FastQCFastQC Report
Fri 10 Feb 2017
SRR2031578_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031578_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1979416
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54440.2750306150905115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40970.2069802406366322No Hit
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT32530.16434140170636186No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24920.12589571873724373No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25150.055.8156051
GTATCAA49000.046.2255941
TCAACGC59650.037.584834
CAACGCA60700.037.0911835
ATCAACG60600.036.9172483
AACGCAG62450.036.2039156
TATCAAC62000.036.083632
ACGCAGA70150.032.0945787
CGCAGAG71100.031.6657498
GCAGAGT82350.027.1667929
GTACATG53100.026.4362071
TACATGG52350.026.1310482
ACATGGG51700.025.448973
AGAGTAC75650.022.28950710-11
CAGAGTA80100.021.34769810-11
GAGTACT56050.021.10108412-13
GCGTGCG3153.2196112E-1019.6026069
GTACTTT60700.019.60198614-15
AGTACTT59600.019.20665712-13
GTAATAC5200.019.1821373
Produced by FastQC (version 0.11.5)