FastQCFastQC Report
Fri 10 Feb 2017
SRR2031579_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031579_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091879
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46780.2236267011619697No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33610.16066894882543398No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.057.372421
GTATCAA53150.047.097731
TCAACGC64250.037.8520554
ATCAACG65250.037.4903343
CAACGCA65950.037.020395
AACGCAG67500.036.4517676
TATCAAC69000.035.59052
ACGCAGA77850.031.361527
CGCAGAG79900.030.4974278
GTACATG78650.028.6871571
TACATGG82450.026.9041212
ACATGGG79600.026.6739353
GCAGAGT89950.026.1394629
CATGGGG43300.023.4756984
AGAGTAC78550.022.07187710-11
CAGAGTA84900.021.59594710-11
GAGTACT46050.020.83600212-13
CATGGGA37400.019.9397964
GTACTTT49850.019.29534114-15
AGTACTT51350.019.1942112-13
Produced by FastQC (version 0.11.5)