FastQCFastQC Report
Fri 10 Feb 2017
SRR2031579_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031579_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091879
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53070.2536953619210289No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT49100.2347172087869327No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39640.1894947078679025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23610.11286503664886927No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28000.057.5813831
GTATCAA53850.046.6324961
TCAACGC65850.037.1476174
ATCAACG67650.036.159213
CAACGCA67650.036.0889975
TATCAAC70650.034.825482
AACGCAG70350.034.6364026
ACGCAGA80350.030.3257087
CGCAGAG81150.029.7926258
GTACATG73950.027.783441
TACATGG77450.025.8190442
ACATGGG75400.025.5130983
GCAGAGT93100.024.948179
AGAGTAC83000.021.68905610-11
GAGTACT50450.021.1365812-13
CAGAGTA90650.020.82806210-11
CATGGGG42000.020.808854
TCGCGCC757.727402E-418.99938428-29
GTACTTT55850.018.71022614-15
CATGGGA37550.018.5946034
Produced by FastQC (version 0.11.5)