FastQCFastQC Report
Fri 10 Feb 2017
SRR2031581_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031581_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3022377
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAACGC41200.034.12424
ATCAACG43000.032.6957443
CAACGCA43150.032.4709365
AACGCAG44200.032.0219356
GGTATCA29800.031.8899751
CGCAGAG47850.029.0830048
GTATCAA50300.029.0008561
ACGCAGA48950.028.7205417
TATCAAC55550.025.394542
GCAGAGT59600.022.8712169
ACATGGG45850.020.7185153
GTACATG60250.019.3218841
TACATGG59500.018.3602622
AGAGTAC58600.017.62849610-11
GCGCTAG1102.9083254E-517.28114344-45
CCTCGTC3903.0195224E-1017.0497118
GAGTACT35600.016.67683412-13
TAATACT33650.016.3734764
GTATAGG12800.016.3336471
CGTCCGA1752.5098416E-816.28421410-11
Produced by FastQC (version 0.11.5)