FastQCFastQC Report
Fri 10 Feb 2017
SRR2031584_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031584_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466475
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25250.17218159191257948No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21680.1478375014916722No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17520.11947015803201555No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG44250.023.9952471
TACATGG42900.023.503722
ACATGGG41200.023.2952183
GTATCAA56300.023.0880831
CATGGGG25450.021.8436644
GGTATCA43100.021.32131
TCAACGC60900.020.7535214
CAACGCA61400.020.5845185
TCTATAC6250.020.5256233
AACGCAG63200.020.2148156
ATCAACG62850.020.108933
GAGTACT29900.019.9332212-13
TATCAAC64600.019.6578832
TAGCCCT10800.019.3578174
ATAGCCC5450.019.1795673
AGAGTAC48250.019.04565810-11
GTATAGC5300.018.865881
TATACTG9500.018.5057035
TCCGGTA1300.00750760818.292992
ACGCAGA70500.018.0536677
Produced by FastQC (version 0.11.5)