hit
CCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTGTTATACGCGTAT69120.2674740420157141No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC68590.26542309811715614No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT64410.2492477292568308RNA PCR Primer, Index 33 (95% over 22bp)
CATTTATCCTACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGC64080.2479707264520683No Hit
ATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATC63920.2473515735770319No Hit
TTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCC63840.24704199713951372No Hit
GTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTACCTTCT62300.24108265071728863No Hit
CTATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAA61160.23667118648265442No Hit
TTCCAACACTGACTAGTTAAACTTATTATCAAACAAATAATGCTAATCCA61030.2361681247716874No Hit
ATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATAC60870.23554897189665103No Hit
TTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTTC60050.23237581341208957No Hit
ACTAAAAGTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTA59890.2317566605370532No Hit
ATATATAATAGACGAAATCAACAACCCCGTATTAACCGTTAAAACCATAG59240.22924135198221793No Hit
TTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGT59130.22881568438063044No Hit
ACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTC56220.21755484146590634No Hit
GTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGTCTTTCATCT54580.2112085244967835No Hit
GGATTACAGCTGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCT54370.21039588634829826No Hit
ATATCATCTCGCTAATATTAACAACAAAACTAACACATACAAGCACAATA53490.2069905455355982No Hit
CTCTAGAAGTCCCACTACTTAATACTTCAGTACTTCTAGCATCAGGTGTT51800.2004507432930265No Hit
CCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTT51100.19774194946474236No Hit
ATCACACACTAATAATTGTTTTCCTAATTAGCTCCTTAGTCCTCTATATC51000.1973549789178446No Hit
GTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAATAAGG49130.19011862969085697No Hit
CCTTAGTCCTCTATATCATCTCGCTAATATTAACAACAAAACTAACACAT47840.18512670963587619No Hit
CTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCCAATTCTCC47330.18315315984669775No Hit
AGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAA46890.18145048944034772No Hit
GTATCATGCTGCGGCTTCAAATCCGAAGTGATGTTTTGATGTGAAGTGAA45110.17456241370556805No Hit
CAATAATAGGATTCCCAATCGTTGTAGCCATCATTATATTTCCTTCAATC44790.1733241079554953No Hit
ATTACATGGAGTCCATGGAATCCAGTAGCCATGAAGAATGTAGAACCATA44510.17224059042418163No Hit
ACATAGACGAGTTGATTCATAAAATTGTTTTTAGGTAGCTCGTTTGGTTT43540.1684869761192736No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA43500.16833218790051452No Hit
CATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGCT41800.16175368860325304No Hit
GTCCTAGTATAATGGTAATTAGTAGGGCTTGATTTATGTGGTTTCGTTTA41680.16128932394697573No Hit
GTATTGGAGAAAGAAATTCGTACATCTAGGAGCTATAGAACTAGTACCGC41030.15877401539214048No Hit
CTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCT40280.15587173629040746No Hit
CTACTTAATACTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGC39460.15269857780584603No Hit
GTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTT39450.15265988075115627No Hit
GTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGTTATCCGAGTTG39190.15165375732922215No Hit
AGTTAGTTCATTATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTA38970.15080242212604714No Hit
ATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTATAAAG37990.14701011076644932No Hit
AACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAGAACAAG37410.1447656815944425No Hit
GTATCTATGGTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCAT37390.14468828748506296No Hit
CCCTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTG37080.14348867878967997No Hit
GGGTTAACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCT36850.14259864653181517No Hit
TACTAGGACTTTACTTCACCATCCTCCAAGCTTCAGAATACTTTGAAACA36580.1415538260551913No Hit
GATGAAAGACTAATTAAAAGTAAGAACAAGCAAAGATTAAACCTTGTACC35950.13911591160973555No Hit
AGTTAGAAGTTTTCTAGTTAGTTCATTATGCAAAAGGTACAAGGTTTAAT35750.13834197051594008No Hit
CTATAAGGCTATGATGAGCTCATGTAATTGAAACACCTGATGCTAGAAGT34890.13501402381261957No Hit
CCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAGC34830.13478184148448094No Hit
CCATAAAAGTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTG34740.134433567992273No Hit
TGGTAGAATAGTTCAAATGGTTTCAACTTCTTGTGCATCTATTGTGCTTG33920.13126040950771153No Hit
CTATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTT33340.1290159803357047No Hit
GTATAGGAAAAGTCAGACTACGTCTACAAAATGTCAGTATCATGCTGCGG33120.12816464513252968No Hit
CTATTAACCCTTGGCCTACTCACCAATATCCTCACAATATATCAATGATG32630.12626848945273078No Hit
TGCTTACACCACATGAAACACTGTCTCTTCTATAGGATCATTTATTTCAC32060.12406275733541369No Hit
ACCTAAAAATCTTCTCACTATTTTGCCACATAGACGAGTTGATTCATAAA31660.12251487514782275No Hit
CAATAGATGCACAAGAAGTTGAAACCATTTGAACTATTCTACCAGCTGTA31500.12189572227278639No Hit
GTGTTGGAAAGAATGGAGACGGTTGTTGATTAGGCGTTTTGAGGATGGGA30940.11972868721015907No Hit
GTATAATTCTATTCATCGTCTCGGAAGTATTTTTCTTTGCAGGATTCTTC30910.11961259604608973No Hit
CATCTATTGTGCTTGTATGTGTTAGTTTTGTTGTTAATATTAGCGAGATG30610.11845168440539654No Hit
GTATATTCGTAGCTTCAGTATCATTGGTGCCCTATGGTTTTAACGGTTAA30470.1179099256397397No Hit
CTATTATTGTTGGGGTAATGAATGAGGCAAATAGATTTTCGTTCATTTTA30320.11732946981939311No Hit
ATTTATCTTATTTCAACTAAAAGTCTCATCACAAACATTCCCACTGGCAC30030.11620725523338968No Hit
TCAGAATACTTTGAAACATCATTCTCCATTTCAGATGGTATCTATGGTTC29770.11520113181145557No Hit
CTTCAGTACTTCTAGCATCAGGTGTTTCAATTACATGAGCTCATCATAGC29680.1148528583192476No Hit
GTAAAAACCCCTTGAGAATTAAAATGAACGAAAATCTATTTGCCTCATTC29650.11473676715517828No Hit
ATCCTATTCCCATCCTCAAAACGCCTAATCAACAACCGTCTCCATTCTTT29620.11462067599110896No Hit
CCATTATACTAGGACTTTACTTCACCACCCTCCAAGCTTCAGAATACTTT29480.11407891722545213No Hit
TCATTATATTTCCTTCAATCCTATTCCCATCCTCAAAACGCCTAATCAAC29200.11299539969413849No Hit
GTTTTAGGTCGTTTGTTGGGATTATATATGAATCAAAGCATAGGTCTTCA28870.11171839688937596No Hit
CTTCTATAGGATCATTTATTTCACTAACAGCTGTTCTCATCATGATCTTT28830.11156360867061686No Hit
GTTCTACATTCTTCATGGCTACTGGATTCCATGGACTCCATGTAATTATT28680.11098315285027027No Hit
GAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGTTTCAA28260.10935787655329976No Hit
ACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAACTAAAAG28230.10924178538923046No Hit
ATACGATACTGATATTACTTCTCGTTTTGAAGCAAAGGCCTCTCAAATTA28180.1090483001157816No Hit
ACCATAGATACCATCTGAAATGGAGAATGATGTTTCAAAGTATTCTGAAG28130.10885481484233271No Hit
GCCATGAAGAATGTAGAACCATAGATACCATCTGAAATGGAGAATGATGT27630.10691996210784405No Hit
ATATATAGAATGCGTAGAGAGGGGAGAGCAATTATGATAAGGATTACAGC27580.10672647683439519No Hit
ACAGAGAAGTTATAGGTGGATTATTTATAGTGTGATTATTGCCTATAGTC27360.10587514163122017No Hit
CTCCTAGATCATGTGTTGGTACGAGGCTAGAATGATAGAACGCTCAGAAG27080.10479162409990651No Hit
ATGCAAAAGGTACAAGGTTTAATCTTTGCTTGTTCTTACTTTTAATTAGT27010.10452074471707809No Hit
TCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTC26670.1032050448576258No Hit
ACATAAATCAAGCCCTACTAATTACCATTATACTAGGACTTTACTTCACC26410.10219892143569168No Hit
AAGGAATGTTGATCCAATAATTACATGGAGTCCATGGAATCCAGTAGCCA26170.10127019212313713No Hit
CGCTAATATTAACAACAAAACTAACACATACAAGCACAATAGATGCACAA26140.1011541009590678No Hit
GATTATTTATAGTGTGATTATTGCCTATAGTCTGATTAACTAACAATGGT26140.1011541009590678No Hit
GTGATGTTTTGATGTGAAGTGAAATTTTAGTTGTCGTAGTAGGCAAACAA26100.10099931274030871No Hit
ACTAATTACCATTATACTAGGACTTTACTTCACCATCCTCCAAGCTTCAG26030.1007284333574803No Hit
ATTATAGAAGAGCTAATAAATTTCCATGATCACACACTAATAATTGTTTT25990.1005736451387212No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGGGA200.002073896671.2541052
ACTTAAG301.2981733E-463.3664131
GGGTCAC452.0497828E-763.3284077
CGCACCT551.1847078E-860.449848
TAACTTT39950.058.0180176
GGTCGGG250.005011615357.029771
TCGGGAG250.005021223357.0021823
TGTATTG250.00502353156.9955645
GACGCAC602.354318E-855.4123546
AACTTGC3650.054.6532827
AACTTTA42750.054.5513087
ACCTTAA558.165298E-751.845251
GTATCCA558.1991857E-751.8141489
CTAGACG654.420872E-851.1558073
TATCAGG502.8292847E-547.4963048
CGCAGTG601.493816E-647.4963049
CGCAGAA2000.047.4963049
GTATCAG502.8292847E-547.4963047
TGGGTAT2300.045.4312486
ACTTTAT52600.044.3359038
Produced by FastQC (version 0.11.5)