##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2031577_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2376690 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.22718192107511 34.0 31.0 34.0 30.0 34.0 2 32.38284799447972 34.0 31.0 34.0 30.0 34.0 3 32.49136446065747 34.0 31.0 34.0 31.0 34.0 4 35.71266341003665 37.0 35.0 37.0 35.0 37.0 5 35.695269892160944 37.0 35.0 37.0 35.0 37.0 6 35.81811679268226 37.0 37.0 37.0 35.0 37.0 7 35.84469914040115 37.0 37.0 37.0 35.0 37.0 8 35.892965847460125 37.0 37.0 37.0 35.0 37.0 9 37.72596888950599 39.0 39.0 39.0 35.0 39.0 10-11 37.72459071229315 39.0 39.0 39.0 35.0 39.0 12-13 37.70229836453218 39.0 39.0 39.0 35.0 39.0 14-15 39.232330257627204 41.0 40.0 41.0 36.0 41.0 16-17 39.2432664756447 41.0 40.0 41.0 36.0 41.0 18-19 39.200406868375765 41.0 40.0 41.0 36.0 41.0 20-21 39.16402644013313 41.0 40.0 41.0 36.0 41.0 22-23 39.09604470923848 41.0 39.0 41.0 36.0 41.0 24-25 39.05030168006766 41.0 39.0 41.0 36.0 41.0 26-27 38.94900576011175 41.0 39.0 41.0 35.5 41.0 28-29 38.85030420458705 41.0 39.0 41.0 35.0 41.0 30-31 38.697700583584734 41.0 39.0 41.0 35.0 41.0 32-33 38.596198494544936 40.0 38.5 41.0 35.0 41.0 34-35 38.471186818642735 40.0 38.0 41.0 34.0 41.0 36-37 38.33841329748516 40.0 38.0 41.0 34.0 41.0 38-39 38.195722201885815 40.0 38.0 41.0 34.0 41.0 40-41 38.07869074216663 40.0 38.0 41.0 33.0 41.0 42-43 37.93151378597966 40.0 38.0 41.0 33.0 41.0 44-45 38.10533451985745 40.0 38.0 41.0 33.0 41.0 46-47 38.00061051294027 40.0 38.0 41.0 33.0 41.0 48-49 37.882037623754044 40.0 38.0 41.0 33.0 41.0 50-51 36.83907198667054 39.0 36.5 40.0 31.5 40.5 52-53 36.903128089906545 39.5 37.0 40.0 31.5 40.5 54-55 37.68988803756485 40.0 38.0 41.0 33.0 41.0 56-57 37.729132112307454 40.0 38.0 41.0 33.0 41.0 58-59 37.54811271137591 40.0 37.0 41.0 32.0 41.0 60-61 37.391993907493195 40.0 37.0 41.0 32.0 41.0 62-63 37.1496341550644 40.0 36.0 41.0 31.5 41.0 64-65 36.879707913106046 39.0 36.0 41.0 31.0 41.0 66-67 36.592434856880786 39.0 35.0 41.0 31.0 41.0 68-69 36.29351829645431 38.5 35.0 41.0 31.0 41.0 70-71 35.92897748549453 37.5 35.0 40.0 30.5 41.0 72-73 35.554547290559555 37.0 35.0 39.5 30.0 41.0 74-75 35.1609726131721 36.5 35.0 39.0 29.5 41.0 76-77 34.76817653964127 36.0 35.0 39.0 29.0 40.5 78-79 34.34628979799638 35.5 34.5 37.5 29.0 39.5 80-81 33.978563043560584 35.0 34.0 37.0 29.0 39.0 82-83 33.649139559639664 35.0 34.0 37.0 28.0 39.0 84-85 33.33371748103455 35.0 34.0 36.0 27.5 37.0 86-87 33.05455381223466 35.0 34.0 36.0 27.0 37.0 88-89 32.81136770045736 35.0 34.0 35.5 26.5 37.0 90-91 32.61524914902659 35.0 34.0 35.0 26.0 36.0 92-93 32.44553854309986 35.0 34.0 35.0 25.5 36.0 94-95 32.29876929679513 35.0 34.0 35.0 25.0 36.0 96-97 32.16621982673382 35.0 33.0 35.0 25.0 36.0 98-99 32.02096402980616 35.0 33.0 35.0 24.5 35.0 100-101 30.46770571677417 34.0 30.0 35.0 19.5 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2771973487697963 1101 2 0.18313125777819295 1101 3 0.33355176972188616 1101 4 0.06517309017564088 1101 5 0.14942283278184476 1101 6 0.10333828958616209 1101 7 0.12846374998784427 1101 8 0.19824851981015712 1101 9 0.3879970590754809 1101 10-11 0.4544191422918544 1101 12-13 0.5390428350027179 1101 14-15 0.5660914341667791 1101 16-17 0.5071442753469597 1101 18-19 0.6235326770868141 1101 20-21 0.6198670345195083 1101 22-23 0.5976016207246104 1101 24-25 0.5290379067335778 1101 26-27 0.49021511981047894 1101 28-29 0.5601484235224348 1101 30-31 0.6134477772438416 1101 32-33 0.6220538813609977 1101 34-35 0.5661130376409034 1101 36-37 0.5442369624247689 1101 38-39 0.6093258325523934 1101 40-41 0.4367020219722022 1101 42-43 0.5503555933543751 1101 44-45 0.6254670733000296 1101 46-47 0.6720739043204276 1101 48-49 0.4998600222420748 1101 50-51 0.6540541416642256 1101 52-53 0.6147922407742854 1101 54-55 0.6903962743128815 1101 56-57 0.6701303562336207 1101 58-59 0.6723376893411626 1101 60-61 0.5943472981253706 1101 62-63 0.6426640010544844 1101 64-65 0.7502255757185949 1101 66-67 0.6793314513202233 1101 68-69 0.5933670994064144 1101 70-71 0.5679050760346982 1101 72-73 0.6565997702199624 1101 74-75 0.5836980281029653 1101 76-77 0.6483075378895791 1101 78-79 0.6521032283674657 1101 80-81 0.6190512431782977 1101 82-83 0.5368801820380682 1101 84-85 0.49938923092322085 1101 86-87 0.5298442256364737 1101 88-89 0.4321975576193182 1101 90-91 0.408948912031768 1101 92-93 0.49395954753146754 1101 94-95 0.6428261246871685 1101 96-97 0.5555335884374557 1101 98-99 0.5773172777169364 1101 100-101 0.41204156051956176 1102 1 0.14264944108174404 1102 2 0.1751601936213092 1102 3 0.3012986254417953 1102 4 0.1142875582315881 1102 5 0.19994901842976276 1102 6 0.2001198960011763 1102 7 0.214361490836545 1102 8 0.24083051836148428 1102 9 0.34819616433814105 1102 10-11 0.38139988084659393 1102 12-13 0.41950106200368253 1102 14-15 0.49685142336220167 1102 16-17 0.4909310246874625 1102 18-19 0.48823792308583336 1102 20-21 0.5396353139441814 1102 22-23 0.5522627077135596 1102 24-25 0.5905460592178855 1102 26-27 0.5320237309518276 1102 28-29 0.5222198519978747 1102 30-31 0.604179558964482 1102 32-33 0.6124658260641382 1102 34-35 0.5499619401833371 1102 36-37 0.5557769642255366 1102 38-39 0.5071382414223606 1102 40-41 0.5375749888905759 1102 42-43 0.6064127420476737 1102 44-45 0.5919241660890506 1102 46-47 0.7200803655657211 1102 48-49 0.7993883970265614 1102 50-51 0.7162285092805973 1102 52-53 0.7893157440915886 1102 54-55 0.7591810549087228 1102 56-57 0.7601244970344823 1102 58-59 0.7147785833259448 1102 60-61 0.8132999259627454 1102 62-63 0.7914641418902875 1102 64-65 0.795606484136357 1102 66-67 0.7721214783182262 1102 68-69 0.8126241099516349 1102 70-71 0.747201945260322 1102 72-73 0.7719507375925545 1102 74-75 0.7044586757863627 1102 76-77 0.700378482577932 1102 78-79 0.7400009584653375 1102 80-81 0.5242750014245203 1102 82-83 0.5765083916630047 1102 84-85 0.6057814021731005 1102 86-87 0.6276693339174315 1102 88-89 0.6792316740157958 1102 90-91 0.670531245202497 1102 92-93 0.6340407700076796 1102 94-95 0.5361033655956717 1102 96-97 0.5716258923256277 1102 98-99 0.6792982514459496 1102 100-101 0.7179441661161725 1103 1 0.23406977859001898 1103 2 0.3304402942730391 1103 3 0.3979338045914105 1103 4 0.250921540164299 1103 5 0.11591828843744878 1103 6 0.19244488052821396 1103 7 0.2583405053346226 1103 8 0.24487593563966925 1103 9 0.37345156170191274 1103 10-11 0.4365947866886941 1103 12-13 0.4557959750077032 1103 14-15 0.4996678226682434 1103 16-17 0.5126968554758164 1103 18-19 0.5284374200409445 1103 20-21 0.5771987958357343 1103 22-23 0.5527228879588435 1103 24-25 0.5259273460764007 1103 26-27 0.5244681699327813 1103 28-29 0.4995876423737471 1103 30-31 0.5632740247224817 1103 32-33 0.4532635038909305 1103 34-35 0.530239929671346 1103 36-37 0.6070702257116025 1103 38-39 0.5906320944618457 1103 40-41 0.5748402355012203 1103 42-43 0.647048902487569 1103 44-45 0.6491781246546537 1103 46-47 0.6460776632761593 1103 48-49 0.6413496200263751 1103 50-51 0.6236579883950384 1103 52-53 0.661335662787728 1103 54-55 0.5685082297013153 1103 56-57 0.630664730470393 1103 58-59 0.7068235046142775 1103 60-61 0.6164413910355506 1103 62-63 0.6861213476929322 1103 64-65 0.8036890343793814 1103 66-67 0.7047868465746632 1103 68-69 0.7087398893772487 1103 70-71 0.640155254870237 1103 72-73 0.654762444235935 1103 74-75 0.5995359764470791 1103 76-77 0.5744187047899416 1103 78-79 0.5887951365705888 1103 80-81 0.5481009538800379 1103 82-83 0.5586714632939973 1103 84-85 0.558628426714975 1103 86-87 0.5840754063725697 1103 88-89 0.5133358938846868 1103 90-91 0.3976689454557345 1103 92-93 0.4049335410623982 1103 94-95 0.5673624850212562 1103 96-97 0.4577892718123877 1103 98-99 0.46319819618156544 1103 100-101 0.38147277051402284 1104 1 0.1092713866572197 1104 2 0.11814184237353942 1104 3 0.27740456242666056 1104 4 0.15286781958996443 1104 5 0.09480089281549908 1104 6 0.08880753997969038 1104 7 0.1351890390287167 1104 8 0.10712329590525371 1104 9 0.22904357701501254 1104 10-11 0.2970832035196693 1104 12-13 0.30249612708327334 1104 14-15 0.41928581076125226 1104 16-17 0.3510676320206798 1104 18-19 0.426463795797126 1104 20-21 0.36982361053861723 1104 22-23 0.3394189143250017 1104 24-25 0.42800387318855115 1104 26-27 0.3363096755288737 1104 28-29 0.3408274660236543 1104 30-31 0.3407478912317927 1104 32-33 0.3737989825931507 1104 34-35 0.38533086318506093 1104 36-37 0.40670778693760923 1104 38-39 0.432584802318452 1104 40-41 0.3607914753728423 1104 42-43 0.4318814034830183 1104 44-45 0.4729213424201575 1104 46-47 0.5925293133200569 1104 48-49 0.6000092921763951 1104 50-51 0.49308144449221913 1104 52-53 0.5186189790905189 1104 54-55 0.49867939866830113 1104 56-57 0.4609136162889911 1104 58-59 0.4797279080894654 1104 60-61 0.5001688255638967 1104 62-63 0.42993806065389606 1104 64-65 0.4937391185726412 1104 66-67 0.5460415419677815 1104 68-69 0.47667329275169124 1104 70-71 0.40082045355192975 1104 72-73 0.45560101865941505 1104 74-75 0.4639265468024192 1104 76-77 0.41416271891329615 1104 78-79 0.43932572198956876 1104 80-81 0.40460734153361244 1104 82-83 0.5041629264336578 1104 84-85 0.4647966021439842 1104 86-87 0.46722955918692577 1104 88-89 0.5127626121706896 1104 90-91 0.4728554419128983 1104 92-93 0.4286417382713026 1104 94-95 0.3266045538246587 1104 96-97 0.384871915528592 1104 98-99 0.4691291433197122 1104 100-101 0.5162455446698075 1105 1 0.16948866665008921 1105 2 0.15924694266517037 1105 3 0.23597443263748374 1105 4 0.26468464543203396 1105 5 0.13936110119057332 1105 6 0.0872558580633509 1105 7 0.16465986429196988 1105 8 0.16907703485758674 1105 9 0.30290986967513334 1105 10-11 0.2465266461608664 1105 12-13 0.3269646148056182 1105 14-15 0.3025401129496359 1105 16-17 0.2905498110540563 1105 18-19 0.3315206759591973 1105 20-21 0.31097434853366934 1105 22-23 0.3369878717817514 1105 24-25 0.33903261381055927 1105 26-27 0.2590127633090944 1105 28-29 0.282283197451342 1105 30-31 0.29691343365259826 1105 32-33 0.30704326674907634 1105 34-35 0.25713232804402963 1105 36-37 0.2954586496015139 1105 38-39 0.3959922690919697 1105 40-41 0.3307106632443464 1105 42-43 0.4000452094448619 1105 44-45 0.3410573205140537 1105 46-47 0.383406225094582 1105 48-49 0.389213192530363 1105 50-51 0.3983141967796655 1105 52-53 0.31051216460222264 1105 54-55 0.3547892853532275 1105 56-57 0.35898904094225514 1105 58-59 0.4709799395238221 1105 60-61 0.4036473090564101 1105 62-63 0.4104407432603665 1105 64-65 0.4154481949100486 1105 66-67 0.42432244759791615 1105 68-69 0.38039109590848597 1105 70-71 0.35080375683116216 1105 72-73 0.3016583663296686 1105 74-75 0.32121967443215027 1105 76-77 0.3110393190157055 1105 78-79 0.3293755895289152 1105 80-81 0.4007593039548638 1105 82-83 0.39685532831258996 1105 84-85 0.39774813712917734 1105 86-87 0.4253207900691862 1105 88-89 0.4358085392839399 1105 90-91 0.2813195662255481 1105 92-93 0.39448136070279105 1105 94-95 0.3411318140040933 1105 96-97 0.32299275526234084 1105 98-99 0.31480469264182176 1105 100-101 0.3938396585191839 1106 1 0.2242669054890527 1106 2 0.1647831521862031 1106 3 0.12497708084316628 1106 4 0.17924769149413322 1106 5 0.1749700842378843 1106 6 0.053371336512796574 1106 7 0.13696839068019528 1106 8 0.07382712347864384 1106 9 0.11067515941385864 1106 10-11 0.1402095287431777 1106 12-13 0.2091184192554323 1106 14-15 0.21884534471976025 1106 16-17 0.22231055505388753 1106 18-19 0.22513474689774426 1106 20-21 0.2882018648503717 1106 22-23 0.24010420516373898 1106 24-25 0.3113546940073988 1106 26-27 0.2932653228937241 1106 28-29 0.27731729049302345 1106 30-31 0.3139745509034668 1106 32-33 0.2269194057902979 1106 34-35 0.23765341985544808 1106 36-37 0.2829182488258155 1106 38-39 0.3713775100369858 1106 40-41 0.40287948114371375 1106 42-43 0.3585246806377995 1106 44-45 0.4522412978270651 1106 46-47 0.45537277846530344 1106 48-49 0.36000994795710994 1106 50-51 0.3373335265959554 1106 52-53 0.31258798252243736 1106 54-55 0.3003363379211521 1106 56-57 0.38672951678520207 1106 58-59 0.4260282119345291 1106 60-61 0.47576503952328864 1106 62-63 0.44277387502424403 1106 64-65 0.48362698007175453 1106 66-67 0.43844760138151173 1106 68-69 0.2899288067744692 1106 70-71 0.4686708043946055 1106 72-73 0.4475567753210541 1106 74-75 0.46209691863007407 1106 76-77 0.426656434348196 1106 78-79 0.49754811908665175 1106 80-81 0.4695438401018279 1106 82-83 0.4452486806907672 1106 84-85 0.45136074842679363 1106 86-87 0.5351848349427186 1106 88-89 0.5175651128811154 1106 90-91 0.6101016001309176 1106 92-93 0.4673841694689784 1106 94-95 0.47254745718444013 1106 96-97 0.4799448164838438 1106 98-99 0.4758541744692977 1106 100-101 0.5127753717622987 1107 1 0.3088916802717563 1107 2 0.22436376383822676 1107 3 0.2475017447045218 1107 4 0.2632401950105532 1107 5 0.22728270638341996 1107 6 0.21528736102895607 1107 7 0.21728268740557155 1107 8 0.2622371138341606 1107 9 0.3504030010643362 1107 10-11 0.281482346360697 1107 12-13 0.32242037377963584 1107 14-15 0.4316413061652966 1107 16-17 0.3079173848355836 1107 18-19 0.36765723928290583 1107 20-21 0.35669648408887156 1107 22-23 0.3036475652394941 1107 24-25 0.41289174702076537 1107 26-27 0.3469433340562844 1107 28-29 0.41794169749417165 1107 30-31 0.38437371126600084 1107 32-33 0.3809241165242412 1107 34-35 0.3823871988304717 1107 36-37 0.360924031603858 1107 38-39 0.37585065318030075 1107 40-41 0.39808540946934556 1107 42-43 0.44918342043822435 1107 44-45 0.44576951625800376 1107 46-47 0.515405683176624 1107 48-49 0.3855804495289803 1107 50-51 0.33763303380780485 1107 52-53 0.3533085409243881 1107 54-55 0.3707775584081503 1107 56-57 0.3524425923975585 1107 58-59 0.2737296750399736 1107 60-61 0.34312921955626763 1107 62-63 0.3965004020512879 1107 64-65 0.37181483428157946 1107 66-67 0.35473719414169125 1107 68-69 0.3540638452056797 1107 70-71 0.382786279977239 1107 72-73 0.22192181416308898 1107 74-75 0.31012948294782916 1107 76-77 0.2942535100935899 1107 78-79 0.23270529679360408 1107 80-81 0.34402537570719005 1107 82-83 0.30307831958014475 1107 84-85 0.3484203544701501 1107 86-87 0.2631918893059151 1107 88-89 0.29315322101672336 1107 90-91 0.24687281066042743 1107 92-93 0.24436759777994865 1107 94-95 0.22937528259775775 1107 96-97 0.3447076267261693 1107 98-99 0.29421420130952214 1107 100-101 0.3408052168440996 1108 1 0.0798832348610361 1108 2 0.10702868945435995 1108 3 0.035429072290632746 1108 4 -0.08066479421020745 1108 5 0.020557076431209964 1108 6 0.10216055634273857 1108 7 0.1347059472412795 1108 8 0.12674343793342047 1108 9 0.20235632892308075 1108 10-11 0.15047505507205727 1108 12-13 0.2025324712667711 1108 14-15 0.17976722826607983 1108 16-17 0.20973286125447288 1108 18-19 0.2122462779006753 1108 20-21 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-0.5666543414612946 2316 80-81 -0.4147532828805467 2316 82-83 -0.4791631965744898 2316 84-85 -0.3693528498713121 2316 86-87 -0.4541310291756062 2316 88-89 -0.44120171474544634 2316 90-91 -0.3924345032886336 2316 92-93 -0.25173671367187467 2316 94-95 -0.33807927341594635 2316 96-97 -0.2597590960800318 2316 98-99 -0.2392325806690394 2316 100-101 -0.21335148828137562 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 46.0 7 1084.0 8 1969.0 9 1574.0 10 1697.0 11 2216.0 12 2832.0 13 3095.0 14 3372.0 15 3558.0 16 4099.0 17 5226.0 18 6609.0 19 8410.0 20 10387.0 21 13095.0 22 14942.0 23 14598.0 24 13928.0 25 13312.0 26 13967.0 27 15547.0 28 17543.0 29 20757.0 30 25197.0 31 31316.0 32 39277.0 33 52189.0 34 75848.0 35 120162.0 36 225326.0 37 498693.0 38 814945.0 39 299592.0 40 282.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.64763179043123 19.911936348450997 13.662530662391815 23.77790119872596 2 14.497683753455437 21.447853948137958 41.43805039782218 22.616411900584428 3 17.172117524792885 27.303350457989893 31.420462912706327 24.104069104510895 4 11.689366303556627 18.29203640356967 40.964997538593586 29.053599754280114 5 12.399429458616815 38.61466998220214 36.490918041477855 12.494982517703193 6 28.41342371112766 39.55963966693174 19.564057575872326 12.462879046068272 7 25.14383449250849 33.682853043518506 24.41357518229134 16.759737281681666 8 24.260967984886545 36.12414744876278 22.03097585297199 17.58390871337869 9 25.84868030748646 16.160963356600988 21.275555499455123 36.71480083645743 10-11 23.08813938713084 26.817022834277925 30.898497490206967 19.196340288384263 12-13 24.402320033323658 24.772435614236606 29.920835279317032 20.904409073122704 14-15 21.73169407873976 26.15719761517068 27.532156065788975 24.578952240300588 16-17 21.27974199411787 29.006265015630984 28.070068035797686 21.64392495445346 18-19 21.22359247524919 28.432504870218665 29.59736019421969 20.74654246031245 20-21 22.189200106029812 27.762791950149158 29.30319057176157 20.744817372059458 22-23 21.836672010232718 27.659055240691888 28.949778894176355 21.55449385489904 24-25 21.504929124117997 27.880855307170897 29.145744712183753 21.468470856527354 26-27 21.59680059242055 28.02957053717565 28.99479949004708 21.378829380356716 28-29 21.614619491814246 27.97384177153941 29.114104069104513 21.297434667541832 30-31 21.927659896747155 27.885651894020675 28.96806062212573 21.218627587106436 32-33 21.724309859510495 27.976976383121066 28.926995106639907 21.371718650728535 34-35 21.879841291880727 28.053637622071033 28.785958623127122 21.28056246292112 36-37 21.93927268596241 27.789951571302947 28.853847157180784 21.41692858555386 38-39 21.681203690847354 28.09508181546605 28.874863781141 21.3488507125456 40-41 21.84193142563818 27.86516121160101 28.88256356529459 21.410343797466226 42-43 21.71048811582495 27.844249775948903 28.865670323012253 21.57959178521389 44-45 21.790767832573874 28.036723342127072 28.584417824789938 21.58809100050911 46-47 21.730592797499547 27.95486240624297 28.484506909508756 21.830037886748727 48-49 21.620151256865917 28.03669227586065 28.65738861957125 21.685767847702188 50-51 21.62210889935162 28.184260463081007 28.444454262019867 21.749176375547506 52-53 21.688798286692837 28.093525028506033 28.428865354758088 21.788811330043043 54-55 21.69948541879673 27.94935393341159 28.461873445842752 21.889287201948928 56-57 21.701336733019453 28.08184912630591 28.28414728046148 21.932666860213153 58-59 21.68126680383222 27.974283562433467 28.34675536144806 21.997694272286246 60-61 21.747198835355054 28.030643457918362 28.313200291161237 21.908957415565343 62-63 21.580180839739302 27.978322793464862 28.42350075104452 22.017995615751317 64-65 21.622866255170006 27.74573040657385 28.509523749416203 22.121879588839942 66-67 21.58154828774472 27.995826127934226 28.161876391115374 22.260749193205676 68-69 21.636141019653383 27.93256587943737 28.215080637356998 22.21621246355225 70-71 21.712065940446585 27.716803621843827 28.20710736360232 22.364023074107266 72-73 21.603132928568726 27.758647530809654 28.257576713833103 22.380642826788517 74-75 21.74198149527284 27.855652188547936 28.03196883060054 22.370397485578682 76-77 21.711434810597932 27.772511349818448 28.09830057769419 22.417753261889434 78-79 21.752689665038353 27.849130513445168 28.013203236433863 22.384976585082615 80-81 21.73813160319604 27.84774202777813 28.014002667575493 22.400123701450337 82-83 21.887541076034317 27.862152825989085 27.966815192557714 22.283490905418883 84-85 22.001922842272236 27.814797049678337 27.79594730486517 22.38733280318426 86-87 21.878452806213684 27.74127042231002 27.93727831564066 22.442998455835635 88-89 22.016796469038873 27.621671315989886 27.853148706814938 22.508383508156303 90-91 22.087903765320675 27.643571521738213 27.925539300455675 22.34298541248543 92-93 22.151037787847805 27.52658108545919 27.87044166466809 22.451939462024917 94-95 22.088177254921764 27.4329424535804 27.840547147503457 22.63833314399438 96-97 22.163176518603603 27.382809705935568 27.94859657759321 22.505417197867622 98-99 22.235525036921096 27.32116935738359 27.83686555671964 22.606440048975678 100-101 22.589083978137662 27.193428676015802 27.766200051331897 22.45128729451464 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 64.0 1 46.0 2 45.0 3 75.0 4 95.0 5 109.0 6 138.0 7 269.5 8 536.0 9 738.5 10 735.0 11 726.0 12 848.5 13 997.5 14 1089.5 15 1184.5 16 1342.0 17 1524.5 18 1703.0 19 1946.0 20 2304.5 21 2784.5 22 3463.5 23 4524.0 24 5945.5 25 7930.0 26 11242.5 27 15693.5 28 20581.5 29 27281.5 30 34561.5 31 40999.5 32 48301.0 33 55879.0 34 62817.0 35 68215.5 36 73384.5 37 77904.0 38 80763.0 39 83379.5 40 85587.5 41 87514.5 42 88453.5 43 90393.5 44 92943.5 45 94163.5 46 95218.0 47 95898.5 48 95577.5 49 93955.0 50 90222.0 51 83846.0 52 77371.0 53 72262.5 54 66378.5 55 58146.0 56 49989.0 57 43345.0 58 37709.5 59 32362.0 60 26509.5 61 20676.5 62 15397.5 63 11152.5 64 7940.0 65 5672.0 66 4066.0 67 3039.0 68 2296.0 69 1676.0 70 1220.5 71 931.0 72 693.5 73 470.5 74 350.0 75 243.0 76 192.0 77 144.0 78 95.0 79 74.5 80 70.5 81 61.5 82 50.0 83 39.5 84 24.5 85 22.5 86 19.5 87 15.0 88 12.5 89 10.0 90 11.0 91 9.0 92 6.5 93 5.0 94 5.5 95 4.5 96 4.0 97 4.0 98 2.0 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 6.311298486550623E-5 48-49 1.893389545965187E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2376690.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.7991430948921 #Duplication Level Percentage of deduplicated Percentage of total 1 64.19358276191886 30.042046654488395 2 16.253190798562986 15.212708038610657 3 7.300132376122977 10.249198190455017 4 3.8513411197157197 7.209578566752718 5 2.34907778858098 5.496741378441699 6 1.5334563602912066 4.305866618102436 7 1.0624082535234503 3.4803857117286854 8 0.7391016106013605 2.767145762895863 9 0.5356477142587529 2.256106862524253 >10 2.1122540955535842 15.54946457792233 >50 0.051956723674658714 1.6398378520479064 >100 0.017488597641236666 1.3434940878855075 >500 9.044988853943073E-5 0.030308117300984833 >1k 2.71349665618292E-4 0.41711758084344186 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4514 0.18992800912193006 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3290 0.13842781347167699 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.207532324367082E-5 7 0.0 0.0 0.0 0.0 4.207532324367082E-5 8 0.0 0.0 0.0 0.0 8.415064648734165E-5 9 0.0 0.0 0.0 0.0 8.415064648734165E-5 10-11 0.0 0.0 0.0 6.311298486550623E-5 8.415064648734165E-5 12-13 0.0 0.0 0.0 1.2622596973101247E-4 1.2622596973101247E-4 14-15 0.0 0.0 0.0 1.2622596973101247E-4 1.683012929746833E-4 16-17 0.0 0.0 0.0 2.103766162183541E-4 2.5245193946202494E-4 18-19 0.0 0.0 0.0 2.7348960108386034E-4 2.5245193946202494E-4 20-21 0.0 0.0 0.0 3.9971557081487283E-4 2.9452726270569573E-4 22-23 0.0 0.0 0.0 7.994311416297456E-4 2.9452726270569573E-4 24-25 0.0 0.0 0.0 0.0014515986519066434 2.9452726270569573E-4 26-27 0.0 0.0 0.0 0.002629707702729426 3.1556492432753113E-4 28-29 0.0 0.0 0.0 0.008036386739541126 3.366025859493666E-4 30-31 0.0 0.0 0.0 0.026654717274865465 3.366025859493666E-4 32-33 0.0 0.0 0.0 0.07011852618557742 3.366025859493666E-4 34-35 0.0 0.0 0.0 0.13409405517757889 3.366025859493666E-4 36-37 0.0 0.0 0.0 0.23989245547378918 3.366025859493666E-4 38-39 0.0 0.0 0.0 0.39430889177806105 3.366025859493666E-4 40-41 0.0 0.0 0.0 0.5596649121256874 3.366025859493666E-4 42-43 0.0 0.0 0.0 0.7200981196538043 3.366025859493666E-4 44-45 0.0 0.0 0.0 0.9145913013476726 3.366025859493666E-4 46-47 0.0 0.0 0.0 1.15137018290143 3.366025859493666E-4 48-49 0.0 0.0 0.0 1.3892009475362794 3.366025859493666E-4 50-51 0.0 0.0 0.0 1.6304818886771097 4.207532324367082E-4 52-53 2.103766162183541E-5 0.0 0.0 1.8635371041238025 4.207532324367082E-4 54-55 4.207532324367082E-5 0.0 0.0 2.1164939474647513 4.207532324367082E-4 56-57 4.207532324367082E-5 0.0 0.0 2.413819219166151 4.207532324367082E-4 58-59 4.207532324367082E-5 0.0 0.0 2.736600061429972 4.207532324367082E-4 60-61 4.207532324367082E-5 0.0 0.0 3.050797537752084 4.62828555680379E-4 62-63 4.207532324367082E-5 0.0 0.0 3.3721478190256198 5.890545254113915E-4 64-65 4.207532324367082E-5 0.0 0.0 3.7022918428570826 5.890545254113915E-4 66-67 4.207532324367082E-5 0.0 0.0 4.072891289987335 5.890545254113915E-4 68-69 4.207532324367082E-5 0.0 0.0 4.482136921516899 5.890545254113915E-4 70-71 4.207532324367082E-5 0.0 0.0 4.880127404078782 5.890545254113915E-4 72-73 4.207532324367082E-5 0.0 0.0 5.292297270573782 7.363181567642393E-4 74-75 4.207532324367082E-5 0.0 0.0 5.714943892556454 9.046194497389227E-4 76-77 4.207532324367082E-5 0.0 0.0 6.165633717481034 9.25657111360758E-4 78-79 4.207532324367082E-5 0.0 0.0 6.6483849387172915 9.466947729825934E-4 80-81 4.207532324367082E-5 0.0 0.0 7.14674610487695 9.677324346044288E-4 82-83 4.207532324367082E-5 0.0 0.0 7.660401651035684 9.677324346044288E-4 84-85 4.207532324367082E-5 0.0 0.0 8.188825635652947 9.677324346044288E-4 86-87 4.207532324367082E-5 0.0 0.0 8.74087491427153 9.887700962262643E-4 88-89 4.207532324367082E-5 0.0 0.0 9.35891933739781 0.0010098077578480997 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2750 0.0 48.536366 1 GTATCAA 5110 0.0 42.573387 1 TCAACGC 6030 0.0 34.66003 4 ATCAACG 6070 0.0 34.431633 3 CAACGCA 6190 0.0 33.840874 5 AACGCAG 6265 0.0 33.73903 6 TATCAAC 6520 0.0 32.783745 2 ACGCAGA 7100 0.0 29.570423 7 CGCAGAG 7120 0.0 29.153791 8 TACATGG 5860 0.0 26.262798 2 GTACATG 6180 0.0 25.825243 1 ACATGGG 5820 0.0 25.137459 3 GCAGAGT 8390 0.0 24.174612 9 CGCCGTA 415 0.0 21.746988 94-95 AGAGTAC 7405 0.0 21.520931 10-11 GTATAGT 935 0.0 20.828876 1 CAGAGTA 7805 0.0 20.204996 10-11 GAGTACT 5030 0.0 19.831015 12-13 CATGGGG 3910 0.0 19.315857 4 TCGCCGG 175 6.366463E-11 19.0 94-95 >>END_MODULE