FastQCFastQC Report
Fri 10 Feb 2017
SRR2031582_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031582_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2591179
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG62050.025.8772641
ACATGGG59500.023.0874793
TACATGG63900.023.0103912
CATGGGG31250.020.9914024
GTACCGA1656.317265E-520.1553966
GTATCAA63650.019.1633661
TCAACGC61350.019.1379224
TAGCCGC1250.006007611319.0139525
GGTATCA50350.018.8314531
CAACGCA62450.018.8008255
CATACCG2302.0454736E-618.6006055
ATCAACG63100.018.5311073
AACGCAG64850.018.2417556
GTATATA28850.018.0015931
GTATAGG10100.017.9263761
GAGTACA43800.017.8402121
CGCCGTA2400.017.81696394-95
TACACAG33700.017.7726865
TAAACCG2202.9011264E-517.2854125
CGTACAC2755.870479E-717.2847423
Produced by FastQC (version 0.11.5)