FastQCFastQC Report
Fri 10 Feb 2017
SRR2031584_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031584_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1466475
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28160.19202509418844507No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25840.17620484495132885No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18990.12949419526415384No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGGT700.00839694127.139796
CGTCACG458.956782E-426.38590610-11
GTATCAA56050.024.9294381
GGTATCA41650.024.7619881
GTACATG40750.024.25921
TACATGG40300.024.0435852
TATACTG8550.022.7752025
ACATGGG41100.022.6510523
TCAACGC61800.022.5960754
CAACGCA62300.022.4131975
ATCAACG63150.021.9625933
AACGCAG64100.021.9319976
TATCAAC66900.021.01552
GAGTACT35750.020.19352112-13
TCGCACG600.0047589519.79617922-23
ACGCAGA70300.019.795067
AGAGTAC51500.019.59729810-11
CGCAGAG71350.019.5037528
GGACCGT1759.9477795E-518.9978526
GTATAGC5300.018.8314691
Produced by FastQC (version 0.11.5)