FastQCFastQC Report
Fri 10 Feb 2017
SRR2031582_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031582_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2591179
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG62250.024.2027991
ACATGGG59200.022.3074913
TAGATCG1353.8006576E-421.1086945
TACATGG66650.021.0256542
CATGGGG30300.019.4404684
TACACTG29500.018.8368245
GTGTAGC13400.018.4435371
GTATAGG11300.017.6651151
GGTATCA53750.017.5078321
GTATATA27450.017.4873771
GTATCAA66800.017.0757751
CCTACAC13450.016.9530013
TATACCG1700.001789751216.7627855
CCTATAC9750.016.5654033
TCAACGC64650.016.459144
GAGTACA43900.016.4560171
GTATAGC11650.016.3184811
CGCATAC1750.002169588816.2869914
CAACGCA65350.016.2796965
ATCAACG67150.015.91710953
Produced by FastQC (version 0.11.5)