FastQCFastQC Report
Fri 10 Feb 2017
SRR2031581_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031581_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022377
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA30150.034.454591
TCAACGC42750.029.918144
ATCAACG43900.029.1324713
CAACGCA44600.028.677145
AACGCAG45600.028.346486
GTATCAA49500.028.3021891
CGCAGAG49450.025.2729388
ACGCAGA51100.024.921437
TATCAAC54650.023.9513282
ATACCGT3600.022.4409646
ACATGGG48850.022.0928083
GCAGAGT59100.020.6638819
TACATGG61650.019.4560432
GTACATG62550.019.426331
GTCCTAC11400.019.2278561
CATGGGG23500.019.2209194
GACAGCG2803.4786353E-818.6678267
TATACCG2956.277696E-817.7291975
GTCGCCG5650.017.23607894-95
GTATTAC12850.016.6873471
Produced by FastQC (version 0.11.5)