FastQCFastQC Report
Fri 10 Feb 2017
SRR2031577_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031577_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376690
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45140.18992800912193006No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32900.13842781347167699No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27500.048.5363661
GTATCAA51100.042.5733871
TCAACGC60300.034.660034
ATCAACG60700.034.4316333
CAACGCA61900.033.8408745
AACGCAG62650.033.739036
TATCAAC65200.032.7837452
ACGCAGA71000.029.5704237
CGCAGAG71200.029.1537918
TACATGG58600.026.2627982
GTACATG61800.025.8252431
ACATGGG58200.025.1374593
GCAGAGT83900.024.1746129
CGCCGTA4150.021.74698894-95
AGAGTAC74050.021.52093110-11
GTATAGT9350.020.8288761
CAGAGTA78050.020.20499610-11
GAGTACT50300.019.83101512-13
CATGGGG39100.019.3158574
TCGCCGG1756.366463E-1119.094-95
Produced by FastQC (version 0.11.5)