FastQCFastQC Report
Fri 10 Feb 2017
SRR2031580_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031580_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986681
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57530.2895784476722735No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40730.2050152993862628No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24170.12166019607576656No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA33800.052.980771
GTATCAA61950.043.2445531
TCAACGC73900.035.6089324
ATCAACG74750.035.267563
CAACGCA75350.035.0497675
AACGCAG75550.035.0198556
TATCAAC77800.034.1291772
ACGCAGA85300.031.0169987
CGCAGAG85600.030.9637878
GTACATG71400.027.9411751
TACATGG72050.027.4913252
GCAGAGT97500.026.7948729
ACATGGG71750.026.2822283
GAGTACT56350.023.93966312-13
TATACGT1000.001654068523.754
CATGGGG36300.023.2920114
CAGAGTA93300.022.70632410-11
AGAGTAC86000.022.14825610-11
TAAGGCG2806.548362E-1122.0535725
TCGCCGG3500.021.03571594-95
Produced by FastQC (version 0.11.5)