FastQCFastQC Report
Fri 10 Feb 2017
SRR2031577_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2031577_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376690
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT68820.2895623745629426No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56980.2397451918424363No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42170.17743163811855983No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24020.10106492643129732No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28750.049.637411
GTATCAA55400.045.9377441
TCAACGC63750.038.5208634
ATCAACG63950.038.4746633
CAACGCA66450.037.170125
AACGCAG67050.037.120876
TATCAAC69450.035.974862
ACGCAGA75500.032.714637
CGCAGAG75650.032.46148
GCAGAGT89500.026.9073289
GTACATG57600.023.8671761
TACATGG55900.023.6221052
GAGTACT55050.022.9514412-13
AGAGTAC79550.022.39116510-11
ACATGGG55900.022.0926173
CAGAGTA85300.021.4943310-11
AGTACTT61400.019.84287612-13
GTACTTT63400.019.59151614-15
CATGGGG34700.019.3007984
GTACCGT2001.2858813E-518.9996516
Produced by FastQC (version 0.11.5)