FastQCFastQC Report
Fri 10 Feb 2017
SRR2031578_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031578_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979416
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45260.22865329976114165No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31340.1583295274969991No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24400.055.286891
GTATCAA49750.044.587941
TCAACGC57450.038.1984374
CAACGCA57850.038.0164265
ATCAACG58550.037.480793
AACGCAG59350.037.1356356
TATCAAC60750.036.3580252
ACGCAGA66850.032.6851167
CGCAGAG67300.032.043098
GCAGAGT78850.027.3493989
TACATGG58200.025.5455342
GTACATG61150.025.4006521
ACATGGG59500.023.8697473
CAGAGTA73100.022.90526610-11
GAGTACT46500.022.11559112-13
AGAGTAC69400.022.07312810-11
GTACTTT50800.020.00984214-15
CATGGGG34350.019.9126644
AGTACTT51250.019.23170912-13
GTATCGA1300.00756500918.2692329
Produced by FastQC (version 0.11.5)