FastQCFastQC Report
Fri 10 Feb 2017
SRR2031580_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031580_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1986681
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT84870.42719490446629327No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69370.34917533313098575No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52540.2644611792230358No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30800.15503243852435292No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT26180.13177757274569998RNA PCR Primer, Index 26 (100% over 22bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31650.055.9069981
GTATCAA61750.044.9855271
ATCAACG77100.036.10173
TCAACGC77250.035.970114
CAACGCA78550.035.1329275
AACGCAG79550.034.8107036
TATCAAC81400.034.3113142
CGGACTA600.003952048731.6659959
ACGCAGA90550.030.476997
GTACATG67850.030.3553311
CGCAGAG90600.030.3553168
TACATGG68000.029.8265742
ACATGGG67050.028.8323523
GCAGAGT101050.026.6520829
CATGGGG34250.023.437464
GAGTACT62750.023.04931312-13
AGAGTAC90200.022.88061310-11
CATGGGA33850.022.591844
CAGAGTA97600.021.72981610-11
CGTATAG1353.7618322E-421.140461
Produced by FastQC (version 0.11.5)